Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,172 C→G 6.2% N184K (AAC→AAG)  accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,1720CG 6.2% 162.0 / 2.4 60N184K (AAC→AAG) accAacetyl‑CoA carboxylase, carboxytransferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base G (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTG  >  NC_000913/209024‑209320
                                                                                                                                                    |                                                                                                                                                    
cAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAAc                                                                                                                                                      >  1:931086/1‑149 (MQ=255)
       aGAAGGTTACCGCAAAGCACTGCGTATGATGCAAATGGCTGACCGCGTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTg                                                                                                                                               <  2:688672/149‑1 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGa                                                                                                                                              <  2:931086/149‑1 (MQ=255)
        gAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGGGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGa                                                                                                                                              <  1:872282/149‑1 (MQ=255)
          aGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaaa                                                                                                                                            <  2:719964/149‑1 (MQ=255)
          aGGTTACCGCAAAGCACTGCGGCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTCTCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGaaa                                                                                                                                            <  2:1064778/149‑1 (MQ=255)
              tACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtc                                                                                                                                        <  2:1165531/149‑1 (MQ=255)
               aCCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTAACGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGACAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtct                                                                                                                                       <  1:740666/149‑1 (MQ=255)
                ccGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGtctc                                                                                                                                      <  1:163195/149‑1 (MQ=255)
                 cgaaaaGCACTCCGTCTGATGCAAATTGCTGAACGCTTTTAGTTGCCTATCATGACCTTTATCGACACCCCGTTGGCTAATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                     <  1:821371/146‑1 (MQ=255)
                 cGCACAGCACTGCTTCTGAAGCAAATGGCTGAACGCGTTAAGATGCCTATCATCACCATTATCGACACCCCTGGGGCTTATCCTGGCGTTGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTTCACGCAGGTTGCGTGCAATGgcttc                                                                                                                                     <  1:1144412/149‑3 (MQ=255)
                 cGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCg                                                                                                                                     >  1:1176093/1‑149 (MQ=255)
                                 tGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTAATCGACACCCCGGCGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTa                                                                                                                     <  1:1037408/149‑1 (MQ=255)
                                        aaTGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTa                                                                                                              >  2:199079/1‑149 (MQ=255)
                                         aTGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTAc                                                                                                             >  1:732325/1‑149 (MQ=255)
                                           ggCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACgg                                                                                                           <  2:72273/149‑1 (MQ=255)
                                              tGAACGCTTTAAGATGCCTATCATCACCTTTATTGACACACCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTa                                                                                                        <  1:6798/149‑1 (MQ=255)
                                              tGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTa                                                                                                        >  1:962880/1‑149 (MQ=255)
                                                aaCGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGGCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATc                                                                                                      <  1:901076/149‑1 (MQ=255)
                                                     tttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTTGTTTGTTCGGTTATCGGTGa                                                                                                 >  2:332665/1‑149 (MQ=255)
                                                     tttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGa                                                                                                 >  1:456730/1‑149 (MQ=255)
                                                     tttAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACATGCGTGAAATGTCTCGCCTCGGCGTCGCGGTAGGTTGTAAGGTTATGGGTGa                                                                                                 >  2:641232/1‑149 (MQ=255)
                                                       tAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAg                                                                                               <  1:433726/149‑1 (MQ=255)
                                                        aaGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTTGTTTGTACGGTTATCGGTGAAgg                                                                                              >  1:406438/1‑149 (MQ=255)
                                                        aaGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAgg                                                                                              >  1:888298/1‑149 (MQ=255)
                                                         aGATGCCTATCATCACCTTTATCGACACCCCGGGTGCTTATCCTGGCGTGGCCGCAGAACAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAAATTCACGCCTCTGCGTACAGGTAGTTTGATCGGTTAGCGCTGATggt                                                                                             >  1:778789/1‑149 (MQ=255)
                                                               cTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCt                                                                                       >  2:761624/1‑149 (MQ=255)
                                                                    ctgaCCTTGATCGCCCCCCCGGGGGCGTATCCTGGCGTGGGCGCAGCAGAGCGTGGTCAGTCTGAAGCCAGTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGATATCGGTGAAGGTGGGTCTGGCgg                                                                                  <  2:767608/146‑1 (MQ=255)
                                                                              tCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCg                                                                        >  1:916005/1‑149 (MQ=255)
                                                                               cGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGa                                                                       >  1:343145/1‑149 (MQ=255)
                                                                                 acacCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCAGTGAAGGTGGTTCTGGCGGTGCGCTGGCGAtt                                                                     <  2:962880/149‑1 (MQ=255)
                                                                                   acCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTGCTGGCGGTGCGCTGGCGATTgg                                                                   <  1:167774/149‑1 (MQ=255)
                                                                                     cccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTCCGGGTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCg                                                                 >  1:1043093/1‑149 (MQ=255)
                                                                                      ccGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTAACGGTATTTTGTACGTGTAGCGGTGACGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGt                                                                >  2:779076/1‑149 (MQ=255)
                                                                                       cGGGGGCTTATACTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTTGTTCTGGCGGAGCGCTGGCGATTGGCGTg                                                               >  2:6852/1‑149 (MQ=255)
                                                                                        gggggCTTATCCTCGCGGGGGCGCAGCAGTGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTgg                                                              <  1:1040752/149‑1 (MQ=255)
                                                                                              ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTCGCGTGGGCGATa                                                        >  1:891110/1‑149 (MQ=255)
                                                                                              ttATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTGATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATa                                                        >  1:407046/1‑149 (MQ=255)
                                                                                                        gTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGAGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAatat                                              >  1:295196/1‑149 (MQ=255)
                                                                                                            gcgcATAAGAGCGTTGTCAGTCTGTAGCAATTTCAAGCAAGCTGCGTGAATTTTCTCGCCTATGCGTACCTGTAGTTTTTACGGTTATCTGTTAAGGTGGTTATTGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctg                                          <  2:732325/149‑1 (MQ=255)
                                                                                                            gcgcAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATAtgctg                                          <  2:139585/149‑1 (MQ=255)
                                                                                                                   agagCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTCGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACa                                   >  1:266483/1‑149 (MQ=255)
                                                                                                                       ggTGGTCAGGCTTAAGCCATTGCACCCTACCTGCCTGAAATGTCGCGCCTCCGCGTACCCGCCGTCTGTCCGGATAGCGGGGCAGGTGCGGCTGGCGCTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATCCAGCAc                               <  2:302066/148‑1 (MQ=255)
                                                                                                                          ggTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTa                            >  2:909074/1‑149 (MQ=255)
                                                                                                                            tCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCATTACAGCACCTAtt                          >  2:1116196/1‑149 (MQ=255)
                                                                                                                               gTCTGAAGCCCTTGCACGCCACCTGCGGGAAATGTCTCGCCGCGGCGTACCGGGAGGATGTACGGTAAACGGTGAAGGGCGTGCTGGCGGAGCGCCGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCg                       <  2:868723/149‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCATCCTGCGTGAAAAGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGGGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCg                       <  2:572984/149‑1 (MQ=255)
                                                                                                                               gTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCACGGCGTCCCGGTAGTTTGTACGGTTATCGGTGCAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCAATTCCg                       <  2:881709/149‑1 (MQ=255)
                                                                                                                                tCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGATGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGAACCTATTCCGt                      >  2:988229/1‑149 (MQ=255)
                                                                                                                                tCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGATTATGCTGCAATACAGCAACTTTTCCGt                      >  1:818365/1‑149 (MQ=255)
                                                                                                                                tCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGt                      <  1:437140/149‑1 (MQ=255)
                                                                                                                                 cTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGtt                     <  2:617810/149‑1 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCACCCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGATGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCCCCTATTCCGTTAt                   >  2:619971/1‑149 (MQ=255)
                                                                                                                                   gAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAt                   >  1:85651/1‑149 (MQ=255)
                                                                                                                                    gagacATTTCACGCAGGTTGCGTGCAATGGCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtg                  >  1:524104/5‑148 (MQ=255)
                                                                                                                                    aaGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTAtc                  >  2:524227/1‑149 (MQ=255)
                                                                                                                                    aaGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGGTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCGATTCCGTTAtc                  >  1:212572/1‑149 (MQ=255)
                                                                                                                                      gCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTGCGTTAtctc                >  2:514799/1‑149 (MQ=255)
                                                                                                                                       acATTTCACGCAGGTTGCGTGCAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTAACGGTGCAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATATCg               >  2:84186/2‑149 (MQ=255)
                                                                                                                                              aCGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAg        >  1:274915/1‑149 (MQ=255)
                                                                                                                                                 cAACCTGCGTGAAATGTCGCGCCTCCTCGTACCGGTCGATTGGACGGTTATCGGTGAAGGTGGATCTGGGGGTGCGCAGGCGATTGGCGTGGGCGATAAAGTTAATATGCTGCAATACCGCACCTATTCCGTAATCTCCCGGGAATGtt     <  2:945071/149‑1 (MQ=255)
                                                                                                                                                    ccTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTtgtg  <  1:761624/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTTATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCCATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATCGGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTGCAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTG  >  NC_000913/209024‑209320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑