Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 260,145 G→C 5.6% intergenic (‑45/‑243) phoE ← / → proB outer membrane phosphoporin protein E/gamma‑glutamate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913260,1450GC 5.6% 297.3 / 5.7 100intergenic (‑45/‑243)phoE/proBouter membrane phosphoporin protein E/gamma‑glutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (45/48);  new base C (4/2);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

AGACATCCAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAAC  >  NC_000913/259998‑260292
                                                                                                                                                   |                                                                                                                                                   
aGACATCCAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTTCAGCTGCAGATGCCACAATGCCCAACACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGt                                                                                                                                                    >  1:686052/1‑149 (MQ=255)
      ccAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGtt                                                                                                                                              >  2:465855/1‑149 (MQ=255)
      ccAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGtt                                                                                                                                              <  1:853758/149‑1 (MQ=255)
       cAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTg                                                                                                                                             <  2:1041515/149‑1 (MQ=255)
        aGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATAAAGGCGCGAATATTCAGCGGGAGAGTCCCGTTGa                                                                                                                                            >  2:1001844/1‑149 (MQ=255)
          tttATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAACGGGACTCTCCCGCTGaat                                                                                                                                          <  1:417104/149‑2 (MQ=255)
           ttattaCCGTCTTTATTATATTTTTCTGCAGCCTGTACAGATGCAGATGCGACAATGCCCATCACCACTAATGCCAGTGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGaaaa                                                                                                                                         <  2:833143/149‑1 (MQ=255)
             attaCCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACa                                                                                                                                       <  2:593961/149‑1 (MQ=255)
             attaCCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACa                                                                                                                                       >  1:64381/1‑149 (MQ=255)
             ataaCCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGGTCTTTTTCATTTTCATTCCTGATTTTAATAAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACa                                                                                                                                       <  2:588988/146‑1 (MQ=255)
              ttaCCGTCGTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTATAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAg                                                                                                                                      <  1:374168/149‑1 (MQ=255)
               taCCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAACGGGACTCTCCCGCTGaatattcg                                                                                                                                     <  1:896905/149‑7 (MQ=255)
                 ccGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGaa                                                                                                                                   >  2:941388/1‑149 (MQ=255)
                     atttATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGtt                                                                                                                               <  2:1017629/148‑1 (MQ=255)
                      tttATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGttt                                                                                                                              <  1:1137137/149‑1 (MQ=255)
                            atatatTTCTCCAGCCTGTACAGATGCAGAAGCCACAATGCCCATCACCACTAAAGCCACACAGCTCATATACATTTACAATCCTGATAGTAAATAACTCGCGAAAATTGACCTCGATAGTCCCATTCACAACAGGAATGTATTTAAcc                                                                                                                        >  2:1053070/1‑149 (MQ=255)
                             tatatTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCt                                                                                                                       >  2:95069/1‑149 (MQ=255)
                              atatTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTg                                                                                                                      <  1:23770/149‑1 (MQ=255)
                              atatTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATACCTGATTTTAATTATCGCGCGAATATTCAGCGGGATACTCCCGTTGAAAACAGGAAAGTTTTTAAcatt                                                                                                                      >  1:1092753/1‑146 (MQ=255)
                                atTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTgag                                                                                                                    >  1:851011/1‑149 (MQ=255)
                                 tttCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAAGGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTgaga                                                                                                                   >  2:311866/1‑149 (MQ=255)
                                 tttCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGATAACAGGAAAGTTTTTACCCTgaga                                                                                                                   >  2:27273/1‑149 (MQ=255)
                                 tttCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATGTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTgaga                                                                                                                   >  1:1079494/1‑149 (MQ=255)
                                  ttCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCAAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGAt                                                                                                                  <  2:419824/149‑1 (MQ=255)
                                   tCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGAtt                                                                                                                 >  1:830277/1‑149 (MQ=255)
                                   tCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGACAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAATCTGGAAAGTTTTTAACCTGAGAtt                                                                                                                 >  2:599348/1‑149 (MQ=255)
                                    cTGCAGCCTGTACAGATGCAGATGCCACAATTCCCATCACCACTAATGCCATAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTg                                                                                                                <  1:200254/149‑1 (MQ=255)
                                    cTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTg                                                                                                                <  1:1154175/149‑1 (MQ=255)
                                     tGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTACCGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGt                                                                                                               >  1:1093201/1‑149 (MQ=255)
                                     tGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGt                                                                                                               <  1:806732/149‑1 (MQ=255)
                                      gCAGCCTGTACAGATGCAGATGACACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAAAAGGAAAGTTTTTAACCTGAGATTGtt                                                                                                              >  1:300284/1‑149 (MQ=255)
                                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTa                                                                                                             <  2:441919/149‑1 (MQ=255)
                                          ccTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAg                                                                                                          <  1:457083/149‑1 (MQ=255)
                                                  gaggcagatgcCACAATGCCCATCACCACTCATGCCAGAGTTCTCTTTTTCATTTTCAATCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGGCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTa                                                                                                  <  2:1046418/146‑1 (MQ=255)
                                                   atgcagatgcCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTAc                                                                                                 >  2:467760/1‑149 (MQ=255)
                                                   atgcagatgcCACAATGCCCATCACCACTAATGCCAGAGTGCTCATTTTCATTTTCATTCATGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTATTTAACCTGAGATTGTTAAAGATATATTAc                                                                                                 <  1:1122418/149‑1 (MQ=255)
                                                    tgcagatgcCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACa                                                                                                <  1:265649/149‑1 (MQ=255)
                                                    tgcagatgcCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACa                                                                                                <  1:278161/149‑1 (MQ=255)
                                                       agatgcCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCTTTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGACATTGTTAAAGATATATTACAGAt                                                                                             <  2:186946/149‑1 (MQ=255)
                                                             cacaATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGAGGGATAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAATATATATTACAGATtaataa                                                                                       >  2:1053428/1‑149 (MQ=255)
                                                              acaATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTTAAGATATATTACAGATTaataat                                                                                      >  2:625181/1‑149 (MQ=255)
                                                                     ccATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGATAATTTATAACCTGAGAATGTTAAAGATATATTACAGATTAATAATATTCTTa                                                                               >  2:660240/1‑149 (MQ=255)
                                                                     ccATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTa                                                                               >  2:515527/1‑149 (MQ=255)
                                                                     ccATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTa                                                                               >  1:138582/1‑149 (MQ=255)
                                                                     ccATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTa                                                                               >  1:115337/1‑149 (MQ=255)
                                                                       aTCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTaaa                                                                             >  2:342646/1‑149 (MQ=255)
                                                                          accacTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAAtg                                                                          >  2:1114047/1‑149 (MQ=255)
                                                                          accacTAATGCCAGAGTGCTCTTTTACATTTTCATTCCTGATTTTAATAAACGCGCGAATATTCAGCTGGAGATTCACTTTGATAACAGGAAACTTTTTAAACAGAGATTGTTAAAGATATAAAACAGATTAATAATAATCTTaaaaag                                                                          >  2:687757/1‑147 (MQ=255)
                                                                            cacTTATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAAtgtg                                                                        <  2:1122234/149‑1 (MQ=255)
                                                                            cacTATTGCCATTGTTCTCTTTTTCATTTTCATTCCTGTTTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTTATAATATTCTTAAAAtgtg                                                                        <  2:407947/149‑1 (MQ=255)
                                                                             acTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTgg                                                                       >  1:139365/1‑149 (MQ=255)
                                                                             acTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTAAATTAACGCGCGAATATTCAGCGGGAGAGTCACGTTGAAAACAGGAAAGATTTTAACCTCAGATAGTTAAAGATATATTACAAAATAATAAAATTCTAAAAAtgtga                                                                       >  2:479842/1‑148 (MQ=255)
                                                                             acTAATGACATAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTATCGCTCTAATATTCAGCGGGAGAGTACCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTgg                                                                       <  2:434402/149‑1 (MQ=255)
                                                                              cTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGt                                                                      >  1:167805/1‑149 (MQ=255)
                                                                              cTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACATGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGt                                                                      >  1:260598/1‑149 (MQ=255)
                                                                               tAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTa                                                                     <  1:1114047/149‑1 (MQ=255)
                                                                                aaTGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTaa                                                                    <  1:386368/149‑1 (MQ=255)
                                                                                 aTGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAAt                                                                   >  1:394583/1‑149 (MQ=255)
                                                                                 aTGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAAt                                                                   >  1:1014317/1‑149 (MQ=255)
                                                                                   gCCAGAGTGCGCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGGTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAAttt                                                                 <  2:17841/149‑1 (MQ=255)
                                                                                    ccAGAGTGCTCTTTTTGATTTTAATTCCTGATTTTAATTAACTCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATtta                                                                <  2:1037499/149‑1 (MQ=255)
                                                                                    ccAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGTAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTATAGATATATTAGATATTAATAATATTCTTAAAATGTGGTAATtta                                                                <  2:728180/149‑1 (MQ=255)
                                                                                    ccAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATtta                                                                <  1:123952/149‑1 (MQ=255)
                                                                                     cAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAATGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATttat                                                               <  1:311866/149‑1 (MQ=255)
                                                                                       gagTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATttatta                                                             <  2:300284/149‑1 (MQ=255)
                                                                                            ctctTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCt                                                        <  2:571362/149‑1 (MQ=255)
                                                                                             tctTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTg                                                       <  2:465354/149‑1 (MQ=255)
                                                                                              ctTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTTGTAATTTATTAAATCTGt                                                      >  2:199342/1‑149 (MQ=255)
                                                                                              ctTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTAAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAATTGTGGTAATTTATTAAATCTGt                                                      >  1:679913/1‑149 (MQ=255)
                                                                                                ttttcattttaaTTCCTTATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGtaa                                                    <  2:1079911/149‑1 (MQ=255)
                                                                                                 tttcattttcatTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGtaat                                                   >  1:98936/1‑149 (MQ=255)
                                                                                                     attttcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATaaaa                                               <  1:1103032/149‑1 (MQ=255)
                                                                                                      ttttcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTACATCTGTAATAAAAg                                              <  1:1148364/149‑1 (MQ=255)
                                                                                                      ttttcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAg                                              >  1:298820/1‑149 (MQ=255)
                                                                                                       tttcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAATGATATATTACAGATTAATAATTTTATTAAAATTTGGTAATTTTTTAAATCTGTATTAAAAGc                                             >  1:833053/1‑149 (MQ=255)
                                                                                                       tttcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGc                                             <  1:25714/149‑1 (MQ=255)
                                                                                                         tcattCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGt                                           <  1:706095/149‑1 (MQ=255)
                                                                                                            ttCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTaaa                                        <  1:185572/149‑1 (MQ=255)
                                                                                                            ttCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTaaa                                        <  1:926859/149‑1 (MQ=255)
                                                                                                              ccTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAaaca                                      >  2:1127534/1‑149 (MQ=255)
                                                                                                               cTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAaacaa                                     >  2:222193/1‑149 (MQ=255)
                                                                                                               cTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAaacaa                                     >  1:1106986/1‑149 (MQ=255)
                                                                                                                 gATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACt                                   >  2:877158/1‑149 (MQ=255)
                                                                                                                      taattaaCGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGc                              <  2:64381/149‑1 (MQ=255)
                                                                                                                        attaaCGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTa                            >  1:461800/1‑149 (MQ=255)
                                                                                                                              gcgcgAATATTCAGCGGGAGAGTCCCTTTGAAATCAGGAATGTTTTTAACCTGAGATTTTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAAAAAAAGCGTAAACATCTGCCGCTAGGCTTg                      <  1:944604/149‑1 (MQ=255)
                                                                                                                                gcgAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTTAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCt                    <  2:710436/149‑1 (MQ=255)
                                                                                                                                gcgAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCt                    <  1:465855/149‑1 (MQ=255)
                                                                                                                                 cctgttttCAACGGGACTCTCCCGCTGAATACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATTTATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCAGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTg                   >  1:288712/7‑149 (MQ=255)
                                                                                                                                 cctgttttCAACGGGACTCTCCCGCTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTg                   >  1:1038799/7‑149 (MQ=255)
                                                                                                                                  gAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGa                  >  2:269308/1‑149 (MQ=255)
                                                                                                                                  gAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCCGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGa                  >  2:460245/1‑149 (MQ=255)
                                                                                                                                   aaTATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCGGTAATAAAAGCGTAAACAACTGCCGCTAGGCTAGCTGAt                 <  1:701132/149‑1 (MQ=255)
                                                                                                                                    atatTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATc                <  2:1145991/149‑1 (MQ=255)
                                                                                                                                     tatTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATcc               <  2:456083/149‑1 (MQ=255)
                                                                                                                                      atTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATccc              <  2:851011/149‑1 (MQ=255)
                                                                                                                                        tCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCcgc            >  1:905150/1‑149 (MQ=255)
                                                                                                                                          aGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCgcgc          >  1:207459/1‑149 (MQ=255)
                                                                                                                                          aGCGGGAGACTCCCGTTGAAAACAGTAAAGTTTTTAACCTGAGATTGATAAAGATAAATTACAGATAAATAATATTCATAAAATGTGTTAATTTATTAAAACTGTAATAAAATCGTAAACAACTGCCGCTAGGCTTGATAATCCCccgc          >  2:79763/1‑149 (MQ=255)
                                                                                                                                                  agTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAAc  <  1:467760/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGACATCCAGTTTATTACCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGGGAGAGTCCCGTTGAAAACAGGAAAGTTTTTAACCTGAGATTGTTAAAGATATATTACAGATTAATAATATTCTTAAAATGTGGTAATTTATTAAATCTGTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAAC  >  NC_000913/259998‑260292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: