Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,593,004 C→T 7.6% intergenic (+42/+68) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,593,0040CT 7.6% 225.1 / 7.5 79intergenic (+42/+68)ypfN/ypfHputative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base T (2/4);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

AGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGT  >  NC_000913/2592859‑2593149
                                                                                                                                                 |                                                                                                                                                 
aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                                                                                                                >  2:267153/1‑149 (MQ=255)
 gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                               >  2:101918/1‑149 (MQ=255)
 gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCCACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                               >  1:876514/1‑149 (MQ=255)
  taaTTTCTCGCGAAGATGCCGGCGCTTCCCCCGCATCGTGATTTCAAGGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCGCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  2:250393/148‑1 (MQ=255)
  aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:1020306/149‑1 (MQ=255)
  aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:427793/149‑1 (MQ=255)
  aaaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                                                                              <  1:722534/149‑1 (MQ=255)
   aaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                                                             <  1:162974/149‑1 (MQ=255)
       tctcGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAAt                                                                                                                                         >  2:290592/1‑149 (MQ=255)
           gCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                                     >  1:36250/1‑149 (MQ=255)
             caacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGaa                                                                                                                                   <  1:975988/149‑1 (MQ=255)
              aacaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                                  <  2:930144/149‑1 (MQ=255)
              aacaaGCCGGAGCTTCCCACGCATCGTGATATCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc                                                                                                                                  <  2:958983/149‑1 (MQ=255)
               acaaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACg                                                                                                                                 >  2:868850/1‑149 (MQ=255)
                    ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGcc                                                                                                                            <  2:672868/149‑1 (MQ=255)
                     cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCt                                                                                                                           >  1:1081007/1‑149 (MQ=255)
                      ggAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                                                                                                                          <  1:748523/149‑1 (MQ=255)
                            tCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCgc                                                                                                                    >  1:292134/1‑148 (MQ=255)
                             cccccGCATCGTGATTTCAACGATTAGTGGGACGATGACGACGACTGGCCGAATATTTATCAACCGAAGAAGTAAGCTGAGTGCTCAAGCGTAGGCCGGATAAGTGGTTAACGCGTCATCCGGCAATGGTGATGGATGCCTGATGCGAc                                                                                                                   <  1:147842/149‑1 (MQ=255)
                                ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGAAATGGTGAACGATGCCTGATGCGACGCt                                                                                                                >  2:387791/1‑149 (MQ=255)
                                    ctCGTGATCTCAACGATAAGTGGGACGATGCCGACCACTCGCCGAAAAAGGATCAACCGCAGACGTAAGCAGAGTGCTCAACCGTGGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg                                                                                                            <  1:818348/148‑1 (MQ=37)
                                          aTTTCAACGATAAGTGGGACGATGACGCCGAGTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGCGCTCAACCGTAGTCCGGATAAGGCGTTAACGCCGCATCCTGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCtt                                                                                                      <  1:881735/149‑1 (MQ=255)
                                            ttCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt                                                                                                    >  1:287589/1‑149 (MQ=255)
                                             tCAACGATAAGTGGGCCGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   >  1:15416/1‑149 (MQ=255)
                                             tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:540608/149‑1 (MQ=255)
                                             tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:455385/149‑1 (MQ=255)
                                             tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc                                                                                                   <  2:866541/149‑1 (MQ=255)
                                                   aTAAGTGCGACGATGACGACGACTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCa                                                                                             <  1:733778/149‑1 (MQ=255)
                                                    ttaGTGGTACGATGAGGACGACTTGCCGATAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAAACGGAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGAGACGCTCACGCGTCTTATCAgtcaag                                                                                            <  2:264485/147‑6 (MQ=37)
                                                    tAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTACGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg                                                                                            <  2:620018/149‑1 (MQ=255)
                                                         gtgACGATGACGACGCCTGGCCGAAAAAGGAGCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCGCACGCGTCTTATCAGGCCAGGTAAt                                                                                       <  2:1039148/147‑1 (MQ=255)
                                                         gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAAt                                                                                       <  1:101918/149‑1 (MQ=255)
                                                          ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc                                                                                      <  1:267153/149‑1 (MQ=255)
                                                              gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTc                                                                                  >  1:225077/1‑149 (MQ=255)
                                                               ctGACGACGACTGGCCGAAACAGGATCAACCGAAGAAGTCAGCTGAGTGCTCAACCGTAGGCCGGAAAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCATGATGCGACTCTCACGCGTCTTATCAGGCCAGGGAATCTGTCt                                                                                 <  1:755823/148‑1 (MQ=38)
                                                                 tacgaccacTGGCCGTATTAGGTTCAAGCGATGAAGTAAGCTGAGTGCTCAACAGTAGGCCGGATATGGCGTTAACGCCGCAGCCGGCAATGGTGATCGATGCCTGCTGGGACGCTGACGCGTCTTATCAGGCCAGGTATGCTGTCttt                                                                               <  1:231848/148‑1 (MQ=25)
                                                                 gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               >  1:901211/1‑149 (MQ=255)
                                                                 gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               >  1:983041/1‑149 (MQ=255)
                                                                 gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCttt                                                                               <  1:273385/149‑1 (MQ=255)
                                                                  acgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                              <  2:1001535/149‑1 (MQ=255)
                                                                  acgacgacTGGCCGAAAAAGGATAAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                              <  1:360444/149‑1 (MQ=255)
                                                                    gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAc                                                                            >  1:332156/1‑149 (MQ=255)
                                                                     acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca                                                                           >  1:179752/1‑149 (MQ=255)
                                                                      cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat                                                                          <  2:131388/149‑1 (MQ=255)
                                                                       gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCGGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcaaa                                                                         >  2:1026283/1‑147 (MQ=255)
                                                                       gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatc                                                                         >  1:242666/1‑149 (MQ=255)
                                                                               gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGGGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCCGGTATTCTGTCTTTTACATCATTTCAAt                                                                 >  2:806506/1‑149 (MQ=255)
                                                                                aaaaaGGATCAAGCGAAGAAGTAAGCTGATTGCTCAACCGTAGGCCGGATAAGGAGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATc                                                                <  1:569104/149‑1 (MQ=255)
                                                                                 aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATca                                                               >  2:188084/1‑149 (MQ=255)
                                                                                  aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                              <  1:246404/149‑1 (MQ=255)
                                                                                      gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatca                                                          >  1:274173/1‑149 (MQ=255)
                                                                                        tCACCCGACTAAGGAAGCTGAGGGCACCACCGGAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                        <  2:664265/149‑1 (MQ=25)
                                                                                        tCAACCGAAGAAGTAAGCTGAGTGCTCAACCTTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatc                                                        >  2:246888/1‑149 (MQ=255)
                                                                                        tCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCTTTTCAATCAcatcatc                                                        <  1:619711/149‑1 (MQ=255)
                                                                                         cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                                       >  2:1174873/1‑149 (MQ=255)
                                                                                         cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatca                                                       <  1:867141/149‑1 (MQ=255)
                                                                                          aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatcat                                                      >  2:438099/1‑149 (MQ=255)
                                                                                            ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCg                                                    <  2:174560/149‑1 (MQ=255)
                                                                                              gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGcc                                                  <  2:233039/149‑1 (MQ=255)
                                                                                                 taagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAgg                                               <  2:472364/148‑1 (MQ=255)
                                                                                                     tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCCTCGCCAGGCTTa                                           >  2:974302/1‑149 (MQ=255)
                                                                                                           gAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCACCATCGCCAGGCTTACCGCCa                                     >  2:180247/1‑149 (MQ=255)
                                                                                                             gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACt                                   <  2:36250/149‑1 (MQ=255)
                                                                                                                cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATATCAATCACATCCTCATCGCCAGGCTTACCGCCACTTAg                                >  2:564899/1‑149 (MQ=255)
                                                                                                                 tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                               >  1:508811/1‑149 (MQ=255)
                                                                                                                 tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                               >  1:754742/1‑149 (MQ=255)
                                                                                                                  cAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcg                              >  2:1142003/1‑149 (MQ=255)
                                                                                                                   aaCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcgc                             >  1:217070/1‑149 (MQ=255)
                                                                                                                    aCCGTAGGCCGGATAAGGCTTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCt                            <  1:793643/149‑1 (MQ=255)
                                                                                                                     ccGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCCAAGGTGAACGCTGCCTGATGCGACGCTCACGCCTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAAATCATCATCTCCAGGCTTACCGCCACTTTGAGCtt                           >  1:640427/1‑149 (MQ=37)
                                                                                                                      cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCCCATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtc                          >  1:469614/1‑149 (MQ=255)
                                                                                                                      cGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCCGGCTTCCCGCCCCGTAGCGCttg                          >  2:90369/1‑148 (MQ=255)
                                                                                                                           gCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGa                     >  1:1061403/1‑149 (MQ=255)
                                                                                                                            ccGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaa                    >  1:945501/1‑149 (MQ=255)
                                                                                                                             cGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaaa                   >  1:559423/1‑149 (MQ=255)
                                                                                                                             cGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTGTTTTACATCATATCAATCACATAATCATCGCCAGGCTTACCGCAACTTAGCTCTTCCTCGaaa                   >  2:119251/1‑149 (MQ=37)
                                                                                                                                aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataa                <  1:552768/149‑1 (MQ=255)
                                                                                                                                 tatggCGTTAACGCAGCATCCGGCAATGGTGATCGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataat               <  1:339184/146‑1 (MQ=255)
                                                                                                                                     gCGTTAACGCCGCATCCGGCAATGGTGAACGATCCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGCTAATCTGTCTTTTACATCAATTCAATCACATCATAATCGCCACGCTTACCGCCACTTAGCGCTTCGACGAAATAATGttt           >  2:763076/1‑149 (MQ=38)
                                                                                                                                            cgccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATg    >  2:41722/1‑149 (MQ=255)
                                                                                                                                              ccgcATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGt  >  1:474798/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTTTCGGAATGGT  >  NC_000913/2592859‑2593149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: