| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,171,863 | A→G | 6.6% | intergenic (+30/+16) | qseC → / ← ygiZ | quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,171,863 | 0 | A | G | 6.6% | 163.0 / 3.0 | 61 | intergenic (+30/+16) | qseC/ygiZ | quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base G (2/2); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
CTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAG > NC_000913/3171718‑3172010 | cTACCTGCAGCGGGCTTGGGATATCTATTGGCCAGCTAATCGCAAAATTGCATGGCATGAATGTTGAATTTGGGAATCCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActc < 2:873228/149‑1 (MQ=255)cTACCGGCTGCTGGCTTGGGCTTTCGATTGTCCCGCTAATCGCCAAATTGCATGGCATGAATGTTCAATTTGGGAATGCGGAACAAGGTGTATTTGTGGCGAAGGTAACCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActc < 1:1020570/149‑1 (MQ=255) ggCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa < 1:21309/149‑1 (MQ=255) cAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa < 1:1150291/149‑1 (MQ=255) ggCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa < 2:57797/149‑1 (MQ=255) ggCTTGGGCTAGCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCGGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTa < 1:62141/149‑1 (MQ=255) gCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGTCATGAATGTTGAAATTGGGAATGCGGAACAAGGTTGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc < 1:541408/149‑1 (MQ=255) ttGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTa > 1:604341/1‑149 (MQ=255) gCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTTGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg > 1:204460/1‑149 (MQ=255) gCTATCCTTTGTCCAGCGAATACCCAAATTGCATGGCATGAATTTTGAATTTGGGAATGCGCAACAAGGGCGAATTGACGCGAAGGTAAGCTGGAAACTTCTCTGTCTATGCGCGCCGTTAAAACGACTCTCATAGATTTTACTAATAg < 1:324208/149‑1 (MQ=255) gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat > 1:647938/1‑149 (MQ=255) gATTGTCCAGCGAATCGCCAAATTGCATGGCAGGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTATCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAat > 1:259132/1‑149 (MQ=255) aTTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTATAAGCAAAata > 1:985728/1‑149 (MQ=255) gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAACGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaa < 2:654230/149‑1 (MQ=255) gCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt < 2:1010376/149‑1 (MQ=255) aaTCGCCAAATTGCATGTCTGGAAGGTTGAATATGGTAATGCGGAACAATGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGAAAAATATAAACCGTccc < 2:1070671/149‑1 (MQ=255) gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTTACTTCTCTGTCTATGAGAGCCGTGAAAACGACTCTCATAGATTTTACGAATAGCAAAATATAAACCGTACCCaaa > 2:713087/1‑149 (MQ=255) ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa < 1:948062/149‑1 (MQ=255) aaaTTGCATGGCATGAATGTGGAATTTGGGAATGCGCAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa < 2:264766/149‑1 (MQ=255) ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcc > 1:624358/1‑149 (MQ=255) atggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcctcca > 1:765181/1‑149 (MQ=255) atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac > 2:900790/1‑149 (MQ=255) atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac < 1:423705/149‑1 (MQ=255) atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATACATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac > 1:924841/1‑149 (MQ=255) ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAg > 1:1100054/1‑149 (MQ=255) ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATGTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAACCACCAACCACAAACCAg > 2:514193/1‑149 (MQ=255) tGAATTTGGGAATGCGGAACAAGGTTGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGACGTTAAAACGACTATCATAGATTTTACTAATTGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa < 1:1076641/149‑1 (MQ=255) gcaTTTGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGCCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAACGCCACCAACCACAAACCAGAc > 1:887786/3‑149 (MQ=255) tttGGTCATGCGGAACAAGGTGTTTTTGAGTCGAAGGAAAGCTGGTAACTTCTCTGTCTAGGAGAGCCGTTAAAACGACACTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaaa < 2:765181/149‑1 (MQ=255) tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc > 1:617232/1‑149 (MQ=255) tcGGAAGAAGGTGGATTTGATGCGATGGTAAGCTGTTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg < 1:1145526/148‑1 (MQ=255) gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg > 2:222336/1‑149 (MQ=255) gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg > 2:279130/1‑149 (MQ=255) ctgAACATGGTGGATTTGAGGCTAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga < 2:106334/147‑1 (MQ=255) cGGACCAAGGTGGATTTGATGCGAAGGTAAGCTGGTAACTGCTCTGTCTCTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTTATAGCAAAATATAAACCGTCCACAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga < 2:523090/149‑1 (MQ=255) aaCATGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAATAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa < 2:553146/149‑1 (MQ=255) aagggtgATTTTCGGCGAAGGTAAGCTGTTAACTTATCTGTCTATGAGAGCCGTTAAAACTACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc < 2:1075019/143‑1 (MQ=255) aGGTGGATTTGAGGCGAAGGTAAGCTGGTATCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAATCCGTCCCCAAAAACGCCACCAACCACAAACCAGACAAATACCCAGAGAGAATACa > 1:1031435/1‑149 (MQ=255) gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata > 2:85781/1‑149 (MQ=255) tttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAccc > 1:292923/1‑149 (MQ=255) ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAAc < 1:411688/149‑1 (MQ=255) gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt > 1:480721/1‑149 (MQ=255) gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCg > 1:336029/1‑149 (MQ=255) aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGa > 2:23492/1‑149 (MQ=255) aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCAAAAACCAGACAAATACCGAGCGAGAATAAATATACCCAAAACTCGa > 1:851139/1‑149 (MQ=255) gTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACAGAGCGAGAATACATATACCCAAAACTCGaaaa > 2:1106201/1‑147 (MQ=255) tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAAAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAt < 2:43176/149‑1 (MQ=255) aGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt < 2:608985/149‑1 (MQ=255) ctctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACGAATAGCAAAATATAAACCGTCCCCAAAAAATCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAATCTCGAACATTTCCCGCATAAaga > 1:904561/1‑149 (MQ=255) tctGTCTATGAGAGCCGATAAAACGACTCTCATAGATATTACTAATAGCAAAATATAAACCGACCCCAAAAAATCCACCAACCACAAACCAGACAAATAACGAGCGAGAATACAAATACACAAAACACGAACATTACCCTCATAAagat > 2:28359/1‑148 (MQ=255) aaatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGt > 2:1097633/4‑149 (MQ=39) tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAACCCACCAACCACAAACCAGACAAATACCCAGCGAGAATACATATAACCAAAACTCGAACATTTCCCGCAAAAAGAGtt > 2:41392/1‑149 (MQ=255) tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt > 1:27118/1‑149 (MQ=255) aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt < 2:76735/149‑1 (MQ=255) tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta < 2:878778/149‑1 (MQ=255) tagagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAACGTCCCCAAAAAATCAACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAATCCTCGAACATTTCCCGCATAAAGAGTTTTCTtaa < 1:713087/148‑1 (MQ=255) gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAAGACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa > 2:571819/1‑149 (MQ=255) tAAATCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACATATACCGACCGAGAATACATATACCCAATACTCGAACAAATCCCGCATAAAGAGTTTTCTTAATATAAGaaa < 2:69646/149‑2 (MQ=255) aaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataa < 2:604341/149‑1 (MQ=255) aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat < 2:647938/149‑1 (MQ=255) gACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAg < 2:571937/149‑1 (MQ=255) | CTACCGGCAGCGGGCTTGGGCTATCGATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAG > NC_000913/3171718‑3172010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |