Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,145,940 A→C 8.2% intergenic (+41/+71) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,9400AC 8.2% 171.6 / 4.3 70intergenic (+41/+71)rluC/yceF23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base C (2/4);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTT  >  NC_000913/1145793‑1146088
                                                                                                                                                   |                                                                                                                                                    
cAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAg                                                                                                                                                     <  1:691639/149‑1 (MQ=255)
   cGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCg                                                                                                                                                  >  2:709020/1‑149 (MQ=255)
    gTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTTTGGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGt                                                                                                                                                 <  1:1121181/149‑1 (MQ=255)
     ttGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGtt                                                                                                                                                >  1:1056178/1‑149 (MQ=255)
        aaGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCGTGATAAGGCGTTTAc                                                                                                                                             <  2:547574/149‑1 (MQ=255)
          ggatACTCATGCTGGGTCCGCTGTCGTGATGCGTATCGAATGTCCGATGGCTGAAGGTTTGAAGCGTTGTATGCAAAAGCTGCTTAACGCGCGCTAATACAACCATAAAAACCTGATAATTGGCGGTTGGCCTGATAAGGCGTTTAcgc                                                                                                                                           <  2:1112199/146‑1 (MQ=255)
             tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAAcgcctt                                                                                                                                        <  1:620401/149‑3 (MQ=255)
             tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTAcgccgc                                                                                                                                        <  2:372105/149‑1 (MQ=255)
                 cATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTCGGCCTGATGCGGCGTAAACGCCTTATCa                                                                                                                                    <  2:389818/149‑1 (MQ=255)
                  aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                   <  2:756271/149‑1 (MQ=255)
                  aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                   >  1:601410/1‑149 (MQ=255)
                        ggACCGGTGCGGTGATGGGTATCGAATCTCCGATGGATGTATGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTTTTTTTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGgccgcc                                                                                                                             <  2:291893/149‑1 (MQ=255)
                         gACCGGTGAGGTGATGCGTATCGAAGCTCCGATGTATGAACGTTTGAAGCGTTCTTTGCAAAAGCTGCGAAACGCGCGCTAATACAAGCATATACACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                            <  1:935040/149‑1 (MQ=255)
                         ccccGGTGAGGTGATGCGTAGCGAAGCTCCGATGGATGCAGGTTTGAAGCGTTGTTTGCAAAAGCGGCGTACCGCGCGCTAATCCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGTTAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                            <  1:1017861/147‑1 (MQ=255)
                            cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTCACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                         <  1:100373/149‑1 (MQ=255)
                            cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAACCCAGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                         >  1:900545/1‑149 (MQ=255)
                            cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAACGCCTTATCAGgccaaccgcc                                                                                                                         <  1:497779/149‑8 (MQ=38)
                                  ggtgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAtt                                                                                                                   <  1:336500/149‑1 (MQ=255)
                                    tgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGc                                                                                                                 >  1:722188/1‑149 (MQ=255)
                                             tCGAAGCTCCGATGGATGAAGGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATACGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGa                                                                                                        <  1:698117/149‑1 (MQ=255)
                                                aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                     >  2:649758/1‑149 (MQ=255)
                                                aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                     >  2:322216/1‑149 (MQ=255)
                                                aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                     >  2:317731/1‑149 (MQ=255)
                                                       gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                              >  2:65105/1‑149 (MQ=255)
                                                       gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGGTGGCCTGATAAGGCGTGTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                              <  2:776698/149‑1 (MQ=255)
                                                            atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                         <  1:180424/149‑1 (MQ=255)
                                                            atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                         >  2:844742/1‑149 (MQ=255)
                                                            atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                         >  1:849980/1‑149 (MQ=255)
                                                             tgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGgc                                                                                        >  1:857610/1‑149 (MQ=255)
                                                                     tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCATGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                <  1:791187/149‑1 (MQ=255)
                                                                     tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                <  1:709020/149‑1 (MQ=255)
                                                                        agccctctttGCAAAAGCGGCGTCACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCTGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                             <  1:852425/142‑1 (MQ=255)
                                                                        aGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                             <  1:158548/149‑1 (MQ=255)
                                                                                  gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                   >  2:533708/1‑149 (MQ=255)
                                                                                  gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                   >  1:654204/1‑149 (MQ=255)
                                                                                   cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                  >  1:786271/1‑149 (MQ=255)
                                                                                   cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                  <  2:866959/149‑1 (MQ=255)
                                                                                    aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAACCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAACCTATCCCATCAGCgg                                                                 >  1:998541/1‑149 (MQ=255)
                                                                                    aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTCTCCCATCAGCgg                                                                 >  1:1161525/1‑149 (MQ=255)
                                                                                    aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCgg                                                                 >  2:349321/1‑149 (MQ=255)
                                                                                     aaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGAATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCggg                                                                <  2:930043/149‑1 (MQ=255)
                                                                                           gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttc                                                          <  2:801661/149‑1 (MQ=255)
                                                                                               aaCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCACATCCGGCGCAAAAAACTAACCCATCAGCGGGTTTttccctt                                                      >  2:124407/1‑149 (MQ=255)
                                                                                                  gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCTCATCAGTCCGCCAGCACCGATTGCAGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttcccttccc                                                   >  2:212393/1‑149 (MQ=255)
                                                                                                    gcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGa                                                 >  2:827739/1‑149 (MQ=255)
                                                                                                      gcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGATTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATACCATCAGCGGGTTTTTCCCTTCCCGACg                                               >  2:124145/1‑149 (MQ=255)
                                                                                                       cTAATACAAGTATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                              <  1:626993/149‑1 (MQ=255)
                                                                                                        tAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                             >  1:359150/1‑149 (MQ=255)
                                                                                                          aTACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACTTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc                                           <  2:815374/149‑1 (MQ=255)
                                                                                                            aCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCTTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATGCCCTGAGCGGGTTTTTCGCTTCCAGACGTTACAt                                         >  2:664653/1‑149 (MQ=255)
                                                                                                              aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACctct                                       >  1:846167/1‑149 (MQ=255)
                                                                                                              aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACATCt                                       >  1:128184/1‑149 (MQ=255)
                                                                                                                gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCGCATCAGCGGGTTTTTCCCTTCCCGACGTACCAGCTgc                                     >  2:781533/1‑148 (MQ=255)
                                                                                                                gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTCACATCTGa                                     >  2:1108008/1‑149 (MQ=255)
                                                                                                                gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                     <  1:953617/149‑1 (MQ=255)
                                                                                                                gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                     <  1:528829/149‑1 (MQ=255)
                                                                                                                gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                     <  1:977435/149‑1 (MQ=255)
                                                                                                                     taAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAg                                <  1:196706/149‑1 (MQ=255)
                                                                                                                      aaaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATAAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgc                               >  1:703415/1‑149 (MQ=255)
                                                                                                                       aaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAgcg                              >  1:1169196/1‑149 (MQ=255)
                                                                                                                         ccTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg                            >  1:202956/1‑149 (MQ=255)
                                                                                                                             aTAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTa                        <  1:549996/149‑1 (MQ=255)
                                                                                                                              tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAg                       <  1:315759/149‑1 (MQ=255)
                                                                                                                               aaTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGc                      <  1:649758/149‑1 (MQ=255)
                                                                                                                                aTGGGCGGTTGGCATGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg                     <  2:857610/149‑1 (MQ=255)
                                                                                                                                   ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTa                  <  1:65105/149‑1 (MQ=255)
                                                                                                                                     cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTaaa                <  2:1056178/149‑1 (MQ=255)
                                                                                                                                          ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACTGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac           >  2:619617/1‑149 (MQ=255)
                                                                                                                                          ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGGGAGTAGCGGTAacccaac           >  2:858071/1‑149 (MQ=255)
                                                                                                                                              tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGAAGCGCCGTAACCACATCCGGCGCAAAAACCTATCCCATCCGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTACACCACCCCGc       >  1:723643/1‑149 (MQ=255)
                                                                                                                                                   aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGtt  >  1:888531/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGCGTTGAAGTTTACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTT  >  NC_000913/1145793‑1146088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: