Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,201 T→G 5.5% K125N (AAA→AAC)  artJ ← arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,2010TG 5.5% 188.0 / 2.4 73K125N (AAA→AAC) artJarginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (32/36);  new base G (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

TCACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCA  >  NC_000913/900055‑900349
                                                                                                                                                  |                                                                                                                                                    
tCACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCGGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCttt                                                                                                                                                    <  2:532068/149‑1 (MQ=255)
 cACCAAATACCCGATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGATACAGTTTTCACTTCCTGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTc                                                                                                                                                   <  1:862853/149‑1 (MQ=255)
  aCCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCa                                                                                                                                                  <  1:1094831/149‑1 (MQ=255)
        tACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                            <  2:923151/149‑1 (MQ=255)
        tACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                            <  2:939577/149‑1 (MQ=255)
           cccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaa                                                                                                                                         >  2:511793/1‑149 (MQ=255)
           cccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaa                                                                                                                                         >  2:441722/1‑149 (MQ=255)
            ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                        <  1:511793/149‑1 (MQ=255)
            ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                        >  1:570762/1‑149 (MQ=255)
            ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCaaa                                                                                                                                        <  1:511585/149‑1 (MQ=255)
            ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCAGAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTGTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                        >  2:814030/1‑149 (MQ=255)
              aTCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACg                                                                                                                                      >  1:1180586/1‑149 (MQ=255)
              aTCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACg                                                                                                                                      <  2:422526/149‑1 (MQ=255)
                cAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGtt                                                                                                                                    <  2:292221/149‑1 (MQ=255)
                   tACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTg                                                                                                                                 >  1:113005/1‑149 (MQ=255)
                        ccATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCATTCAGATCGGCAAACGTTTTGTAGGt                                                                                                                            >  1:229293/1‑149 (MQ=255)
                         cATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTa                                                                                                                           >  1:516539/1‑149 (MQ=255)
                              ttCAGATCGATATAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCTGGTGCTGTTCCTGAATATATTTCTTGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCATATCGGCAAACGTTTTGTAGGTATCttt                                                                                                                      <  1:953111/149‑1 (MQ=255)
                                cAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGGGCTGATCCTGAATATATTTCTGGTGCGTACTACCGTTTTCCATCCCAATCCGTGTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCttttt                                                                                                                    <  1:861007/149‑1 (MQ=255)
                                 agatagatAAAGGCATTCTGATAACTTTCATAAGAGACAGTTTTCACTTCAGGGTGATGAACCTGAATATATTTCTGGTGCCTACTACCGTTTTCCATCCAAATACGTTTGCCTTTCAGATAGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                   >  1:586137/1‑149 (MQ=255)
                                 aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                   <  2:905167/149‑1 (MQ=255)
                                 aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                   >  1:811569/1‑149 (MQ=255)
                                 aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                   <  2:241024/149‑1 (MQ=255)
                                  gATCGATAAAGGCATTCTGATAACTTTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTg                                                                                                                  <  2:875078/149‑1 (MQ=255)
                                  gATCGAAAAAGGCATTCTTATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCCTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTg                                                                                                                  <  2:66958/149‑1 (MQ=255)
                                   ttCGATAAAGGCTTTCTGATATCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                 <  2:604885/148‑1 (MQ=255)
                                   aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                 >  1:310210/1‑149 (MQ=255)
                                   aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                 >  1:1150556/1‑149 (MQ=255)
                                               aTTCTGATAACTGTCATAAGAGACAGTTTTGACTTCCGGGAGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTAACTTTTTTTGCAATCAAGACgg                                                                                                     >  2:361148/1‑149 (MQ=255)
                                                ttCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                    <  1:441722/149‑1 (MQ=255)
                                                ttCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                    >  2:335707/1‑149 (MQ=255)
                                                   tGATAACTTTGATTAGATACAGTTTTCACTTGAGTGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGTTCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCTCGACGGCTGa                                                                                                 <  1:654871/149‑1 (MQ=255)
                                                   tGATAACTGTCATAAGAGACAGTTTTGACTTGCGGTTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCGTATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGa                                                                                                 <  1:524437/149‑1 (MQ=255)
                                                    gATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAg                                                                                                >  2:714650/1‑149 (MQ=255)
                                                     aTAACTGTCATAAGAGACAGTTTTCCCTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGt                                                                                               >  1:998614/1‑149 (MQ=255)
                                                         atggcATAAGAGACAGTTTTCACTTACGGGTGCTGATACTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTc                                                                                           <  2:863929/145‑1 (MQ=255)
                                                              aTAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtagt                                                                                      <  2:941994/149‑1 (MQ=255)
                                                                  gagaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAggg                                                                                  >  1:929721/1‑149 (MQ=255)
                                                                     aCAGTTTTCACTTGCGGGTGCTGAGCCTTAATATTTTTCTGGTGCGTAGTACCGTTTTCCATCCCACTACGTTTGCCTTTCAGATCGGCTAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                               <  1:573463/149‑1 (MQ=255)
                                                                         ttttCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTa                                                                           >  1:462112/1‑149 (MQ=255)
                                                                          tttCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTaa                                                                          >  2:312874/1‑149 (MQ=255)
                                                                           ttaaattCCGGGTGCGGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTACATACCAATACGTTTGCCTTTACGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTaaa                                                                         <  1:624548/144‑1 (MQ=255)
                                                                            tCACTTCCGGGTGCTGATCATGAATATATTTCTGGTGCGTAGTACCGCTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAAc                                                                        <  2:986088/149‑1 (MQ=255)
                                                                                      gTGCTGATCCTGAATATATTTCTGGGGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGtt                                                              <  1:440217/149‑1 (MQ=255)
                                                                                       tgctgcACCTGAATATATTGATGGCGCGTAGTACCGGATTGCATCCCAATACGTTTGCCTTTCAGATCGGCAAACTTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGCGCGTGGTAAACGAAACCTGttt                                                             <  2:939902/149‑1 (MQ=255)
                                                                                       tGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGttt                                                             >  1:355801/1‑149 (MQ=255)
                                                                                        gCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTg                                                            <  1:241307/149‑1 (MQ=255)
                                                                                         cTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTgg                                                           >  1:352861/1‑148 (MQ=255)
                                                                                          tGATCCTGAATATATTTCTGGTGCGTAGTACCCTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGCCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCt                                                          >  1:590197/1‑149 (MQ=255)
                                                                                           gacccTGAATATAATGTTTGTGCGTCGTCCCGCTTTCCCTCAAAATAATTTCGAATTTAAGATCGTCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                         <  2:1186280/146‑1 (MQ=255)
                                                                                           gATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                         >  1:899415/1‑149 (MQ=255)
                                                                                           gATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                         <  1:244802/149‑1 (MQ=255)
                                                                                            aTCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTAc                                                        >  1:971159/1‑149 (MQ=255)
                                                                                               cTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCt                                                     <  2:141448/149‑1 (MQ=255)
                                                                                                  aaTATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                  <  2:516539/149‑1 (MQ=255)
                                                                                                  aaTATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                  <  1:79668/149‑1 (MQ=255)
                                                                                                    tatatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACCGCTGAGTTTACATAGTAGGGCGTGGTAACCGCTCCCTGTTTGCTACGCTCCggg                                                >  2:698278/1‑149 (MQ=255)
                                                                                                      tatTTCTGGTGCGTAGTACCGTGTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGt                                              >  2:30712/1‑149 (MQ=255)
                                                                                                       atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                             >  1:464225/1‑149 (MQ=255)
                                                                                                       atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTGCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGCCGGCTGAGTTTTCATAGGAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                             <  1:587333/149‑1 (MQ=255)
                                                                                                           cTGGGGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGatat                                         <  2:229293/149‑1 (MQ=255)
                                                                                                              gTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTCTCTTTTATGGCAAACACGACGGCTGAGGTTTCATAGTAGGGCGTGGTAAAAGATACCTGTTTGCTACGCTCCGGGGTGATATCCa                                      >  1:585864/1‑149 (MQ=255)
                                                                                                                 cGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATAc                                   <  2:629802/149‑1 (MQ=255)
                                                                                                                     ttaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                               <  2:1160366/148‑1 (MQ=255)
                                                                                                                     gtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                               <  1:335707/149‑1 (MQ=255)
                                                                                                                      taCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGgag                              >  1:1097606/1‑149 (MQ=255)
                                                                                                                        ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGAGTGGTAATCTATACCTGTTTGCTACGCTCCGGGGTGATATCCATAAAGGAGAt                            >  1:79454/1‑149 (MQ=255)
                                                                                                                         cGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTGGGGAATCACGACGGCTGAGTGTTCATAGTAGGGGGTGGTAAGCGATTCCTGTTTGCTTCGCTCAGGGGTGATATCAATACCGGAGAtt                           >  2:686844/1‑149 (MQ=255)
                                                                                                                              tCCATCCCAATACGTTTGCCTTTCAGATCCGCAAACGTTTTGTAGGTATCTTTTTTGGCTATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGTGGTGATATCCATACCGGAGATTACGGc                      <  2:871005/149‑1 (MQ=255)
                                                                                                                               ccATCCCAATACGTTTGCCTTTCAGATCGGCTAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAATTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGCGGTGATATACATACCGGAGATTACGGCg                     >  1:668016/1‑149 (MQ=255)
                                                                                                                                    acAAAACGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCatt                >  1:600784/2‑148 (MQ=255)
                                                                                                                                     cAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATACATACCGGAGATTAAGGCGTCatat               >  1:1079676/1‑149 (MQ=255)
                                                                                                                                        aaCGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATGCATACCGGAGATTACGGCGTCATAtttt            >  1:1026076/2‑149 (MQ=255)
                                                                                                                                         aCGTTTGCCTTTCAGATCGGCAATCGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTc           <  1:361148/149‑1 (MQ=255)
                                                                                                                                            tttgaatttCAGATCGGGAAACGTTTTGTAGGTATCTTTTTAGGCAATCACAACGGCTGAGCTTTCAAAGTAGGGCGTCGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGa        <  2:60514/143‑1 (MQ=255)
                                                                                                                                                  tttCAGATCGGCAAACGTTTTGTATGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTATGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATACATACCGGAGATTACGGCGTCATATTTTGTGACTTTCa  >  2:688114/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCACCAAATACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCA  >  NC_000913/900055‑900349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑