Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,291,513 T→C 6.4% M1A (GTG→GCG) † nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,291,5130TC 6.4% 215.6 / 4.0 77M1A (GTG→GCG) †nrfEheme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
Reads supporting (aligned to +/- strand):  ref base T (40/32);  new base C (2/3);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

CCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTC  >  NC_000913/4291365‑4291660
                                                                                                                                                    |                                                                                                                                                   
ccAGTCTGGTCGGCGTGTTGAGGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACgt                                                                                                                                                     <  1:790929/149‑1 (MQ=255)
    tCTGGTCGGCGTGTTGATGCTGCGCTTTTACATTCTCTACGCCGTACAGTTGACGGGGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTg                                                                                                                                                 <  2:107327/149‑1 (MQ=255)
       ggTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGGGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGAcc                                                                                                                                              <  1:62218/149‑1 (MQ=255)
        gTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGGGTAAGCCAGAAATGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTGGGGGGCAACACGTTGAccc                                                                                                                                             <  2:1169583/149‑1 (MQ=255)
        gTCGGCGCGCTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACACGTTGAccc                                                                                                                                             <  1:150607/149‑1 (MQ=255)
         tCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGAcccc                                                                                                                                            >  2:498767/1‑149 (MQ=255)
          cGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCGGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCg                                                                                                                                           <  1:1064259/149‑1 (MQ=255)
            gCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGtt                                                                                                                                         >  2:840760/1‑149 (MQ=255)
            gCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGtt                                                                                                                                         <  1:514607/149‑1 (MQ=255)
               tgtTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGAc                                                                                                                                      <  1:1105591/149‑1 (MQ=255)
                gtTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACg                                                                                                                                     >  2:305467/1‑149 (MQ=255)
                 ttGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACgg                                                                                                                                    >  2:448264/1‑149 (MQ=255)
                 ttGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACgg                                                                                                                                    >  1:382477/1‑149 (MQ=255)
                  tGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTGCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGc                                                                                                                                   <  2:1031019/149‑1 (MQ=255)
                     tgctgcGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCtt                                                                                                                                >  2:279414/1‑149 (MQ=255)
                       ctgcGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTcg                                                                                                                              >  1:139914/1‑149 (MQ=255)
                       ctgcGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTcg                                                                                                                              >  1:422125/1‑149 (MQ=255)
                        tgcGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGAGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCCTTGTTGTTAAGTCTCGGGGTCAACGTGTTGCCCCCGTTGACGGCCTTcgc                                                                                                                             >  1:64779/1‑149 (MQ=255)
                         gcgcTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTcgcg                                                                                                                            >  2:621401/1‑149 (MQ=255)
                          cgcTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCgg                                                                                                                           <  1:970713/149‑1 (MQ=255)
                          cgcTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCgg                                                                                                                           <  1:958653/149‑1 (MQ=255)
                            ctgtttctttcTCTACGCCGGACAGTTGACGGTGGCGTAAGCGAGAAACGAGGTGGTTTCTGGACGTATTCCTTCCTGACGTCGGTTTGCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGa                                                                                                                         <  1:777553/146‑1 (MQ=255)
                            ccgatataTTCTCTACGCCGGCCAGTTGTCGGTGGCGTAAGCCAGTAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGa                                                                                                                         <  2:931888/142‑1 (MQ=255)
                             tttttCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTTTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAg                                                                                                                        >  1:990900/1‑149 (MQ=255)
                             tttttCATTCGCTACGCCGTACAGTTGACGGGGGCGTAAGCCAGAAAAGTGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAg                                                                                                                        <  1:526375/149‑1 (MQ=255)
                             tttttAATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGCACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGTTCAAAGTCTTGACCCCGTTCACGGCCTTCGCGGGAg                                                                                                                        >  1:876015/1‑149 (MQ=255)
                              ttttCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGt                                                                                                                       >  1:1107930/1‑149 (MQ=255)
                               tttCAATCTCTTCGCCGGACAGTTGACGGGGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGCCGTCGGTGTGCTGGCGTTGTTGTGACGTCGCGGGGGCAACGCGTTGACCCCGTTGACGGCCTTCGCGGGAGTg                                                                                                                      <  1:38956/149‑1 (MQ=255)
                                   aTTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGTGAGTGCGGt                                                                                                                  >  2:640825/1‑149 (MQ=255)
                                    ttCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAgagatcgg                                                                                                                 >  1:931888/1‑142 (MQ=255)
                                    ttCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGtt                                                                                                                 <  2:986599/149‑1 (MQ=255)
                                           cGCCGGACAGTTGACGGTGTCGGAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGGTGTTGTTAAGTCTCGGGGGCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTgg                                                                                                          <  1:915703/149‑1 (MQ=255)
                                              cGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGcat                                                                                                       >  1:612095/1‑147 (MQ=255)
                                              cGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCt                                                                                                       >  1:702150/1‑149 (MQ=255)
                                                gACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCATTCCTGAAGTCAGTTTTCTGGCGTTGTTGTTAAGTCTCTGGGTCAACTTGTTGACACCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGATGGCCTGc                                                                                                     >  2:649266/1‑149 (MQ=255)
                                                  cAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTGTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGGTGTTGTTAAGTCTCGCGGTCAACGTGTTGACCCGGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCa                                                                                                   <  2:483658/149‑1 (MQ=255)
                                                   aGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCat                                                                                                  <  2:422125/149‑1 (MQ=255)
                                                       gACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCatgatg                                                                                              >  1:280111/1‑149 (MQ=255)
                                                       gACGGTGGCGGAAGCCAGCAAAGAGGTGGTTTCGGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCatgatg                                                                                              <  1:900195/149‑1 (MQ=255)
                                                        aCGGTGGCGTAAGCCAGAAACGAGGGGGGTTCTGGACGTATTCCTTCCGGAAGTCGGGTTTCTGGCGTTGTTGTTACGTCTCGGGGGCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGc                                                                                             <  1:642946/149‑1 (MQ=255)
                                                          ggTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGa                                                                                           >  1:924144/1‑149 (MQ=255)
                                                           gTGGCGTAAGCCAGAAAAGAGTTGGTTTCTGGACGAATTCATTCCTGTAGTCGGTGTTCTGGCGTTGTTGTTAAGTCTCGGGGGCAACGTGTGGACCCCGTTGACGGACTGCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGAc                                                                                          <  1:1082287/149‑1 (MQ=255)
                                                                gTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACt                                                                                     >  1:1138322/1‑149 (MQ=255)
                                                                     ccAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCAt                                                                                >  1:323360/1‑149 (MQ=255)
                                                                                  ggtTTCTGGACGTATTCCTTCCTGAAGGCGGTTTTCTGGCGTTGTTGTTAAGTGTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGgcg                                                                   <  2:439340/149‑1 (MQ=255)
                                                                                     ttCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCCTTCTGGCGCAg                                                                >  1:755847/1‑149 (MQ=255)
                                                                                        tGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTGAGCGGGAGTGCGGTTGCGCTGGCCTGCACTGATGCGAAGAACTTGAAGAGTGATTCTGGCGCAGTTc                                                             >  2:864356/1‑149 (MQ=255)
                                                                                                ttccttccTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAAAGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCTGCATTCTTGCTCAGTTCGCGCTCct                                                     >  1:401854/1‑149 (MQ=255)
                                                                                                  ccttcctGAAGTCGGTTTTCTGGCGTTGTTGTTACGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCctgc                                                   <  1:879262/149‑1 (MQ=255)
                                                                                                      cctGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGACATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTgctgct                                               >  1:555947/1‑149 (MQ=255)
                                                                                                       ctGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCCTTCTGGCGCAGTTCGCGCTCCTGCTGCTc                                              >  2:1054337/1‑149 (MQ=255)
                                                                                                             tCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCttt                                        >  1:1031217/1‑149 (MQ=255)
                                                                                                                      tGGCGTTGTAGTTAAGTCTCGGGGTCAACGTGTTGAACCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCTCTTGCATCGGCTTTCTGGCGGAGTTCGCGCTCCTTCTGCTAGCCTTAGGCGTACTg                               >  1:1112254/1‑149 (MQ=255)
                                                                                                                        gCGTTGTTGTTAAGTCTCGGGGTCAACGTGTGGACCCCGTTGACGGCCTTCGCGGGAGGGCGGGTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGAc                             <  1:135549/149‑1 (MQ=255)
                                                                                                                          gttgttgttAAGTCTCGGGGTCAACGTGTTGCCCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGt                           >  1:942429/1‑149 (MQ=255)
                                                                                                                             gttgttAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTAtt                        <  2:702150/149‑1 (MQ=255)
                                                                                                                             gttgttAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTAtt                        >  1:31408/1‑149 (MQ=255)
                                                                                                                                gttAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCTTTCTTGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGtt                     <  1:84952/149‑1 (MQ=255)
                                                                                                                                 ttAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCAGTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTGTGGCGTACTGACGTATTGttt                    >  2:495425/1‑149 (MQ=255)
                                                                                                                                  tAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGGGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGtttt                   <  1:640825/149‑1 (MQ=255)
                                                                                                                                    aGTCTCGGGGTCCACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGAGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATAGGAAATCTGTCGCAGTTCGCGCTCCGGCTGCTCGCCTGTGGCGAACTTACGTATAGTTTtct                 >  1:727989/1‑149 (MQ=255)
                                                                                                                                    aGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTtct                 <  2:1107930/149‑1 (MQ=255)
                                                                                                                                     gTCTCGGGGTAAACGTGTTGACCCCGGTGTCGGCCTACGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTtctc                <  1:670601/149‑1 (MQ=255)
                                                                                                                                      tctcGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCtca               >  2:253995/1‑149 (MQ=255)
                                                                                                                                      tctcGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCtca               >  2:352282/1‑149 (MQ=255)
                                                                                                                                      caacGGGGTCAACACGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCtca               >  2:318153/4‑149 (MQ=255)
                                                                                                                                        acGGGGTCAACACGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTCGCGTACTGACGTATTGTTTTCtcatc             >  1:59981/2‑149 (MQ=255)
                                                                                                                                           gggTCAACGTGTTGACCGCGTTGACGGCCTTCGAGGGAGGGCGGTTGCGCTGGCCTGACATGATGCGACTCTCTTGCATCGTCATTATGGCGCATTTCGCGCTCCTGCTGCTCGCCTATGGCGTACTGACGTATTGTTTTCTCATCAGc          <  2:255650/149‑1 (MQ=255)
                                                                                                                                           gggTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGc          <  1:649266/149‑1 (MQ=255)
                                                                                                                                            ggTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCg         <  2:382477/149‑1 (MQ=255)
                                                                                                                                             gTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTtct                 <  1:1158237/140‑1 (MQ=255)
                                                                                                                                             gTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTtct                 >  2:1158237/1‑140 (MQ=255)
                                                                                                                                             gTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGa        >  2:200040/1‑149 (MQ=255)
                                                                                                                                              tCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCTGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGAt       <  2:611296/149‑1 (MQ=255)
                                                                                                                                                aaCGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCGCTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGCttt     >  1:949976/1‑149 (MQ=255)
                                                                                                                                                aaCGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGAttt     >  1:850657/1‑149 (MQ=255)
                                                                                                                                                aaCGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACGGACGTATTGTTTTCTCATCCGCGAgtt     >  1:1174891/1‑149 (MQ=255)
                                                                                                                                                 aCGTGTGGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTtc    <  1:279414/149‑1 (MQ=255)
                                                                                                                                                   gtgtTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGAGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTtctc  >  1:732622/1‑149 (MQ=255)
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CCAGTCTGGTCGGCGTGTTGATGCTGCGCTTTTTCATTCTCTACGCCGGACAGTTGACGGTGGCGTAAGCCAGAAAAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGTTGTTAAGTCTCGGGGTCAACGTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCTGGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTC  >  NC_000913/4291365‑4291660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: