Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,657,440 T→C 5.8% K499R (AAA→AGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,657,4400TC 5.8% 200.2 / 3.1 69K499R (AAA→AGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base T (39/26);  new base C (2/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

GCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCA  >  NC_000913/2657293‑2657584
                                                                                                                                                   |                                                                                                                                                
gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                 >  1:835612/1‑149 (MQ=255)
gccagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                 <  1:432761/149‑1 (MQ=255)
gcaagagcgcCGTGCAGACTTTCCATCACACGCTCCGCTTCTACTATTTGTTCTGCCAGCATTCGGGCTTTTACGGCCTGCTCGGCATAGCTCTTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGttt                                                                                                                                                 <  2:678925/143‑1 (MQ=255)
  cagcGGGCCGTGCAGTCTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTTCCAGCATTCGGGCTTTTACGTCGTGCTCGGCATATCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCTGTCTGACCGTAAGACGGTTTGa                                                                                                                                               <  2:19280/149‑1 (MQ=255)
  cagcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGAAAGACGGTTTGa                                                                                                                                               <  2:265148/149‑1 (MQ=255)
  cagcGCGCCGTGCAGACTTTCCACCACACGCGCCGCTTCTACTTTTTGTGCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGa                                                                                                                                               <  1:102129/149‑1 (MQ=255)
   agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                              <  1:1169989/149‑1 (MQ=255)
   agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAc                                                                                                                                              <  2:164514/149‑1 (MQ=255)
   agcGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAc                                                                                                                                              <  2:44893/149‑1 (MQ=255)
    gcgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGAcc                                                                                                                                             <  1:865563/149‑1 (MQ=255)
     cgcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCt                                                                                                                                            <  2:979856/149‑1 (MQ=255)
      gcgcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTg                                                                                                                                           >  2:190391/1‑149 (MQ=255)
        gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCTGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCTGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                                         <  2:722483/149‑1 (MQ=255)
        gcCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                                         <  1:183907/149‑1 (MQ=255)
            tGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGa                                                                                                                                     >  2:1125738/1‑149 (MQ=255)
              cAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                                         >  1:89509/1‑143 (MQ=255)
              cAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGaa                                                                                                                                         <  2:89509/143‑1 (MQ=255)
               aGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCGCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGc                                                                                                                                  >  2:54265/1‑149 (MQ=255)
                gACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACTGTTTGACCTGAATAGACGcc                                                                                                                                 >  1:706826/1‑149 (MQ=255)
                     tCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTATACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGAACTGAATAGACGCCTCAAc                                                                                                                            >  1:646753/1‑149 (MQ=255)
                        aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGGTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGAAGGTTTGACCTGAATAGACGCCTCAACGcc                                                                                                                         >  1:150270/1‑149 (MQ=255)
                        aGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACATGAAGAGACGCCTCAACGcc                                                                                                                         >  1:653015/1‑149 (MQ=255)
                                  ccgcTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTtc                                                                                                               <  1:579077/149‑1 (MQ=255)
                                       tCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGAACGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCAt                                                                                                          >  2:660529/1‑149 (MQ=255)
                                        cTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCaac                                                                                                         >  1:1170788/1‑147 (MQ=255)
                                        cTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATc                                                                                                         >  2:434584/1‑149 (MQ=255)
                                         tACTTTTTGTGCTGCAAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCg                                                                                                        <  2:974320/149‑1 (MQ=255)
                                              tttGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTc                                                                                                   >  1:1081127/1‑149 (MQ=255)
                                                     tGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAAACCGTCTTACGGTCTGACCTGAATAGCCGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCa                                                                                            >  1:661897/1‑149 (MQ=255)
                                                      gcgaGCATTCGGGCTTTTAGGTGCTGCTCGGCATATCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATGGGTCAGACAGTAAGACGGTTTGTCCTGAATAGACGCCTCATCTCCGGTGGATTTCTCCATCGCCGTCACGCTCaa                                                                                           <  2:171329/146‑1 (MQ=255)
                                                        cAGCATTCTGGCTTTTACGTACTGCTCGGCATAGCTCATTGAGTCATTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCGCAACGCCGGTGGAGTTCTCCAGCGCCGTCACGCTCaaaa                                                                                         <  1:702513/149‑1 (MQ=255)
                                                        cAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCaaaa                                                                                         >  1:466550/1‑149 (MQ=255)
                                                         aGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAg                                                                                        <  2:1156729/149‑1 (MQ=255)
                                                            aTTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGAcc                                                                                     >  2:359497/1‑149 (MQ=255)
                                                             ttCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCg                                                                                    >  1:630580/1‑149 (MQ=255)
                                                                   cTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATAATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCa                                                                              <  1:866837/149‑1 (MQ=255)
                                                                        aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGAc                                                                         >  1:834772/1‑149 (MQ=255)
                                                                        aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGACGTCACGCTCAAAAGACCGTCGGCATCGAc                                                                         >  2:488494/1‑149 (MQ=255)
                                                                        aCGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGATTGACCTGAATAGACGACTCAACGCCGGTCGATTTCTCCATCGCCGTCACGCTCAAAAGACCGACGGCATCGAc                                                                         >  2:1126195/1‑149 (MQ=255)
                                                                           tCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGATTAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACTCTCAAAAGACCGTCGGCATCGACCTg                                                                      >  1:124210/1‑149 (MQ=255)
                                                                           tCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGACACGCTCAAAAGACCGTCGGCATCGACCTg                                                                      >  1:124209/1‑149 (MQ=255)
                                                                            ccTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTACTCCATCGCCGTCACGCTCTAAAGACCGTCGGCATCGCACTgg                                                                     >  2:419711/1‑149 (MQ=255)
                                                                              tGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGaa                                                                   >  2:721588/1‑149 (MQ=255)
                                                                               gCTCGGCGTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACATGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAACGACCGTCGGCATCGCCCTGGCAc                                                                  >  2:701987/1‑149 (MQ=255)
                                                                                   ggCATAGCGCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCa                                                              <  2:455510/149‑1 (MQ=255)
                                                                                      aTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGTTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcgc                                                           <  2:838984/149‑1 (MQ=255)
                                                                                      aTAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCAcgc                                                           <  2:834667/149‑1 (MQ=255)
                                                                                         tcTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGaa                                                        <  1:611635/148‑1 (MQ=255)
                                                                                         gCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCGCAAAAGCCCGTCGGCATCGACCTGGAACGTCACGCGat                                                        >  2:306983/1‑148 (MQ=255)
                                                                                            cATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTTAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGGATCGACCTGGAACGTCACGCGAatat                                                     >  1:65820/1‑149 (MQ=255)
                                                                                             aTTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGCACGTCCCGCGAATATg                                                    >  2:392419/1‑149 (MQ=255)
                                                                                              ttGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATgc                                                   >  2:464742/1‑149 (MQ=255)
                                                                                               agAGTCTTAGATCATCGAAGCTATTTCGCTATCGGGCAGACCGTAAGACGGGTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCCTCGCCGTCACGCTCAAAAGACCGGCGGCATCGACCTGGAACGTCACGCGAATATgcg                                                  <  2:143120/148‑1 (MQ=255)
                                                                                                 aGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCa                                                >  2:637261/1‑149 (MQ=255)
                                                                                                         atcatcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGc                                        >  1:958727/1‑149 (MQ=255)
                                                                                                           catcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAATGTCCCGCGAATATGCGCACCGCCAGCCg                                      >  2:690105/1‑149 (MQ=255)
                                                                                                            atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                     <  2:924383/149‑1 (MQ=255)
                                                                                                            atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCAAGCCgg                                     >  1:1023996/1‑149 (MQ=255)
                                                                                                            atcGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGAGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCgg                                     >  1:144996/1‑149 (MQ=255)
                                                                                                                 aGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGATTAGACGCATCAACGCCGGTGGATTTATCCATCGCCGTCCCGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAgcc                                >  1:769673/1‑148 (MQ=255)
                                                                                                                              tCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACg                   <  1:451750/149‑1 (MQ=255)
                                                                                                                                ggTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa                 <  1:190391/149‑1 (MQ=255)
                                                                                                                                ggTCAAACCGTCTTACGGTCTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCa                 >  1:759921/1‑149 (MQ=255)
                                                                                                                                 ggccgACCGTACGACGGTTTGACCTGAATCGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAg                <  2:653015/145‑1 (MQ=255)
                                                                                                                                 gTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGCCCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGccg                >  2:623021/1‑147 (MQ=255)
                                                                                                                                  tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAgc               >  1:738551/1‑149 (MQ=255)
                                                                                                                                  tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAgc               <  1:1125738/149‑1 (MQ=255)
                                                                                                                                  tCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGAATCGACCTGGAAAGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCCCGCAgc               >  2:580840/1‑149 (MQ=255)
                                                                                                                                      aCCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCaa           >  1:282362/1‑149 (MQ=255)
                                                                                                                                        cGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCCCGCAGCGCaaac         >  1:413827/1‑148 (MQ=255)
                                                                                                                                              aCGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGCCCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAACCGCGcc   >  1:1063148/1‑149 (MQ=255)
                                                                                                                                               cGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCa  <  1:660529/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTGCCAGCATTCGGGCTTTTACGTCCTGCTCGGCATAGCTCATTGAGTCTTTGATCATCGAAGCGATTTCGCTATCGGTCAGACCGTAAGACGGTTTGACCTGAATAGACGCCTCAACGCCGGTGGATTTCTCCATCGCCGTCACGCTCAAAAGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCA  >  NC_000913/2657293‑2657584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑