Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,209 C→A 5.8% D123Y (GAT→TAT)  artJ ← arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,2090CA 5.8% 192.1 / 2.5 77D123Y (GAT→TAT) artJarginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base A (3/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

TACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGG  >  NC_000913/900063‑900356
                                                                                                                                                  |                                                                                                                                                   
tACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                                   <  2:939577/149‑1 (MQ=255)
tACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCgg                                                                                                                                                   <  2:923151/149‑1 (MQ=255)
   cccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaa                                                                                                                                                >  2:441722/1‑149 (MQ=255)
   cccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaa                                                                                                                                                >  2:511793/1‑149 (MQ=255)
    ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                               >  1:570762/1‑149 (MQ=255)
    ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                               <  1:511793/149‑1 (MQ=255)
    ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCaaa                                                                                                                                               <  1:511585/149‑1 (MQ=255)
    ccATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCAGAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTGTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCaaa                                                                                                                                               >  2:814030/1‑149 (MQ=255)
      aTCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACg                                                                                                                                             >  1:1180586/1‑149 (MQ=255)
      aTCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCGATCTGAAAGGCAAACg                                                                                                                                             <  2:422526/149‑1 (MQ=255)
        cAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGtt                                                                                                                                           <  2:292221/149‑1 (MQ=255)
           tACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTg                                                                                                                                        >  1:113005/1‑149 (MQ=255)
                ccATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCATTCAGATCGGCAAACGTTTTGTAGGt                                                                                                                                   >  1:229293/1‑149 (MQ=255)
                 cATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTa                                                                                                                                  >  1:516539/1‑149 (MQ=255)
                      ttCAGATCGATATAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCTGGTGCTGTTCCTGAATATATTTCTTGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCATATCGGCAAACGTTTTGTAGGTATCttt                                                                                                                             <  1:953111/149‑1 (MQ=255)
                        cAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGGGCTGATCCTGAATATATTTCTGGTGCGTACTACCGTTTTCCATCCCAATCCGTGTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCttttt                                                                                                                           <  1:861007/149‑1 (MQ=255)
                         agatagatAAAGGCATTCTGATAACTTTCATAAGAGACAGTTTTCACTTCAGGGTGATGAACCTGAATATATTTCTGGTGCCTACTACCGTTTTCCATCCAAATACGTTTGCCTTTCAGATAGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                          >  1:586137/1‑149 (MQ=255)
                         aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                          >  1:811569/1‑149 (MQ=255)
                         aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                          <  2:241024/149‑1 (MQ=255)
                         aGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCtttttt                                                                                                                          <  2:905167/149‑1 (MQ=255)
                          gATCGATAAAGGCATTCTGATAACTTTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTg                                                                                                                         <  2:875078/149‑1 (MQ=255)
                          gATCGAAAAAGGCATTCTTATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCCTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTg                                                                                                                         <  2:66958/149‑1 (MQ=255)
                           ttCGATAAAGGCTTTCTGATATCTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                        <  2:604885/148‑1 (MQ=255)
                           aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                        >  1:310210/1‑149 (MQ=255)
                           aTCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTgg                                                                                                                        >  1:1150556/1‑149 (MQ=255)
                                       aTTCTGATAACTGTCATAAGAGACAGTTTTGACTTCCGGGAGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTAACTTTTTTTGCAATCAAGACgg                                                                                                            >  2:361148/1‑149 (MQ=255)
                                        ttCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                           <  1:441722/149‑1 (MQ=255)
                                        ttCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGc                                                                                                           >  2:335707/1‑149 (MQ=255)
                                           tGATAACTTTGATTAGATACAGTTTTCACTTGAGTGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGTTCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCTCGACGGCTGa                                                                                                        <  1:654871/149‑1 (MQ=255)
                                           tGATAACTGTCATAAGAGACAGTTTTGACTTGCGGTTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCGTATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGa                                                                                                        <  1:524437/149‑1 (MQ=255)
                                            gATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAg                                                                                                       >  2:714650/1‑149 (MQ=255)
                                             aTAACTGTCATAAGAGACAGTTTTCCCTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGt                                                                                                      >  1:998614/1‑149 (MQ=255)
                                                 atggcATAAGAGACAGTTTTCACTTACGGGTGCTGATACTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTc                                                                                                  <  2:863929/145‑1 (MQ=255)
                                                      aTAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCAtagt                                                                                             <  2:941994/149‑1 (MQ=255)
                                                          gagaCAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAggg                                                                                         >  1:929721/1‑149 (MQ=255)
                                                             aCAGTTTTCACTTGCGGGTGCTGAGCCTTAATATTTTTCTGGTGCGTAGTACCGTTTTCCATCCCACTACGTTTGCCTTTCAGATCGGCTAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGt                                                                                      <  1:573463/149‑1 (MQ=255)
                                                                 ttttCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTa                                                                                  >  1:462112/1‑149 (MQ=255)
                                                                  tttCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTaa                                                                                 >  2:312874/1‑149 (MQ=255)
                                                                   ttaaattCCGGGTGCGGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTACATACCAATACGTTTGCCTTTACGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTaaa                                                                                <  1:624548/144‑1 (MQ=255)
                                                                    tCACTTCCGGGTGCTGATCATGAATATATTTCTGGTGCGTAGTACCGCTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAAc                                                                               <  2:986088/149‑1 (MQ=255)
                                                                              gTGCTGATCCTGAATATATTTCTGGGGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGtt                                                                     <  1:440217/149‑1 (MQ=255)
                                                                               tgctgcACCTGAATATATTGATGGCGCGTAGTACCGGATTGCATCCCAATACGTTTGCCTTTCAGATCGGCAAACTTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGCGCGTGGTAAACGAAACCTGttt                                                                    <  2:939902/149‑1 (MQ=255)
                                                                               tGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGttt                                                                    >  1:355801/1‑149 (MQ=255)
                                                                                gCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTg                                                                   <  1:241307/149‑1 (MQ=255)
                                                                                 cTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTgg                                                                  >  1:352861/1‑148 (MQ=255)
                                                                                  tGATCCTGAATATATTTCTGGTGCGTAGTACCCTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGCCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCt                                                                 >  1:590197/1‑149 (MQ=255)
                                                                                   gacccTGAATATAATGTTTGTGCGTCGTCCCGCTTTCCCTCAAAATAATTTCGAATTTAAGATCGTCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                                <  2:1186280/146‑1 (MQ=255)
                                                                                   gATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                                >  1:899415/1‑149 (MQ=255)
                                                                                   gATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTa                                                                <  1:244802/149‑1 (MQ=255)
                                                                                    aTCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTAc                                                               >  1:971159/1‑149 (MQ=255)
                                                                                       cTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCt                                                            <  2:141448/149‑1 (MQ=255)
                                                                                          aaTATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                         <  1:79668/149‑1 (MQ=255)
                                                                                          aaTATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCg                                                         <  2:516539/149‑1 (MQ=255)
                                                                                            tatatTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACCGCTGAGTTTACATAGTAGGGCGTGGTAACCGCTCCCTGTTTGCTACGCTCCggg                                                       >  2:698278/1‑149 (MQ=255)
                                                                                              tatTTCTGGTGCGTAGTACCGTGTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGt                                                     >  2:30712/1‑149 (MQ=255)
                                                                                               atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                                    >  1:464225/1‑149 (MQ=255)
                                                                                               atTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTGCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGCCGGCTGAGTTTTCATAGGAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTg                                                    <  1:587333/149‑1 (MQ=255)
                                                                                                   cTGGGGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGatat                                                <  2:229293/149‑1 (MQ=255)
                                                                                                      gTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTCTCTTTTATGGCAAACACGACGGCTGAGGTTTCATAGTAGGGCGTGGTAAAAGATACCTGTTTGCTACGCTCCGGGGTGATATCCa                                             >  1:585864/1‑149 (MQ=255)
                                                                                                         cGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATAc                                          <  2:629802/149‑1 (MQ=255)
                                                                                                             ttaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                                      <  2:1160366/148‑1 (MQ=255)
                                                                                                             gtaCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGga                                      <  1:335707/149‑1 (MQ=255)
                                                                                                              taCCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGgag                                     >  1:1097606/1‑149 (MQ=255)
                                                                                                                ccGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGAGTGGTAATCTATACCTGTTTGCTACGCTCCGGGGTGATATCCATAAAGGAGAt                                   >  1:79454/1‑149 (MQ=255)
                                                                                                                 cGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTGGGGAATCACGACGGCTGAGTGTTCATAGTAGGGGGTGGTAAGCGATTCCTGTTTGCTTCGCTCAGGGGTGATATCAATACCGGAGAtt                                  >  2:686844/1‑149 (MQ=255)
                                                                                                                      tCCATCCCAATACGTTTGCCTTTCAGATCCGCAAACGTTTTGTAGGTATCTTTTTTGGCTATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGTGGTGATATCCATACCGGAGATTACGGc                             <  2:871005/149‑1 (MQ=255)
                                                                                                                       ccATCCCAATACGTTTGCCTTTCAGATCGGCTAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAATTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGCGGTGATATACATACCGGAGATTACGGCg                            >  1:668016/1‑149 (MQ=255)
                                                                                                                            acAAAACGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCatt                       >  1:600784/2‑148 (MQ=255)
                                                                                                                             cAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATACATACCGGAGATTAAGGCGTCatat                      >  1:1079676/1‑149 (MQ=255)
                                                                                                                                aaCGTTTGCCGATCTGAAAGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATGCATACCGGAGATTACGGCGTCATAtttt                   >  1:1026076/2‑149 (MQ=255)
                                                                                                                                 aCGTTTGCCTTTCAGATCGGCAATCGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTc                  <  1:361148/149‑1 (MQ=255)
                                                                                                                                    tttgaatttCAGATCGGGAAACGTTTTGTAGGTATCTTTTTAGGCAATCACAACGGCTGAGCTTTCAAAGTAGGGCGTCGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGa               <  2:60514/143‑1 (MQ=255)
                                                                                                                                          tttCAGATCGGCAAACGTTTTGTATGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTATGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATACATACCGGAGATTACGGCGTCATATTTTGTGACTTTCa         >  2:688114/1‑149 (MQ=255)
                                                                                                                                           ttCAGTTCGGCATACGTTTTGTAGTTATCTTTTTTGGCAATCACGACGGCTGCGTTTTGATAGTATGGCGGGGTAAACGCTACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAg        <  2:586137/149‑1 (MQ=255)
                                                                                                                                           ttCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAg        >  1:1029376/1‑149 (MQ=255)
                                                                                                                                            tCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAgg       <  2:310210/149‑1 (MQ=255)
                                                                                                                                                 tCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACgg  >  1:972571/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TACCCCATCAATACGACCATTTTTCAGATCGATAAAGGCATTCTGATAACTGTCATAAGAGACAGTTTTCACTTCCGGGTGCTGATCCTGAATATATTTCTGGTGCGTAGTACCGTTTTCCATCCCAATACGTTTGCCTTTCAGATCGGCAAACGTTTTGTAGGTATCTTTTTTGGCAATCACGACGGCTGAGTTTTCATAGTAGGGCGTGGTAAACGATACCTGTTTGCTACGCTCCGGGGTGATATCCATACCGGAGATTACGGCGTCATATTTTCTGAATTTCAGGGACGG  >  NC_000913/900063‑900356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑