Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	715,756	G→T	11.3%	Q115H (CAG→CAT) ‡	ybfP →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,756	0	G	T	11.3%	182.0 / 10.0	75	Q115H (CAG→CAT) ‡	ybfP	lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (39/27);  new base T (5/4);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAT  >  NC_000913/715610‑715903                                                                                                                                                   |                                                                                                                                                    aaCTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAggg                                                                                                                                                   <  2:263500/149‑1 (MQ=255)   cTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCa                                                                                                                                                 >  1:110142/1‑149 (MQ=255)      aCCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGTGCAAGc                                                                                                                                              >  2:1124722/1‑149 (MQ=255)        cTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGccc                                                                                                                                            >  1:327555/1‑149 (MQ=255)         tGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAACAGAACTTACTTATCTTCATATTATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGCTTGCCCTGGCAAACCCg                                                                                                                                           <  1:63150/149‑1 (MQ=255)         tGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCg                                                                                                                                           >  2:733781/1‑149 (MQ=255)           ataataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGCTTGCCCTGGCAAACCCgca                                                                                                                                         <  2:323941/149‑3 (MQ=255)                aCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCg                                                                                                                                    >  1:689931/1‑149 (MQ=255)                 cccGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                                   >  1:1131040/1‑149 (MQ=255)                 cccGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGa                                                                                                                                   <  2:1000511/149‑1 (MQ=255)                  ccgccgCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCCCAAGAAAAGGTCGGTTTAAATCCCGGATGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCTGCTCTCGAc                                                                                                                                  >  1:767041/1‑149 (MQ=255)                  ccgccgCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGAc                                                                                                                                  >  2:4678/1‑149 (MQ=255)                           gCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCa                                                                                                                         >  1:322099/1‑149 (MQ=255)                             aGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAATAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTGTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAAt                                                                                                                       <  2:737019/149‑1 (MQ=255)                             aGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTAAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGCGTTTGCCAGGGCAAGCCCGGCAATCGACCGAAAGTCaaa                                                                                                                       >  1:1057916/1‑148 (MQ=255)                              gTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGGTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCCAGCACGGCACTCGACCGATAGTCAAtt                                                                                                                      >  1:481513/1‑148 (MQ=255)                                 gTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAgg                                                                                                                   >  2:137625/1‑149 (MQ=255)                                    aGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGCCCGATAGTCAATGAGGAtt                                                                                                                >  2:151248/1‑149 (MQ=255)                                      gCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTAt                                                                                                              >  2:45950/1‑149 (MQ=255)                                       cGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGGCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATg                                                                                                             <  1:527168/149‑1 (MQ=255)                                              gAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTAAACCTCATTTTTTATGCGGGTTTGCCATTGCAAGCACGGAACTCGACCGATATTCAATGAGGATTATGGCAGCGa                                                                                                      >  2:840288/1‑149 (MQ=255)                                                aaatGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGaaa                                                                                                    <  1:817085/149‑1 (MQ=255)                                                aaaaGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGaaa                                                                                                    <  1:1055267/149‑1 (MQ=255)                                                 aaaGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAg                                                                                                   >  2:243046/1‑149 (MQ=255)                                                  aaGAACTTACTTATCATCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGc                                                                                                  <  1:21475/149‑1 (MQ=255)                                                            ttaTCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGTTTTGCCAGGGCAAGCCCGGCCCTCGACCGATAGTCAATGAGGATTATGGAAGCGAAAGCGGAATTTTTg                                                                                        >  2:58676/1‑149 (MQ=255)                                                            ttaTCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCAGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTg                                                                                        >  2:739823/1‑149 (MQ=255)                                                                  tCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  <  1:1170364/149‑1 (MQ=255)                                                                  tCATAATATGTCAGCACAAGAAAAGGGCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCga                                                                                  <  2:462297/149‑1 (MQ=255)                                                                    ataataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTAAGCGAAAGCGGATTTTTTGGCTCgagt                                                                                >  1:573629/1‑147 (MQ=255)                                                                     taataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatga                                                                               >  1:200721/1‑149 (MQ=255)                                                                      aataTGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCgatgat                                                                              <  1:733781/149‑1 (MQ=255)                                                                       atatGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTGCTCGatgatg                                                                             >  2:1098103/1‑149 (MQ=255)                                                                          tGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCAt                                                                          >  1:411821/1‑149 (MQ=255)                                                                            tCAGCTCAAGAAAAGGTCGGTTTAAATCCAGGTTGGACATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGTCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGt                                                                        <  1:207893/149‑1 (MQ=255)                                                                               gCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCgg                                                                     >  2:1015340/1‑149 (MQ=255)                                                                                  caAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGat                                                                  <  1:341577/149‑1 (MQ=255)                                                                                  caAGAAAACGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTATGCCAGCGCAAGCCAGGCACTCGACCGAAAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGAAGATGCATGTCGGGat                                                                  >  1:352477/1‑149 (MQ=255)                                                                                     gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGattat                                                               >  1:1122896/1‑149 (MQ=255)                                                                                     gAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGAAGCATGTCGGGattat                                                               >  1:348554/1‑149 (MQ=255)                                                                                       aaaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTg                                                             <  1:603119/149‑1 (MQ=255)                                                                                        aaGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGa                                                            >  1:532620/1‑149 (MQ=255)                                                                                                   aaaTCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCt                                                 >  2:526975/1‑149 (MQ=255)                                                                                                       cccGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCa                                             >  2:77356/1‑149 (MQ=255)                                                                                                         cGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGt                                           <  1:1124722/149‑1 (MQ=255)                                                                                                         cGGTTGGCAATGCTACACCTCATTTTATATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGt                                           <  2:406789/149‑1 (MQ=255)                                                                                                           gTTGGCAATGCTACACCTCATTTTTTATTCGGGTATGCCAGGGAAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTTTTGAGTTACGACGCTGCCAGTCa                                         <  1:694336/149‑1 (MQ=255)                                                                                                            ttGGCAATGCTACACCTCATTTTTTATGCGGGTTTGACATGGCAAGCCCGGGACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAg                                        <  1:155191/149‑1 (MQ=255)                                                                                                                 aaTGCTACACCTCATTTTTTATGCGGGTTTGACATTGCAAGCCCGGCACTCGACAGATAGACAATGATGATTATGTAAGCTAAAGCGGATTTTTTGGATCGATGATGCATGTCGGGAATATTGAGTTACGACGCTGCCAGTCAGAAAAt                                   >  2:697981/1‑149 (MQ=255)                                                                                                                  aTGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAAtt                                  <  1:85508/149‑1 (MQ=255)                                                                                                                    gCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAACGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGc                                >  2:1058852/1‑149 (MQ=255)                                                                                                                      tACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCa                              <  2:1131040/149‑1 (MQ=255)                                                                                                                      tACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCa                              >  2:302053/1‑149 (MQ=255)                                                                                                                          ccTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCaa                          <  1:137625/149‑1 (MQ=255)                                                                                                                            tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGCCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                        >  1:1008889/1‑149 (MQ=255)                                                                                                                            tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                        <  2:689931/149‑1 (MQ=255)                                                                                                                            tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                        >  2:980782/1‑149 (MQ=255)                                                                                                                            tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                        >  1:491935/1‑149 (MQ=255)                                                                                                                            tCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAAGGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGGCGGGAGTTTTGGGTTACGCCGCGGCCAGTCAGCAAATTGccggaatca                        >  2:217884/1‑142 (MQ=255)                                                                                                                             cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCTATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                       <  2:926595/149‑1 (MQ=255)                                                                                                                             cATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                       <  1:77356/149‑1 (MQ=255)                                                                                                                                tttttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                    <  1:45950/149‑1 (MQ=255)                                                                                                                                  tttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                  <  2:322099/149‑1 (MQ=255)                                                                                                                                   ttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                 <  2:532620/149‑1 (MQ=255)                                                                                                                                   ttATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                 >  1:1005352/1‑149 (MQ=255)                                                                                                                                     tgccGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGCATATTTTGGCACGATGATGCATGTCGGGATTATTGAGTTACGACGCTGACAGTCAGAAAAATGCCAGCAAGAATTGAAAGc               >  2:490918/4‑149 (MQ=255)                                                                                                                                      tGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg              <  1:753870/149‑1 (MQ=255)                                                                                                                                       ccGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGa             >  1:481823/2‑149 (MQ=255)                                                                                                                                        cGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                     >  1:940374/1‑140 (MQ=255)                                                                                                                                        cGGGCTTGCCCTGGCAAACCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAAtt                     <  2:940374/140‑1 (MQ=255)                                                                                                                                         gggTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATa           >  1:271669/1‑149 (MQ=255)                                                                                                                                               gCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca     >  1:675617/1‑149 (MQ=255)                                                                                                                                                ccAGGGCAAGCCCGGGACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTAGGGATTATTGAGTTACGACGCTGCGAGTCAGAAATTTGCCAGCAAGAATTGAAAGCGATAAATacac    <  1:227889/149‑1 (MQ=255)                                                                                                                                                ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac    <  2:200721/149‑1 (MQ=255)                                                                                                                                                ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac    >  1:1175151/1‑149 (MQ=255)                                                                                                                                                ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac    >  1:1144214/1‑149 (MQ=255)                                                                                                                                                  cgggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAt  <  2:78415/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCCCGGTTGGCAATGCTACACCTCATTTTTTATGCGGGTTTGCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACAT  >  NC_000913/715610‑715903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑