Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,400,138 A→C 7.4% D296A (GAC→GCC)  miaA → delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,400,1380AC 7.4% 212.6 / 5.6 85D296A (GAC→GCC) miaAdelta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
Reads supporting (aligned to +/- strand):  ref base A (41/35);  new base C (2/5);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAG  >  NC_000913/4399990‑4400286
                                                                                                                                                    |                                                                                                                                                    
cGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGa                                                                                                                                                      >  2:617082/1‑149 (MQ=255)
cGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGa                                                                                                                                                      <  1:1174707/149‑1 (MQ=255)
 gTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGAc                                                                                                                                                     >  2:1097947/1‑149 (MQ=255)
     cGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTg                                                                                                                                                 >  1:872533/1‑149 (MQ=255)
      gTGGGGTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAAGGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGGTCACTGGCTTGACAGTGa                                                                                                                                                <  1:874244/149‑1 (MQ=255)
       tGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGTCAAGCCAGTGaa                                                                                                                                               <  1:1016879/149‑1 (MQ=255)
       tGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGTCAAGCCAGTGaa                                                                                                                                               <  1:552421/149‑1 (MQ=255)
       ggggTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGGTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGTCAAGCCAGTGaa                                                                                                                                               <  1:201930/148‑1 (MQ=255)
        gggTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGATTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGaaa                                                                                                                                              <  2:3445/149‑1 (MQ=255)
        gggTAATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGAGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGTCAAGCCAGTGaac                                                                                                                                              <  2:726074/149‑2 (MQ=255)
          ggtctCGCCAGATGTGGTCTTACCTTGACCGCGAAATCTCATACGATGAAATGGTTTATCGAGGGGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAACGGGTGCACTGGCTTGACAGTGaaaaa                                                                                                                                            <  1:735434/145‑1 (MQ=255)
             aTCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGCTGAAATGGTATATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCa                                                                                                                                         <  1:835879/149‑1 (MQ=255)
                   aGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAg                                                                                                                                   >  1:896468/1‑149 (MQ=255)
                   aGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAg                                                                                                                                   >  1:67933/1‑149 (MQ=255)
                   aGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGGTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATACCCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAg                                                                                                                                   <  1:693261/149‑1 (MQ=255)
                     aTGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGgc                                                                                                                                 >  1:672443/1‑149 (MQ=255)
                            cTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGAc                                                                                                                          >  1:419852/1‑149 (MQ=255)
                             ttACCTTGAAGGCGAAATCTCATAAGATGAAATGGTTTATCTAGCTGTTTGCGCCACGAGACAGTTGGCGAAGAGGCAGATAACCAGGCTGCTTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACAAGAACAGGCGCGTGACg                                                                                                                         >  2:493564/1‑149 (MQ=255)
                              tACCTTGAAGGCGAAAGCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGa                                                                                                                        <  2:453026/149‑1 (MQ=255)
                               aCCTTGAAGGCGAATTCTCATACGATGAAATGGTTTATCGAGGTGGTTGCGCCACGAGACAGTTGGCTAAGCGGCAGATAACCTGGCTGCGTGGTTGGTAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGaa                                                                                                                       <  2:591350/149‑1 (MQ=255)
                                ccatgcaGGCGAAATATCATACGAGGAAATGGCCTCTCGCGGGGTTTGCGCCACGAGACCGTTGGCGAAGAGGCAGATAACCTGGCTGCGTTGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGATCAGGCGCGTGACGAAg                                                                                                                      <  2:981938/143‑1 (MQ=255)
                                ccTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAg                                                                                                                      <  2:1056581/149‑1 (MQ=255)
                                ccTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAg                                                                                                                      <  1:196929/149‑1 (MQ=255)
                                ccTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGGGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAg                                                                                                                      <  1:133189/149‑1 (MQ=255)
                                 cTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGt                                                                                                                     >  1:203091/1‑149 (MQ=255)
                                 cTTGAAGGCGAAATCTCAGACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGt                                                                                                                     >  1:722554/1‑149 (MQ=255)
                                      aGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTAc                                                                                                                <  1:689479/149‑1 (MQ=255)
                                       ggCGAAATCTCATACGATGACATGGTTTATCGTGGTGGTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACa                                                                                                               <  2:1146201/149‑1 (MQ=255)
                                       ggCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACa                                                                                                               <  1:1121171/149‑1 (MQ=255)
                                        gCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAg                                                                                                              >  2:102156/1‑149 (MQ=255)
                                        gCGAAATCTCAGACGATGAAATGGTTTAGCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGAAAACCTCGCTGCGTGGTTCGGAAGGGGTTCACTGGCTTGACAGTCAAAAACCAGAACAGGCGCGTGAAGAAGTATTACAg                                                                                                              >  1:604442/1‑149 (MQ=255)
                                           aaaTCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGgtt                                                                                                           <  1:730289/149‑1 (MQ=255)
                                             aTCTCATTCGTTGAAATGGTTTTTCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGgttgt                                                                                                         <  2:476607/149‑1 (MQ=255)
                                             aTCTCATACGATGAAATGGTTTATCGAGGGGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGGTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGgttgt                                                                                                         <  1:486801/149‑1 (MQ=255)
                                               ctcATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGttgttg                                                                                                       <  1:388179/149‑1 (MQ=255)
                                                     cGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTa                                                                                                 >  1:1149561/1‑149 (MQ=255)
                                                      gATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTAGGCGCAGCGGCAGATAACCTGGCTGAGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCTCGTGACGAAGTATTACAGGTTGTTGGTGCTaa                                                                                                >  1:267777/1‑148 (MQ=255)
                                                              ggTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCt                                                                                        <  1:171701/149‑1 (MQ=255)
                                                               gTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTg                                                                                       >  1:403116/1‑149 (MQ=255)
                                                               gTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTg                                                                                       >  2:22535/1‑149 (MQ=255)
                                                               gTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCAATGGCTTGCCAGTGAAAAACCAGCCCAGGCGCGTGACGAAGGATTACAGGTTCTTGGTGCTATCGCAGGATg                                                                                       >  1:661115/1‑149 (MQ=255)
                                                                tttATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGCGAAGGGGTTCACTGGCTTGACAGAGAAAAACCAGAACAGGCGAGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGa                                                                                      >  2:587199/1‑149 (MQ=255)
                                                                   aTCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAAtg                                                                                   >  1:76455/1‑149 (MQ=255)
                                                                    tCGAGGTGTGTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGCCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAAtgt                                                                                  <  2:905163/149‑1 (MQ=255)
                                                                        ggTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTAc                                                                              <  1:190748/149‑1 (MQ=255)
                                                                            tttGCGCCACGAGACAGTTGGCGATGCGGCAGATAACCTGGCTGCGGGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAAtt                                                                          >  1:313957/1‑149 (MQ=255)
                                                                            tttGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAAtt                                                                          >  2:887428/1‑149 (MQ=255)
                                                                            tttGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGCGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAAtt                                                                          <  1:1179429/149‑1 (MQ=255)
                                                                                 gcCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGAc                                                                     <  1:755766/149‑1 (MQ=255)
                                                                                     cGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGGATTACAGGTTGTTCGTGCGATCGCAGGCTGAATGTGTACTAATGAGACGTaa                                                                 >  2:486113/1‑148 (MQ=255)
                                                                                        gaCAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGt                                                              >  2:728366/1‑149 (MQ=255)
                                                                                           aGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTgcg                                                           >  1:922659/1‑149 (MQ=255)
                                                                                           aGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTgcg                                                           >  1:461188/1‑149 (MQ=255)
                                                                                                gCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGGTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAAtt                                                      <  2:299766/149‑1 (MQ=255)
                                                                                                     gCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTc                                                 <  2:716796/149‑1 (MQ=255)
                                                                                                     gCGGCAGATAACCTGGCTGCGTGGGTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTc                                                 <  1:932803/149‑1 (MQ=255)
                                                                                                      cGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGGGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCa                                                <  2:1101955/149‑1 (MQ=255)
                                                                                                       ggCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTGCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAATTATTACAGGTTGTTGGTGCTATCGCAGGCGGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAg                                               <  1:904763/149‑1 (MQ=255)
                                                                                                        gCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGAAGTATCGTGCGCAATTTTATCAGa                                              >  1:1154253/1‑149 (MQ=255)
                                                                                                        gCAGATAACCTGGCGGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACCGGCGCGTGACGAAGTATTACAGGGTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGa                                              <  2:675144/149‑1 (MQ=255)
                                                                                                           gATAACCTGGGTGCGTTGTTTGGAAGGGGTTCACTGGGTTGAAAGTGAAAAACCAGAACAGGCGCGTGACTAAGTATTACAGGTGGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGTCGTATCGTGCGCAATTTTTTCAGAATc                                           <  2:886793/149‑1 (MQ=255)
                                                                                                                  tGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCATGCTGAAAGTGTACTATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGt                                    >  1:1149347/1‑149 (MQ=255)
                                                                                                                    gCTGCGTGTTTGGGTTGGGGTTCTTTGGCTTGTCAGTGATAAACCAGTCGAGGCGCGTGACGATTTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTAGAATTGAGACGTTTCGTGCGTAATTTTTTCAGAATCGAAAGGTTc                                  <  1:817269/149‑1 (MQ=255)
                                                                                                                    gCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGtta                                  >  1:322203/1‑148 (MQ=255)
                                                                                                                      tGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTATTCAGAATCGAAAGGTTCaa                                >  1:785827/1‑149 (MQ=255)
                                                                                                                       gCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGTAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGCCGTATCGTGAGCAATTTTTTCAGAATCGATAGGTTCaaa                               >  1:848041/1‑149 (MQ=255)
                                                                                                                       gCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCaaa                               >  1:66729/1‑149 (MQ=255)
                                                                                                                         gTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAAATTTTTCAGAATCGATAGGTTCAAAGt                             >  2:493229/1‑149 (MQ=255)
                                                                                                                          tGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTa                            >  1:1165785/1‑149 (MQ=255)
                                                                                                                           ggTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTAc                           >  1:300140/1‑149 (MQ=255)
                                                                                                                            gTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACa                          >  1:570870/1‑149 (MQ=255)
                                                                                                                             ttGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACaa                         >  1:597006/1‑149 (MQ=255)
                                                                                                                               gggAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAAt                       <  2:896468/149‑1 (MQ=255)
                                                                                                                                 gAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCTCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTAAGAATCGAAAGGTTCAAAGTACAAATaa                     >  2:879613/1‑149 (MQ=255)
                                                                                                                                 gAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATaa                     >  1:344443/1‑149 (MQ=255)
                                                                                                                                 gAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATaa                     >  1:384394/1‑149 (MQ=255)
                                                                                                                                 gAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATaa                     <  1:3427/149‑1 (MQ=255)
                                                                                                                                    gtttttCACTGTCAAGCCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCa                  >  1:1006519/5‑149 (MQ=255)
                                                                                                                                    ggggTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCa                  >  1:715964/1‑149 (MQ=255)
                                                                                                                                     gggTTCACTGGCTTGTAAGTGAAAAACCAGAACAGGCGAGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCat                 <  2:267777/149‑1 (MQ=255)
                                                                                                                                     gggTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCGGAATGTGTACAATTGAGACGTATCGTGCGCAATGTTTTCAGAATCGAAATGTTCAAAGTACAAATAAGCat                 >  1:267321/1‑149 (MQ=255)
                                                                                                                                           aCTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACATGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAgg           <  2:891041/149‑1 (MQ=255)
                                                                                                                                           aCTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAgg           >  1:269851/1‑149 (MQ=255)
                                                                                                                                                cTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAag      >  1:528230/1‑149 (MQ=255)
                                                                                                                                                 ttGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAaga     <  2:66729/149‑1 (MQ=255)
                                                                                                                                                 ttGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAaga     <  1:887428/149‑1 (MQ=255)
                                                                                                                                                  tGACAGTGAAAAACCAGCACAGGAGCGTGACGAAGTATTACAGGGTGTTGGTTCTATCGCAGGCTGAATGTGTACAATTGAGACGATTCGTGCGCAATTTTTTCAGAATCGAAAGGGTCAAAGTACAAATAAGCATATAAGGAAAagag    <  1:883442/149‑1 (MQ=255)
                                                                                                                                                    aCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAagagag  >  2:364593/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTTGCGTGGGTTATCGCCAGATGTGGTCTTACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAAGCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAG  >  NC_000913/4399990‑4400286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: