Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,003 G→T 8.4% intergenic (+41/+69) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,0030GT 8.4% 188.9 / 7.4 79intergenic (+41/+69)ypfN/ypfHputative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base T (2/5);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

CATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGT  >  NC_000913/2592855‑2593149
                                                                                                                                                    |                                                                                                                                                  
cATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                                                                                                                    >  1:565133/1‑149 (MQ=255)
cATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                                                                                                                    <  2:459749/149‑1 (MQ=255)
    aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                                                                                                                >  2:267153/1‑149 (MQ=255)
     gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                               >  2:101918/1‑149 (MQ=255)
     gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCCACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                               >  1:876514/1‑149 (MQ=255)
      taaTTTCTCGCGAAGATGCCGGCGCTTCCCCCGCATCGTGATTTCAAGGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  2:250393/148‑1 (MQ=255)
      aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:1020306/149‑1 (MQ=255)
      aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:427793/149‑1 (MQ=255)
      aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:722534/149‑1 (MQ=255)
       aaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                                                             <  1:162974/149‑1 (MQ=255)
           tctcGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                                         >  2:290592/1‑149 (MQ=255)
               gCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                                     >  1:36250/1‑149 (MQ=255)
                 caacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                                                                                                                   <  1:975988/149‑1 (MQ=255)
                  aacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                                  <  2:930144/149‑1 (MQ=255)
                  aacaaGCCGGAGCTTCCCACGCATCGTGATATCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                                  <  2:958983/149‑1 (MQ=255)
                   acaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACg                                                                                                                                 >  2:868850/1‑149 (MQ=255)
                        ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                                                                                                            <  2:672868/149‑1 (MQ=255)
                         cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                                                                                                           >  1:1081007/1‑149 (MQ=255)
                          ggAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                                                                                                                          <  1:748523/149‑1 (MQ=255)
                                tCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCgc                                                                                                                    >  1:292134/1‑148 (MQ=255)
                                 cccccGCATCGTGATTTCAACGATTAGTGGGACGATGACGACGACTGGCCGAATATTTATCAACCGAAGAAGTAAGCTGAGTGCTCAAGCGTAGGCCGGATAAGTGGTTAACGCGTCATCCGGCAATGGTGATGGATGCCTGATGCGAc                                                                                                                   <  1:147842/149‑1 (MQ=255)
                                    ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGAAATGGTGAACGATGCCTGATGCGACGCt                                                                                                                >  2:387791/1‑149 (MQ=255)
                                        ctCGTGATCTCAACGATAAGTGGGACGATGCCGACCACTCGCCGAAAAAGGATCAACCGCAGACGTAAGCAGAGTGCTCAACCGTGGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg                                                                                                            <  1:818348/148‑1 (MQ=37)
                                              aTTTCAACGATAAGTGGGACGATGACGCCGAGTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGTCCGGATAAGGCGTTAACGCCGCATCCTGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCtt                                                                                                      <  1:881735/149‑1 (MQ=255)
                                                ttCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt                                                                                                    >  1:287589/1‑149 (MQ=255)
                                                 tCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   >  1:15416/1‑149 (MQ=255)
                                                 tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:866541/149‑1 (MQ=255)
                                                 tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:455385/149‑1 (MQ=255)
                                                 tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:540608/149‑1 (MQ=255)
                                                       aTAAGTGCGACGATGACGACGACTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa                                                                                             <  1:733778/149‑1 (MQ=255)
                                                        ttaGTGGTACGATGAGGACGACTTGCCGATAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAAACGGAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGAGACGCTCACGCGTCTTATCAgtcaag                                                                                            <  2:264485/147‑6 (MQ=37)
                                                        tAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTACGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg                                                                                            <  2:620018/149‑1 (MQ=255)
                                                             gtgACGATGACGACGCCTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCGCACGCGTCTTATCAGGCCAGGTAAt                                                                                       <  2:1039148/147‑1 (MQ=255)
                                                             gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt                                                                                       <  1:101918/149‑1 (MQ=255)
                                                              ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc                                                                                      <  1:267153/149‑1 (MQ=255)
                                                                  gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTc                                                                                  >  1:225077/1‑149 (MQ=255)
                                                                   ctGACGACGACTGGCCGAAACAGGATCAACCGAAGAAGTCAGCTGAGTGCTCAACCGTAGGCCGGAAAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCATGATGCGACTCTCACGCGTCTTATCAGGCCAGGGAATCTGTCt                                                                                 <  1:755823/148‑1 (MQ=38)
                                                                     tacgaccacTGGCCGTATTAGGTTCAAGCGATGAAGTAAGCTGAGTGCTCAACAGTAGGCCGGATATGGCGTTAACGCCGCAGCCGGCAATGGTGATCGATGCCTGCTGGGACGCTGACGCGTCTTATCAGGCCAGGTATGCTGTCttt                                                                               <  1:231848/148‑1 (MQ=25)
                                                                     gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               <  1:273385/149‑1 (MQ=255)
                                                                     gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               >  1:983041/1‑149 (MQ=255)
                                                                     gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               >  1:901211/1‑149 (MQ=255)
                                                                      acgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                              <  2:1001535/149‑1 (MQ=255)
                                                                      acgacgacTGGCCGAAAAAGGATAAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                              <  1:360444/149‑1 (MQ=255)
                                                                        gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAc                                                                            >  1:332156/1‑149 (MQ=255)
                                                                         acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca                                                                           >  1:179752/1‑149 (MQ=255)
                                                                          cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat                                                                          <  2:131388/149‑1 (MQ=255)
                                                                           gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCGGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcaaa                                                                         >  2:1026283/1‑147 (MQ=255)
                                                                           gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatc                                                                         >  1:242666/1‑149 (MQ=255)
                                                                                   gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGGGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCCGGTATTCTGTCTTTTACATCATTTCAAt                                                                 >  2:806506/1‑149 (MQ=255)
                                                                                    aaaaaGGATCAAGCGAAGAAGTAAGCTGATTGCTCAACCGTAGGCCGGATAAGGAGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATc                                                                <  1:569104/149‑1 (MQ=255)
                                                                                     aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATca                                                               >  2:188084/1‑149 (MQ=255)
                                                                                      aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                              <  1:246404/149‑1 (MQ=255)
                                                                                          gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatca                                                          >  1:274173/1‑149 (MQ=255)
                                                                                            tCACCCGACTAAGGAAGCTGAGGGCACCACCGGAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                        <  2:664265/149‑1 (MQ=25)
                                                                                            tCAACCGAAGAAGTAAGCTGAGTGCTCAACCTTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                        >  2:246888/1‑149 (MQ=255)
                                                                                            tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCTTTTCAATCAcatcatc                                                        <  1:619711/149‑1 (MQ=255)
                                                                                             cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                                       >  2:1174873/1‑149 (MQ=255)
                                                                                             cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                                       <  1:867141/149‑1 (MQ=255)
                                                                                              aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatcat                                                      >  2:438099/1‑149 (MQ=255)
                                                                                                ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCg                                                    <  2:174560/149‑1 (MQ=255)
                                                                                                  gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGcc                                                  <  2:233039/149‑1 (MQ=255)
                                                                                                     taagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAgg                                               <  2:472364/148‑1 (MQ=255)
                                                                                                         tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCCTCGCCAGGCTTa                                           >  2:974302/1‑149 (MQ=255)
                                                                                                               gAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCACCATCGCCAGGCTTACCGCCa                                     >  2:180247/1‑149 (MQ=255)
                                                                                                                 gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACt                                   <  2:36250/149‑1 (MQ=255)
                                                                                                                    cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATATCAATCACATCCTCATCGCCAGGCTTACCGCCACTTAg                                >  2:564899/1‑149 (MQ=255)
                                                                                                                     tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                               >  1:508811/1‑149 (MQ=255)
                                                                                                                     tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                               >  1:754742/1‑149 (MQ=255)
                                                                                                                      cAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcg                              >  2:1142003/1‑149 (MQ=255)
                                                                                                                       aaCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcgc                             >  1:217070/1‑149 (MQ=255)
                                                                                                                        aCCGTAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCt                            <  1:793643/149‑1 (MQ=255)
                                                                                                                         ccGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCCAAGGTGAACGCTGCCTGATGCGACGCTCACGCCTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAAATCATCATCTCCAGGCTTACCGCCACTTTGAGCtt                           >  1:640427/1‑149 (MQ=37)
                                                                                                                          cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCCCATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtc                          >  1:469614/1‑149 (MQ=255)
                                                                                                                          cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCCGGCTTCCCGCCCCGTAGCGCttg                          >  2:90369/1‑148 (MQ=255)
                                                                                                                               gCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGa                     >  1:1061403/1‑149 (MQ=255)
                                                                                                                                ccGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa                    >  1:945501/1‑149 (MQ=255)
                                                                                                                                 cGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaaa                   >  1:559423/1‑149 (MQ=255)
                                                                                                                                 cGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTGTTTTACATCATATCAATCACATAATCATCGCCAGGCTTACCGCAACTTAGCTCTTCCTCGaaa                   >  2:119251/1‑149 (MQ=37)
                                                                                                                                    aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataa                <  1:552768/149‑1 (MQ=255)
                                                                                                                                     tatggCGTTAACGCAGCATCCGGCAATGGTGATCGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataat               <  1:339184/146‑1 (MQ=255)
                                                                                                                                         gCGTTAACGCCGCATCCGGCAATGGTGAACGATCCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGCTAATCTGTCTTTTACATCAATTCAATCACATCATAATCGCCACGCTTACCGCCACTTAGCGCTTCGACGAAATAATGttt           >  2:763076/1‑149 (MQ=38)
                                                                                                                                                cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATg    >  2:41722/1‑149 (MQ=255)
                                                                                                                                                  ccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGt  >  1:474798/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGT  >  NC_000913/2592855‑2593149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: