Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,435 T→G 7.1% intergenic (‑69/+114) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,4350TG 7.1% 151.3 / 2.7 65intergenic (‑69/+114)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (23/37);  new base G (2/3);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

TTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAA  >  NC_000913/3908287‑3908577
                                                                                                                                                    |                                                                                                                                              
ttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGtt                                                                                                                                                <  1:900640/149‑1 (MQ=255)
ttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGAAGGCCGGATCAGGCGtt                                                                                                                                                >  2:655718/1‑149 (MQ=255)
   aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                             <  2:1165886/149‑1 (MQ=255)
   aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACACATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                             >  1:1018105/1‑149 (MQ=255)
    gggCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcg                                                                                                                                            <  2:71078/149‑1 (MQ=255)
       cATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGTCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                         >  1:944245/1‑149 (MQ=255)
           gAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATc                                                                                                                                     <  1:1079664/149‑1 (MQ=255)
             aTTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                                   <  1:248016/149‑1 (MQ=255)
             aTTTCCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCg                                                                                                                                   <  2:688334/149‑1 (MQ=255)
                tgacGTAGTAGAAGTTCAAATTACGAACATGAATTTTACTCGGGGGAGTTTCAACCATACTCAATGCAATCTTTGCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCGGcc                                                                                                                                <  1:655942/146‑2 (MQ=255)
                 gCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGGCGCATCCGGCaa                                                                                                                               >  2:45748/1‑149 (MQ=255)
                  ccGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaaa                                                                                                                              <  1:141836/149‑1 (MQ=255)
                   cGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAg                                                                                                                             <  2:842750/149‑1 (MQ=255)
                    gtagtaGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCGGGCAAAgt                                                                                                                            >  1:560286/1‑149 (MQ=255)
                     tagtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCATAATCGTTCATGATTAACTTCGCTAATCAGGATTACCGTATGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                           <  2:70108/149‑1 (MQ=255)
                     tagtagAAGTTCAAATTACGAACCTGAATTTAACTCGGGGCAGTATCAACCCTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                           <  1:1120145/149‑1 (MQ=255)
                     tagtagAAGTTCAAAATACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                           <  1:483782/149‑1 (MQ=255)
                       gtagAAGATCAAATTACGAACCTGAATTTTACTCGGGGCAGTCTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTACGCCGGATCAGGCGTTCACTCCGCATCCGGCAAAGTGtt                                                                                                                         <  2:281830/149‑1 (MQ=255)
                             gTTCAAATAACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgt                                                                                                                   <  1:808042/149‑1 (MQ=255)
                                cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTa                                                                                                                >  1:585146/1‑149 (MQ=255)
                                     tACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACagag                                                                                                           <  1:38863/149‑1 (MQ=255)
                                      aCGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGt                                                                                                          >  1:1076433/1‑149 (MQ=255)
                                       cGAACCTGAATTTTACTCGGGTCAGTTTCTACCTTTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                         <  2:1006191/149‑1 (MQ=255)
                                       cGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                         <  1:1126642/149‑1 (MQ=255)
                                        gAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCa                                                                                                        >  1:559025/1‑149 (MQ=255)
                                            atGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCATTCTTTTCCTCATCTTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCGGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCaataa                                                                                                    <  1:258826/148‑1 (MQ=255)
                                             tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAg                                                                                                   >  2:838861/1‑149 (MQ=255)
                                                     aCTCGGGGCAGGTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcgg                                                                                           <  1:675309/149‑1 (MQ=255)
                                                       tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATGCGGCAAAGTGTTAACCGTGGACAGAGTCAAAACGGCGCtcggtc                                                                                         >  2:1037715/1‑149 (MQ=255)
                                                       tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTAACGCCGCATCCGGCAAAGTGTTAACCCTGTACAGAGTAAAAAAGGCGCtcggtc                                                                                         >  2:1007560/1‑149 (MQ=255)
                                                        cGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                        <  2:728657/149‑1 (MQ=255)
                                                                  tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGc                                                                              >  2:758527/1‑149 (MQ=255)
                                                                   cAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGcc                                                                             >  1:851258/1‑149 (MQ=255)
                                                                      ccATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCct                                                                          >  1:684620/1‑149 (MQ=255)
                                                                          aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATGAGGCTTACCGTAGGCCGGATCAGTCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                      <  2:588970/149‑1 (MQ=255)
                                                                           cTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                     <  1:220022/149‑1 (MQ=255)
                                                                           cTCATTGCAAGCTTTACCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                     <  2:201352/149‑1 (MQ=255)
                                                                           atCATTGCAATCTTTTCCTCCTCCGGCCTGATGCACATCCCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                     <  2:250825/148‑1 (MQ=39)
                                                                            tCATTGCAATCTTTTCCTCATCGTGCCTGCTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                    >  2:879017/1‑149 (MQ=255)
                                                                              aTTGCAATCTTTTCCTCATCGTGCCTGATCCACTTCGATAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCCCGCCGCATACGGCAAAGTGTTACCCGTGTACAGAGTCTAAAAGGAGAACGGTCGGTCCCCTCGCCCCTCTGGTgag                                                                  >  2:1048714/1‑149 (MQ=255)
                                                                                tGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGagag                                                                <  1:412309/149‑1 (MQ=255)
                                                                                   aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                             >  1:689199/1‑149 (MQ=255)
                                                                                    aTCGTTTCCTCATCGGGCCTGATCCACTTCGCAAATCAGGCTTACCGGAGGCCGGAGCAGGCGCTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCGCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgggga                                                            <  1:665867/149‑3 (MQ=255)
                                                                                       ttttCCTCCTCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGAACAGAGTCAAAAAGGCGCTCGGTCGGACCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                         <  2:900162/149‑1 (MQ=255)
                                                                                       ttttCCTCATCGTGACTGCTGCACTTCGCTACTCAGCCATACCGTAGGCCGGATCAGGCGTTCACGCCGCAAGCGGCAAAGTGTAAACCGTGAACAGAGTCAAAAATGCGCTCGGTCGGGCCCCTAGCCCCTCTGGGGAGAGGGTTAgg                                                         <  1:801114/149‑1 (MQ=255)
                                                                                            cTCATCGTGCCTGATGCACTTCGCTAAGCAGGCTTACCGTAGGCCGGATCAGGAGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                    <  2:863891/149‑1 (MQ=255)
                                                                                             tCATCGTGCCTGATGCACTTCGCTAATCATGCGTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAgg                                                   <  1:1072217/149‑1 (MQ=39)
                                                                                             tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                   <  2:57844/149‑1 (MQ=255)
                                                                                               aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGAAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                 <  2:903409/149‑1 (MQ=255)
                                                                                                tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCTGTCCCCTCGACCCTCTGTGGAGAGGGTTAGTGGGAgtggt                                                >  2:1144189/1‑148 (MQ=38)
                                                                                                     ccTGATGCACTTCGCTAATCAGGCTTACCGCAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTTCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                           <  1:655718/149‑1 (MQ=255)
                                                                                                       tGATCCAGTTCGCAAATCAGGCTTACCGTAGGCCGGATCTGGCGTGCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCCCTGGGGCGAGGTTTAGGGTGAGGGGAAAACCgt                                         <  2:172896/149‑1 (MQ=255)
                                                                                                         aTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtgt                                       <  1:1160083/149‑1 (MQ=255)
                                                                                                            cACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGATGGTTAGGGTGAGGGGAAAACAGTGTCAg                                    >  1:1112922/1‑149 (MQ=255)
                                                                                                                     aaTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCTTGGGGCAAAGTGTAAACGGTGTACAGAGCCAAAACGGCCGGCGGGGGGTCCCCTCGCCCCTGTGGGGTGTGGGTTAGGGTGAGGGGATAACCGTGTCAGCAATATCaa                           <  2:1018105/149‑1 (MQ=255)
                                                                                                                     aaTCAGGCTTACCCTAGGCCGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAACGCGCTCGTTCGGTCCCCTAGCCCCTCTGGGGAGAGGGTTAGGGTTAGGGGAAAACCGAGTAAGCAATATCaa                           >  1:443654/1‑149 (MQ=38)
                                                                                                                      aTCCGGCTTACCGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCAAAGTGTTAACCGTTTACAGAGTCAAAACGGCGCTGGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                          <  2:383117/149‑1 (MQ=38)
                                                                                                                             ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGGTCAGCAATATCAACCGTGttt                   >  1:238563/1‑149 (MQ=255)
                                                                                                                             ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCACTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCTTGttt                   >  2:967523/1‑149 (MQ=255)
                                                                                                                              caCCGTAGGCCGTACCAGGCGTTCTCGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCCCCCCGCTGGGGAGAGGGTTAGGGTGAGGGGTAAACCGTGTCAGCAATATCAACCGTGTTTa                  <  2:1167787/148‑1 (MQ=38)
                                                                                                                                 cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc               >  1:558964/1‑149 (MQ=255)
                                                                                                                                   tttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt             >  1:492316/4‑149 (MQ=38)
                                                                                                                                    ttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc            >  2:268516/3‑149 (MQ=38)
                                                                                                                                     ggCCGTATCAGGCGTTCCCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGGGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg           <  1:1063571/149‑1 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTCTGTCCCCTCGCCCCTCTGGGGAGAGGGTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa  <  2:944245/149‑1 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa  <  1:758527/149‑1 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCACCTCTGGGGAGAGGGTTAGGGTGATGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCTCAAAaacaa  >  2:734382/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAA  >  NC_000913/3908287‑3908577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: