Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,140 A→G 6.6% intergenic (+152/+38) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1400AG 6.6% 249.4 / 7.2 91intergenic (+152/+38)sibC/serAsRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (34/50);  new base G (3/3);  total (37/54)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GTTACAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTG  >  NC_000913/3056992‑3057288
                                                                                                                                                     |                                                                                                                                                    
tttACAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGCCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCTGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCaa                                                                                                                                                      <  2:797231/148‑1 (MQ=255)
gTTACAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCaa                                                                                                                                                      <  2:495364/149‑1 (MQ=255)
 ttACAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAg                                                                                                                                                     >  1:1087845/1‑149 (MQ=255)
      gCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGt                                                                                                                                                <  2:837169/149‑1 (MQ=255)
        cccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                              >  2:55316/1‑149 (MQ=255)
         ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAATGGATAAAAAAACGGGCAAGTCAGTGAc                                                                                                                                             <  1:544769/149‑1 (MQ=255)
         ccATGCTGCCCTGCCATCGTAAAACCCCATAAAATAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGCAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAc                                                                                                                                             <  2:904336/149‑1 (MQ=255)
               tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGCATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                       >  2:762463/1‑149 (MQ=255)
               tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGccc                                                                                                                                       <  1:345458/149‑1 (MQ=255)
               tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                       <  2:1012172/149‑1 (MQ=255)
                 ccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAAAGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                     >  1:174174/1‑149 (MQ=255)
                 ccctgccATCGAAAATCCCCATTAAATAAACACCACGCATTGATATGACATTGATTTATATTCTGCAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                     <  1:774610/149‑1 (MQ=255)
                     gccATCGTAAATCCCCATTAAATAAACACACCGCCTTGCTCTGACTTTGCTTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGGGGAGCAGGGATAAAAAAACGGGCAGGGCACTGACTTGCCCGttttt                                                                                                                                 <  1:755397/149‑4 (MQ=255)
                     gccATCGTAAATCCCCATTAAATAAACACAACGCAGTGATCTGACTTTGAGTTATTTTCTGGAGCAGACTCGCAAAGGAGAATGCGCACCGCGGCAACGGTGAGGAGAAGGGATAAAAAAACGGGCAGGGCACTGACTTGCCCGttttt                                                                                                                                 <  1:498620/149‑4 (MQ=255)
                       cATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGCCCTGCCCGTTGAttt                                                                                                                               >  2:115788/1‑149 (MQ=255)
                       cATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                                                               >  2:153022/1‑149 (MQ=255)
                        aTCGTAAATCCCCATTAAATAATCACAACGCATTGATCTGACATTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCATCGCGGCAACTGTGTGGAGAACTGTTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttgt                                                                                                                              >  1:697048/1‑147 (MQ=255)
                        aTCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                                                                                 >  2:375982/1‑130 (MQ=255)
                        aTCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                                                                                 <  1:375982/130‑1 (MQ=255)
                          cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                            <  2:380281/149‑1 (MQ=255)
                          cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGAAGTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                            <  2:582180/149‑1 (MQ=255)
                           ttAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                           <  1:1173850/148‑1 (MQ=255)
                           gTAAATCCCCATTAAAAAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGACTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                           <  1:1178262/149‑1 (MQ=255)
                                 ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                                     <  2:48575/149‑1 (MQ=255)
                                  cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                                                                    <  1:1099977/149‑1 (MQ=255)
                                   ccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCAGTTGATTTTCAGAGAAgggg                                                                                                                   >  2:14375/1‑149 (MQ=255)
                                   ccATTAAATAAACACAACGCATTGATCCGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                                   <  1:1132006/149‑1 (MQ=255)
                                    cATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                                  <  2:387339/149‑1 (MQ=255)
                                          ataaaCACAACGCATTTATCTGACTTTGATTTATTTTCTGGAGCAGACACGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGCAAAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                            >  1:552806/1‑149 (MQ=255)
                                             aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                                                         <  2:1034508/149‑1 (MQ=255)
                                              acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAccg                                                                                                        >  2:220731/1‑147 (MQ=255)
                                              acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                        >  1:1035691/1‑149 (MQ=255)
                                               aacaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGTATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                                                                       <  1:816529/148‑1 (MQ=255)
                                                      cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                                                                <  1:228462/149‑1 (MQ=255)
                                                       aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgc                                                                                               >  1:388220/1‑149 (MQ=255)
                                                         tGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcgc                                                                                             <  1:390689/149‑1 (MQ=255)
                                                            tCTGACTTTGATTTTTTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACTGTCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGTGGAATTAGTACAGCAGACGGGCGCGaa                                                                                          <  2:495794/149‑1 (MQ=255)
                                                                aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGt                                                                                      >  1:1122259/1‑149 (MQ=255)
                                                                  tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                          >  1:565853/1‑127 (MQ=255)
                                                                  tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                          <  2:565853/127‑1 (MQ=255)
                                                                  tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                                                                    >  1:1150512/1‑149 (MQ=255)
                                                                   tttatttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                                                                   <  1:471786/146‑1 (MQ=255)
                                                                    tGATGTATTTTCTCGAGCAGACTCCCAAAGTAGAATCCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAATCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGCGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAccc                                                                                  <  2:54461/149‑1 (MQ=255)
                                                                     tatttatttTCTGGAGCAGACTCGCAAAGTCGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                 <  2:68200/148‑1 (MQ=255)
                                                                     gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                 <  2:1122259/149‑1 (MQ=255)
                                                                     gATTTATTTTCTGGAGCAGACTCGCAAAGAAGAATGCGCAACGCGGCAACGGGGTGGAGAAGGGATAAAAAAACGGGCAAGGCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCg                                                                                 <  1:288338/149‑1 (MQ=255)
                                                                      atttatttTCTGGAGCAGCCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGTTAAAAAAACGGGCAAGTCAGTGACCTGCGCGGTGATTTTCAGAGAAGGGGATGTAGTACAGCAGCCGGGCGCGAATGGTACCCgg                                                                                >  1:880129/1‑149 (MQ=255)
                                                                      atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAATCGGGCAAGTCAGTGCCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGGAGACGGGCGCGAATGGTACCCgg                                                                                >  1:1061903/1‑149 (MQ=255)
                                                                      atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                                                                >  2:236018/1‑149 (MQ=255)
                                                                                  gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                                                                    >  1:125516/1‑149 (MQ=255)
                                                                                   atcAGACTCGCAAAGTAGACTGCTCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAACTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                                                                   <  2:813921/147‑1 (MQ=255)
                                                                                     cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                 <  2:692557/149‑1 (MQ=255)
                                                                                     cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                 >  2:760018/1‑149 (MQ=255)
                                                                                     cAGACGCTCAACGTAGAATGCGCAACGCGGCAACGGTGTGGATAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc                                                                 <  2:293957/149‑1 (MQ=255)
                                                                                      aGACTCGCATAGTAGAATGCGCACCGCGGCATCGGTGTGGAGATGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGTCGCGAATGGTACCCGGTATAGCTTTCATTGcc                                                                <  1:543868/149‑1 (MQ=255)
                                                                                      aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGAGTGGAGAAGTGATAAAAAAACGCTCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                                                                <  1:672406/149‑1 (MQ=255)
                                                                                       gACTCGCAAAGTAGCATGCGCAACGCGGCAACGGTGTTGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATATTCAGAGATGGGGAATTAGTACAGGAGACGGGCGCGTATGGTAACCGGAATAGCTTTCATTGCCt                                                               >  1:661972/1‑149 (MQ=255)
                                                                                       caCTCGCACAGGAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAACCGGGCACGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                                                               <  1:852388/148‑1 (MQ=255)
                                                                                        aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGATGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGTTTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                              <  1:770677/149‑1 (MQ=255)
                                                                                        aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                              <  1:55316/149‑1 (MQ=255)
                                                                                        aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                              <  1:492924/149‑1 (MQ=255)
                                                                                         ctctCAAAGTAGAATGCGCATCGCGGCAACGTTGTGGAGTAGGGATTAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGTAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                                                             <  1:1186117/149‑1 (MQ=255)
                                                                                          tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                            <  2:469819/149‑1 (MQ=255)
                                                                                            gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                                                          <  2:1035691/149‑1 (MQ=255)
                                                                                               aaGTAGAATGCGCAACGCGGCAACCGTGTGGACAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                                                       <  2:667678/149‑1 (MQ=255)
                                                                                                    gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCCGCAGCGCTTTTTc                                                  <  1:414728/149‑1 (MQ=255)
                                                                                                     aaggtgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAACCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg                                                 <  1:521460/144‑1 (MQ=255)
                                                                                                     aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGATTTAGTACAGCAGACGGGCGCGAATGGAACCCGGAATAGCTTTCATTGCCTGCAGCGCTTGTTCg                                                 >  2:567772/1‑149 (MQ=255)
                                                                                                     aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg                                                 >  2:563322/1‑149 (MQ=255)
                                                                                                         cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCaa                                             >  1:932923/1‑149 (MQ=255)
                                                                                                          gcAACGCGGCAACGGGGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTGCAGAGAAGGTGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGTCTTAGCTTTCTTTGCCTGCTGCGCTTTTTCGGCAAc                                            >  1:666176/1‑149 (MQ=255)
                                                                                                           cAACGCGGCAACTGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACg                                           <  2:748500/149‑1 (MQ=255)
                                                                                                              cgcgGCAAGGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCt                                        <  1:335637/149‑1 (MQ=255)
                                                                                                                 ggCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTTCCTGCAGCGCTTTTTCGGCAACGTCTtcg                                     >  1:943361/1‑149 (MQ=255)
                                                                                                                  gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTACAGAGAAGGGGAATTAGTACAGCAGACGGGCCCGAATGGAACCCGGAATAGCTTTCATTGCCTGCAGCGATTATTCGGCAACGACTtcgt                                    >  2:563561/1‑149 (MQ=255)
                                                                                                                        gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTTGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                              <  2:174174/149‑1 (MQ=255)
                                                                                                                         tgtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCTGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtta                             >  2:1006504/1‑148 (MQ=255)
                                                                                                                            ggAGAACGGATAAAAAAACGGGCAAGTCAGTGACGTGGCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGGGCTTTTTCGGCAACGTCTTCGTCGGCTtcaat                          <  1:800325/149‑1 (MQ=255)
                                                                                                                             gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaata                         <  1:742683/149‑1 (MQ=255)
                                                                                                                               gaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatc                       <  1:153022/149‑1 (MQ=255)
                                                                                                                                 aGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaa                     <  1:14375/149‑1 (MQ=255)
                                                                                                                                  gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                    <  2:552806/149‑1 (MQ=255)
                                                                                                                                  gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat                    >  2:52088/1‑149 (MQ=255)
                                                                                                                                  gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTGCGTCGGCTtcaatatcaat                    >  2:1098501/1‑149 (MQ=255)
                                                                                                                                      aaaaaaaaCGGCGCAAGGCCGTCACCTGCCCGTTGATTTTCAGAGCAGGGGAATTAGAACAGCAGACGGGCGCGATTGGTTCCCGGAATAGCTTTCATTGGCTGCAGCGCTTTTTCGGCATCGGCTTCGTCGGCTTCAATATCAATAAc                 <  2:333038/148‑1 (MQ=255)
                                                                                                                                         aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACcaca             >  2:866704/1‑149 (MQ=255)
                                                                                                                                           caaCGGGCAGGTCACTGACTTGCACGTTGATTTTCAGAGAAGGGGAATAACTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCACCGTATTCGTCGGCTTCAATATCAATAACcacaga           >  1:241352/2‑147 (MQ=255)
                                                                                                                                            aaCGGGCAAGTCAGAGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGCCGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCGTCAATATCAATAACCACATaa          >  1:55943/1‑149 (MQ=255)
                                                                                                                                             aCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAAc         <  2:152446/149‑1 (MQ=255)
                                                                                                                                               gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAAccc       <  2:2617/149‑1 (MQ=255)
                                                                                                                                                  gCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCAt     >  2:1109839/1‑149 (MQ=255)
                                                                                                                                                  gCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCGTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATTACCCAt     >  2:1105855/1‑149 (MQ=255)
                                                                                                                                                   cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATc    >  1:1035508/1‑149 (MQ=255)
                                                                                                                                                     aGTCAGTGACCGGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTg  <  2:1087845/149‑1 (MQ=255)
                                                                                                                                                     aGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTg  <  1:760018/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTACAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTG  >  NC_000913/3056992‑3057288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: