Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,761,623 C→A 6.7% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,761,6230CA 6.7% 196.2 / 2.8 77V640L (GTA→TTA) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base C (37/34);  new base A (3/3);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

AAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCA  >  NC_000913/2761476‑2761765
                                                                                                                                                   |                                                                                                                                              
aaaaTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa                                                                                                                                               <  1:730763/149‑1 (MQ=255)
aaaaTTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACa                                                                                                                                               >  1:334563/1‑149 (MQ=255)
     tCCAGCAAGTCACCCAGTCGGTGTAACGTCTGAATGGTAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtg                                                                                                                                          <  1:694997/149‑1 (MQ=255)
         gCAAGTCTACCAGTCGGTGTAACGACTGAATTGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGAAGGAAACTATCGAACGCCAATTTGACATGAGTGTAATCGACTTAGCTGCGTATACCACGCTTAACAGCGTGgtgt                                                                                                                                      >  1:724054/1‑149 (MQ=255)
          cAAGTCTCCCCGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGtt                                                                                                                                     <  2:265819/149‑1 (MQ=255)
          cAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATATTTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGGGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGtt                                                                                                                                     <  1:1057094/149‑1 (MQ=255)
           aaGTCTACAAGTCGGTGTAACTTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGCACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttt                                                                                                                                    <  1:242319/149‑1 (MQ=255)
            aGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatac                                                                                                                                    <  2:575418/148‑5 (MQ=255)
            aGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgtggtatac                                                                                                                                    >  1:575418/1‑144 (MQ=255)
              tCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACTCTTTACAGCGTGGTGTTTAAc                                                                                                                                 >  2:959001/1‑149 (MQ=255)
                tACCAGTCGGTGTAACGTCTGAATGGGAATTCCAATCTCTTATAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATAGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttttccta                                                                                                                               >  2:752659/1‑144 (MQ=255)
                 aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTTACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTAc                                                                                                                              >  2:1099543/1‑149 (MQ=255)
                 aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTAc                                                                                                                              >  2:1099542/1‑149 (MQ=255)
                 aCCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGCTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGATTAACTAc                                                                                                                              >  1:1125118/1‑149 (MQ=255)
                  ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACa                                                                                                                             >  2:1009442/1‑149 (MQ=255)
                  ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACa                                                                                                                             >  1:324965/1‑149 (MQ=255)
                  ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAAAGCGTGGTGTTTAACTACa                                                                                                                             >  2:1177384/1‑149 (MQ=255)
                        ggTGTAACGTCTGAATGGGAACGCCAATCTCTTCTAAGGCATTGACACCTGGGGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACAtcctcc                                                                                                                       <  1:973831/149‑1 (MQ=255)
                          tgtAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCAt                                                                                                                     >  2:597383/1‑149 (MQ=255)
                                  cTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGGAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa                                                                                                             <  2:273854/149‑1 (MQ=255)
                                   tGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAg                                                                                                            <  2:1175092/149‑1 (MQ=255)
                                    gAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGc                                                                                                           <  2:18868/149‑1 (MQ=255)
                                    ccaTGTGAATGCCAATCTCTTCACAGGCATTGACTCCAGCTGTATGATGGAAACTTTCTAACGCCAATTTGACATGTGTGTAATCGACTTTGATGCGAATCCCCCGCTTTACAGCGTGGTGTTAAACTACATCCTCCATCAAGCTCAGc                                                                                                           <  2:809691/147‑1 (MQ=255)
                                     aaTGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCa                                                                                                          <  1:1009442/149‑1 (MQ=255)
                                          gAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                     >  1:804904/1‑149 (MQ=255)
                                              gCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                                 >  1:763088/1‑149 (MQ=255)
                                                cAATCTCTTCTAAGGCATTGACACCTGGTGGAAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                               >  1:1085805/1‑149 (MQ=255)
                                                     tcttctAAGGCATTTACACCTGGTTGTAGATGGAAACTTTCGAACTCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGtata                                                                                          <  2:198717/149‑1 (MQ=255)
                                                      cttctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAg                                                                                         >  1:445184/1‑149 (MQ=255)
                                                        tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTTTAGcc                                                                                       >  1:1072522/1‑149 (MQ=255)
                                                         ctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCg                                                                                      >  2:1115058/1‑149 (MQ=255)
                                                           aaGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                                                                    >  2:943846/1‑149 (MQ=255)
                                                           aaGGCATTGACACCGGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                                                                    <  2:661709/149‑1 (MQ=255)
                                                            aGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg                                                                                   <  1:433323/149‑1 (MQ=255)
                                                              gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGt                                                                                 >  1:27309/1‑149 (MQ=255)
                                                              gCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGt                                                                                 >  1:1173086/1‑149 (MQ=255)
                                                                  tGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCCTGGTGTTTAACTACATCCTCCATCAAGCTGAGAACCTTAGGTAGGTTATAGCCGAAGGTATTg                                                                             >  1:81966/1‑149 (MQ=255)
                                                                   gACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTgg                                                                            <  2:946990/149‑1 (MQ=255)
                                                                     cacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATTTGTGTAATCGACTTTGCTGCGTCTACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGtt                                                                          <  1:115462/149‑1 (MQ=255)
                                                                       ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTTGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt                                                                        >  1:1086569/1‑149 (MQ=255)
                                                                       ccTGGTGGGAGATGGAAACTTTCGAACGCCAATTTGACCTGTGTGAAATCGACTTTGCTGCGTAGACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTAt                                                                        <  1:799145/149‑1 (MQ=255)
                                                                        cTGGTGGTAGATGGAAACTTTCGACCGCCAATTTGACTTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTAAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATg                                                                       <  1:872721/149‑1 (MQ=255)
                                                                           gtggcAGATCGACTCTATCGAACGCCAAGATGACATCTGAGTAATCGACTTTGCGGCGTAATCCACGCGGTACAGCGTGGTGTTTAACTACAGCCTCCAGCAAGCTGAGCACCGTCGGGAGGGTATAGCCGAAGGTAGTGGTTATGAGc                                                                    <  2:779674/149‑1 (MQ=255)
                                                                            tggtAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACGTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCt                                                                   <  2:385830/149‑1 (MQ=255)
                                                                                 taTGTAAACATTCGAACGGCAATTTGACCTGTGTGTAATCGTCTTTGCTGCGTATAACACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATCGCCGAAGGTATTGGTTATGAGCTTCaaa                                                              <  2:652771/148‑1 (MQ=255)
                                                                                    ggAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCa                                                           >  2:379715/1‑149 (MQ=255)
                                                                                    ggAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCa                                                           >  2:274635/1‑149 (MQ=255)
                                                                                         cTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgtt                                                      <  2:396302/149‑1 (MQ=255)
                                                                                          tttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgttg                                                     >  1:1090954/1‑149 (MQ=255)
                                                                                          tttCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgttg                                                     >  1:875982/1‑149 (MQ=255)
                                                                                                  gCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATATCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAAt                                             >  1:1162960/1‑149 (MQ=255)
                                                                                                     aaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGa                                          >  1:565068/1‑149 (MQ=255)
                                                                                                       tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaaa                                        <  2:445869/149‑1 (MQ=255)
                                                                                                             aTGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCCAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGCTCTTCAAATCATTTTTGAGGGCAATGGAAAGAGAAc                                  >  2:1068564/1‑149 (MQ=255)
                                                                                                              tgtgtgTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGTCAATGGAAAGAGTACg                                 >  1:265074/1‑149 (MQ=255)
                                                                                                               gtgtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGt                                >  2:67319/1‑149 (MQ=255)
                                                                                                                  tgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtc                             >  1:294355/1‑149 (MQ=255)
                                                                                                                  tgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtc                             <  2:1085805/149‑1 (MQ=255)
                                                                                                                   gtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtct                            <  2:804904/149‑1 (MQ=255)
                                                                                                                    tAATCGCCTTTGCTGCGAATACCACGCTTTACAGCGTGGTGTTAAACTACATCCTCCATCAAGCTGAGCAACTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTtctc                           <  2:819053/149‑1 (MQ=255)
                                                                                                                     aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcc                          <  2:261543/149‑1 (MQ=255)
                                                                                                                      aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTccc                         <  2:763088/149‑1 (MQ=255)
                                                                                                                         taCTTTGCTGCTTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCtt                      <  2:164736/148‑1 (MQ=255)
                                                                                                                           cTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                    >  2:466502/1‑149 (MQ=255)
                                                                                                                           cTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                    <  2:1173086/149‑1 (MQ=255)
                                                                                                                           cTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                    <  2:27309/149‑1 (MQ=255)
                                                                                                                           atttGCTGCGTATACCACGCTTTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCa                    <  1:1107506/148‑1 (MQ=255)
                                                                                                                            tttGCTGCGTATACCACGCTTTACAGCGTGGTGTTAAACAACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAg                   <  1:1184384/149‑1 (MQ=255)
                                                                                                                                 tgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc              <  1:1136382/149‑1 (MQ=255)
                                                                                                                                 tgcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATc              >  1:396850/1‑149 (MQ=255)
                                                                                                                                  gcGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCAGAGCACCTTAGGTAGTGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGTGCAATGGAAAGAGTACTATTCTCCCCTTCAGAAAtac             >  2:680392/1‑147 (MQ=255)
                                                                                                                                    aaacaCCACGCTGTAAAGCGTGGTATTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCAc           >  2:211031/5‑149 (MQ=255)
                                                                                                                                     tataCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACt          >  1:1135636/1‑149 (MQ=255)
                                                                                                                                         ccACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTTTGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCg      >  2:1155073/1‑149 (MQ=255)
                                                                                                                                          cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt     <  1:1177384/149‑1 (MQ=255)
                                                                                                                                          cACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGt     >  1:494778/1‑149 (MQ=255)
                                                                                                                                             gCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCa  <  1:943846/149‑1 (MQ=255)
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AAAATTCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCGTGCA  >  NC_000913/2761476‑2761765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: