Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 14,129 T→C 8.7% intergenic (+50/‑39) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,1290TC 8.7% 255.4 / 12.8 92intergenic (+50/‑39)dnaK/dnaJchaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base T (40/44);  new base C (3/5);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

GCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGT  >  NC_000913/13981‑14275
                                                                                                                                                    |                                                                                                                                                  
gCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCct                                                                                                                                                    >  2:419303/1‑149 (MQ=255)
      cagctgACTGCCTGTGCTGATGCTTCTGCAAACAACGCGATAGATGACGATGTTGGCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGc                                                                                                                                              <  1:548992/149‑1 (MQ=255)
       agcagACTGCCGGTGCTTATGCTTCAGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAAAAATCGCCCTATAAACGGGTAAATATACTGACACGGGCGGAGAGGAAATTCCCCTTCGcc                                                                                                                                             <  2:1066817/149‑1 (MQ=255)
        ccagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGAGGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGccc                                                                                                                                            <  1:1088900/148‑1 (MQ=255)
         cagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCCTCTCCGCCCg                                                                                                                                           >  2:120117/1‑149 (MQ=255)
          agACTGCCGTTGCCGATGCTTCTGCAAACAACGCGAAAGATGACGATGGCGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTCATTATCCTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGt                                                                                                                                          <  1:1177769/149‑1 (MQ=255)
            aCTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTATTTATACTGACACGGGCGAAGGGGAATTTCATCTCCTCCCGtcc                                                                                                                                        >  1:791268/1‑147 (MQ=255)
              tGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAt                                                                                                                                      >  1:755644/1‑149 (MQ=255)
                      cTGATGCTTCTGCAAACAACGCTAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATATCCTCTCCGCCCGTGCATTCATCTAg                                                                                                                              <  1:910689/149‑1 (MQ=255)
                         aTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAgggg                                                                                                                           >  2:1001044/1‑149 (MQ=255)
                             ttCTGCAAACAACGCGAAAGCTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                                       >  1:176426/1‑149 (MQ=255)
                             ttCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                                       >  1:1117416/1‑149 (MQ=255)
                                 gCAAACAACGCGAATGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAACGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGCGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCTATTTaa                                                                                                                   >  1:781892/1‑149 (MQ=255)
                                   aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                                 <  1:609637/149‑1 (MQ=255)
                                   aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                                 <  1:1058821/149‑1 (MQ=255)
                                     acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                               >  1:303851/1‑149 (MQ=255)
                                     acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                               >  2:363032/1‑149 (MQ=255)
                                       aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                             <  2:723094/149‑1 (MQ=255)
                                       aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                             >  1:873460/1‑149 (MQ=255)
                                        acGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                                                                            <  1:362852/149‑1 (MQ=255)
                                            gAAATAAGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGCCAATAAATATTGGCCCTATAAACGGTTTATTATACTGACAAGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTATAAATGATGGCt                                                                                                        <  1:240475/149‑1 (MQ=255)
                                            gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCt                                                                                                        >  1:942262/1‑149 (MQ=255)
                                            gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCATCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCt                                                                                                        >  1:307302/1‑149 (MQ=255)
                                              aaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTaa                                                                                                      <  1:788177/149‑1 (MQ=255)
                                               aGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAg                                                                                                     >  2:778669/1‑149 (MQ=255)
                                                gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTGTAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                                    <  1:372139/149‑1 (MQ=255)
                                                gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                                    <  1:127057/149‑1 (MQ=255)
                                                   gACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                                                                                 >  2:204730/1‑149 (MQ=255)
                                                     cGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat                                                                                               >  2:603976/1‑149 (MQ=255)
                                                      gATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGatt                                                                                              >  2:1065607/1‑149 (MQ=255)
                                                         gttgtCGACGCTGTATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAAGGGGTAATTATAGTGACTCGGGAGTAGGTGAATTTCCTCTCCGCCCGTGCATTCATCTATTGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                                           <  2:526371/149‑1 (MQ=255)
                                                         gttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                                           <  1:1127813/149‑1 (MQ=255)
                                                          ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACTGGTAATTATACTGACCCGGGCGAAGGGGAATTTCCTCTCCGCCGGTGCATTCTTCTAGGGGCAATTTAAAAAAGATGGGTAAGCAAGAttatta                                                                                          <  2:995438/149‑1 (MQ=255)
                                                           tgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTAc                                                                                         <  1:224451/149‑1 (MQ=255)
                                                            gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTAATCTAGGGCAAATTTAAAAAAGATGGCTAAGCATGTTTATTAcc                                                                                        >  2:660934/1‑148 (MQ=255)
                                                            gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACg                                                                                        >  1:21593/1‑149 (MQ=255)
                                                               gACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga                                                                                     >  1:1177044/1‑149 (MQ=255)
                                                                aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAt                                                                                    >  1:261301/1‑149 (MQ=255)
                                                                   atcaaTTTCCAGAAGTCAATGAAAAAAAATAATCGCCCTAAAAACGGCTAATTATACTTACAAGTGCGAAGGGGAATTTCCTCTACGCCCGTGCATTCATCTAGTTGAAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAtttt                                                                                 <  2:768308/146‑1 (MQ=255)
                                                                    tGAATTTGAAGAAGTCAAAGCCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa                                                                                >  2:526896/1‑149 (MQ=255)
                                                                      aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                              <  1:452322/149‑1 (MQ=255)
                                                                      aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                              <  2:596181/149‑1 (MQ=255)
                                                                        tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                                      >  2:1132508/1‑88 (MQ=25)
                                                                        tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                                      <  1:1132508/91‑4 (MQ=25)
                                                                          tGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGtt                                                                          >  2:350661/1‑149 (MQ=255)
                                                                           gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                                         <  1:947493/149‑1 (MQ=255)
                                                                           gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                                         >  1:494028/1‑149 (MQ=255)
                                                                           gaagaaGTCAAAGACAAAAAATAATCGCACTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCtttt                                                                         >  2:302551/1‑149 (MQ=255)
                                                                            aagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc                                                                        <  1:172104/149‑1 (MQ=255)
                                                                               aagTCAAACACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                                                                     <  2:568609/149‑1 (MQ=255)
                                                                                 gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                                   >  1:75820/1‑149 (MQ=255)
                                                                                 gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                                   <  1:290868/149‑1 (MQ=255)
                                                                                   cAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                                 >  1:389424/1‑149 (MQ=255)
                                                                                   cAAAGACAAAAAATAAGCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAGGGCTACGCCAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                                 <  1:206645/149‑1 (MQ=255)
                                                                                    aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg                                                                <  2:656595/149‑1 (MQ=255)
                                                                                    aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACGGACACCGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATACTTCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACTAGAGTTTAGGATTTTCCAAAACat                                                                >  1:683795/1‑148 (MQ=255)
                                                                                      aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCg                                                              <  1:1109858/149‑1 (MQ=255)
                                                                                       gACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg                                                             <  1:1001044/149‑1 (MQ=255)
                                                                                        aCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGTCGTTTCCACAACAGCGGa                                                            >  1:178601/1‑149 (MQ=255)
                                                                                         cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTATCCAAAACAGCGGaa                                                           >  1:839428/1‑149 (MQ=255)
                                                                                           aaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAaga                                                         <  2:923239/149‑1 (MQ=255)
                                                                                            aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                                        >  2:418532/1‑148 (MQ=255)
                                                                                            aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAGTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagag                                                        >  2:889523/1‑149 (MQ=255)
                                                                                            aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAATATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                                        >  1:1172851/1‑148 (MQ=255)
                                                                                               ataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGt                                                     <  1:500939/149‑1 (MQ=255)
                                                                                                  atCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGaa                                                  <  1:204730/149‑1 (MQ=255)
                                                                                                    cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt                                                <  1:363653/149‑1 (MQ=255)
                                                                                                     tcccTATAAACGGGTAATTATATTGACACGGGCGAAGGTGAATTTCCTCTCAGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATc                                               <  2:247476/148‑1 (MQ=255)
                                                                                                         tataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCTAGATTATTACTAGATTTTAGGAGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaa                                           >  1:59239/1‑149 (MQ=255)
                                                                                                          ataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaaa                                          >  1:1177249/1‑149 (MQ=255)
                                                                                                              aCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTTTTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGTcc                                      >  1:1117218/1‑149 (MQ=255)
                                                                                                               cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCt                                     <  1:724384/149‑1 (MQ=255)
                                                                                                                gggTAATTATACTGACACGGGCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    <  2:1163392/149‑1 (MQ=255)
                                                                                                                gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    >  1:961842/1‑149 (MQ=255)
                                                                                                                gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    <  1:937262/149‑1 (MQ=255)
                                                                                                                 ggTAACTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTAc                                   <  1:550521/149‑1 (MQ=255)
                                                                                                                  gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACa                                  >  1:38847/1‑149 (MQ=255)
                                                                                                                       tataCTGACACTGGCCTAGGGGAATTTCCTCTCCGCGCGTGCATTCATCTAGGCGCACTTTAAAAAAGCTGGCTTAGCAAGATTTTTACGAGATTTTCGGCGTTTCCAACACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                             <  2:898136/149‑1 (MQ=255)
                                                                                                                       tataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                             <  1:412355/149‑1 (MQ=255)
                                                                                                                                acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg                    >  2:503152/1‑149 (MQ=255)
                                                                                                                                acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg                    >  2:507573/1‑149 (MQ=255)
                                                                                                                                 cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGa                   <  2:746640/149‑1 (MQ=255)
                                                                                                                                  gggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGaa                  <  2:791268/149‑1 (MQ=255)
                                                                                                                                    gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAAt                >  2:447900/1‑149 (MQ=255)
                                                                                                                                     cGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATa               <  1:611222/149‑1 (MQ=255)
                                                                                                                                      gAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATac              <  1:350661/149‑1 (MQ=255)
                                                                                                                                           gtaaTTTCCTCACAGCCAGGTCATTCATCTAGGGGCAATTTAAAAAACATGGCTAAGCAAGATTATTAGGATATCTTAGGCGTTTGCAAAACAGCGTAAGAGCGTGAAATCAGAATGGCCTTCAAACGCCTGGCCATGAAATAACAccc         <  2:188329/147‑1 (MQ=255)
                                                                                                                                            gAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCg        >  1:1053400/1‑149 (MQ=255)
                                                                                                                                              aattCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  1:503152/147‑1 (MQ=255)
                                                                                                                                              aattCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  1:507573/147‑1 (MQ=255)
                                                                                                                                              aTTTCCTCTCCGCCCGTGCATTAATCTAGGTGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  2:839428/149‑1 (MQ=255)
                                                                                                                                                ttCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGAcc    <  1:140525/149‑1 (MQ=255)
                                                                                                                                                  ccTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGt  >  1:467224/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGT  >  NC_000913/13981‑14275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: