Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 14,132 C→T 8.7% intergenic (+53/‑36) dnaK → / → dnaJ chaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,1320CT 8.7% 252.7 / 10.5 92intergenic (+53/‑36)dnaK/dnaJchaperone Hsp70, with co‑chaperone DnaJ/chaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base C (40/43);  new base T (3/5);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

CAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGT  >  NC_000913/13987‑14275
                                                                                                                                                 |                                                                                                                                               
cagctgACTGCCTGTGCTGATGCTTCTGCAAACAACGCGATAGATGACGATGTTGGCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGc                                                                                                                                              <  1:548992/149‑1 (MQ=255)
 agcagACTGCCGGTGCTTATGCTTCAGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAAAAATCGCCCTATAAACGGGTAAATATACTGACACGGGCGGAGAGGAAATTCCCCTTCGcc                                                                                                                                             <  2:1066817/149‑1 (MQ=255)
  ccagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGAGGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGccc                                                                                                                                            <  1:1088900/148‑1 (MQ=255)
   cagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCCTCTCCGCCCg                                                                                                                                           >  2:120117/1‑149 (MQ=255)
    agACTGCCGTTGCCGATGCTTCTGCAAACAACGCGAAAGATGACGATGGCGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTCATTATCCTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGt                                                                                                                                          <  1:1177769/149‑1 (MQ=255)
      aCTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTATTTATACTGACACGGGCGAAGGGGAATTTCATCTCCTCCCGtcc                                                                                                                                        >  1:791268/1‑147 (MQ=255)
        tGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAt                                                                                                                                      >  1:755644/1‑149 (MQ=255)
                cTGATGCTTCTGCAAACAACGCTAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATATCCTCTCCGCCCGTGCATTCATCTAg                                                                                                                              <  1:910689/149‑1 (MQ=255)
                   aTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAgggg                                                                                                                           >  2:1001044/1‑149 (MQ=255)
                       ttCTGCAAACAACGCGAAAGCTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                                       >  1:176426/1‑149 (MQ=255)
                       ttCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAAt                                                                                                                       >  1:1117416/1‑149 (MQ=255)
                           gCAAACAACGCGAATGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAACGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGCGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCTATTTaa                                                                                                                   >  1:781892/1‑149 (MQ=255)
                             aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                                 <  1:1058821/149‑1 (MQ=255)
                             aaaCAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaa                                                                                                                 <  1:609637/149‑1 (MQ=255)
                               acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                               >  1:303851/1‑149 (MQ=255)
                               acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGCCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATTTaaaaaa                                                                                                               >  2:363032/1‑149 (MQ=255)
                                 aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                             >  1:873460/1‑149 (MQ=255)
                                 aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGa                                                                                                             <  2:723094/149‑1 (MQ=255)
                                  acGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAt                                                                                                            <  1:362852/149‑1 (MQ=255)
                                      gAAATAAGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGCCAATAAATATTGGCCCTATAAACGGTTTATTATACTGACAAGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTATAAATGATGGCt                                                                                                        <  1:240475/149‑1 (MQ=255)
                                      gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCt                                                                                                        >  1:942262/1‑149 (MQ=255)
                                      gAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCATCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCt                                                                                                        >  1:307302/1‑149 (MQ=255)
                                        aaGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTaa                                                                                                      <  1:788177/149‑1 (MQ=255)
                                         aGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAg                                                                                                     >  2:778669/1‑149 (MQ=255)
                                          gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTGTAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                                    <  1:372139/149‑1 (MQ=255)
                                          gATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGc                                                                                                    <  1:127057/149‑1 (MQ=255)
                                             gACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAg                                                                                                 >  2:204730/1‑149 (MQ=255)
                                               cGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCTTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGat                                                                                               >  2:603976/1‑149 (MQ=255)
                                                gATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGatt                                                                                              >  2:1065607/1‑149 (MQ=255)
                                                   gttgtCGACGCTGTATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAAGGGGTAATTATAGTGACTCGGGAGTAGGTGAATTTCCTCTCCGCCCGTGCATTCATCTATTGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                                           <  2:526371/149‑1 (MQ=255)
                                                   gttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGattatt                                                                                           <  1:1127813/149‑1 (MQ=255)
                                                    ttgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACTGGTAATTATACTGACCCGGGCGAAGGGGAATTTCCTCTCCGCCGGTGCATTCTTCTAGGGGCAATTTAAAAAAGATGGGTAAGCAAGAttatta                                                                                          <  2:995438/149‑1 (MQ=255)
                                                     tgtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTAc                                                                                         <  1:224451/149‑1 (MQ=255)
                                                      gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTGTCCGCCCGTGCATTAATCTAGGGCAAATTTAAAAAAGATGGCTAAGCATGTTTATTAcc                                                                                        >  2:660934/1‑148 (MQ=255)
                                                      gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACg                                                                                        >  1:21593/1‑149 (MQ=255)
                                                         gACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACgaga                                                                                     >  1:1177044/1‑149 (MQ=255)
                                                          aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAt                                                                                    >  1:261301/1‑149 (MQ=255)
                                                             atcaaTTTCCAGAAGTCAATGAAAAAAAATAATCGCCCTAAAAACGGCTAATTATACTTACAAGTGCGAAGGGGAATTTCCTCTACGCCCGTGCATTCATCTAGTTGAAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGAtttt                                                                                 <  2:768308/146‑1 (MQ=255)
                                                              tGAATTTGAAGAAGTCAAAGCCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTa                                                                                >  2:526896/1‑149 (MQ=255)
                                                                aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                              <  2:596181/149‑1 (MQ=255)
                                                                aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAgg                                                                              <  1:452322/149‑1 (MQ=255)
                                                                  tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                                      >  2:1132508/1‑88 (MQ=25)
                                                                  tttGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGGAGAGGAAATTCCCCTTCGCCCGTgtca                                                                                                                                      <  1:1132508/91‑4 (MQ=25)
                                                                    tGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGtt                                                                          >  2:350661/1‑149 (MQ=255)
                                                                     gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                                         <  1:947493/149‑1 (MQ=255)
                                                                     gaagaaGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                                         >  1:494028/1‑149 (MQ=255)
                                                                     gaagaaGTCAAAGACAAAAAATAATCGCACTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCtttt                                                                         >  2:302551/1‑149 (MQ=255)
                                                                      aagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTc                                                                        <  1:172104/149‑1 (MQ=255)
                                                                         aagTCAAACACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaa                                                                     <  2:568609/149‑1 (MQ=255)
                                                                           gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                                   <  1:290868/149‑1 (MQ=255)
                                                                           gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaaa                                                                   >  1:75820/1‑149 (MQ=255)
                                                                             cAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                                 >  1:389424/1‑149 (MQ=255)
                                                                             cAAAGACAAAAAATAAGCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGAGGGCTACGCCAGATTATTACGAGATTTTAGGCGTTTCCAAAACa                                                                 <  1:206645/149‑1 (MQ=255)
                                                                              aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAg                                                                <  2:656595/149‑1 (MQ=255)
                                                                              aaaGACAAAAAATAATCGCCCTATAAACGGGTAATTATACGGACACCGGCGAAGGGGAATTTCCTCTCCGCCAGTGCATACTTCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACTAGAGTTTAGGATTTTCCAAAACat                                                                >  1:683795/1‑148 (MQ=255)
                                                                                aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCg                                                              <  1:1109858/149‑1 (MQ=255)
                                                                                 gACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCgg                                                             <  1:1001044/149‑1 (MQ=255)
                                                                                  aCAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGTCGTTTCCACAACAGCGGa                                                            >  1:178601/1‑149 (MQ=255)
                                                                                   cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTATCCAAAACAGCGGaa                                                           >  1:839428/1‑149 (MQ=255)
                                                                                     aaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAaga                                                         <  2:923239/149‑1 (MQ=255)
                                                                                      aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                                        >  2:418532/1‑148 (MQ=255)
                                                                                      aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAGTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagag                                                        >  2:889523/1‑149 (MQ=255)
                                                                                      aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGAATATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAagac                                                        >  1:1172851/1‑148 (MQ=255)
                                                                                         ataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGt                                                     <  1:500939/149‑1 (MQ=255)
                                                                                            atCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGaa                                                  <  1:204730/149‑1 (MQ=255)
                                                                                              cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAAt                                                <  1:363653/149‑1 (MQ=255)
                                                                                               tcccTATAAACGGGTAATTATATTGACACGGGCGAAGGTGAATTTCCTCTCAGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATc                                               <  2:247476/148‑1 (MQ=255)
                                                                                                   tataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCTAGATTATTACTAGATTTTAGGAGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaa                                           >  1:59239/1‑149 (MQ=255)
                                                                                                    ataAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGaaa                                          >  1:1177249/1‑149 (MQ=255)
                                                                                                        aCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTTTTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGTcc                                      >  1:1117218/1‑149 (MQ=255)
                                                                                                         cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCt                                     <  1:724384/149‑1 (MQ=255)
                                                                                                          gggTAATTATACTGACACGGGCGAAGGGGAATTTCGTCTCCGCCCGTGCATTCATCTAGGGGCAATATAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    <  2:1163392/149‑1 (MQ=255)
                                                                                                          gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    >  1:961842/1‑149 (MQ=255)
                                                                                                          gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                    <  1:937262/149‑1 (MQ=255)
                                                                                                           ggTAACTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTAc                                   <  1:550521/149‑1 (MQ=255)
                                                                                                            gTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACa                                  >  1:38847/1‑149 (MQ=255)
                                                                                                                 tataCTGACACTGGCCTAGGGGAATTTCCTCTCCGCGCGTGCATTCATCTAGGCGCACTTTAAAAAAGCTGGCTTAGCAAGATTTTTACGAGATTTTCGGCGTTTCCAACACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                             <  2:898136/149‑1 (MQ=255)
                                                                                                                 tataCTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGc                             <  1:412355/149‑1 (MQ=255)
                                                                                                                          acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg                    >  2:503152/1‑149 (MQ=255)
                                                                                                                          acGGGCGGAGAGGAAATTCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATg                    >  2:507573/1‑149 (MQ=255)
                                                                                                                           cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGa                   <  2:746640/149‑1 (MQ=255)
                                                                                                                            gggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGaa                  <  2:791268/149‑1 (MQ=255)
                                                                                                                              gCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAAt                >  2:447900/1‑149 (MQ=255)
                                                                                                                               cGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATa               <  1:611222/149‑1 (MQ=255)
                                                                                                                                gAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATac              <  1:350661/149‑1 (MQ=255)
                                                                                                                                     gtaaTTTCCTCACAGCCAGGTCATTCATCTAGGGGCAATTTAAAAAACATGGCTAAGCAAGATTATTAGGATATCTTAGGCGTTTGCAAAACAGCGTAAGAGCGTGAAATCAGAATGGCCTTCAAACGCCTGGCCATGAAATAACAccc         <  2:188329/147‑1 (MQ=255)
                                                                                                                                      gAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCg        >  1:1053400/1‑149 (MQ=255)
                                                                                                                                        aattCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  1:503152/147‑1 (MQ=255)
                                                                                                                                        aattCCCCTTCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  1:507573/147‑1 (MQ=255)
                                                                                                                                        aTTTCCTCTCCGCCCGTGCATTAATCTAGGTGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGa      <  2:839428/149‑1 (MQ=255)
                                                                                                                                          ttCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGAcc    <  1:140525/149‑1 (MQ=255)
                                                                                                                                            ccTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGt  >  1:467224/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGT  >  NC_000913/13987‑14275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: