Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,503 C→T 5.7% intergenic (+482/‑42) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,5030CT 5.7% 300.8 / 6.6 105intergenic (+482/‑42)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (46/53);  new base T (2/4);  total (48/57)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

Only 100 of 109 total aligned reads displayed.

TGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAT  >  NC_000913/2775355‑2775648
                                                                                                                                                    |                                                                                                                                                 
tGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAtctc                                                                                                                                                   >  2:1006689/1‑149 (MQ=255)
  tAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTATGTCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                 >  2:197313/1‑149 (MQ=255)
   aaaTGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTCGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAgg                                                                                                                                                <  1:609917/149‑1 (MQ=255)
       gCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTc                                                                                                                                            >  1:108222/1‑149 (MQ=255)
        ccccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTcg                                                                                                                                           >  2:1161266/1‑148 (MQ=255)
        cacatACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCAATTCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCt                                                                                                                                           <  2:1060970/145‑1 (MQ=255)
          ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCTGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCATGATTCTGc                                                                                                                                         >  1:1045621/1‑149 (MQ=255)
          ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGc                                                                                                                                         <  1:524538/149‑1 (MQ=255)
           cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGcc                                                                                                                                        <  2:870780/149‑1 (MQ=255)
           cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGcc                                                                                                                                        <  1:203350/149‑1 (MQ=255)
            tACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCAATCAGATATATATCATTTCTACAAACATACCCCATTAACGGCAGAATCTCAGGATTCTGCCg                                                                                                                                       >  1:216083/1‑149 (MQ=255)
             aCAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGAATAACGGCAGAATCTCAGGATTCTGCCGc                                                                                                                                      >  2:614512/1‑149 (MQ=255)
              cAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCt                                                                                                                                     >  2:245238/1‑149 (MQ=255)
               aaaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgtta                                                                                                                                    <  2:99073/149‑4 (MQ=255)
                 aaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttt                                                                                                                                  >  2:57338/1‑149 (MQ=255)
                  aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTGCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 <  2:666205/149‑1 (MQ=255)
                  aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 >  2:302473/1‑149 (MQ=255)
                  aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttttt                                                                                                                                 <  2:385122/149‑1 (MQ=255)
                     aaCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttat                                                                                                                              >  2:171128/1‑149 (MQ=255)
                      aCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttatt                                                                                                                             <  2:897258/149‑1 (MQ=255)
                         attattTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTattatt                                                                                                                          >  2:30864/1‑149 (MQ=255)
                          ttattTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTAttt                                                                                                                         >  2:104515/1‑149 (MQ=255)
                               tttAGATGTCGGCTGGAACTCCATCTTCCTCTTTTGGCACAGAACTGCCTTTCATCAATATAAAAGCATTCAGATATATATGATTTCTACAAACATACCCGATTAACGGCAGCATCGCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                    <  1:1075053/149‑1 (MQ=255)
                               tttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATATAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                    <  2:429845/149‑1 (MQ=255)
                                ttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAgg                                                                                                                   <  2:52587/149‑1 (MQ=255)
                                 tAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAggg                                                                                                                  <  2:354470/149‑1 (MQ=255)
                                 tAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAggg                                                                                                                  >  1:447564/1‑149 (MQ=255)
                                    agGGCGTCTGGCAATCCAACTTCCGCTTTTTGCACAGAACGGCCTATCAGCAATATATAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGag                                                                                                               <  1:660047/149‑1 (MQ=255)
                                         gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAgtagta                                                                                                          >  2:350159/1‑149 (MQ=255)
                                         gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAgtagta                                                                                                          >  2:15622/1‑149 (MQ=255)
                                            tGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGGATTCAGATATATATCATTTCTACAAACATCCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATg                                                                                                       <  1:675374/149‑1 (MQ=255)
                                            tGGCACTCCAACTTCCGCTTTTGGCAAATAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGTAGTAGTAATg                                                                                                       <  2:991724/149‑1 (MQ=255)
                                            tGGCACTCCAACTTCCGCTTTTCGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCGTTTTTATTATTTTTCAGGGGAGTAGTAATg                                                                                                       >  1:768173/1‑149 (MQ=255)
                                             ggCACTCCAACATCCGCTTTAGGCACAGAACGGCCTAGCAGCAAGATAAAAGCATTCAGATATATATCATTACTACAAACATACCCGATTAACGGCAGAAACTCAGCATTATGCCGCTTTTTTATTTTTTTTCAGGGGAGTAGAAATgc                                                                                                      >  2:816371/1‑148 (MQ=255)
                                              gCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAg                                                                                                     >  2:721983/1‑149 (MQ=255)
                                                 cTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCaa                                                                                                  <  2:958605/149‑1 (MQ=255)
                                                   ccAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAct                                                                                                <  2:43362/149‑1 (MQ=255)
                                                    cAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAActc                                                                                               >  1:1115318/1‑149 (MQ=255)
                                                     aaCTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATAAACGGCAGAATCTCAGGATTCTGCCGATTTTTAATAAATTTTCAGGGGAGTAGTAATGAGCAActct                                                                                              >  2:1158988/1‑149 (MQ=255)
                                                        ttCCGCTTTTGGCACAGACAGGCCTATCAGCAAAATAAAAGCATTCAGATATATATCATTTCTACACACATACCCGATAAACGGCAGAATCTCAGGATTCTTCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATTAGCAACTCTGaa                                                                                           <  2:1003551/149‑1 (MQ=255)
                                                         tCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                          >  1:159425/1‑149 (MQ=255)
                                                         tCCGCTTTTGGCAAAGAACGGCCTATCATCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                          <  1:526702/149‑1 (MQ=255)
                                                           cGCTTTTGGCAGAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                        <  2:862869/149‑1 (MQ=255)
                                                           cGCTTTTGGCACAGAACGGCCGATCAGCAATATAAAAGCATTCAGATATATATCCTTTCTACAAACATACCCGATTAACGGCAGAATCTCAGTATTCTGCCGGTTTTTTATTATTTTTAAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                        >  1:744236/1‑149 (MQ=255)
                                                             cTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTg                                                                                      <  2:560886/149‑1 (MQ=255)
                                                                ttGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTa                                                                                   <  1:291717/149‑1 (MQ=255)
                                                                    cacaGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCa                                                                               >  1:1083426/1‑149 (MQ=255)
                                                                      caGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtt                                                                             >  1:260902/1‑149 (MQ=255)
                                                                       aGAACGGCCTATCAGCAATATAAAAGCATTCAGTTATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAttt                                                                            <  1:322255/149‑1 (MQ=255)
                                                                       aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTTTTATTTTTCAGGGGAGTAGTAATGAGAAACTCTGAAGGTTTGTAGTCAttt                                                                            >  1:1181802/1‑149 (MQ=255)
                                                                         aatcgacTATCAGAAATATAAAATCATTAAGATATAAATAATTTCTACAAACATACCAGATTAACGGCAGAATCTCATGATTAGGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGGCATTTTa                                                                          <  1:812094/143‑1 (MQ=255)
                                                                         aaCGGCCTATCAGCAATATAAAAGCATTCTGATATATAGCATTTCTACAAACATACCCGATTAACGGCAGAAGCTCCGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                          <  1:802364/149‑1 (MQ=255)
                                                                         aaCGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                          >  2:77139/1‑149 (MQ=255)
                                                                          aCGGCCTATCAGCTATATAAAAGCATTCAGATATATTTCATTTTTACTAACACACCGGATTAACGGCATAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCATGGGAGTAGTAATGAGCAACTCTGAAGGTTGTTAGTCATTTTAg                                                                         <  2:493855/149‑1 (MQ=255)
                                                                           cGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTATGCCGCTTTTTTATTATTTTTAAGGGGAGTAGTAATGATCAACTCTGAATGTTTGTAGTCATTTTata                                                                        >  1:311965/1‑147 (MQ=255)
                                                                                 aTCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCGTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCACCTCTGAAGGGTGGTAGGCATTTTTGCACACGc                                                                  >  2:656483/1‑149 (MQ=255)
                                                                                  tCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTTAAGGTTGGTAGTCATTTTAGCAAACGCt                                                                 >  2:493085/1‑149 (MQ=255)
                                                                                  tCAGCAATATAAAAGCATTAAGATATATATCATTTCTACAAACATACCCGATTATCTTCAGAATCTCAGGATTCTGCCTCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCt                                                                 <  2:859818/149‑1 (MQ=255)
                                                                                       aaTATTAAAGCATTCAGTTATATATCATTTCTACATACATTCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:370321/149‑1 (MQ=255)
                                                                                       aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTTTTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:350159/149‑1 (MQ=255)
                                                                                       aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  2:163827/149‑1 (MQ=255)
                                                                                       aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCGGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  2:108222/149‑1 (MQ=255)
                                                                                       aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATGCTGCCGCTTTTTTATTATTTATCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                            <  1:1032026/149‑1 (MQ=255)
                                                                                            aaaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTg                                                       >  1:619083/1‑149 (MQ=255)
                                                                                              aaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGcc                                                     <  1:972167/149‑1 (MQ=255)
                                                                                                  aTTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAg                                                 >  2:583744/1‑149 (MQ=255)
                                                                                                    tCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTg                                               <  2:159425/149‑1 (MQ=255)
                                                                                                    tCAGATATATATAATATCTACACCCCTCCCGTATTAACGGCACACTCTCTGGATTCGGCCGCTTTCTTATTATTTTTCAGCCGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTCGCAAACGCTTTCTGGTCTGCCGCAGTg                                               <  2:302005/149‑1 (MQ=255)
                                                                                                     atgATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTgg                                              <  2:1125074/147‑1 (MQ=255)
                                                                                                      aGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGATCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTggg                                             >  1:830460/1‑149 (MQ=255)
                                                                                                      aGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTggg                                             >  1:429385/1‑149 (MQ=255)
                                                                                                       gATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAAAGCTTTCTGGTCTGCCGCAGTGGGc                                            >  1:188633/1‑149 (MQ=255)
                                                                                                         tatatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGGCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCAt                                          <  2:447564/149‑1 (MQ=255)
                                                                                                          atatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATc                                         <  1:614512/149‑1 (MQ=255)
                                                                                                           tatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATAATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTATCTGGTCTGCCGCAGTGGGCATCg                                        >  2:882088/1‑149 (MQ=255)
                                                                                                            atatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCgg                                       <  2:445265/149‑1 (MQ=255)
                                                                                                             tatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATAATATTACAGGGGAGTAGTAATAAGCAACAGTCAAGTTTGGTAGTCATTTTATCAAACGCTATCTGGTCTGCCGCAGAGGCCATCgcc                                      >  2:1182128/1‑147 (MQ=255)
                                                                                                              atCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                     >  2:608052/1‑149 (MQ=255)
                                                                                                               tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTg                                    >  1:521681/1‑149 (MQ=255)
                                                                                                               tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTg                                    >  2:931093/1‑149 (MQ=255)
                                                                                                                      tACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGt                             <  1:104515/149‑1 (MQ=255)
                                                                                                                       aCAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTa                            >  1:1111546/1‑149 (MQ=255)
                                                                                                                        cAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                           >  1:233011/1‑149 (MQ=255)
                                                                                                                        cAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                           >  2:1102693/1‑149 (MQ=255)
                                                                                                                         aaaCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGc                          <  1:245238/149‑1 (MQ=255)
                                                                                                                           aCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAg                        >  1:250576/1‑149 (MQ=255)
                                                                                                                           aCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGACTGCGTAGCAg                        >  1:573564/1‑149 (MQ=255)
                                                                                                                            cATACCCGATTCACGGCAGACTCTCAGGATTCTGCCGCTTTTTTATTATTTTTCCGGGGAGTAGTAATGAGCAACTCTGACGGGTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAgg                       <  1:656483/149‑1 (MQ=255)
                                                                                                                             aTACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAggg                      <  2:472431/149‑1 (MQ=255)
                                                                                                                               aCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGcc                    <  1:26215/149‑1 (MQ=255)
                                                                                                                                 ccGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                  >  2:580108/1‑149 (MQ=255)
                                                                                                                                   gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTACTGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                <  1:1161266/149‑1 (MQ=255)
                                                                                                                                   gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                <  2:849150/149‑1 (MQ=255)
                                                                                                                                   aaaaagcGGCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTg                >  2:1103415/7‑149 (MQ=255)
                                                                                                                                          ggCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTCGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGACATTGGTATCTg         >  1:719819/1‑149 (MQ=255)
                                                                                                                                             aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTGCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCg      <  1:171128/149‑1 (MQ=255)
                                                                                                                                              gAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTGTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg     <  2:361942/149‑1 (MQ=255)
                                                                                                                                                 tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  <  1:404380/149‑1 (MQ=255)
                                                                                                                                                 tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  >  2:404057/1‑149 (MQ=255)
                                                                                                                                                 tctcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGGGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAt  <  2:233011/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTAT  >  NC_000913/2775355‑2775648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: