Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,079,856 C→G 6.9% A594P (GCT→CCT)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,079,8560CG 6.9% 289.8 / 7.1 103A594P (GCT→CCT) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (45/50);  new base G (4/3);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCT  >  NC_000913/3079708‑3080004
                                                                                                                                                    |                                                                                                                                                    
agcTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAagcagc                                                                                                                                                      <  2:575719/149‑1 (MQ=255)
   tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTTACCGCTGTCGGCAGTACGGATTCAAGGTAAGCAGCATc                                                                                                                                                   >  1:901284/1‑149 (MQ=255)
   tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATc                                                                                                                                                   <  2:1106392/149‑1 (MQ=255)
   tctGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACACGCTTCTACAGCACCGCGTGCAGTACCCGCTTTCGGCAGTACGGCTTCACGGTAAGCAGCATc                                                                                                                                                   >  2:596641/1‑149 (MQ=255)
       ccGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGc                                                                                                                                               <  1:122119/149‑1 (MQ=255)
          gaggagATTCTCCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGGCAGCAATACCCGCTTCTACAGCAACGCGTGCAGAAACCGCATTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTg                                                                                                                                            <  1:614250/149‑1 (MQ=255)
            gCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGGATGCTGCttacc                                                                                                                                          <  1:561335/149‑4 (MQ=255)
            gCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGGATGCTGCttacc                                                                                                                                          <  1:356607/149‑4 (MQ=255)
            gCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGGATGCTGCttacc                                                                                                                                          <  1:406497/149‑4 (MQ=255)
              aGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGCTAAGCAGCATCCTGCTTGTCaa                                                                                                                                        >  2:267414/1‑149 (MQ=255)
                aTTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAAt                                                                                                                                      <  2:662096/149‑1 (MQ=255)
                aTTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAAt                                                                                                                                      <  1:1133512/149‑1 (MQ=255)
                aTTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAAt                                                                                                                                      >  1:247948/1‑149 (MQ=255)
                aTTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGGGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAAt                                                                                                                                      >  2:911086/1‑149 (MQ=255)
                 ttCACCGCAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATg                                                                                                                                     <  2:208710/149‑1 (MQ=255)
                 ttCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATg                                                                                                                                     >  1:985250/1‑149 (MQ=255)
                  tCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGGGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCGTCCTGCTTGTCTAATGc                                                                                                                                    >  2:594962/1‑149 (MQ=255)
                  tCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGc                                                                                                                                    <  1:32077/149‑1 (MQ=255)
                  tCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGc                                                                                                                                    <  1:762953/149‑1 (MQ=255)
                    aCCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGt                                                                                                                                  <  2:396168/149‑1 (MQ=255)
                     ccGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTc                                                                                                                                 >  1:163367/1‑149 (MQ=255)
                      cGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCTGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCg                                                                                                                                <  1:993592/149‑1 (MQ=255)
                      cGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCg                                                                                                                                >  1:657831/1‑149 (MQ=255)
                      cGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCg                                                                                                                                <  2:962909/149‑1 (MQ=255)
                        aaGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCTTCGGt                                                                                                                              >  2:719749/1‑149 (MQ=255)
                         aGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTa                                                                                                                             <  1:559168/149‑1 (MQ=255)
                           gtggtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCCGCTTCCTGCTTGTCAAATGCGTCGGTAGa                                                                                                                           >  2:489012/1‑149 (MQ=255)
                             ggtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACGCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCTTCCTGCTTGTCAACTGCGTCGGTAGACg                                                                                                                         <  2:113259/149‑1 (MQ=255)
                              gtCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAAAACCCGCATCTACAGCAACGAGAGCAGTAACCGCTATCGGCAGAACGGAGTCACCGTAAGCAGCATCCAGCTTGTCAAATGCGTCGGTAGACgg                                                                                                                        >  1:298139/1‑149 (MQ=38)
                               tCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCTCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGc                                                                                                                       <  1:1176092/149‑1 (MQ=255)
                               tCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGc                                                                                                                       <  1:246093/149‑1 (MQ=255)
                                    ccGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa                                                                                                                  >  2:1082342/1‑149 (MQ=255)
                                     cgacgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTAAGCAATCCCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCTGTACGGATTCACGGTAAGCAGCATCCTGCGTGTCAAATGCGTCGGTAGACGGCATGGac                                                                                                                 >  1:899634/1‑149 (MQ=255)
                                       acgaTTGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGATTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGacac                                                                                                               <  2:36292/149‑1 (MQ=255)
                                       acgaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGacac                                                                                                               <  1:1138257/149‑1 (MQ=255)
                                         gaTAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTGGTCAAATGCGGCGGTTGACGGCATGGAcaccc                                                                                                             >  2:1045304/1‑148 (MQ=255)
                                           tAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                                                                           <  2:182648/149‑1 (MQ=255)
                                              cACCGTTCAGGCCACCATACTGGTACCAGTAGTCAGAAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCAGGGTAAGCAGCATCCTGCTTGTCAAATGCGGCGGTAGACGGCATTGACACCAcgcgc                                                                                                        <  2:899265/149‑1 (MQ=255)
                                              cACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                                                                                                        >  1:152481/1‑149 (MQ=255)
                                               aCCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACCCCAcgcgcg                                                                                                       >  2:96296/1‑149 (MQ=255)
                                                   ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                                                                   >  2:43417/1‑149 (MQ=255)
                                                   ttCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                                                                   <  1:552068/149‑1 (MQ=255)
                                                      aGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCAc                                                                                                <  1:715137/149‑1 (MQ=255)
                                                      aGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCAACAGCACCGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCGTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCCCGCGCGCGTTCAc                                                                                                >  1:819589/1‑149 (MQ=255)
                                                        gCCAACATACTTGTACGAGTAGTCAGCAATACACGCTTCTACAGCAACGCGTGCAGTAACAGCGTTCGGCAGTACGGATTCACGGTAAGCAGGATCCTGCTTGTCAAATGCGTCGGTAGACTGCATGGAAACCACGCGCGCTTTCACGc                                                                                              >  1:551946/1‑149 (MQ=255)
                                                         ccAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGcc                                                                                             <  1:433410/149‑1 (MQ=255)
                                                           aaCATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCtt                                                                                           <  2:871959/149‑1 (MQ=255)
                                                           aaCATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGGCAAATGCGTCGGTAGCCGGCATGGACACCACGCGCGCTTTCACGCCtt                                                                                           >  2:1059127/1‑149 (MQ=255)
                                                              aTACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCgg                                                                                        <  1:170662/149‑1 (MQ=255)
                                                              aTACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCgg                                                                                        <  1:1157525/149‑1 (MQ=255)
                                                                aCTTGTACCAGTAGTCAGCAATACCCTCTTCAACAGCAACGCGTGCAGTAACCGCTTTAGGCAGTACGGATTCACGGTAAGCAGTATCCTGCTTGTCAAATGCGTCGGTAGACGGCATTGACACCACGCGCGCTTTCACGCCTTCGGca                                                                                      <  1:898844/149‑1 (MQ=255)
                                                                aCTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                                                                      <  1:298754/149‑1 (MQ=255)
                                                                   tGTACCAGTAGTCCGCAATCCCCGCTTCTACAGCCACGCGTGCAGTAACCGCTTTCGGCAGTCCGGATTCACGGTAAGCAGCATCCTGCTTGTCATATGCGTCGGTATACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtc                                                                                   <  2:553968/149‑1 (MQ=255)
                                                                    gTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtca                                                                                  <  1:176668/149‑1 (MQ=255)
                                                                       ccAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                                               >  1:659005/1‑149 (MQ=255)
                                                                         agtagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCCCGCCTTCGGCCGTCAGtttt                                                                             >  2:659541/1‑149 (MQ=255)
                                                                         agtagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtttt                                                                             <  1:927734/149‑1 (MQ=255)
                                                                          gtagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                                                            >  2:472310/1‑149 (MQ=255)
                                                                          gtagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                                                            <  1:993847/149‑1 (MQ=255)
                                                                          gtagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTATCACGCCTACGGCAGTCAGtattt                                                                            >  1:533315/1‑149 (MQ=255)
                                                                           tagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc                                                                           <  2:521184/149‑1 (MQ=255)
                                                                           tagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc                                                                           <  1:583889/149‑1 (MQ=255)
                                                                           tagtCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc                                                                           <  2:247948/149‑1 (MQ=255)
                                                                              tCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTa                                                                        <  1:50317/149‑1 (MQ=255)
                                                                              tCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGGACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTa                                                                        >  2:551029/1‑149 (MQ=255)
                                                                              tCAGCAATACCCGCTTCTACAGCAACCCGTGCAGTAACCGCTTTCGGCAGTACGGATGCACGGTAACCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTa                                                                        <  2:110013/149‑1 (MQ=255)
                                                                                cgCAATACCCGCGTCTCCAGCAAAGCGTGCAGTAACCGCTTTCGGCAGTACGGAGGCACGGTAAGCAGGATCCTGGTTGTCAAATGCGGGGGTAGCGGGGATGGACCCCTGGCGCGATGTCGCGCGGTGGGCTGTCAGTTGATCGTAgg                                                                      >  2:511422/2‑149 (MQ=255)
                                                                                 gCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCGTCGGCAGTCAGGTTTTCGTTGgc                                                                     >  1:963286/1‑149 (MQ=255)
                                                                                        ccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACa                                                              >  2:1114437/1‑149 (MQ=255)
                                                                                          gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGc                                                            <  1:167381/149‑1 (MQ=255)
                                                                                             tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAg                                                         <  2:163367/149‑1 (MQ=255)
                                                                                             tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAg                                                         >  1:556896/1‑149 (MQ=255)
                                                                                             tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCGTTCACGCCGTCGGCAGTCAGTTTATAGTAGGCAGCAccagccag                                                         >  1:713593/1‑149 (MQ=255)
                                                                                               tACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATGCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGtt                                                       <  1:824439/149‑1 (MQ=255)
                                                                                                aCAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTc                                                      >  1:1128966/1‑149 (MQ=255)
                                                                                                  aGCAACGCGTGAAGTAACCGCTTTCGCCAGTACGGATTTACGGTAAGCAGCATCCTGCTTGTCAAATGCGACGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCGGTCATTTTTTCGTTGGCAGCAACCGCCAGTTCaa                                                    >  2:876766/1‑149 (MQ=255)
                                                                                                    cAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCATCt                                                  >  1:938012/1‑149 (MQ=255)
                                                                                                         cgTGCACTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCAGCCTGCGTGTCAACTGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGa                                             <  2:92142/149‑1 (MQ=255)
                                                                                                              aGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCTTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTACGGCAGTCAGTTTTTCGTAGTCAGCAACAGCCAGTTAAACTACTGAACCgg                                        >  1:1044539/1‑149 (MQ=255)
                                                                                                              aGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGAAGGCATGGACACCACGCTCGCTTTCACGCCTTCGGCAGTCAGTGTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCgg                                        >  2:316367/1‑149 (MQ=255)
                                                                                                                tAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTa                                      <  2:399555/149‑1 (MQ=255)
                                                                                                                 aaCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCTACAGCCAGTTCAACTGCTGCACCGGTAg                                     >  1:256039/1‑149 (MQ=255)
                                                                                                                 aaCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAg                                     <  2:659005/149‑1 (MQ=255)
                                                                                                                    cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACCCCACGCGCGCTTTACCGCCTTCGGCAGTCAGTTTTTCGTAGGCCGCACCAGCCAGTTCAACTTCGGAACCGGTAGCGa                                  >  1:1149331/1‑149 (MQ=255)
                                                                                                                    cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATACTGCTTGTCAAATGCGTCGGTAGCCGGCATGCACCCCACGCGCGCTTTCACGCCTTCGGCAGTCAGATTTTCGTAGGCAGAAACACCAAGTAAAACTTCTGAACCGGTAGCGa                                  >  2:874123/1‑149 (MQ=255)
                                                                                                                          cGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGaaaa                            <  1:267414/149‑1 (MQ=255)
                                                                                                                           ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAAt                           >  2:845211/1‑149 (MQ=255)
                                                                                                                              aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAg                        >  2:1154794/1‑149 (MQ=255)
                                                                                                                                  cGGAATCACGTTAAGCAGCATCCTGCTTGTCAAATGCGTCTCTAGACGGCATGGACACCACTCGCGCTTTCACGCCTTCTGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTTAACCGGTAGCGATGAAAATCAGTTcc                    <  2:848938/149‑1 (MQ=255)
                                                                                                                                   tgATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACTCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCg                   <  1:784428/148‑1 (MQ=255)
                                                                                                                                    gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCgg                  <  2:842411/149‑1 (MQ=255)
                                                                                                                                    gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCgg                  <  2:85352/149‑1 (MQ=255)
                                                                                                                                    gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCgg                  <  2:29709/149‑1 (MQ=255)
                                                                                                                                        tttgacaaGCAGGATGCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGa              >  1:844756/7‑149 (MQ=255)
                                                                                                                                         aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGGAGCGATGCAAATCAGTTCCGGATGAc             >  1:241715/1‑149 (MQ=255)
                                                                                                                                             caaGCAGGATGCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGCAAATCATTTACGGCTGACCGgc         >  1:801150/2‑149 (MQ=255)
                                                                                                                                                gcagcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCa      <  1:1082342/149‑1 (MQ=255)
                                                                                                                                                   gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCgg                  <  1:317524/134‑1 (MQ=255)
                                                                                                                                                   gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCgg                  >  2:317524/1‑134 (MQ=255)
                                                                                                                                                    caTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACCCCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACCGCCAGTTCACCTTCTGCACCGGTAGCGATGAAAATCAGTTCCGGCTGCCCGGCGCCGTCt  >  1:319771/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCTCTGCCGGAGCAGATTCACCGAAGGTGGTCATACCGACGATAGCACCGTTCAGGCCAACATACTTGTACCAGTAGTCAGCAATACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCT  >  NC_000913/3079708‑3080004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: