Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,366,854 T→G 7.9% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,8540TG 7.9% 249.9 / 9.2 97intergenic (‑81/+37)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (48/40);  new base G (2/6);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

CCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCCAG  >  NC_000913/4366706‑4367001
                                                                                                                                                    |                                                                                                                                                   
ccTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAt                                                                                                                                                     <  2:157247/149‑1 (MQ=255)
 cTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAACGGCACGTCATc                                                                                                                                                    <  1:902602/149‑1 (MQ=255)
        ccccAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTGTTTAACAAGTTGATATTAGATTGTTTTTTTTAAGTTACTGCTCACAAGAAAAAAGGCCCGTCATCTGACGTg                                                                                                                                             >  1:794605/1‑149 (MQ=255)
        ccccAATCTGGCGCCCAAGAAGATCACCAGCAAAACTATGATGAGTTCTACAACTAGCATATAAGCCTACCTTGTTTTTTAACAAGTTGATATTAGATTTTAATATTTAAGTTACTGCTAACAAGAAAAAAGGCACGTAAACTGACCTg                                                                                                                                             >  1:417469/1‑149 (MQ=255)
           cAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATTATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCt                                                                                                                                          <  1:683815/149‑1 (MQ=255)
            aaTCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCtt                                                                                                                                         <  1:610501/149‑1 (MQ=255)
              tCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCtttt                                                                                                                                       <  2:367134/149‑1 (MQ=255)
               cTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGACTTCTACAACTAGCATATTCGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                                                                                                                      <  1:565276/149‑1 (MQ=255)
                 ggCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTa                                                                                                                                    >  2:261092/1‑149 (MQ=255)
                 ggCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTa                                                                                                                                    >  2:344773/1‑149 (MQ=255)
                 ggCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCttttttc                                                                                                                                    <  2:247686/149‑2 (MQ=255)
                  gcgcCCAAGAAGATCGCCAGCAAATCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTATTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGGCATCTGACGTGCCTTTTTTAt                                                                                                                                   <  2:683508/149‑1 (MQ=255)
                  gcgcCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCttttttct                                                                                                                                   <  2:995874/149‑3 (MQ=255)
                  gcgcCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCAGATGACGTGCCttttttct                                                                                                                                   <  1:397593/149‑3 (MQ=255)
                     cccAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTg                                                                                                                                <  2:1018400/149‑1 (MQ=255)
                       cAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGta                                                                                                                              >  1:333510/1‑149 (MQ=255)
                         agaagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtact                                                                                                                            >  1:1024658/1‑149 (MQ=255)
                           aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtactac                                                                                                                          <  1:192856/149‑1 (MQ=255)
                           aagaTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGtactac                                                                                                                          >  2:1184646/1‑149 (MQ=255)
                            ataTCGCCAGCAAAACTATTATGAGTTATACAACTAGCATATTAGCCTTCCATGTTTTTTAACAAGTTGATAATAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAcc                                                                                                                         <  1:398639/147‑1 (MQ=255)
                              aTCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTTCTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCt                                                                                                                       >  1:76063/1‑149 (MQ=255)
                               tCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCAAGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTg                                                                                                                      >  1:717911/1‑149 (MQ=255)
                                 gCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGCCGTGCCTTTTTTATTTGTACTACCCTGTa                                                                                                                    >  2:791660/1‑149 (MQ=255)
                                  ccAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGTCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTAc                                                                                                                   >  1:537330/1‑149 (MQ=255)
                                  ccAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTAc                                                                                                                   >  1:322498/1‑149 (MQ=255)
                                     gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGAt                                                                                                                >  1:640132/1‑149 (MQ=255)
                                     gCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATAGTTATTTTTAAGTTACTGCTAACAAGAAAAAAGGCACGTCATCAGACGTGCCTTTTATATATGTACTACCCTGTACGAt                                                                                                                >  2:374885/1‑149 (MQ=255)
                                         aaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACATGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACt                                                                                                            >  2:94480/1‑149 (MQ=255)
                                         aaCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACt                                                                                                            >  2:474125/1‑149 (MQ=255)
                                          aCTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTg                                                                                                           >  2:683034/1‑149 (MQ=255)
                                           cTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGt                                                                                                          >  1:985795/1‑149 (MQ=255)
                                            tATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCATGTACGATTACTGtt                                                                                                         >  1:1852/1‑149 (MQ=255)
                                                atgaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATATGACGTGCCTTTTTTATTTGTACTACCCTGTACGAGTACTGGTCGCt                                                                                                     >  2:818456/1‑149 (MQ=255)
                                                atgaGTTCTAAAACTAGCATATTAGCCTTCCTTGTTGTTATACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCt                                                                                                     <  2:698076/149‑1 (MQ=255)
                                                  gaGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCttt                                                                                                   <  1:1092733/149‑1 (MQ=255)
                                                   aGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtc                                                                                                  <  2:1024658/149‑1 (MQ=255)
                                                       cTACAACTAGCATATTAGCCTTGCTAGTTTTTTAACAAGTTGATTTTAGATTGTTCTTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatca                                                                                              <  2:987574/149‑1 (MQ=255)
                                                       cTACAACTAGAATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatca                                                                                              <  1:5109/149‑1 (MQ=255)
                                                        tACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAg                                                                                             >  1:31106/1‑149 (MQ=255)
                                                          cAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGta                                                                                           <  2:322498/149‑1 (MQ=255)
                                                          cAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGta                                                                                           <  2:275144/149‑1 (MQ=255)
                                                            aCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTCGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGtata                                                                                         >  1:584960/1‑149 (MQ=255)
                                                              tAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGc                                                                                       >  1:185435/1‑149 (MQ=255)
                                                              tAGCATATTAGCCTTCATTGTTTTTTAACAAGTTGATATTAGATAGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGAAGTGACTTTTTTTTTTGTACTAGCCTTTACTATTAATTTTCTATTTAATCAGtatatc                                                                                       >  1:713499/1‑147 (MQ=255)
                                                                 cATATTATCCTTCCTTGTTTTTTAACTAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGtt                                                                                    <  2:270651/149‑1 (MQ=255)
                                                                  atatTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGttt                                                                                   <  2:1093800/149‑1 (MQ=255)
                                                                   tatTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGtttt                                                                                  >  2:562314/1‑149 (MQ=255)
                                                                      tAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGAATGTTATATTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCt                                                                               <  1:269313/149‑1 (MQ=255)
                                                                       aGCCTTCCTTGTTTTTTATCAAGTTGATATTAGATTGTTATTTTTAAGTTACTTCTCACAAGAAAACAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTTCCCTGTTCGTTTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtt                                                                              <  2:417469/149‑1 (MQ=255)
                                                                       aGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtt                                                                              <  1:844532/149‑1 (MQ=255)
                                                                        ggcttccttGTTTTTTTACAAGTTGATTTTTGATTTTTATTTTTTAGTCACTGCTCCAAAGTAAATATGCAGGTCTTCTGACGTGGCTTTTTTATTTGTACTACCCTGTACGATTACTGTTTGCTTTCATCAGTATAGCGTTTTGCttt                                                                             <  1:674479/147‑1 (MQ=255)
                                                                        gCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGGCAGCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCttt                                                                             <  2:210786/149‑1 (MQ=255)
                                                                         ccttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTTCTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTg                                                                            >  1:976466/1‑149 (MQ=255)
                                                                         ccttccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTAATCTGACGTGCCGTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCtttt                                                                            >  1:559613/1‑148 (MQ=255)
                                                                            tccttGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTaa                                                                         <  2:632160/149‑1 (MQ=255)
                                                                               ttGTTTTATAAAAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGcc                                                                      <  2:698295/149‑1 (MQ=255)
                                                                                tGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTCAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCg                                                                     <  1:1004476/149‑1 (MQ=255)
                                                                                 tttttttAACAAGTGGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGAACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCgg                                                                    <  2:558573/148‑1 (MQ=255)
                                                                                  ttttttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCggg                                                                   >  1:358317/1‑149 (MQ=255)
                                                                                    ttttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTg                                                                 <  1:1013597/149‑1 (MQ=255)
                                                                                    ttttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTg                                                                 >  2:433930/1‑149 (MQ=255)
                                                                                     tttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGc                                                                <  1:1015325/149‑1 (MQ=255)
                                                                                     tttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGc                                                                >  2:945472/1‑149 (MQ=255)
                                                                                      ttAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCa                                                               >  1:232080/1‑149 (MQ=255)
                                                                                       tAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCTTTTTGCTTTGTAAGCCGGGTGCAt                                                              >  2:784602/1‑149 (MQ=255)
                                                                                           aaGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGa                                                          >  1:160562/1‑149 (MQ=255)
                                                                                              ttGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTc                                                       <  1:1057871/149‑1 (MQ=255)
                                                                                              tagATATTAGATTGATATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCGTATTTATTTGTACTACCCTGTACTATTAATGTTCGATTTCATCAGTATAGCGTTATGCTTTGTAAGCCGGGTGCATCAGaata                                                       >  2:994087/3‑146 (MQ=255)
                                                                                               tGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTGATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCt                                                      >  2:110480/1‑149 (MQ=255)
                                                                                                 ttatTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt                                                    <  1:148650/148‑1 (MQ=255)
                                                                                                 atatTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGt                                                    <  2:640132/149‑1 (MQ=255)
                                                                                                   atTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACTTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGTATCAGATTCTGTAc                                                  <  1:395941/149‑1 (MQ=255)
                                                                                                        aTTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGAGGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATATCGTTTTGCTTTGTAAGCCGGGTGCAGCAGATTCTGTACGgaga                                             >  1:1058220/1‑149 (MQ=255)
                                                                                                            ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAat                                         >  2:152864/1‑149 (MQ=255)
                                                                                                            ttATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTACGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAat                                         >  1:134781/1‑149 (MQ=255)
                                                                                                                ttttAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCg                                     <  1:883034/149‑1 (MQ=255)
                                                                                                                  ttAATTTTCTGCTCACAATAAAAAAGGCACGTCATCTGACGTTCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTc                                   <  1:1164961/149‑1 (MQ=255)
                                                                                                                    aaGTTACTGCTCACAACAAAAAATGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATCTCGTCaa                                 <  2:376578/149‑1 (MQ=255)
                                                                                                                       ttACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGtt                              >  1:316408/1‑149 (MQ=255)
                                                                                                                         aCTGCTCACAAGAAAAACGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTATCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTcc                            <  2:1125354/149‑1 (MQ=255)
                                                                                                                           tGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGc                          >  1:622017/1‑149 (MQ=255)
                                                                                                                             cTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCtt                        <  1:791660/149‑1 (MQ=255)
                                                                                                                               acaaataaaaaaGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTTCGGAGAAAATATGGTCAAGTTCCGCTTCa                      >  1:499734/7‑149 (MQ=39)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCttt                                                                                                   >  2:419004/1‑71 (MQ=255)
                                                                                                                                acaAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCttt                                                                                                   <  1:419004/71‑1 (MQ=255)
                                                                                                                                 caAGAAAAATGGGACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTTAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGt                    <  2:794605/149‑1 (MQ=255)
                                                                                                                                  aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc                   >  2:528633/1‑149 (MQ=255)
                                                                                                                                  aaGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTc                   >  1:649665/1‑149 (MQ=255)
                                                                                                                                     aaaaaaGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCAGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAAc                >  2:689696/1‑149 (MQ=255)
                                                                                                                                          aGGCACGTCAGATGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTAGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGAATCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCATTCAACAGAcc           >  2:673687/1‑149 (MQ=255)
                                                                                                                                            gCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTACATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACcgc         >  1:456589/1‑149 (MQ=255)
                                                                                                                                              aCGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCATATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGt       >  1:812553/1‑149 (MQ=255)
                                                                                                                                                gTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTc     <  2:31106/149‑1 (MQ=255)
                                                                                                                                                gTCATCTGACGTGCCTTTTTTATTTGTACTACACTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTc     >  1:557769/1‑149 (MQ=255)
                                                                                                                                                 tCATCTGCCGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTcc    >  1:141801/1‑149 (MQ=255)
                                                                                                                                                  cATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCCa   <  2:76063/149‑1 (MQ=255)
                                                                                                                                                   aTCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCCAg  <  1:562314/149‑1 (MQ=255)
                                                                                                                                                   aTCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGATTTCATCAGTATAGCGTTTTGCTTTGTACGCCGGGTGCATCAGATTCTGTACGGAGTAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCCAg  >  1:554452/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTATAGCGTTTTGCTTTGTAAGCCGGGTGCATCAGATTCTGTACGGAGAAAATATCGTCAAGTTCCGCTTCAGTCAACAGACCGCGTTCCAG  >  NC_000913/4366706‑4367001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: