Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,145,954 C→T 5.7% intergenic (+55/+57) rluC → / ← yceF 23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,145,9540CT 5.7% 196.0 / 2.1 70intergenic (+55/+57)rluC/yceF23S rRNA pseudouridine(955,2504,2580) synthase/m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (36/30);  new base T (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCT  >  NC_000913/1145806‑1146102
                                                                                                                                                    |                                                                                                                                                    
tACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTAcgccgc                                                                                                                                                      <  2:372105/149‑1 (MQ=255)
    cATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTCGGCCTGATGCGGCGTAAACGCCTTATCa                                                                                                                                                  <  2:389818/149‑1 (MQ=255)
     aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                                 >  1:601410/1‑149 (MQ=255)
     aTCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAg                                                                                                                                                 <  2:756271/149‑1 (MQ=255)
           ggACCGGTGCGGTGATGGGTATCGAATCTCCGATGGATGTATGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTTTTTTTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATAAGgccgcc                                                                                                                                           <  2:291893/149‑1 (MQ=255)
            gACCGGTGAGGTGATGCGTATCGAAGCTCCGATGTATGAACGTTTGAAGCGTTCTTTGCAAAAGCTGCGAAACGCGCGCTAATACAAGCATATACACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                          <  1:935040/149‑1 (MQ=255)
            ccccGGTGAGGTGATGCGTAGCGAAGCTCCGATGGATGCAGGTTTGAAGCGTTGTTTGCAAAAGCGGCGTACCGCGCGCTAATCCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGTTAAGGCGTTTACGCCGCATCAGGCCGCCa                                                                                                                                          <  1:1017861/147‑1 (MQ=255)
               cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTCACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                                       <  1:100373/149‑1 (MQ=255)
               cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAACCCAGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCa                                                                                                                                       >  1:900545/1‑149 (MQ=255)
               cGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATGCGGCGTAAACGCCTTATCAGgccaaccgcc                                                                                                                                       <  1:497779/149‑8 (MQ=38)
                     ggtgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAtt                                                                                                                                 <  1:336500/149‑1 (MQ=255)
                       tgaTGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGc                                                                                                                               >  1:722188/1‑149 (MQ=255)
                                tCGAAGCTCCGATGGATGAAGGGTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATACTGGGCGGTTGGCCTGATACGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGa                                                                                                                      <  1:698117/149‑1 (MQ=255)
                                   aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                                   >  2:317731/1‑149 (MQ=255)
                                   aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                                   >  2:322216/1‑149 (MQ=255)
                                   aaGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGt                                                                                                                   >  2:649758/1‑149 (MQ=255)
                                          gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                            >  2:65105/1‑149 (MQ=255)
                                          gatggatgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGGTGGCCTGATAAGGCGTGTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACcaca                                                                                                            <  2:776698/149‑1 (MQ=255)
                                               atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                                       >  2:844742/1‑149 (MQ=255)
                                               atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                                       >  1:849980/1‑149 (MQ=255)
                                               atgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCgg                                                                                                       <  1:180424/149‑1 (MQ=255)
                                                tgAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGgc                                                                                                      >  1:857610/1‑149 (MQ=255)
                                                        tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCATGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                              <  1:791187/149‑1 (MQ=255)
                                                        tGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCaaaaaa                                                                                              <  1:709020/149‑1 (MQ=255)
                                                           agccctctttGCAAAAGCGGCGTCACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCTGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                           <  1:852425/142‑1 (MQ=255)
                                                           aGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTa                                                                                           <  1:158548/149‑1 (MQ=255)
                                                                     gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                                 >  2:533708/1‑149 (MQ=255)
                                                                     gCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGc                                                                                 >  1:654204/1‑149 (MQ=255)
                                                                      cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                                >  1:786271/1‑149 (MQ=255)
                                                                      cAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCg                                                                                <  2:866959/149‑1 (MQ=255)
                                                                       aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAACCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAACCTATCCCATCAGCgg                                                                               >  1:998541/1‑149 (MQ=255)
                                                                       aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTCTCCCATCAGCgg                                                                               >  1:1161525/1‑149 (MQ=255)
                                                                       aaaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCgg                                                                               >  2:349321/1‑149 (MQ=255)
                                                                        aaaGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGAATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCggg                                                                              <  2:930043/149‑1 (MQ=255)
                                                                              gCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttc                                                                        <  2:801661/149‑1 (MQ=255)
                                                                                  aaCGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCACATCCGGCGCAAAAAACTAACCCATCAGCGGGTTTttccctt                                                                    >  2:124407/1‑149 (MQ=255)
                                                                                     gcgcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCTCATCAGTCCGCCAGCACCGATTGCAGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTttcccttccc                                                                 >  2:212393/1‑149 (MQ=255)
                                                                                       gcgcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGa                                                               >  2:827739/1‑149 (MQ=255)
                                                                                         gcTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGATTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATACCATCAGCGGGTTTTTCCCTTCCCGACg                                                             >  2:124145/1‑149 (MQ=255)
                                                                                          cTAATACAAGTATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGt                                                            <  1:626993/149‑1 (MQ=255)
                                                                                           tAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTa                                                           >  1:359150/1‑149 (MQ=255)
                                                                                             aTACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACTTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAAc                                                         <  2:815374/149‑1 (MQ=255)
                                                                                               aCAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCTTCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATGCCCTGAGCGGGTTTTTCGCTTCCAGACGTTACAt                                                       >  2:664653/1‑149 (MQ=255)
                                                                                                 aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACctct                                                     >  1:846167/1‑149 (MQ=255)
                                                                                                 aaGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACATCt                                                     >  1:128184/1‑149 (MQ=255)
                                                                                                   gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCGCATCAGCGGGTTTTTCCCTTCCCGACGTACCAGCTgc                                                   >  2:781533/1‑148 (MQ=255)
                                                                                                   gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTCACATCTGa                                                   >  2:1108008/1‑149 (MQ=255)
                                                                                                   gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                                   <  1:953617/149‑1 (MQ=255)
                                                                                                   gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                                   <  1:977435/149‑1 (MQ=255)
                                                                                                   gCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGa                                                   <  1:528829/149‑1 (MQ=255)
                                                                                                        taAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAg                                              <  1:196706/149‑1 (MQ=255)
                                                                                                         aaaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATAAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAgc                                             >  1:703415/1‑149 (MQ=255)
                                                                                                          aaCCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAgcg                                            >  1:1169196/1‑149 (MQ=255)
                                                                                                            ccTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGcgcg                                          >  1:202956/1‑149 (MQ=255)
                                                                                                                aTAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTa                                      <  1:549996/149‑1 (MQ=255)
                                                                                                                 tAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAg                                     <  1:315759/149‑1 (MQ=255)
                                                                                                                  aaTGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGc                                    <  1:649758/149‑1 (MQ=255)
                                                                                                                   aTGGGCGGTTGGCATGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCg                                   <  2:857610/149‑1 (MQ=255)
                                                                                                                      ggCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTa                                <  1:65105/149‑1 (MQ=255)
                                                                                                                        cGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTaaa                              <  2:1056178/149‑1 (MQ=255)
                                                                                                                             ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACTGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAaaccaac                         >  2:619617/1‑149 (MQ=255)
                                                                                                                             ggCCTGATGCGGCGTAAACGCCTTATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACCTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGGGAGTAGCGGTAacccaac                         >  2:858071/1‑149 (MQ=255)
                                                                                                                                 tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGAAGCGCCGTAACCACATCCGGCGCAAAAACCTATCCCATCCGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTACACCACCCCGc                     >  1:723643/1‑149 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGtt                >  1:888531/1‑149 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGTGAAAAAAACTATCCCATCAGCGGGTTTTTCCCGTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTa               >  2:866274/1‑149 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGGCACCACATCCGGCGCAAAAAAGTAGCCGATCAGCGGGTTTTTCGATTCCCGACGTAACATCTGACACAGCGCGATAAGCGGTATACCAACCAGAGTGATa               >  2:711042/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTACCCCCAGCCGGCGCAAAAAACTATCCCAGAAGCGGGTTTTTACCTTCCCGACGTAACATAGTCCCCAGAGCGATTAGCGGTAAACCAACCAGCGTGTTat              >  2:643882/1‑148 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGCCACAGCGCGATTAGCGGTAAACCAACCCGCGTGTTAg              >  1:153423/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAg              <  2:151241/149‑1 (MQ=255)
                                                                                                                                          gTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAACCTATCCCATCAGCGGGTTTTTCCCTTCCCGACGAAACAGCTGACACCGCGCGATGAGCGGTAAACCAACCAGCGTGGTAGGa            >  1:1124037/1‑149 (MQ=255)
                                                                                                                                           tttACGCCGCATCTGGCGGGCAGCACCGATTGGCGGATGCGACGTATCCACATCCGGCGCAAAAAACTATCCCATGAGCGGGTTTTTCCCTTGCCGTCGTACCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGAt           <  2:544538/149‑1 (MQ=255)
                                                                                                                                                gccgcATCAGGCCGCCAGCCCCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAGCCAGCGTGTTAGGATCCCGc      >  2:833722/1‑149 (MQ=255)
                                                                                                                                                gccgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGCCGTAACATCTGACACAGCGCGCTTAGCGGTAAACCAACCAGCGTGTTAGGATCACGc      >  1:266839/1‑149 (MQ=255)
                                                                                                                                                  cgcATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGccc    <  2:722188/149‑1 (MQ=255)
                                                                                                                                                    cATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCACCCCGCGTGTTAGGATCACGCCCct  >  2:977152/1‑149 (MQ=255)
                                                                                                                                                    cATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCct  <  1:827739/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACTCATCCGGGGACCGGTGAGGTGATGCGTATCGAAGCTCCGATGGATGAAGGTTTGAAGCGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAATACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCT  >  NC_000913/1145806‑1146102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: