Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,023,526 T→G 7.5% intergenic (+14/‑28) tatC → / → tatD TatABCE protein translocation system subunit/quality control of Tat‑exported FeS proteins; Mg‑dependent cytoplasmic DNase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,023,5260TG 7.5% 248.8 / 5.5 110intergenic (+14/‑28)tatC/tatDTatABCE protein translocation system subunit/quality control of Tat‑exported FeS proteins; Mg‑dependent cytoplasmic DNase
Reads supporting (aligned to +/- strand):  ref base T (54/46);  new base G (3/6);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 110 total aligned reads displayed.

AACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCGT  >  NC_000913/4023380‑4023673
                                                                                                                                                  |                                                                                                                                                   
aaCGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCCCCCGTCa                                                                                                                                                   >  2:82568/1‑149 (MQ=255)
  cGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACTCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAgg                                                                                                                                                 >  1:974530/1‑149 (MQ=255)
  cGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAgg                                                                                                                                                 >  2:925170/1‑149 (MQ=255)
   gCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                                                                                                                >  1:291287/1‑149 (MQ=255)
      ggtGGCGATCGCGATGTACTGTCTGTATGACATCGGTGTCTTCTTCTAACGCTTTTTCGTTGGTAACGGGCGATAACGGCAAGAGGAATACGTCGCTGAAGCAGAAAGCGAATAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCgg                                                                                                                                             <  1:699809/147‑1 (MQ=255)
        tGGCGATCCCTATGTACTGTCTGTTTGATATCGGTGTCTTCTTCTCACGCTTTTACGTTTGTAAAGGGCGAAATCGGGAAGAGGAATACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGtt                                                                                                                                           <  1:788345/149‑1 (MQ=255)
        tGGCGATCCCGATGTACTGTCTGTTTGAACTCGGTGTCTTCTTCTCACGCTTTTACGGTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCTGACGGGCGGtt                                                                                                                                           <  1:1064596/149‑1 (MQ=255)
        gggCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCCCGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGACCCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGtt                                                                                                                                           <  1:1041727/148‑1 (MQ=255)
          gCGATCCCGATGTACTGTCTGTTTGTAATCGGGGTCTTCTTCTCACGCTTTTACGTTTGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGt                                                                                                                                         <  2:1073533/149‑1 (MQ=255)
          gCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTATACATTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCTGACGGGCGGTTga                                                                                                                                         <  2:913696/149‑2 (MQ=255)
             ataccGATGTACTGTCTGTTTGAAATCCGTGTCTTCTTCGCACGCTTTTCCGTTGGTACAGGGCGAAATCGTGAAGAGGACAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGGCAGGGCGGTTGTCat                                                                                                                                      <  2:245641/146‑1 (MQ=255)
             aTCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCat                                                                                                                                      >  1:1093603/1‑149 (MQ=255)
              tCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCata                                                                                                                                     <  2:304627/149‑1 (MQ=255)
                  gATGTACTGTATGGTTGACATCGGTGTCTTCTTCCCACGCTTTTACGTTTGAAAATGGCGAATTCGGGATGAGGACAAGGACGCTGAAGAAGACAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATCTGGa                                                                                                                                 <  2:840135/149‑1 (MQ=255)
                   aTGTACTGTCTGTTTGAAATCGGGGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAATCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAg                                                                                                                                <  1:759815/149‑1 (MQ=255)
                     gTACTGTCTGTTTGACATCGGGGTCTTCTTCTCACGCGTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTCAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTa                                                                                                                              <  1:112708/149‑1 (MQ=255)
                      tACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGATATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGGCAGGGCGGTTGTCTTATGGAGTAc                                                                                                                             >  2:228905/1‑149 (MQ=255)
                      tACTGTCTGTTTGAAATCGGTGTCTTCTTCTCAAGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTAc                                                                                                                             >  1:903124/1‑149 (MQ=255)
                       aCTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAATCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACa                                                                                                                            <  2:1122302/149‑1 (MQ=255)
                       aCTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACa                                                                                                                            >  2:583394/1‑149 (MQ=255)
                        ctgtctttTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCTAAATCGGTAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAg                                                                                                                           <  1:157861/149‑1 (MQ=255)
                        ctgtctgtTTGAAATCGGTGTCTTCTTCTCACGCTATTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAg                                                                                                                           <  2:887734/149‑1 (MQ=255)
                             tgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAACCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGt                                                                                                                      >  1:645951/1‑149 (MQ=255)
                              gtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGtt                                                                                                                     >  2:214301/1‑149 (MQ=255)
                               tttGAACTCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGttt                                                                                                                    <  2:12654/149‑1 (MQ=255)
                                 tGAAATCGGTGTCTTCTTAGCCCGCTTTTACGTTTCTTAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAATAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGa                                                                                                                  <  2:654757/149‑1 (MQ=255)
                                 tGAAAGCGCTGTCTGCTTCTCACGCGTTTACGTTGGTACAGGGCGAAATCGGGAAGAGGAAAACTACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGa                                                                                                                  <  2:657645/149‑1 (MQ=255)
                                   aaaTCGGTGTCTTCTTCTCACGCTTTTACGTTGTTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGata                                                                                                                >  2:610783/1‑149 (MQ=255)
                                     aTCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATc                                                                                                              >  1:1006454/1‑149 (MQ=255)
                                        ggTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGc                                                                                                           <  1:266323/149‑1 (MQ=255)
                                              ttcttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAt                                                                                                     >  1:942116/1‑149 (MQ=255)
                                              ttcttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAt                                                                                                     >  2:278810/1‑149 (MQ=255)
                                                cttctCACGGTTTTACGTTGGTAAAGGGCGAAATGGGGAAGAGGAAAACGACGCTGAAGCAGAAATCGAAAAAAGTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAttt                                                                                                   <  1:908970/149‑1 (MQ=255)
                                                cttctCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAttt                                                                                                   >  1:841171/1‑149 (MQ=255)
                                                   ctcACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAc                                                                                                >  2:878860/1‑149 (MQ=255)
                                                     ccagCTTATACGTTGGTATAGGCCGAAATCGGGAAGAGGATAACGAGGCTGAAGCATAAAGCGAAAAAACTGAAGAATAAATTCAACCGGCCGTGAGGGCGGGAGGCATATGGAGAACAGGATGTTTGATTTCGTCGGTAGTTTgtcac                                                                                              >  2:208518/4‑145 (MQ=255)
                                                         cgtttACGTTCGGAAAGGGCGCAATCGGGAAGCTGTAAAAGACGCTGAAGCAGAAATCGCAAAACCTGAAGACTAAATTCAACCGCCCGTCAGGGGGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCCGtgc                                                                                          <  1:774818/147‑3 (MQ=255)
                                                              aCGTGGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCCGTTCGCAAt                                                                                     >  1:819632/1‑149 (MQ=255)
                                                               cGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAGCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGGACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAAtt                                                                                    >  1:786706/1‑149 (MQ=255)
                                                                      aaaGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGATGTCATATGGAGTAAAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaaa                                                                             >  2:691158/1‑149 (MQ=255)
                                                                      aaaGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAAAAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGaaa                                                                             <  2:738481/149‑1 (MQ=255)
                                                                        aGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAAATTGCGaactg                                                                           <  2:386418/149‑4 (MQ=255)
                                                                            cGAAATCGGGAAGAGGAAAACGCCGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGTAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGt                                                                       >  1:829873/1‑149 (MQ=255)
                                                                                    ggAAGAGGAAAACGACTCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGTGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgt                                                               <  2:946920/149‑1 (MQ=255)
                                                                                    ggAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgt                                                               <  2:1042087/149‑1 (MQ=255)
                                                                                    ggAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgt                                                               >  2:796480/1‑149 (MQ=255)
                                                                                    ggAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATACAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGAGGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTGGCGAAAGACCGTGAGGAtgt                                                               <  2:85067/149‑1 (MQ=255)
                                                                                     gAAGAGGAAATCGACGCTGACTCAGATATCGAAAAAACTTAAGAATACATTCACCCGGCCGGCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgtt                                                              <  2:653371/149‑1 (MQ=255)
                                                                                     gAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAATACAGTGATGAtgtt                                                              >  1:104588/1‑149 (MQ=255)
                                                                                     gAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGCTGAggtt                                                              >  1:108742/1‑149 (MQ=255)
                                                                                     gAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgtt                                                              <  1:578815/149‑1 (MQ=255)
                                                                                     gAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgtt                                                              >  1:126171/1‑149 (MQ=255)
                                                                                       agagGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGAtgttgt                                                            <  2:1167465/149‑1 (MQ=255)
                                                                                          ggAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGc                                                         >  1:182057/1‑149 (MQ=255)
                                                                                           gAAAACGCCGCTGCAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGCTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGAGGATGTTGTAGCg                                                        >  2:856754/1‑149 (MQ=255)
                                                                                           gAAAACGACGCTGAAGCAGAACGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGAGGTTGTCATATGGAGTACAGGATGTTTGCTATCGTCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCg                                                        >  1:704009/1‑149 (MQ=255)
                                                                                           gAAAACGACGCTGAAGCAGAAAGCGAAACACCTGAAGAATACATTCAACCGCCCGTCCGGGCGGTTGTCATCTGGAGTACAGGATGTGTGAGAGCGGGGGTAATTTGCCCAGTGCGCCATTTGCGAAAGTCCTTGATGATGTTGTAGCg                                                        >  2:650232/1‑149 (MQ=255)
                                                                                           gAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACATGATGTTTGATATCGGCTTTAATTTGCCCAGTTCTCAATTTGCGAAAGACCGTGATGATGTTGTAGCg                                                        >  2:504385/1‑149 (MQ=255)
                                                                                            atcacgccgCTGAAGCAGACAGCGAAACAACTGCAGAATAACTTCAACCCCCCGTCTCGGCGGTTGGCATATGTAGTACAGGCTGTTTGATATGGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGt                                                       <  2:114241/146‑1 (MQ=255)
                                                                                               acgacgCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGCTGTAGCGTgcg                                                    <  1:567047/149‑1 (MQ=255)
                                                                                                  acgCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGAGGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCtt                                                 >  2:957783/1‑149 (MQ=255)
                                                                                                    gCTGAAGCAGAAAGCGAAAAAAGTGAAGAATAAATTCAACCGCCCGTCTGGGGGGTTGTCTTATGGAGTAGAGGTTGTTTGATATCGGCGTTTATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCtgtt                                               >  2:352737/1‑149 (MQ=255)
                                                                                                     cTGAAGCAGAATGCGAAAACACTGAATAATAAATTCAACCGCCCGTCATGGCGGTTGTCATATGGAGTACAGGATCTTTGAAATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGGAGCGTGCGCTTTTg                                              <  2:893822/149‑1 (MQ=255)
                                                                                                      tGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGa                                             >  1:448190/1‑149 (MQ=255)
                                                                                                      tGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTAGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTATCGTGCGCTTTTGa                                             >  2:310207/1‑149 (MQ=255)
                                                                                                          tcAGATAGCGAAATAACTGAAGATTTAATTCTACCGCGCGTCAGGGCGGTTGTCATATGGAGTACTGGATGTTTGTTATGGGCGTTAATTTGACCAGTTCGCAAAATGCGAAAGACCGTGATGCTCTTGTAGCGTGCGCTTTTGAcgcg                                         <  1:798850/148‑1 (MQ=255)
                                                                                                          gCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCAATTGGAGTACAGGATGTTTGATATCTGCGTTAATTTGACCAGTTCACAATTTGAGCAAGACCCTGAGGATGGTCTAGCGTGCGCTGTAGCcgcg                                         >  2:1104790/1‑149 (MQ=255)
                                                                                                          gCACAAAGCGAAAAAACTGAAGAATAACTTCACCCGCCCGGCAGGGCGGTTGTCATATGGAGTACACGATGTTTGATCTCGGCGTTAATTAGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTGGTAGCGTGCGCTTTTGAcgcg                                         <  2:1090231/149‑1 (MQ=255)
                                                                                                           cAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCgg                                        <  1:458905/149‑1 (MQ=255)
                                                                                                           cAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCgg                                        <  1:605712/149‑1 (MQ=255)
                                                                                                           cAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGGGATGATGTTGTAGCGTGCGCTTTTGACGCgg                                        <  1:610783/149‑1 (MQ=255)
                                                                                                           cAGAAACCGACACAACTGAAGACTAACTTCAACCGCCCGTCAGGGCGGATCTCAAATGGCGTACAGGATGTTTGCTATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCgg                                        <  1:1187064/149‑1 (MQ=255)
                                                                                                              tatgCGAAAAAACTGAAGAATAAAATCAACCGCCCTTCAGGGCGGTTGTCATATGTAGTACAGGATGTTTGATATCGGCGTTAATTTTACCAGTTCGCAGTTTGCGAAAGAGCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAg                                     <  2:610513/146‑1 (MQ=255)
                                                                                                              aaaGCGAAAAAACTGAAGAATTAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAg                                     <  2:108171/149‑1 (MQ=255)
                                                                                                              aaaGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGATCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCTTGCGCTTTTGACGCGGGAg                                     >  1:1178213/1‑149 (MQ=255)
                                                                                                               aaGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGATTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGAAATTTGCGAAAGACAGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGt                                    <  2:645951/149‑1 (MQ=255)
                                                                                                                    aaaaaaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATg                               >  2:232494/1‑149 (MQ=255)
                                                                                                                    aaaaaaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATg                               <  1:276405/149‑1 (MQ=255)
                                                                                                                    aaaaaaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATg                               >  2:355064/1‑149 (MQ=255)
                                                                                                                    aaaaaaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGAGGTTTGATTTCGGCGTTAATTTGACCAGTTCGCAATATGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATg                               <  1:26843/149‑1 (MQ=255)
                                                                                                                       aaaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCCTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCATTTGACGCGGGAGTTAATggtc                            >  1:868201/1‑147 (MQ=255)
                                                                                                                        accTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCt                           <  2:903124/147‑1 (MQ=255)
                                                                                                                        aaCTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATgggat                           >  2:138774/1‑147 (MQ=255)
                                                                                                                           tGAAGAATAATTTGAACGGCCGGTCAGGGCGGTTGTCATATGGAGTTCAGGATGTTTGTTATCGGCGTTAATTTGACCAGTTCGGAATTTGCGAAAGTCCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACt                        <  1:939750/149‑1 (MQ=255)
                                                                                                                           tGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACt                        >  2:753494/1‑149 (MQ=255)
                                                                                                                           tGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACt                        >  1:575232/1‑149 (MQ=255)
                                                                                                                           tGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACAAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACt                        >  1:948163/1‑149 (MQ=255)
                                                                                                                                 aTAAATTCAACCGCCCGCCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCAc                  <  1:689631/149‑1 (MQ=255)
                                                                                                                                    aaTTCACCCGCCCGGCAGGGCGGTTGTCAAATGGAGTAGAGGAGGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCgg               <  2:82112/149‑1 (MQ=255)
                                                                                                                                     aTTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGc              <  2:291287/149‑1 (MQ=255)
                                                                                                                                      ttCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCa             >  2:74650/1‑149 (MQ=255)
                                                                                                                                      ttCAACCGCCAGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGAGTTAATTTGACCAGTTCGCAAATTGCGAAAGACAGTGATGATGTTGTAGCGAGCCCATTTGACGAGGGAGTTAATGGGCTACTCATCACCGGCa             >  2:680622/1‑149 (MQ=255)
                                                                                                                                       acAACCGCCCTGACGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCTTGATGATGTTGTAGCGTGCGCTTTTGACGAGGGAGTTAATGGGCTAATAATCACCGGCAc            >  2:608041/2‑149 (MQ=255)
                                                                                                                                        cAACCGCCCTGACGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACt           >  1:554224/1‑149 (MQ=255)
                                                                                                                                          aCCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGTTGTTTGATATCCGCGTTAATTTGACAAGTTCGCAAATTGCGAAAGACCGTGATCATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACtta         >  1:70052/1‑147 (MQ=255)
                                                                                                                                               ccGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCGCCGGCACTAACCTGc    >  2:602548/1‑149 (MQ=255)
                                                                                                                                                cGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCg   <  2:908656/149‑1 (MQ=255)
                                                                                                                                                cGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTCGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCg   <  2:942116/149‑1 (MQ=255)
                                                                                                                                                 gTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCGt  >  1:300425/1‑149 (MQ=255)
                                                                                                                                                 gTCAGGGCGGTTGTCATATGGAGTACACGATGTTTGATATCGGCGTTAATTTGACCTGTTCGCAATTTGCGTAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCGt  <  2:745964/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGACCAGTTCGCAATTTGCGAAAGACCGTGATGATGTTGTAGCGTGCGCTTTTGACGCGGGAGTTAATGGGCTACTCATCACCGGCACTAACCTGCGT  >  NC_000913/4023380‑4023673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: