Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,428 C→G 6.0% intergenic (‑62/+121) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,4280CG 6.0% 179.1 / 4.7 67intergenic (‑62/+121)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (23/38);  new base G (2/2);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

GATGTTTTTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCG  >  NC_000913/3908280‑3908568
                                                                                                                                                    |                                                                                                                                            
gATGTTTTTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATc                                                                                                                                              <  2:621892/149‑1 (MQ=255)
gATGTTTTTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATc                                                                                                                                              >  1:291641/1‑149 (MQ=255)
 aTGTTTTTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCa                                                                                                                                             <  2:206730/149‑1 (MQ=255)
    tttttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGc                                                                                                                                          <  1:1005920/149‑1 (MQ=255)
    tttttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGc                                                                                                                                          >  1:728657/1‑149 (MQ=255)
       ttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGtt                                                                                                                                       <  1:900640/149‑1 (MQ=255)
       ttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGAAGGCCGGATCAGGCGtt                                                                                                                                       >  2:655718/1‑149 (MQ=255)
          aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                    <  2:1165886/149‑1 (MQ=255)
          aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACACATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                    >  1:1018105/1‑149 (MQ=255)
           gggCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcg                                                                                                                                   <  2:71078/149‑1 (MQ=255)
              cATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGTCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                >  1:944245/1‑149 (MQ=255)
                  gAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATc                                                                                                                            <  1:1079664/149‑1 (MQ=255)
                    aTTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                          <  1:248016/149‑1 (MQ=255)
                    aTTTCCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCg                                                                                                                          <  2:688334/149‑1 (MQ=255)
                       tgacGTAGTAGAAGTTCAAATTACGAACATGAATTTTACTCGGGGGAGTTTCAACCATACTCAATGCAATCTTTGCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCGGcc                                                                                                                       <  1:655942/146‑2 (MQ=255)
                        gCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGGCGCATCCGGCaa                                                                                                                      >  2:45748/1‑149 (MQ=255)
                         ccGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaaa                                                                                                                     <  1:141836/149‑1 (MQ=255)
                          cGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAg                                                                                                                    <  2:842750/149‑1 (MQ=255)
                           gtagtaGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCGGGCAAAgt                                                                                                                   >  1:560286/1‑149 (MQ=255)
                            tagtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCATAATCGTTCATGATTAACTTCGCTAATCAGGATTACCGTATGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                  <  2:70108/149‑1 (MQ=255)
                            tagtagAAGTTCAAATTACGAACCTGAATTTAACTCGGGGCAGTATCAACCCTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                  <  1:1120145/149‑1 (MQ=255)
                            tagtagAAGTTCAAAATACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                  <  1:483782/149‑1 (MQ=255)
                              gtagAAGATCAAATTACGAACCTGAATTTTACTCGGGGCAGTCTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTACGCCGGATCAGGCGTTCACTCCGCATCCGGCAAAGTGtt                                                                                                                <  2:281830/149‑1 (MQ=255)
                                    gTTCAAATAACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgt                                                                                                          <  1:808042/149‑1 (MQ=255)
                                       cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTa                                                                                                       >  1:585146/1‑149 (MQ=255)
                                            tACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACagag                                                                                                  <  1:38863/149‑1 (MQ=255)
                                             aCGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGt                                                                                                 >  1:1076433/1‑149 (MQ=255)
                                              cGAACCTGAATTTTACTCGGGTCAGTTTCTACCTTTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                <  2:1006191/149‑1 (MQ=255)
                                              cGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                <  1:1126642/149‑1 (MQ=255)
                                               gAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCa                                                                                               >  1:559025/1‑149 (MQ=255)
                                                   atGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCATTCTTTTCCTCATCTTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCGGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCaataa                                                                                           <  1:258826/148‑1 (MQ=255)
                                                    tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAg                                                                                          >  2:838861/1‑149 (MQ=255)
                                                            aCTCGGGGCAGGTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcgg                                                                                  <  1:675309/149‑1 (MQ=255)
                                                              tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATGCGGCAAAGTGTTAACCGTGGACAGAGTCAAAACGGCGCtcggtc                                                                                >  2:1037715/1‑149 (MQ=255)
                                                              tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTAACGCCGCATCCGGCAAAGTGTTAACCCTGTACAGAGTAAAAAAGGCGCtcggtc                                                                                >  2:1007560/1‑149 (MQ=255)
                                                               cGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                               <  2:728657/149‑1 (MQ=255)
                                                                         tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGc                                                                     >  2:758527/1‑149 (MQ=255)
                                                                          cAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGcc                                                                    >  1:851258/1‑149 (MQ=255)
                                                                             ccATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCct                                                                 >  1:684620/1‑149 (MQ=255)
                                                                                 aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATGAGGCTTACCGTAGGCCGGATCAGTCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                             <  2:588970/149‑1 (MQ=255)
                                                                                  cTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                            <  1:220022/149‑1 (MQ=255)
                                                                                  cTCATTGCAAGCTTTACCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                            <  2:201352/149‑1 (MQ=255)
                                                                                  atCATTGCAATCTTTTCCTCCTCCGGCCTGATGCACATCCCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                            <  2:250825/148‑1 (MQ=39)
                                                                                   tCATTGCAATCTTTTCCTCATCGTGCCTGCTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                           >  2:879017/1‑149 (MQ=255)
                                                                                     aTTGCAATCTTTTCCTCATCGTGCCTGATCCACTTCGATAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCCCGCCGCATACGGCAAAGTGTTACCCGTGTACAGAGTCTAAAAGGAGAACGGTCGGTCCCCTCGCCCCTCTGGTgag                                                         >  2:1048714/1‑149 (MQ=255)
                                                                                       tGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGagag                                                       <  1:412309/149‑1 (MQ=255)
                                                                                          aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                    >  1:689199/1‑149 (MQ=255)
                                                                                           aTCGTTTCCTCATCGGGCCTGATCCACTTCGCAAATCAGGCTTACCGGAGGCCGGAGCAGGCGCTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCGCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgggga                                                   <  1:665867/149‑3 (MQ=255)
                                                                                              ttttCCTCCTCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGAACAGAGTCAAAAAGGCGCTCGGTCGGACCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                <  2:900162/149‑1 (MQ=255)
                                                                                              ttttCCTCATCGTGACTGCTGCACTTCGCTACTCAGCCATACCGTAGGCCGGATCAGGCGTTCACGCCGCAAGCGGCAAAGTGTAAACCGTGAACAGAGTCAAAAATGCGCTCGGTCGGGCCCCTAGCCCCTCTGGGGAGAGGGTTAgg                                                <  1:801114/149‑1 (MQ=255)
                                                                                                   cTCATCGTGCCTGATGCACTTCGCTAAGCAGGCTTACCGTAGGCCGGATCAGGAGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                           <  2:863891/149‑1 (MQ=255)
                                                                                                    tCATCGTGCCTGATGCACTTCGCTAATCATGCGTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAgg                                          <  1:1072217/149‑1 (MQ=39)
                                                                                                    tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                          <  2:57844/149‑1 (MQ=255)
                                                                                                      aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGAAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                        <  2:903409/149‑1 (MQ=255)
                                                                                                       tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCTGTCCCCTCGACCCTCTGTGGAGAGGGTTAGTGGGAgtggt                                       >  2:1144189/1‑148 (MQ=38)
                                                                                                            ccTGATGCACTTCGCTAATCAGGCTTACCGCAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTTCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                  <  1:655718/149‑1 (MQ=255)
                                                                                                              tGATCCAGTTCGCAAATCAGGCTTACCGTAGGCCGGATCTGGCGTGCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCCCTGGGGCGAGGTTTAGGGTGAGGGGAAAACCgt                                <  2:172896/149‑1 (MQ=255)
                                                                                                                aTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtgt                              <  1:1160083/149‑1 (MQ=255)
                                                                                                                   cACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGATGGTTAGGGTGAGGGGAAAACAGTGTCAg                           >  1:1112922/1‑149 (MQ=255)
                                                                                                                            aaTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCTTGGGGCAAAGTGTAAACGGTGTACAGAGCCAAAACGGCCGGCGGGGGGTCCCCTCGCCCCTGTGGGGTGTGGGTTAGGGTGAGGGGATAACCGTGTCAGCAATATCaa                  <  2:1018105/149‑1 (MQ=255)
                                                                                                                            aaTCAGGCTTACCCTAGGCCGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAACGCGCTCGTTCGGTCCCCTAGCCCCTCTGGGGAGAGGGTTAGGGTTAGGGGAAAACCGAGTAAGCAATATCaa                  >  1:443654/1‑149 (MQ=38)
                                                                                                                             aTCCGGCTTACCGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCAAAGTGTTAACCGTTTACAGAGTCAAAACGGCGCTGGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                 <  2:383117/149‑1 (MQ=38)
                                                                                                                                    ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGGTCAGCAATATCAACCGTGttt          >  1:238563/1‑149 (MQ=255)
                                                                                                                                    ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCACTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCTTGttt          >  2:967523/1‑149 (MQ=255)
                                                                                                                                     caCCGTAGGCCGTACCAGGCGTTCTCGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCCCCCCGCTGGGGAGAGGGTTAGGGTGAGGGGTAAACCGTGTCAGCAATATCAACCGTGTTTa         <  2:1167787/148‑1 (MQ=38)
                                                                                                                                        cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc      >  1:558964/1‑149 (MQ=255)
                                                                                                                                          tttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt    >  1:492316/4‑149 (MQ=38)
                                                                                                                                           ttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc   >  2:268516/3‑149 (MQ=38)
                                                                                                                                            ggCCGTATCAGGCGTTCCCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGGGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg  <  1:1063571/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GATGTTTTTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCG  >  NC_000913/3908280‑3908568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: