Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,657,426 G→A 5.8% L504L (CTG→TTG)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,657,4260GA 5.8% 206.2 / 2.5 69L504L (CTG→TTG) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (38/27);  new base A (2/2);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

CATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGC  >  NC_000913/2657285‑2657571
                                                                                                                                             |                                                                                                                                                 
cATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTaa                                                                                                                                            <  2:486269/149‑1 (MQ=255)
    agcagcCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGAGTTCGCTATCGGTCAGACCGTAAGACg                                                                                                                                        >  2:1171385/1‑149 (MQ=255)
        gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                    >  1:835612/1‑149 (MQ=255)
        gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                    <  1:432761/149‑1 (MQ=255)
        gcaagagcgcCGTGCAGACTTTCCATCACACGCTCCGCTTCTACTATTTGTTCTGCCAGCATTCGGGCTTTTACGGCCTGCTCGGCATAGCTCTTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                    <  2:678925/143‑1 (MQ=255)
          cagcGGGCCGTGCAGTCTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTTCCAGCATTCGGGCTTTTACGTCGTGCTCGGCATATCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCTGTCTGACCGTAAGACGGTTTGa                                                                                                                                  <  2:19280/149‑1 (MQ=255)
          cagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGAAAGACGGTTTGa                                                                                                                                  <  2:265148/149‑1 (MQ=255)
          cagcGCGCCGTGCAGACTTTCCACCACACGCGCCGCTTCTACTTTTTGTGCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGa                                                                                                                                  <  1:102129/149‑1 (MQ=255)
           agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                 <  2:164514/149‑1 (MQ=255)
           agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                 <  1:1169989/149‑1 (MQ=255)
           agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAc                                                                                                                                 <  2:44893/149‑1 (MQ=255)
            gcgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAcc                                                                                                                                <  1:865563/149‑1 (MQ=255)
             cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCt                                                                                                                               <  2:979856/149‑1 (MQ=255)
              gcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTg                                                                                                                              >  2:190391/1‑149 (MQ=255)
                gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCTGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCTGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                            <  2:722483/149‑1 (MQ=255)
                gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                            <  1:183907/149‑1 (MQ=255)
                    tGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                                                                                                        >  2:1125738/1‑149 (MQ=255)
                      cAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                            >  1:89509/1‑143 (MQ=255)
                      cAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                            <  2:89509/143‑1 (MQ=255)
                       aGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCGCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGc                                                                                                                     >  2:54265/1‑149 (MQ=255)
                        gACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACTGTTTGACCTGAATAGACGcc                                                                                                                    >  1:706826/1‑149 (MQ=255)
                             tCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTATACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAACTGAATAGACGCCTCAAc                                                                                                               >  1:646753/1‑149 (MQ=255)
                                aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGGTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGAAGGTTTGACCTGAATAGACGCCTCAACGcc                                                                                                            >  1:150270/1‑149 (MQ=255)
                                aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACATGAAGAGACGCCTCAACGcc                                                                                                            >  1:653015/1‑149 (MQ=255)
                                          ccgcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTtc                                                                                                  <  1:579077/149‑1 (MQ=255)
                                               tCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGAACGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCAt                                                                                             >  2:660529/1‑149 (MQ=255)
                                                cTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCaac                                                                                            >  1:1170788/1‑147 (MQ=255)
                                                cTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATc                                                                                            >  2:434584/1‑149 (MQ=255)
                                                 tACTTTTTGTGCTGCAAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCg                                                                                           <  2:974320/149‑1 (MQ=255)
                                                      tttGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTc                                                                                      >  1:1081127/1‑149 (MQ=255)
                                                             tGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGACCTGAATAGCCGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCa                                                                               >  1:661897/1‑149 (MQ=255)
                                                              gcgaGCATTCGGGCTTTTAGGTGCTGCTCGGCATATCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATGGGTCAGACAGTAAGACGGTTTGTCCTGAATAGACGCCTCATCTCCGGTGGATTTCTCCATCGCCGTCACGCTCaa                                                                              <  2:171329/146‑1 (MQ=255)
                                                                cAGCATTCTGGCTTTTACGTACTGCTCGGCATAGCTCATTGAGTCATTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCGCAACGCCGGTGGAGTTCTCCAGCGCCGTCACGCTCaaaa                                                                            <  1:702513/149‑1 (MQ=255)
                                                                cAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCaaaa                                                                            >  1:466550/1‑149 (MQ=255)
                                                                 aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                           <  2:1156729/149‑1 (MQ=255)
                                                                    aTTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGAcc                                                                        >  2:359497/1‑149 (MQ=255)
                                                                     ttCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCg                                                                       >  1:630580/1‑149 (MQ=255)
                                                                           cTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATAATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCa                                                                 <  1:866837/149‑1 (MQ=255)
                                                                                aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAc                                                            >  1:834772/1‑149 (MQ=255)
                                                                                aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGACGTCACGCTCAAAAGACCGTCGGCATCGAc                                                            >  2:488494/1‑149 (MQ=255)
                                                                                aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGATTGACCTGAATAGACGACTCAACGCCGGTCGATTTCTCCATCGCCGTCACGCTCAAAAGACCGACGGCATCGAc                                                            >  2:1126195/1‑149 (MQ=255)
                                                                                   tCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGATTAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACTCTCAAAAGACCGTCGGCATCGACCTg                                                         >  1:124210/1‑149 (MQ=255)
                                                                                   tCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGACACGCTCAAAAGACCGTCGGCATCGACCTg                                                         >  1:124209/1‑149 (MQ=255)
                                                                                    ccTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTACTCCATCGCCGTCACGCTCTAAAGACCGTCGGCATCGCACTgg                                                        >  2:419711/1‑149 (MQ=255)
                                                                                      tGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGaa                                                      >  2:721588/1‑149 (MQ=255)
                                                                                       gCTCGGCGTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACATGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAACGACCGTCGGCATCGCCCTGGCAc                                                     >  2:701987/1‑149 (MQ=255)
                                                                                           ggCATAGCGCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCa                                                 <  2:455510/149‑1 (MQ=255)
                                                                                              aTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGTTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcgc                                              <  2:838984/149‑1 (MQ=255)
                                                                                              aTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcgc                                              <  2:834667/149‑1 (MQ=255)
                                                                                                 tcTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGaa                                           <  1:611635/148‑1 (MQ=255)
                                                                                                 gCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCGCAAAAGCCCGTCGGCATCGACCTGGAACGTCACGCGat                                           >  2:306983/1‑148 (MQ=255)
                                                                                                    cATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTTAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGGATCGACCTGGAACGTCACGCGAatat                                        >  1:65820/1‑149 (MQ=255)
                                                                                                     aTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGCACGTCCCGCGAATATg                                       >  2:392419/1‑149 (MQ=255)
                                                                                                      ttGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATgc                                      >  2:464742/1‑149 (MQ=255)
                                                                                                       agAGTCTTAGATCATCGAAGCTATTTCGCTATCGGGCAGACCGTAAGACGGGTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCCTCGCCGTCACGCTCAAAAGACCGGCGGCATCGACCTGGAACGTCACGCGAATATgcg                                     <  2:143120/148‑1 (MQ=255)
                                                                                                         aGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCa                                   >  2:637261/1‑149 (MQ=255)
                                                                                                                 atcatcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGc                           >  1:958727/1‑149 (MQ=255)
                                                                                                                   catcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAATGTCCCGCGAATATGCGCACCGCCAGCCg                         >  2:690105/1‑149 (MQ=255)
                                                                                                                    atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                        <  2:924383/149‑1 (MQ=255)
                                                                                                                    atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCAAGCCgg                        >  1:1023996/1‑149 (MQ=255)
                                                                                                                    atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGAGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                        >  1:144996/1‑149 (MQ=255)
                                                                                                                         aGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGATTAGACGCATCAACGCCGGTGGATTTATCCATCGCCGTCCCGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAgcc                   >  1:769673/1‑148 (MQ=255)
                                                                                                                                      tCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACg      <  1:451750/149‑1 (MQ=255)
                                                                                                                                        ggTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa    <  1:190391/149‑1 (MQ=255)
                                                                                                                                        ggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa    >  1:759921/1‑149 (MQ=255)
                                                                                                                                         ggccgACCGTACGACGGTTTGACCTGAATCGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAg   <  2:653015/145‑1 (MQ=255)
                                                                                                                                         gTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGCCCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGccg   >  2:623021/1‑147 (MQ=255)
                                                                                                                                          tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAgc  >  1:738551/1‑149 (MQ=255)
                                                                                                                                          tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAgc  <  1:1125738/149‑1 (MQ=255)
                                                                                                                                          tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGAATCGACCTGGAAAGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCCCGCAgc  >  2:580840/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGC  >  NC_000913/2657285‑2657571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑