Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 478,759 A→G 5.3% intergenic (‑142/+22) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913478,7590AG 5.3% 271.3 / 5.1 96intergenic (‑142/+22)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (53/36);  new base G (3/2);  total (56/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGC  >  NC_000913/478616‑478906
                                                                                                                                               |                                                                                                                                                   
aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCt                                                                                                                                                >  1:731666/1‑149 (MQ=255)
aCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAAAGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCt                                                                                                                                                >  1:666683/1‑149 (MQ=255)
 ccAGCTTATCCTTATTAACACAGTGTATGGAAAGTATATCTGATTGTGGATAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTGATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                                                                                               <  2:592261/149‑1 (MQ=255)
 ccAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg                                                                                                                                               <  2:310395/149‑1 (MQ=255)
  cAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTgg                                                                                                                                              <  1:508752/149‑1 (MQ=255)
   aGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGt                                                                                                                                             <  1:1033285/149‑1 (MQ=255)
    gCATATCCTTATTAACACAGTGTAGGGAAAGTATCTCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTa                                                                                                                                            <  2:631507/149‑1 (MQ=255)
       tatCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGCTGGAAACGATCATTGTCATTTGACCAGTCGCAGAGCTTTACGCCTGGTATGt                                                                                                                                         >  1:624034/1‑149 (MQ=255)
        atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtcaaat                                                                                                                                        <  1:1026424/149‑6 (MQ=255)
        atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                                                                                                        <  1:616375/149‑1 (MQ=255)
        atCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                                                                                                        <  2:465727/149‑1 (MQ=255)
          ccTTATTAACACAGTGTAGGGAAAGTATATCTGAGTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGATACGATCTTTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc                                                                                                                                      >  2:768307/1‑149 (MQ=255)
            ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                                                                                    <  1:216342/149‑1 (MQ=255)
            ttattaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGATGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTTGAAACGATAATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                                                                                    >  1:659242/1‑149 (MQ=255)
              attaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                                                                                  >  1:60228/1‑149 (MQ=255)
              attaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                                                                                  >  2:578113/1‑149 (MQ=255)
               ttaACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTaa                                                                                                                                 >  2:762572/1‑149 (MQ=255)
                  acacAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCGGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACtt                                                                                                                              <  2:136687/149‑1 (MQ=255)
                   cacaGTGTCGGGAAAGTATATATCATTGAGGAAAAAGGGTGGCTGGAGATAATGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTTTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                                                                                                             <  1:179655/149‑1 (MQ=255)
                   cacaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTa                                                                                                                             >  2:214092/1‑149 (MQ=255)
                    acaGTGTAGGGAAAGTATATCTGATTGTGGATAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                                                                                                            >  1:30444/1‑149 (MQ=255)
                    acaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                                                                                                            <  1:676273/149‑1 (MQ=255)
                    acaGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGAAGGGTGGAAACGATCATTGTCATTTGACCACGCGCAGAGCTATACGCCTGGTATGTAACCCTAACTTaa                                                                                                                            >  2:360393/1‑149 (MQ=255)
                        tgtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTACCCCTAACTTAAGATg                                                                                                                        >  2:697146/1‑149 (MQ=255)
                         gtAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGt                                                                                                                       <  1:869414/149‑1 (MQ=255)
                          tAGGGAAAGTATATCTGATTGTGGAAAAAGGGTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                                                                                      <  2:870328/149‑1 (MQ=255)
                          tAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                                                                                      >  1:213552/1‑149 (MQ=255)
                           aGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                                                                                                     >  2:717660/1‑149 (MQ=255)
                           aGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                                                                                                     >  1:1077738/1‑149 (MQ=255)
                              gAAAGTATATCTGATTGTGGAAAAAGCCTGGCTGGAGATCAGGAGGTAGAGCTGGTATTTAATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGCGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAtt                                                                                                                  <  1:1064026/149‑1 (MQ=255)
                               aaaGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGCGGTAGAGCTGGTATTTCATGAAGTGATGTGTGGAAACGATCATTGTCATTTGAACAGGCGCAGAGCTTTACGCATGTTATGTAACCCTAACTTAAGATTATGATTc                                                                                                                 >  1:739554/1‑149 (MQ=255)
                               aaaGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACACTAACTTAAGATGTTGATTc                                                                                                                 >  2:983591/1‑149 (MQ=255)
                                 aGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                                                                                                               <  1:403965/149‑1 (MQ=255)
                                          gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGATGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCTGCgcgagcga                                                                                                      >  1:319930/1‑149 (MQ=255)
                                          gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGATACGATCATAGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgccagcgc                                                                                                      >  2:861216/1‑148 (MQ=255)
                                          gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcga                                                                                                      >  2:197818/1‑149 (MQ=255)
                                          gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcga                                                                                                      >  1:328557/1‑149 (MQ=255)
                                            ttGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                                                                                                    <  1:214092/149‑1 (MQ=255)
                                            ttGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAgg                                                                                                    >  1:78006/1‑149 (MQ=255)
                                            ttGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTGGATTCGGCGCGAGCGAgg                                                                                                    <  1:1138/149‑1 (MQ=255)
                                                 tataaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGATGTGATGGGTGGAAACGATCTTTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaa                                                                                               <  2:624034/146‑1 (MQ=255)
                                                  aaaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa                                                                                              >  2:1152281/1‑149 (MQ=255)
                                                  aaaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa                                                                                              >  1:466987/1‑149 (MQ=255)
                                                  aaaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa                                                                                              >  1:44355/1‑149 (MQ=255)
                                                  aaaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaa                                                                                              >  2:526670/1‑149 (MQ=255)
                                                   aaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCGTAACTTAAGAGGTTGATTCGGCGCGAGCGAGGGTaaaaa                                                                                             >  2:183163/1‑149 (MQ=255)
                                                   aaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaaa                                                                                             >  1:253767/1‑149 (MQ=255)
                                                       ggctggctGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc                                                                                         <  2:544556/149‑1 (MQ=255)
                                                        gctggctgGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGTCGCGAGCGAGGTTAAAAATATCg                                                                                        >  2:327689/1‑149 (MQ=255)
                                                                gagaTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCGTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGa                                                                                >  1:613725/1‑149 (MQ=255)
                                                                 agaTAAGGACGTAGAGCTGGTATTTCATCAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCAGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGAc                                                                               <  1:983582/149‑1 (MQ=255)
                                                                   aTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGATGATTCGGCTCGAGCGAGGGTAAAAATATCGTAAAATGACAg                                                                             >  1:1149260/1‑149 (MQ=255)
                                                                       ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCa                                                                         >  2:982577/1‑149 (MQ=255)
                                                                       ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCa                                                                         >  1:214651/1‑149 (MQ=255)
                                                                       ggaggTAGAGCGGGTATTTCATGAAGTGATGTGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGTTGTTGATTCGGCGCGATCGAGGGTATAAATATCGTATAAATACAGTTCa                                                                         >  2:231454/1‑149 (MQ=255)
                                                                            tAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt                                                                    <  1:536308/149‑1 (MQ=255)
                                                                              gagCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc                                                                  >  1:789539/1‑149 (MQ=255)
                                                                                 cTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGa                                                               >  1:148738/1‑149 (MQ=255)
                                                                                 cTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGa                                                               >  2:254031/1‑149 (MQ=255)
                                                                                  tGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGTGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGAc                                                              <  2:1094427/149‑1 (MQ=255)
                                                                                             gAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCAtttttt                                                   >  2:355655/1‑149 (MQ=255)
                                                                                                   aTGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAgtt                                             <  1:1044127/149‑1 (MQ=255)
                                                                                                       gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAACAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTc                                         <  1:143023/149‑1 (MQ=255)
                                                                                                       gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTc                                         <  2:1173087/149‑1 (MQ=255)
                                                                                                       gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTc                                         <  2:108273/149‑1 (MQ=255)
                                                                                                       gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTc                                         <  2:1077738/149‑1 (MQ=255)
                                                                                                        tGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTcc                                        <  2:425834/149‑1 (MQ=255)
                                                                                                        tGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTcc                                        >  1:102642/1‑149 (MQ=255)
                                                                                                        tGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTcc                                        >  2:152002/1‑149 (MQ=255)
                                                                                                         ggAAAAGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAATACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCt                                       <  2:1145054/149‑1 (MQ=255)
                                                                                                          gAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCtt                                      >  2:337413/1‑149 (MQ=255)
                                                                                                          gAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCtt                                      >  1:757217/1‑149 (MQ=255)
                                                                                                           aaaCGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTa                                     >  1:57846/1‑149 (MQ=255)
                                                                                                            aaCGATCATTGTGATTTGACCAGGCGCAGAGCTTTTCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTAt                                    <  2:1177704/149‑1 (MQ=255)
                                                                                                             aCGATCATTGTCATTTGACCAGGCGCAGAGCTTTACTCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATg                                   <  1:578113/149‑1 (MQ=255)
                                                                                                              cGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGa                                  <  1:1152281/149‑1 (MQ=255)
                                                                                                               gATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGaa                                 >  2:660457/1‑149 (MQ=255)
                                                                                                                         aTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGac                       >  2:206235/1‑149 (MQ=255)
                                                                                                                         aTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGac                       >  2:702851/1‑149 (MQ=255)
                                                                                                                         aTTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGac                       >  1:84660/1‑149 (MQ=255)
                                                                                                                            ataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACAcg                    >  1:583006/3‑149 (MQ=255)
                                                                                                                              cccAGGCGCAGCGCTTTCCGCCTGGTATGTAACCCTAACATCAGCTGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTACAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACAcgcg                  <  1:768307/148‑1 (MQ=255)
                                                                                                                              aCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACAcgcg                  >  2:429906/1‑149 (MQ=255)
                                                                                                                              aCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACAcgcg                  >  1:467463/1‑149 (MQ=255)
                                                                                                                              aCCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACAcgcg                  >  1:450432/1‑149 (MQ=255)
                                                                                                                               ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGCCGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCgg                 >  2:1019716/1‑149 (MQ=255)
                                                                                                                               ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCgg                 >  1:127504/1‑149 (MQ=255)
                                                                                                                                cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGga                >  1:976475/1‑149 (MQ=255)
                                                                                                                                cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGga                >  2:106499/1‑149 (MQ=255)
                                                                                                                                cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGga                <  1:254031/149‑1 (MQ=255)
                                                                                                                                   gcgcAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGagag             <  2:466987/149‑1 (MQ=255)
                                                                                                                                      cAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTc          <  2:731666/149‑1 (MQ=255)
                                                                                                                                       agagCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCa         <  1:736601/149‑1 (MQ=255)
                                                                                                                                            tttACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATg    <  1:1107656/149‑1 (MQ=255)
                                                                                                                                             ttACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGATCACCTTTACGGACGATCATTTTTAGCAGTTGTTCCTTATGAACATCCGCGACCCGCGGAGAGTTCAAGATgg   >  1:1099877/1‑149 (MQ=255)
                                                                                                                                              tACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGc  <  1:429906/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGC  >  NC_000913/478616‑478906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑