Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,103 A→C 16.7% intergenic (+86/‑112) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1030AC16.7% 188.0 / 2.1 108intergenic (+86/‑112)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (44/42);  new base C (10/11);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 8.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGC  >  NC_000913/3780955‑3781251
                                                                                                                                                    |                                                                                                                                                    
cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTGACCCTAAACCTCTCCCTCACGGTGAGGGGGCCGTTCGGCGGGGTATGTTCGCCCTCACTATGAAACGaca                                                                                                                                                      >  1:596079/1‑149 (MQ=255)
cAGGGGCTGGGTAAAGAGTCGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGaca                                                                                                                                                      <  1:614917/149‑1 (MQ=255)
  ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCTAACCCTCTCCCTCAGGGTGCGGGGGCCGTGCGGCGCTGTATGTAGACCCGCACTCGGAAACGGCAcc                                                                                                                                                    >  1:654056/1‑149 (MQ=255)
   gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCTCTCCCACAAGGAGAGGGGAACCGTCGGCCCTCGATGTACTCCCTGACACTGACACGACCCCg                                                                                                                                                   >  2:956210/1‑149 (MQ=255)
      cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAc                                                                                                                                                <  1:623509/149‑1 (MQ=255)
           tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                                                                                           <  1:604919/149‑1 (MQ=255)
            aatgagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                                          <  1:940014/146‑1 (MQ=255)
             aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                                         <  1:679328/149‑1 (MQ=255)
               gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGATCGGCGCTGTATGGACTACCTGACTCTGAAACGACACCGCACTAttttttt                                                                                                                                       >  2:740441/1‑149 (MQ=255)
               gagGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                                       <  1:1128839/149‑1 (MQ=255)
                agTTGCCTGAGTCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGGTGCGCCCCCTCACCCTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                                      <  2:767248/149‑1 (MQ=255)
                   tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                                   <  2:733879/149‑1 (MQ=255)
                    gCCTGCGGCACTGGCTCCCATGGCGAAAGGGTATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTccc                                                                                                                                  <  2:390188/149‑1 (MQ=255)
                     ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAGGGGGCCGTTCGGCGCGGTATGTGCTGCCTCACTCTGTTACGACACCGCCCGGGTTTTTTCTgccg                                                                                                                                 >  1:582407/1‑148 (MQ=255)
                      cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGGCCCCGAACCCACTCCCTCAGGGAGAGGGGGCCCTTCGGGGGGGTTTGTACTGCCGCACTGGGGAACGACCCCGCCCGCTTTTTTTCTcccac                                                                                                                                >  1:300753/1‑147 (MQ=255)
                       tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTACCTCAGGGATAGGGGACCGTTAGGCGCTGTATGTACTACCTCACTCTGAAACGACACCGGACTCTTTTTTTCTCCCtag                                                                                                                               >  2:730056/1‑147 (MQ=255)
                               tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGACCCCTAACCCGCTCCCACCGGGAAGGGGGCCCGTTGGGCCCGGGATGTAAGCCCGCACGAAGAAACGCACCCGCCCGCGTTTTTTCTCCCTCGCCccgccg                                                                                                                       >  2:1141145/1‑149 (MQ=255)
                                 gcgcccATGGCGAAAGGGACTGCGGCATAGTCGGTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                     <  1:255037/146‑1 (MQ=255)
                                 gCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                     <  1:698684/149‑1 (MQ=255)
                                   tCCCATGGCGAAAGGGAATGTGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                                                                   <  1:959486/149‑1 (MQ=255)
                                   tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGCGGGGGGGCCGTTCGGCGCTGTTTGTTGTCCCGCACTCTGCAACGAACCCGCCCGCGGGTTTTCTCCCTCGCCCCGCCggggg                                                                                                                   >  1:375391/1‑148 (MQ=255)
                                    cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGACCCCTAACCCTCTACCTCAGGGCGAGGGGGCCGGTCGGCGCTGGAATTCCTCCCGGACTCTGAAACGCCACCCCACTCTTTTTTTTTGCCTGGCCCCGGCggggtg                                                                                                                  >  1:1008652/1‑147 (MQ=255)
                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                 <  2:370862/149‑1 (MQ=255)
                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                 <  2:869225/149‑1 (MQ=255)
                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTACCCCAAACCCACTACCGAATGGAGCAGGGAACCTTGGGCGCAGTATTTACTACCTAACACAGAAACGACAACCCAAGCATTTTTTAACCCACGCCCCCCCGGGgaga                                                                                                                 >  2:1070661/1‑149 (MQ=255)
                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCAAACCCTAACCCTAAACCTCACGGAGCAGGGACCGGTAGGCGGGTTTTGTACTCCCTCACACTGAACCGCCCCCGCCCTGCTTTTTTCTCCCTAGCCCCTCCGGGgagc                                                                                                                 >  2:1149129/1‑148 (MQ=255)
                                      cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCGAACCCGCTCCCTCAGGGAGGGGGGCCCGGTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGGCCCCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGagag                                                                                                                >  1:57910/1‑149 (MQ=255)
                                       aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAGTCCCTGACTCTGAAACGACACCGCACGCTTTTTTTATCGCTCGCCCCTCCGGGGAGAgg                                                                                                               >  2:754569/1‑149 (MQ=255)
                                        tGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                              <  1:1151660/149‑1 (MQ=255)
                                           cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                           <  1:974771/149‑1 (MQ=255)
                                            gacaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                          <  1:739424/146‑1 (MQ=255)
                                             aaaGGGAATGCGGCATAGTGGTTTGCGCGCCCGCACCCTAACCCGCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCACTCTGACACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                         <  2:333494/149‑1 (MQ=255)
                                             aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                         <  1:407802/149‑1 (MQ=255)
                                             aaaGGGAATGCGGCATAGTCGTCTGCGCCCCCTCCCCCTCACCCTCTCCCGCCGGGAGAGGGGACCGTGCGCCGCTGTCTGTACTCCCTCACTCTGACACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                         <  1:89166/149‑1 (MQ=255)
                                                gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGGCCGTTCGGCGCTGTATGTACTCCCGCACTCGGGAACGACCCCGCCCGCGTTTTTTGTACCGCGCCCCGCCGGGGAGGGGGGCgggggg                                                                                                      >  2:525944/1‑147 (MQ=255)
                                                 ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                     <  1:759637/149‑1 (MQ=255)
                                                  gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                    <  1:199615/149‑1 (MQ=255)
                                                   actGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                   <  1:74777/147‑1 (MQ=255)
                                                   aaTGCGGCATAGTCGGTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                   <  2:262324/149‑1 (MQ=255)
                                                   aaTGCGCCATAGTCGTGTGCGCCGCCTCACCCTCCCCCTCTCCCTCCGCGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTCAACCGCCACCGCACTCTTTATTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                   <  2:290451/149‑1 (MQ=255)
                                                     tGCGGCATAGTCGTTTGCGCCCCCTCACCTTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg                                                                                                 <  2:485354/149‑1 (MQ=255)
                                                     tGCGGCATAGTCGTTTGCGCCCCCGCCCCCTAACCCTCTCCCACAGGGAGATGGGACCGTGGGGGGCGGTAGGTACGCCCTCACTCTGAAACCACACCGCACGCTTTTTTTGGCCCGAGCCCCGGCCGGGTGCGGGCCGGGGTgggagg                                                                                                 >  2:1057043/1‑144 (MQ=255)
                                                       cGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTGCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                                                                                               <  1:317337/149‑1 (MQ=255)
                                                       cGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCTCTCTGAAACCACACCGCACTCTTTTTTTCTCGCTGGCGCCTCCTGGGAGAGGGCCGGGGTGAGGGGaa                                                                                               <  1:286052/149‑1 (MQ=255)
                                                          cATAGTCGTTTGCGCCCCCTCACCCTAACCCACTCCCTCAAGGAGAGGGGACCGTTCGGCGCTGTATGTACACCCTCACTCTGAAACGCCCCCCCCCTCTTTTTTTCTCCCTCGCCCCTCCGGCGAGCGGGCCGGGGTGAGGGGAAAAg                                                                                            >  1:566433/1‑149 (MQ=255)
                                                           aTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTGTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGGCGGGGTGAGGGGAAAAgg                                                                                           >  1:831465/1‑149 (MQ=255)
                                                           aTAGTCGTTTGCGCCACCGCCCCCTACCCCTCTCCCTCCGTGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTCAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAgg                                                                                           <  1:1050268/149‑1 (MQ=255)
                                                              gtggTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCATTCTGAAACCCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCg                                                                                        <  2:743803/146‑1 (MQ=255)
                                                                     gcgcCCCCTCACCCAACCCCTCTCCCTCCGGGAGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACCACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTTAGGGGAAAAGGCCGCACTGCt                                                                                 <  1:493327/149‑1 (MQ=255)
                                                                         ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAcc                                                                             >  2:169752/1‑149 (MQ=255)
                                                                         ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                   >  1:1146062/1‑127 (MQ=255)
                                                                         ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                   <  2:1146062/127‑1 (MQ=255)
                                                                            caaaCCATAACCCTCCCCCCCAGGTTGTGTGCACCGTGCGGCGCTGTATGTACTCCCTACCTCTCAAACGACCCCTCACTCTTTTTTTCTCCCTCCCCCCTCCGGGTTGTGGGCGGGGGTTTGGGGAAAAGGCCGCACTGCTCACCAAt                                                                          <  2:483524/146‑1 (MQ=255)
                                                                            cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAAt                                                                          >  1:487284/1‑149 (MQ=255)
                                                                               aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAGTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCAAtttt                                                                       >  1:148448/1‑149 (MQ=255)
                                                                                cccTACCCCCCTCCCTCCTGGAGAGGGGACCTTCCGGCGCTGTAGGTACCCCCTCACTCTCAAACTACACCTCACTCTTTTTAACACCCACGCCCCGCCGGTTAGAGGCCCGGGGTTAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                      <  2:78927/149‑1 (MQ=255)
                                                                                 ccTAACCCTCTCCCTCAGGGAGAGGGGGCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCGCACCAATTTTCt                                                                     >  2:420843/1‑149 (MQ=255)
                                                                                 ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTATTTTTCTCCCTCGCCCCTCCGGGGAGAGGCCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                     >  2:1107465/1‑149 (MQ=255)
                                                                                 cataccccTCTCCCGCCGGGAGAGGGGACCGTGCGGCGCTGTATGAACTCCCTCCCTCTGAAACCACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGTGGGCCGGGGTGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                     <  2:72321/144‑1 (MQ=255)
                                                                                   aaaCCCTCTCCATACGGGAGTGGGAAACGTTCGTTGGTGTATGTACTCCCTCCTTCTCAAACTCCACCCCTATTTTTTTTTCTCCCTCGCCCCGCCGGGGCGTGGGCCGGGGTGTGTGTAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                                   <  2:538184/148‑1 (MQ=255)
                                                                                    tccccTCTCCCTGAGGGAGAGGGTACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAATAGGCCGCACTGCTCACCAATTTTCTGCt                                                                  >  1:554061/3‑149 (MQ=255)
                                                                                    aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                  >  1:632211/1‑149 (MQ=255)
                                                                                     ccccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGTGGATAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                                 >  2:126383/2‑149 (MQ=255)
                                                                                      cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                                >  1:477289/1‑149 (MQ=255)
                                                                                            cccTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTCCTCCCTCACTCTGAACCGCCGGCGCACGCTTTTGTTCTCCCTCGCCCGTCCGGGGAGAGGGCCGGGGTGAGGGGAGAAGGCCGCACTGGTCAGCACTTTTCTGCTTTCCTGcc                                                          >  2:175224/1‑149 (MQ=255)
                                                                                                       tgggtACCTTTCGGCGCTTTTTGAACTCCCTCATTCTTAAACCACACCGCACTCTTTTTTCCACCCTCCCCCCACCGGGGAGAGGGCCGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                               <  2:582407/148‑1 (MQ=255)
                                                                                                         gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACAAAggg                                             >  1:141820/1‑149 (MQ=255)
                                                                                                         gggACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTTTTTTAATCCCTCGCCCCGACGGGGAGAGGTCCGGGGTGAGGTGAAAAGGCCTCACTGCTCACCAATTTTCTGATATCCTCCCCCAGCACTAAgag                                             >  2:1114740/1‑147 (MQ=255)
                                                                                                         gggACCGTGCGGCGCTGTATGTACTCCCTCACTCTTAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                             <  1:539294/149‑1 (MQ=255)
                                                                                                           gACGGTTCGGCGCTGTATGCGCTCCCTCCCTCTGAACCGCCACCGCACTTTTTTTTGCTCCCTCCCCCCTCCGGGGTGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                           <  2:83280/149‑1 (MQ=255)
                                                                                                           gACCGTTCGGCGCTGTATGTACACCCTCACTCTTAAACCACCCCGCCCTCTTTTTTTCGCCCGCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                           <  2:676419/149‑1 (MQ=255)
                                                                                                            acggctCGGCGGTTTATGAACTCCCACCCTCTCAAACTCCCCCGCACTTTTTTTTTCTACCCCGCCCCTCCGGGGAGCGGGCGGGGGGGCGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                          <  2:1077986/144‑1 (MQ=255)
                                                                                                             ccGTTGGGCGCTGTAGTTACTCCCTCACTCTGAAACCACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                         <  2:831465/149‑1 (MQ=255)
                                                                                                                ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                      >  2:932047/1‑149 (MQ=255)
                                                                                                                 tCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTTCACCCGCACTTACGGGGCAGTAt                                     >  2:653414/1‑149 (MQ=255)
                                                                                                                   ggCGCTGTTTTTACTCCCTCACTCTTAACCCACACCGCACTCTTTTATTGACCCTCGCCCCTCCGGGGTGCGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                   <  1:492296/149‑1 (MQ=255)
                                                                                                                    gcgtTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                  <  1:740441/149‑1 (MQ=255)
                                                                                                                     cgcTGTATCTACTCCCTCACTCTGAAACCCCACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                 <  1:37412/149‑1 (MQ=255)
                                                                                                                       ctttatgcaCTCCCTCACTCTGAAACGCCCCACCACTCTTTTTTACACCCGCGCCCCCCGGGGGAGAGGGCGGGGGGTGGGGGAAAAGGCCGCACGGCTCACCACTTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                               <  1:78451/146‑1 (MQ=255)
                                                                                                                       cTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                               >  2:632121/1‑149 (MQ=255)
                                                                                                                            tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                          >  1:675589/1‑149 (MQ=255)
                                                                                                                             tttatCCCTCACTCTGAAACTACACCGCACTTTTTTTTTCCCCGTCGCCCCTCCGGGGAGAGGGCGGGTGTTCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                         <  2:300753/145‑1 (MQ=255)
                                                                                                                             gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTACGGGGAGAGGGCCGGGGGGCGGGGAAACGGCCGCACTGCTCACCTATTTTCTGCTATCCTGCCCCCGCGCTAAGGGGGCAGTATGCTAAACATCGt                         >  2:246087/1‑149 (MQ=255)
                                                                                                                              taCTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                        <  2:13925/149‑1 (MQ=255)
                                                                                                                              taCTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCCCCCTCGCCCCCCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                        <  1:36908/149‑1 (MQ=255)
                                                                                                                              taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                        >  1:476455/1‑149 (MQ=255)
                                                                                                                              taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                        >  2:762647/1‑149 (MQ=255)
                                                                                                                                 tCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                     >  2:568732/1‑149 (MQ=255)
                                                                                                                                 tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                     >  2:466828/1‑149 (MQ=255)
                                                                                                                                   ccTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                   >  2:287366/1‑149 (MQ=255)
                                                                                                                                    ctcacactcacaCCACCCCGCACTCTTTTTACCGCCCCCGCCCCGCCGGGTTGCGGGCGGGGGGGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                  <  1:126383/149‑1 (MQ=255)
                                                                                                                                         tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc             >  1:964427/1‑149 (MQ=255)
                                                                                                                                         tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGATCACCAATTTTCTGCTATCCTCCCCCCGCACTAAGCGGGCAGTATGCTAATCAACGTACTTTACGAAcc             >  2:626233/1‑149 (MQ=255)
                                                                                                                                         tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCCGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCCTACTTTCCGAccc             >  2:381061/1‑149 (MQ=255)
                                                                                                                                           tGAACCGACCCCCCCCTCTTTTTTCCTCCCTCCCCCCGCCGGGGTGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg           <  1:523053/149‑1 (MQ=255)
                                                                                                                                            gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCAGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa          >  1:1013036/1‑149 (MQ=255)
                                                                                                                                            gAAACGACACCGCACTCTGTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCACTTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa          >  2:96037/1‑149 (MQ=255)
                                                                                                                                                cGACCCCGCACTCTTTTTTTCTCCCTCCCCCCCCGGGGTAGTGGGCCGGGGTGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAtt      <  2:403304/149‑1 (MQ=255)
                                                                                                                                                   cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGACGCACTGCTCACAAAATTTCTGCTATCCTGCCCACGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg   >  2:648664/1‑149 (MQ=255)
                                                                                                                                                    acCTCACTCTTTTTTTCTCACTCGCCCCTCCTGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCAACAATATTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc  <  2:760652/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGC  >  NC_000913/3780955‑3781251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: