Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,220 C→G 5.8% L132V (CTG→GTG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2200CG 5.8% 251.7 / 5.3 95L132V (CTG→GTG) yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (46/43);  new base G (2/3);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

CTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCA  >  NC_000913/3368072‑3368365
                                                                                                                                                    |                                                                                                                                                 
ctctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGCCACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGc                                                                                                                                                   >  2:562445/1‑149 (MQ=255)
   tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGt                                                                                                                                                >  1:1137850/1‑149 (MQ=255)
   tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACAACGTCTGCAGAATACCCATCGGCAAATATTTTGCAGACGCTGt                                                                                                                                                >  2:1125559/1‑149 (MQ=255)
    tcAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                               <  1:679092/148‑1 (MQ=255)
     cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                                                              >  1:1162049/1‑149 (MQ=255)
      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTACCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                                                                             >  2:358795/1‑149 (MQ=255)
       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                            <  2:388874/149‑1 (MQ=255)
       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCACATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                            >  1:209015/1‑149 (MQ=255)
       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGATGAAATTCACCAGACAATGTCTGTCGAATTAACACCATTGGGAATTACGCAGACACCGTCTGCATAAATCCCATCGGCAAAGATTTGTCAGACGCTGTATga                                                                                                                                            >  1:709554/1‑148 (MQ=255)
          aaCTTACGCCAATTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGGCTGGAGAATTAACACCATTGGGAATTCCGCAGACACCCTCTGCAGAATTCCCATCGTCAAAGATTTGGCAGACAGCGTCTGCCaa                                                                                                                                         <  1:1173225/149‑1 (MQ=255)
          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaa                                                                                                                                         <  2:302840/149‑1 (MQ=255)
             ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATc                                                                                                                                      >  1:743104/1‑149 (MQ=255)
             ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACCCCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATc                                                                                                                                      >  1:109578/1‑149 (MQ=255)
             ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATc                                                                                                                                      >  2:727767/1‑149 (MQ=255)
             ttACGCCAAATGAGGCTTTTTTACCTCTTATTTCAACATGTAGAAATTCACCAGACACTGTCTGGCGAATTAACTCCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTCGCAGACAGCGTCTGCCAAATc                                                                                                                                      <  1:653987/149‑1 (MQ=255)
                 gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTc                                                                                                                                  <  1:183604/149‑1 (MQ=255)
                 gCCAAATGAGGCTTTTGTACCTCTTTTTTCAACATGTTGAAATTCACAAGACAATGTATTGCGAATAAACACAATTGGGAATTACGCAGAAAACGTCTCAAGAATTCACAAAGTCAAAGAATTAGAATACATTGTCTACAAAATcgtaa                                                                                                                                  >  2:768923/1‑145 (MQ=255)
                  ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                                                                 <  1:983166/149‑1 (MQ=255)
                    aaaTGAGGCTTTTTTACATCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc                                                                                                                               <  1:215804/149‑1 (MQ=255)
                         aGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGcc                                                                                                                          >  1:752132/1‑149 (MQ=255)
                         aGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGcc                                                                                                                          >  1:147040/1‑149 (MQ=255)
                           gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCa                                                                                                                        >  1:170656/1‑149 (MQ=255)
                           gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCa                                                                                                                        >  1:1163023/1‑149 (MQ=255)
                                 ttACCTCTTTTTTCAACATGTTGAAATTCAACAGACAATGTCTGTCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCTGGTCCa                                                                                                                  <  1:1115957/149‑1 (MQ=255)
                                  tACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                                 >  2:415625/1‑149 (MQ=255)
                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCAAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                             >  2:133816/1‑149 (MQ=255)
                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAATCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCAAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                             >  2:652982/1‑149 (MQ=255)
                                       ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                            >  2:44241/1‑149 (MQ=255)
                                       ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                            >  2:628515/1‑149 (MQ=255)
                                         tttttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTc                                                                                                          >  2:864791/1‑149 (MQ=255)
                                           tttCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCg                                                                                                        >  2:867142/1‑149 (MQ=255)
                                           tttCAACAAGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGCATTGCCATCGGCAAAGAATTGGCAGCCGGTGTCTGCCAAATCTTTACCCTTGCCCACGTGCACCTACGTCCg                                                                                                        >  2:1159346/1‑149 (MQ=255)
                                             tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGtt                                                                                                      >  2:1086767/1‑149 (MQ=255)
                                                        aaaTTCACCAGACAATGTCTGGCGAATTTACACCATTGGGAAATCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGtt                                                                                           <  2:316155/149‑1 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaa                                                                                         <  1:460161/149‑1 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaa                                                                                         <  2:752132/149‑1 (MQ=255)
                                                              aCCAGTCAATGTCTGGCGAATTATCATCATTGGGTATTCGGCAGACACCGTCTGAAGAATTCCCATCGGCAAAGTTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAAc                                                                                     <  2:953060/149‑1 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                    <  2:59051/149‑1 (MQ=255)
                                                                cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACTCCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCGAATTCTTTCCCCGTGCCCGGGGACGCCTACGGGCGTTTAGTGTCCGGTatgagcgc                                                                                   >  2:178222/1‑142 (MQ=255)
                                                                cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                   <  1:823925/149‑1 (MQ=255)
                                                                cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                   <  1:405340/149‑1 (MQ=255)
                                                                     aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACg                                                                              >  2:914282/1‑149 (MQ=255)
                                                                     aaTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACg                                                                              >  2:909521/1‑149 (MQ=255)
                                                                      aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTGTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCACAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAACGCTGACGc                                                                             >  1:834137/1‑149 (MQ=255)
                                                                      aTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGc                                                                             <  1:534957/149‑1 (MQ=255)
                                                                            ggCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGCCGCCCGCAg                                                                       >  1:1011740/1‑149 (MQ=255)
                                                                                aaTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCCACGTCCGTTTACTTTCCGTTAAACACGCTGACGCCCGCAGCttt                                                                   >  1:17053/1‑149 (MQ=255)
                                                                                   tAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                                                                <  1:1181795/149‑1 (MQ=255)
                                                                                   tAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                                                                <  2:204060/149‑1 (MQ=255)
                                                                                    aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATg                                                               <  1:636536/149‑1 (MQ=255)
                                                                                     acacCATTGGGAATTCCGCATTCACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                                              <  1:703819/149‑1 (MQ=255)
                                                                                       acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                            >  2:3125/1‑149 (MQ=255)
                                                                                       acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                            <  1:845197/149‑1 (MQ=255)
                                                                                         cATTGGGAATTCAGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                                                          <  1:868340/149‑1 (MQ=255)
                                                                                           ttGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGAAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                        <  2:650227/149‑1 (MQ=255)
                                                                                             gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAgaga                                                      <  2:749891/149‑1 (MQ=255)
                                                                                                 aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCt                                                  >  2:992280/1‑149 (MQ=255)
                                                                                                   tCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                                <  2:678335/149‑1 (MQ=255)
                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGCCGCCCGCAGGTTTTATGAAAAAGAGACGCTCCGc                                              >  2:726992/1‑149 (MQ=255)
                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGc                                              <  2:147040/149‑1 (MQ=255)
                                                                                                       cAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACGTTCCGTTAAAAACGCTGACGCCCGCAGGTTTTATGAAAAAGAGACGCTCCGCtg                                            >  2:370700/1‑149 (MQ=255)
                                                                                                        aGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtgt                                           <  2:284201/149‑1 (MQ=255)
                                                                                                          acacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                         <  2:728590/149‑1 (MQ=255)
                                                                                                          acacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTACGTTTACTTTCCGTTAAAAACGCTGACGCGCGCAGCTTTTATGTTAAAGAGACGCTCCCCtgttg                                         >  2:401739/1‑147 (MQ=255)
                                                                                                             ccGTCTGCATAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGACCACCTACGTCCGTTTACTTTCCGTTAAAAACGCAGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg                                      <  2:105544/149‑1 (MQ=255)
                                                                                                             ccGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGATGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg                                      <  1:628515/149‑1 (MQ=255)
                                                                                                               gTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGCCGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                                    >  2:191555/1‑149 (MQ=255)
                                                                                                               gTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                                    <  2:931130/149‑1 (MQ=255)
                                                                                                                tCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTc                                   >  2:342200/1‑149 (MQ=255)
                                                                                                                  ggCAGAATTCCCATCGGCAAAGATATGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTg                                 <  1:97053/148‑1 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGCCGCTCCGCTGTGGCTGGTCTGtt                               >  1:565427/1‑149 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                               >  1:1019308/1‑149 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAGGAAAAAGAGCCGCTCCGCTGTGGCTGGTCTGtt                               >  2:864916/1‑149 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCAACCTACGTCCGTTTACTTTCCGTTAAAACCGCTGACGCCCGCAGCTTTTATGAAAAAGAGAAGCTCCGCTGTCGCTGGTCTGtt                               >  1:570006/1‑149 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGAGTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTCTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                               <  2:919696/149‑1 (MQ=255)
                                                                                                                     aGAATTCCCGTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTTCCCAGGTCCCCCTACGTCCGTGTACTGTCCGTTTAAACCGCTGCCGCCCCCAGCGGTTATGAAAAAGAGACGCTCCGCTGTTGCTGGTCTTTTc                              >  1:794773/1‑149 (MQ=255)
                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                              <  2:170656/149‑1 (MQ=255)
                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                              <  1:1086767/149‑1 (MQ=255)
                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                              <  1:329561/149‑1 (MQ=255)
                                                                                                                     aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACCTCCGGTTTCTTTCCGTTAAAAACGCGGACGCCCGCAGCTTTTAGGAAAAAGAGAAGCTCCGCTGTGGCTGGTCTGTTc                              >  1:1070581/1‑149 (MQ=255)
                                                                                                                      gAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCg                             >  1:757342/1‑149 (MQ=255)
                                                                                                                       aaTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCGCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGc                            <  1:946981/149‑1 (MQ=255)
                                                                                                                         agcccATCGGCAAAGATTTTGTTGCCGCGGTGTGCGAAATCTTTAAACTTGATAAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                          <  2:1185550/147‑1 (MQ=255)
                                                                                                                           cccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGc                        <  1:914282/149‑1 (MQ=255)
                                                                                                                           cccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGc                        <  1:909521/149‑1 (MQ=255)
                                                                                                                            ccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACGTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCt                       >  1:607962/1‑149 (MQ=255)
                                                                                                                                ggggaaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACTTACGTCCGTTTACTTTCCGTTAAAAACGATGACGCCCGCAGCTTTAATGAATAAGAGACGCTCCGCTGTGGCTGGTATGTTCGACAGCTagag                   >  1:852840/5‑149 (MQ=255)
                                                                                                                                 ggCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                  <  1:652982/149‑1 (MQ=255)
                                                                                                                                  gCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCg                 >  1:340901/1‑149 (MQ=255)
                                                                                                                                    aaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGCCGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGCCTGGTCTGTTCGCCAGCTAGAGAGGc               >  2:198439/1‑149 (MQ=255)
                                                                                                                                      aGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCACAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCaa             <  2:1163023/149‑1 (MQ=255)
                                                                                                                                       cctgtgtcAGACGCTTGTTGCCAAATCTTTCCCCTTGACCAGGTCCACCTACTTCCGTTTACTTTCCTTTATAAACGCTGACGCTAGCAGCTTTTATGTAAAAGAGACGCTCCGCTGTGGCTGGGCTGTTCGACAGCTAGAGCGGCaaa            <  1:768923/142‑1 (MQ=255)
                                                                                                                                          ttGGCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCATCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg         >  2:1101479/1‑149 (MQ=255)
                                                                                                                                             gCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGa      <  2:1162049/149‑1 (MQ=255)
                                                                                                                                             gCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGa      >  1:892686/1‑149 (MQ=255)
                                                                                                                                                 aCGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCa  <  2:1035014/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCA  >  NC_000913/3368072‑3368365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: