Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,443 C→G 6.6% intergenic (‑77/+106) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,4430CG 6.6% 167.3 / 4.9 61intergenic (‑77/+106)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (21/36);  new base G (2/2);  total (23/38)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

GAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAG  >  NC_000913/3908298‑3908587
                                                                                                                                                 |                                                                                                                                                
gAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATc                                                                                                                                               <  1:1079664/149‑1 (MQ=255)
  aTTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCg                                                                                                                                             <  1:248016/149‑1 (MQ=255)
  aTTTCCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCg                                                                                                                                             <  2:688334/149‑1 (MQ=255)
     tgacGTAGTAGAAGTTCAAATTACGAACATGAATTTTACTCGGGGGAGTTTCAACCATACTCAATGCAATCTTTGCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCGGcc                                                                                                                                          <  1:655942/146‑2 (MQ=255)
      gCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGGCGCATCCGGCaa                                                                                                                                         >  2:45748/1‑149 (MQ=255)
       ccGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaaa                                                                                                                                        <  1:141836/149‑1 (MQ=255)
        cGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAg                                                                                                                                       <  2:842750/149‑1 (MQ=255)
         gtagtaGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCGGGCAAAgt                                                                                                                                      >  1:560286/1‑149 (MQ=255)
          tagtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCATAATCGTTCATGATTAACTTCGCTAATCAGGATTACCGTATGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                                     <  2:70108/149‑1 (MQ=255)
          tagtagAAGTTCAAATTACGAACCTGAATTTAACTCGGGGCAGTATCAACCCTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                                     <  1:1120145/149‑1 (MQ=255)
          tagtagAAGTTCAAAATACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                                     <  1:483782/149‑1 (MQ=255)
            gtagAAGATCAAATTACGAACCTGAATTTTACTCGGGGCAGTCTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTACGCCGGATCAGGCGTTCACTCCGCATCCGGCAAAGTGtt                                                                                                                                   <  2:281830/149‑1 (MQ=255)
                  gTTCAAATAACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgt                                                                                                                             <  1:808042/149‑1 (MQ=255)
                     cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTa                                                                                                                          >  1:585146/1‑149 (MQ=255)
                          tACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACagag                                                                                                                     <  1:38863/149‑1 (MQ=255)
                           aCGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGt                                                                                                                    >  1:1076433/1‑149 (MQ=255)
                            cGAACCTGAATTTTACTCGGGTCAGTTTCTACCTTTCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                                   <  2:1006191/149‑1 (MQ=255)
                            cGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTc                                                                                                                   <  1:1126642/149‑1 (MQ=255)
                             gAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCa                                                                                                                  >  1:559025/1‑149 (MQ=255)
                                 atGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCATTCTTTTCCTCATCTTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCGGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCaataa                                                                                                              <  1:258826/148‑1 (MQ=255)
                                  tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAg                                                                                                             >  2:838861/1‑149 (MQ=255)
                                          aCTCGGGGCAGGTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcgg                                                                                                     <  1:675309/149‑1 (MQ=255)
                                            tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATGCGGCAAAGTGTTAACCGTGGACAGAGTCAAAACGGCGCtcggtc                                                                                                   >  2:1037715/1‑149 (MQ=255)
                                            tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTAACGCCGCATCCGGCAAAGTGTTAACCCTGTACAGAGTAAAAAAGGCGCtcggtc                                                                                                   >  2:1007560/1‑149 (MQ=255)
                                             cGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                                  <  2:728657/149‑1 (MQ=255)
                                                       tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGc                                                                                        >  2:758527/1‑149 (MQ=255)
                                                        cAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGcc                                                                                       >  1:851258/1‑149 (MQ=255)
                                                           ccATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCct                                                                                    >  1:684620/1‑149 (MQ=255)
                                                               aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATGAGGCTTACCGTAGGCCGGATCAGTCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                <  2:588970/149‑1 (MQ=255)
                                                                cTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                               <  1:220022/149‑1 (MQ=255)
                                                                cTCATTGCAAGCTTTACCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                               <  2:201352/149‑1 (MQ=255)
                                                                atCATTGCAATCTTTTCCTCCTCCGGCCTGATGCACATCCCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                               <  2:250825/148‑1 (MQ=39)
                                                                 tCATTGCAATCTTTTCCTCATCGTGCCTGCTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                              >  2:879017/1‑149 (MQ=255)
                                                                   aTTGCAATCTTTTCCTCATCGTGCCTGATCCACTTCGATAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCCCGCCGCATACGGCAAAGTGTTACCCGTGTACAGAGTCTAAAAGGAGAACGGTCGGTCCCCTCGCCCCTCTGGTgag                                                                            >  2:1048714/1‑149 (MQ=255)
                                                                     tGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGagag                                                                          <  1:412309/149‑1 (MQ=255)
                                                                        aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                       >  1:689199/1‑149 (MQ=255)
                                                                         aTCGTTTCCTCATCGGGCCTGATCCACTTCGCAAATCAGGCTTACCGGAGGCCGGAGCAGGCGCTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCGCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgggga                                                                      <  1:665867/149‑3 (MQ=255)
                                                                            ttttCCTCCTCGTGCCTGATGCACTTCGCTACTCAGGCTTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGAACAGAGTCAAAAAGGCGCTCGGTCGGACCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                   <  2:900162/149‑1 (MQ=255)
                                                                            ttttCCTCATCGTGACTGCTGCACTTCGCTACTCAGCCATACCGTAGGCCGGATCAGGCGTTCACGCCGCAAGCGGCAAAGTGTAAACCGTGAACAGAGTCAAAAATGCGCTCGGTCGGGCCCCTAGCCCCTCTGGGGAGAGGGTTAgg                                                                   <  1:801114/149‑1 (MQ=255)
                                                                                 cTCATCGTGCCTGATGCACTTCGCTAAGCAGGCTTACCGTAGGCCGGATCAGGAGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                              <  2:863891/149‑1 (MQ=255)
                                                                                  tCATCGTGCCTGATGCACTTCGCTAATCATGCGTACCGTAGTCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAgg                                                             <  1:1072217/149‑1 (MQ=39)
                                                                                  tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                             <  2:57844/149‑1 (MQ=255)
                                                                                    aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGAAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                           <  2:903409/149‑1 (MQ=255)
                                                                                     tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCTGTCCCCTCGACCCTCTGTGGAGAGGGTTAGTGGGAgtggt                                                          >  2:1144189/1‑148 (MQ=38)
                                                                                          ccTGATGCACTTCGCTAATCAGGCTTACCGCAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTTCAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                                     <  1:655718/149‑1 (MQ=255)
                                                                                            tGATCCAGTTCGCAAATCAGGCTTACCGTAGGCCGGATCTGGCGTGCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCCCTGGGGCGAGGTTTAGGGTGAGGGGAAAACCgt                                                   <  2:172896/149‑1 (MQ=255)
                                                                                              aTGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGGTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtgt                                                 <  1:1160083/149‑1 (MQ=255)
                                                                                                 cACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGATGGTTAGGGTGAGGGGAAAACAGTGTCAg                                              >  1:1112922/1‑149 (MQ=255)
                                                                                                          aaTCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCTTGGGGCAAAGTGTAAACGGTGTACAGAGCCAAAACGGCCGGCGGGGGGTCCCCTCGCCCCTGTGGGGTGTGGGTTAGGGTGAGGGGATAACCGTGTCAGCAATATCaa                                     <  2:1018105/149‑1 (MQ=255)
                                                                                                          aaTCAGGCTTACCCTAGGCCGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAACGCGCTCGTTCGGTCCCCTAGCCCCTCTGGGGAGAGGGTTAGGGTTAGGGGAAAACCGAGTAAGCAATATCaa                                     >  1:443654/1‑149 (MQ=38)
                                                                                                           aTCCGGCTTACCGTAGGCCGGATAAGGCGATCACGCCGCATCCGGCAAAGTGTTAACCGTTTACAGAGTCAAAACGGCGCTGGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAAc                                    <  2:383117/149‑1 (MQ=38)
                                                                                                                  ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGGGTCAGCAATATCAACCGTGttt                             >  1:238563/1‑149 (MQ=255)
                                                                                                                  ttACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCACTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCTTGttt                             >  2:967523/1‑149 (MQ=255)
                                                                                                                   caCCGTAGGCCGTACCAGGCGTTCTCGCCGCATCCGGCAAAGTGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGGCGGTCCCCTCCCCCCGCTGGGGAGAGGGTTAGGGTGAGGGGTAAACCGTGTCAGCAATATCAACCGTGTTTa                            <  2:1167787/148‑1 (MQ=38)
                                                                                                                      cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttc                         >  1:558964/1‑149 (MQ=255)
                                                                                                                        tttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt                       >  1:492316/4‑149 (MQ=38)
                                                                                                                         ttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                      >  2:268516/3‑149 (MQ=38)
                                                                                                                          ggCCGTATCAGGCGTTCCCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGGGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg                     <  1:1063571/149‑1 (MQ=255)
                                                                                                                                   aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTCTGTCCCCTCGCCCCTCTGGGGAGAGGGTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa            <  2:944245/149‑1 (MQ=255)
                                                                                                                                   aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa            <  1:758527/149‑1 (MQ=255)
                                                                                                                                   aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCACCTCTGGGGAGAGGGTTAGGGTGATGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCTCAAAaacaa            >  2:734382/1‑149 (MQ=255)
                                                                                                                                             gccgcATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCGCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGGCAGCAAGATCAACCGTGTTTAGTCTTCGCACAAACACCGCGCGCGAg  >  2:745118/1‑149 (MQ=38)
                                                                                                                                             cccgcATCCGGCAAAGTGTTAAACGTGTACAGAGTCAAAAAGGCTCTCGGTCGGTCCCGTCGCCCCTCTGGGGAGAGGGGTAGGGGGAGCGGAAAACCGTGTCAGCGATATCAACAGTGATTATACTTCGCAAAAACAACACGCGCCAg  >  2:695164/2‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAG  >  NC_000913/3908298‑3908587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑