Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,101 A→C 13.7% intergenic (+84/‑114) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1010AC13.7% 170.0 / 4.1 108intergenic (+84/‑114)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (43/46);  new base C (10/7);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATT  >  NC_000913/3780953‑3781247
                                                                                                                                                    |                                                                                                                                                  
tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGa                                                                                                                                                    <  1:874858/149‑1 (MQ=255)
  cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTGACCCTAAACCTCTCCCTCACGGTGAGGGGGCCGTTCGGCGGGGTATGTTCGCCCTCACTATGAAACGaca                                                                                                                                                  >  1:596079/1‑149 (MQ=255)
  cAGGGGCTGGGTAAAGAGTCGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGaca                                                                                                                                                  <  1:614917/149‑1 (MQ=255)
    ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCTAACCCTCTCCCTCAGGGTGCGGGGGCCGTGCGGCGCTGTATGTAGACCCGCACTCGGAAACGGCAcc                                                                                                                                                >  1:654056/1‑149 (MQ=255)
     gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCTCTCCCACAAGGAGAGGGGAACCGTCGGCCCTCGATGTACTCCCTGACACTGACACGACCCCg                                                                                                                                               >  2:956210/1‑149 (MQ=255)
        cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAc                                                                                                                                            <  1:623509/149‑1 (MQ=255)
             tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                                                                                       <  1:604919/149‑1 (MQ=255)
              aatgagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                      <  1:940014/146‑1 (MQ=255)
                 gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGATCGGCGCTGTATGGACTACCTGACTCTGAAACGACACCGCACTAttttttt                                                                                                                                   >  2:740441/1‑149 (MQ=255)
                 gagGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                                   <  1:1128839/149‑1 (MQ=255)
                  agTTGCCTGAGTCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGGTGCGCCCCCTCACCCTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                                  <  2:767248/149‑1 (MQ=255)
                     tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                               <  2:733879/149‑1 (MQ=255)
                      gCCTGCGGCACTGGCTCCCATGGCGAAAGGGTATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTccc                                                                                                                              <  2:390188/149‑1 (MQ=255)
                       ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCACTCCCTCAGGGAGACGGGACCGTTCGGCGCTGTATGTACTCCCTCCCACGGCAACGCCCCCGCCCGCATTTTTTCTccca                                                                                                                             >  1:539768/1‑148 (MQ=255)
                       ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAGGGGGCCGTTCGGCGCGGTATGTGCTGCCTCACTCTGTTACGACACCGCCCGGGTTTTTTCTgccg                                                                                                                             >  1:582407/1‑148 (MQ=255)
                        cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGGCCCCGAACCCACTCCCTCAGGGAGAGGGGGCCCTTCGGGGGGGTTTGTACTGCCGCACTGGGGAACGACCCCGCCCGCTTTTTTTCTcccac                                                                                                                            >  1:300753/1‑147 (MQ=255)
                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCg                                                                                                                           >  2:36908/1‑149 (MQ=255)
                         tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTACCTCAGGGATAGGGGACCGTTAGGCGCTGTATGTACTACCTCACTCTGAAACGACACCGGACTCTTTTTTTCTCCCtag                                                                                                                           >  2:730056/1‑147 (MQ=255)
                             gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGAGAGGGGACCGTTAGGCGCGGTATGTACTCACTCACTCTGAAACGACACCGCACGCGTTTTTTCTACCACGcccc                                                                                                                       >  2:492296/1‑149 (MQ=255)
                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGACCCCTAACCCGCTCCCACCGGGAAGGGGGCCCGTTGGGCCCGGGATGTAAGCCCGCACGAAGAAACGCACCCGCCCGCGTTTTTTCTCCCTCGCCccgccg                                                                                                                   >  2:1141145/1‑149 (MQ=255)
                                   gcgcccATGGCGAAAGGGACTGCGGCATAGTCGGTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                 <  1:255037/146‑1 (MQ=255)
                                   gCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                 <  1:698684/149‑1 (MQ=255)
                                     tCCCATGGCGAAAGGGAATGTGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                               <  1:959486/149‑1 (MQ=255)
                                     tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGCGGGGGGGCCGTTCGGCGCTGTTTGTTGTCCCGCACTCTGCAACGAACCCGCCCGCGGGTTTTCTCCCTCGCCCCGCCggggg                                                                                                               >  1:375391/1‑148 (MQ=255)
                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGACCCCTAACCCTCTACCTCAGGGCGAGGGGGCCGGTCGGCGCTGGAATTCCTCCCGGACTCTGAAACGCCACCCCACTCTTTTTTTTTGCCTGGCCCCGGCggggtg                                                                                                              >  1:1008652/1‑147 (MQ=255)
                                       ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                             <  2:370862/149‑1 (MQ=255)
                                       ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                             <  2:869225/149‑1 (MQ=255)
                                       ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTACCCCAAACCCACTACCGAATGGAGCAGGGAACCTTGGGCGCAGTATTTACTACCTAACACAGAAACGACAACCCAAGCATTTTTTAACCCACGCCCCCCCGGGgaga                                                                                                             >  2:1070661/1‑149 (MQ=255)
                                       ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCAAACCCTAACCCTAAACCTCACGGAGCAGGGACCGGTAGGCGGGTTTTGTACTCCCTCACACTGAACCGCCCCCGCCCTGCTTTTTTCTCCCTAGCCCCTCCGGGgagc                                                                                                             >  2:1149129/1‑148 (MQ=255)
                                         aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAGTCCCTGACTCTGAAACGACACCGCACGCTTTTTTTATCGCTCGCCCCTCCGGGGAGAgg                                                                                                           >  2:754569/1‑149 (MQ=255)
                                          tGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                          <  1:1151660/149‑1 (MQ=255)
                                           ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                         <  1:396030/149‑2 (MQ=255)
                                             cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                       <  1:974771/149‑1 (MQ=255)
                                              gacaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                      <  1:739424/146‑1 (MQ=255)
                                               aaaGGGAATGCGGCATAGTGGTTTGCGCGCCCGCACCCTAACCCGCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCACTCTGACACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                     <  2:333494/149‑1 (MQ=255)
                                               aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                     <  1:407802/149‑1 (MQ=255)
                                                  gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGGCCGTTCGGCGCTGTATGTACTCCCGCACTCGGGAACGACCCCGCCCGCGTTTTTTGTACCGCGCCCCGCCGGGGAGGGGGGCgggggg                                                                                                  >  2:525944/1‑147 (MQ=255)
                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                 <  1:759637/149‑1 (MQ=255)
                                                     actGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                               <  1:74777/147‑1 (MQ=255)
                                                     aaTGCGCCATAGTCGTGTGCGCCGCCTCACCCTCCCCCTCTCCCTCCGCGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTCAACCGCCACCGCACTCTTTATTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                               <  2:290451/149‑1 (MQ=255)
                                                       tGCGGCATAGTCGTTTGCGCCCCCTCACCTTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg                                                                                             <  2:485354/149‑1 (MQ=255)
                                                       tGCGGCATAGTCGTTTGCGCCCCCGCCCCCTAACCCTCTCCCACAGGGAGATGGGACCGTGGGGGGCGGTAGGTACGCCCTCACTCTGAAACCACACCGCACGCTTTTTTTGGCCCGAGCCCCGGCCGGGTGCGGGCCGGGGTgggagg                                                                                             >  2:1057043/1‑144 (MQ=255)
                                                         cGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                                                                                           <  1:317337/149‑1 (MQ=255)
                                                         cGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCTCTCTGAAACCACACCGCACTCTTTTTTTCTCGCTGGCGCCTCCTGGGAGAGGGCCGGGGTGAGGGGaa                                                                                           <  1:286052/149‑1 (MQ=255)
                                                         cGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCCGACGCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGGCGGGGTGCGGGGaa                                                                                           >  1:63467/1‑149 (MQ=255)
                                                            cATAGTCGTTTGCGCCCCCTCACCCTAACCCACTCCCTCAAGGAGAGGGGACCGTTCGGCGCTGTATGTACACCCTCACTCTGAAACGCCCCCCCCCTCTTTTTTTCTCCCTCGCCCCTCCGGCGAGCGGGCCGGGGTGAGGGGAAAAg                                                                                        >  1:566433/1‑149 (MQ=255)
                                                             aTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTGTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGGCGGGGTGAGGGGAAAAgg                                                                                       >  1:831465/1‑149 (MQ=255)
                                                             aTAGTCGTTTGCGCCACCGCCCCCTACCCCTCTCCCTCCGTGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTCAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAgg                                                                                       <  1:1050268/149‑1 (MQ=255)
                                                                gtggTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCATTCTGAAACCCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCg                                                                                    <  2:743803/146‑1 (MQ=255)
                                                                       gcgcCCCCTCACCCAACCCCTCTCCCTCCGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACCACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTTAGGGGAAAAGGCCGCACTGCt                                                                             <  1:493327/149‑1 (MQ=255)
                                                                           ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAcc                                                                         >  2:169752/1‑149 (MQ=255)
                                                                           ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                               >  1:1146062/1‑127 (MQ=255)
                                                                           ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                               <  2:1146062/127‑1 (MQ=255)
                                                                              caaaCCATAACCCTCCCCCCCAGGTTGTGTGCACCGTGCGGCGCTGTATGTACTCCCTACCTCTCAAACGACCCCTCACTCTTTTTTTCTCCCTCCCCCCTCCGGGTTGTGGGCGGGGGTTTGGGGAAAAGGCCGCACTGCTCACCAAt                                                                      <  2:483524/146‑1 (MQ=255)
                                                                              cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAAt                                                                      >  1:487284/1‑149 (MQ=255)
                                                                                 aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAGTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCAAtttt                                                                   >  1:148448/1‑149 (MQ=255)
                                                                                  cccTACCCCCCTCCCTCCTGGAGAGGGGACCTTCCGGCGCTGTAGGTACCCCCTCACTCTCAAACTACACCTCACTCTTTTTAACACCCACGCCCCGCCGGTTAGAGGCCCGGGGTTAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                  <  2:78927/149‑1 (MQ=255)
                                                                                   ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTATTTTTCTCCCTCGCCCCTCCGGGGAGAGGCCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                 >  2:1107465/1‑149 (MQ=255)
                                                                                   cataccccTCTCCCGCCGGGAGAGGGGACCGTGCGGCGCTGTATGAACTCCCTCCCTCTGAAACCACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                 <  2:72321/144‑1 (MQ=255)
                                                                                     tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCGCCAATTTTCTGc                                                               >  1:763511/1‑149 (MQ=255)
                                                                                     aaaCCCTCTCCATACGGGAGTGGGAAACGTTCGTTGGTGTATGTACTCCCTCCTTCTCAAACTCCACCCCTATTTTTTTTTCTCCCTCGCCCCGCCGGGGCGTGGGCCGGGGTGTGTGTAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                               <  2:538184/148‑1 (MQ=255)
                                                                                      tccccTCTCCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAATAGGCCGCACTGCTCACCAATTTTCTGCt                                                              >  1:554061/3‑149 (MQ=255)
                                                                                      aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                              >  1:632211/1‑149 (MQ=255)
                                                                                       ccccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGTGGATAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                             >  2:126383/2‑149 (MQ=255)
                                                                                        cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                            >  1:477289/1‑149 (MQ=255)
                                                                                              cccTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTCCTCCCTCACTCTGAACCGCCGGCGCACGCTTTTGTTCTCCCTCGCCCGTCCGGGGAGAGGGCCGGGGTGAGGGGAGAAGGCCGCACTGGTCAGCACTTTTCTGCTTTCCTGcc                                                      >  2:175224/1‑149 (MQ=255)
                                                                                                     ggAGAGGGGACCGTTCGGCGCTGTATGTACCCCCTCCCTCCGAAACGACACCGCACTCTTTTTTACCCCCCCCCCCCGCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                               <  1:173798/149‑1 (MQ=255)
                                                                                                         tgggtACCTTTCGGCGCTTTTTGAACTCCCTCATTCTTAAACCACACCGCACTCTTTTTTCCACCCTCCCCCCACCGGGGAGAGGGCCGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                           <  2:582407/148‑1 (MQ=255)
                                                                                                           gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACAAAggg                                         >  1:141820/1‑149 (MQ=255)
                                                                                                           gggACCGTGCGGCGCTGTATGTACTCCCTCACTCTTAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                         <  1:539294/149‑1 (MQ=255)
                                                                                                             gACGGTTCGGCGCTGTATGCGCTCCCTCCCTCTGAACCGCCACCGCACTTTTTTTTGCTCCCTCCCCCCTCCGGGGTGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                       <  2:83280/149‑1 (MQ=255)
                                                                                                             gACCGTTCGGCGCTGTATGTACACCCTCACTCTTAAACCACCCCGCCCTCTTTTTTTCGCCCGCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                       <  2:676419/149‑1 (MQ=255)
                                                                                                              acggctCGGCGGTTTATGAACTCCCACCCTCTCAAACTCCCCCGCACTTTTTTTTTCTACCCCGCCCCTCCGGGGAGCGGGCGGGGGGGCGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                      <  2:1077986/144‑1 (MQ=255)
                                                                                                               ccGTTGGGCGCTGTAGTTACTCCCTCACTCTGAAACCACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                     <  2:831465/149‑1 (MQ=255)
                                                                                                                  ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                  >  2:932047/1‑149 (MQ=255)
                                                                                                                   tCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTTCACCCGCACTTACGGGGCAGTAt                                 >  2:653414/1‑149 (MQ=255)
                                                                                                                     ggCGCTGTTTTTACTCCCTCACTCTTAACCCACACCGCACTCTTTTATTGACCCTCGCCCCTCCGGGGTGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                               <  1:492296/149‑1 (MQ=255)
                                                                                                                      gcgtTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                              <  1:740441/149‑1 (MQ=255)
                                                                                                                       cgcTGTATCTACTCCCTCACTCTGAAACCCCACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                             <  1:37412/149‑1 (MQ=255)
                                                                                                                         ctttatgcaCTCCCTCACTCTGAAACGCCCCACCACTCTTTTTTACACCCGCGCCCCCCGGGGGAGAGGGCGGGGGGTGGGGGAAAAGGCCGCACGGCTCACCACTTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                           <  1:78451/146‑1 (MQ=255)
                                                                                                                           gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa                         >  2:167723/1‑149 (MQ=255)
                                                                                                                              tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                      >  1:675589/1‑149 (MQ=255)
                                                                                                                               tttatCCCTCACTCTGAAACTACACCGCACTTTTTTTTTCCCCGTCGCCCCTCCGGGGAGAGGGCGGGTGTTCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                     <  2:300753/145‑1 (MQ=255)
                                                                                                                               gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTACGGGGAGAGGGCCGGGGGGCGGGGAAACGGCCGCACTGCTCACCTATTTTCTGCTATCCTGCCCCCGCGCTAAGGGGGCAGTATGCTAAACATCGt                     >  2:246087/1‑149 (MQ=255)
                                                                                                                                taCTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                    <  2:13925/149‑1 (MQ=255)
                                                                                                                                taCTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCCCCCTCGCCCCCCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                    <  1:36908/149‑1 (MQ=255)
                                                                                                                                taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCAGCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCACAATTTTCTGCTATCCTGCCCCCGCACAAAGGGGGCAGTATGCTAAACATCGTa                    >  2:1070419/1‑149 (MQ=255)
                                                                                                                                 aCTCCCTCACTCTGACACGACACCGCACTCTGTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCGCACCAATTTTCTGCTATCCTGCCCCCGCACGAAGGGGGCAGTAGGCGAAACATCGTAc                   >  2:763174/1‑149 (MQ=255)
                                                                                                                                   tCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                 >  2:568732/1‑149 (MQ=255)
                                                                                                                                   tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                 >  2:466828/1‑149 (MQ=255)
                                                                                                                                     ccTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa               >  2:287366/1‑149 (MQ=255)
                                                                                                                                      ctcacactcacaCCACCCCGCACTCTTTTTACCGCCCCCGCCCCGCCGGGTTGCGGGCGGGGGGGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc              <  1:126383/149‑1 (MQ=255)
                                                                                                                                           tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc         >  1:964427/1‑149 (MQ=255)
                                                                                                                                           tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGATCACCAATTTTCTGCTATCCTCCCCCCGCACTAAGCGGGCAGTATGCTAATCAACGTACTTTACGAAcc         >  2:626233/1‑149 (MQ=255)
                                                                                                                                           tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCCGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCCTACTTTCCGAccc         >  2:381061/1‑149 (MQ=255)
                                                                                                                                             tGAACCGACCCCCCCCTCTTTTTTCCTCCCTCCCCCCGCCGGGGTGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg       <  1:523053/149‑1 (MQ=255)
                                                                                                                                             tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGCCCGCACTGCTCACCAATTTTATGCTATCCTGCCCCCGCAGTAGGGGGGCAGTATGCTAAACAGCGTACTTTCCGagcaag       >  2:698678/1‑144 (MQ=255)
                                                                                                                                              gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCAGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa      >  1:1013036/1‑149 (MQ=255)
                                                                                                                                              gAAACGACACCGCACTCTGTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCACTTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa      >  2:96037/1‑149 (MQ=255)
                                                                                                                                                  cGACCCCGCACTCTTTTTTTCTCCCTCCCCCCCCGGGGTAGTGGGCCGGGGTGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAtt  <  2:403304/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATT  >  NC_000913/3780953‑3781247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: