Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,684 A→T 7.1% intergenic (+26/+34) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6840AT 7.1% 186.8 / 3.3 69intergenic (+26/+34)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (29/35);  new base T (2/3);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAT  >  NC_000913/1314536‑1314828
                                                                                                                                                    |                                                                                                                                                
aTGTCAGTGTGGTACATGGATATCGATACAACCGCCAATTATAAGCTGGGCGGTGCCCAGCCACACGATCGCGTACGACTCTATCCGTGGGTGTTTAGGGTCTCAGCAGGAGATCGTTTTTAATTCCGCACACAAACGACCCCGTAata                                                                                                                                                  >  1:880702/1‑149 (MQ=255)
     aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                                                                             <  1:36683/149‑1 (MQ=255)
        gtgGTACATGGATATCGATACCACCGCCAATTATAAGCTTGGCGTTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                                                                          <  1:613301/149‑1 (MQ=255)
         tgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTc                                                                                                                                         <  1:347267/149‑1 (MQ=255)
          ggTACATGGATTTCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                                                                        <  2:1170936/149‑2 (MQ=255)
              cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                    <  1:441505/149‑1 (MQ=255)
              aaTGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACTCCTCTATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCTCACAAAAACGACCCCGTATATTACGGGGTcgtttt                                                                                                                                    <  2:822662/148‑6 (MQ=255)
               aTGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTGTTTTCTCAGCAGCATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgttttt                                                                                                                                   <  1:402832/149‑7 (MQ=255)
                tGGATATCGATACCACAGCCAATTATAATCTTGGCCGTGCACAGCAACACGATAGCTTACGCCTCGTTCCGTGGGTGTTTATGTTCTCAGCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                                                                  <  2:96026/149‑1 (MQ=255)
                tGGAAATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                                                                  <  1:750713/149‑1 (MQ=255)
                 ggATATCGATACCACCGCCAATTATAATCTGGGCGGTGCACAGCAACACGATAGCGTCCGCCTCGATCCGTGGGTGTTTATGGTGGCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAGTATACGGGGTCATTATggc                                                                                                                                 >  1:766518/1‑148 (MQ=255)
                     atCGATACAACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                                                                                             >  2:374892/1‑149 (MQ=255)
                      tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                            <  1:816156/149‑1 (MQ=255)
                        gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACCCGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATAGTTTTTAATTCCGCACAAAAACGACACCGTAATATACGCGGTCAATAAGGACCTGGta                                                                                                                          >  2:238899/1‑149 (MQ=255)
                         aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAGATACGGGGTCAATAAGGACATGGtat                                                                                                                         >  2:168013/1‑149 (MQ=255)
                            ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                                                                      >  1:1163029/1‑149 (MQ=255)
                            ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAAATCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                                                                      <  1:841211/149‑1 (MQ=255)
                              accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAgg                                                                                                                    >  2:1045539/1‑149 (MQ=255)
                                        ttaatgtGGGCGGTGCACAGATACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCGCAGCAGGATATCGTTTTTAAATCCTCACAAAAACGAACCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  2:100867/143‑1 (MQ=255)
                                        ataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  1:351683/149‑1 (MQ=255)
                                        ataAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                                                                          <  2:395728/149‑1 (MQ=255)
                                           aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttc                                                                                                       <  1:85479/149‑1 (MQ=255)
                                            gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGGCAATAAGGACATGGTATAAAGGGTATTATTTCTTCg                                                                                                      >  2:485614/1‑149 (MQ=255)
                                             cTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                     >  2:504215/1‑149 (MQ=255)
                                                          aGCAACACGATAGCGTACGCCTCGATCCGGGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                        <  2:128760/149‑1 (MQ=255)
                                                            cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGt                                                                                      <  2:20442/149‑1 (MQ=255)
                                                               cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                                                                   <  1:236182/149‑1 (MQ=255)
                                                                  gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCCTCAGTTTCaga                                                                                >  2:1091722/1‑149 (MQ=255)
                                                                  gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                <  2:1161385/149‑1 (MQ=255)
                                                                       cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGaa                                                                           <  2:862341/149‑1 (MQ=255)
                                                                       cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGaa                                                                           <  1:870458/149‑1 (MQ=255)
                                                                        gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAt                                                                          >  1:345590/1‑149 (MQ=255)
                                                                           cGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTATGCCATCAGTTTCAGAGCGAATaaa                                                                       >  2:356379/1‑149 (MQ=255)
                                                                            gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                                                                      <  1:1045539/149‑1 (MQ=255)
                                                                              cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAAGGTATAAAGGGTATTATATCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaaa                                                                    >  1:531550/1‑149 (MQ=255)
                                                                               tCGATCCTTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGTTCAATAAGGACATGGTTTAAAGGGTATTATTTCTTAGCTTCTACGCCAACAGTTTCAGAGCGAATTAAAAAt                                                                   >  2:700503/1‑149 (MQ=255)
                                                                               tCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                   >  2:244998/1‑149 (MQ=255)
                                                                                 gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATATGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAttt                                                                 >  1:753426/1‑149 (MQ=255)
                                                                                 gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAttt                                                                 <  2:343926/149‑1 (MQ=255)
                                                                                   tCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                                                               <  2:11601/149‑1 (MQ=255)
                                                                                      gTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAg                                                            <  1:510253/149‑1 (MQ=255)
                                                                                          gtgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAGAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt                                                        >  2:638386/1‑149 (MQ=255)
                                                                                                aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                  <  2:422479/149‑1 (MQ=255)
                                                                                                aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                  >  2:881671/1‑149 (MQ=255)
                                                                                                   ttCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                               >  2:404129/1‑149 (MQ=255)
                                                                                                   ttCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                               >  1:834060/1‑149 (MQ=255)
                                                                                                    tctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAata                                              >  2:397660/1‑149 (MQ=255)
                                                                                                      tcAGGAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                            <  2:330557/149‑1 (MQ=255)
                                                                                                       cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATATGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTc                                           <  1:587230/149‑1 (MQ=255)
                                                                                                          cagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                        <  2:980740/149‑1 (MQ=255)
                                                                                                          cagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGa                                        >  2:22109/1‑149 (MQ=255)
                                                                                                                 tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                 >  1:907609/1‑149 (MQ=255)
                                                                                                                            tCCGCACAAACCGACCCCGTAATATACGTGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAttt                     <  1:1068667/149‑1 (MQ=255)
                                                                                                                              cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTg                    <  1:238899/149‑1 (MQ=255)
                                                                                                                                cacaAAAACGTCCGCGTAATATACGGGGTCAATAATGACATGGTATAAAGTGTATTATTTCTTCGCTTCTACGCCATCAGTTTGAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                  <  2:802339/149‑1 (MQ=255)
                                                                                                                                 acaAAAACGACCCCGTAATATAAGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGACATCAGTTTCAGAGAGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGACAGTCGGCCATTTGCtt                 >  1:859949/1‑149 (MQ=255)
                                                                                                                                   aaaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt               >  2:160229/1‑149 (MQ=255)
                                                                                                                                    aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:873039/149‑1 (MQ=255)
                                                                                                                                    aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:296426/149‑1 (MQ=255)
                                                                                                                                    aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTATCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc              <  1:413326/149‑1 (MQ=255)
                                                                                                                                     tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc             >  2:1081364/5‑149 (MQ=255)
                                                                                                                                     aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc             >  2:554825/1‑149 (MQ=255)
                                                                                                                                      aaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt            <  2:345590/149‑1 (MQ=255)
                                                                                                                                         gACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt         >  1:808388/1‑149 (MQ=255)
                                                                                                                                          aCCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCGTGAtt        >  1:753322/1‑149 (MQ=255)
                                                                                                                                              cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    <  1:361425/149‑1 (MQ=255)
                                                                                                                                              cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    >  1:29674/1‑149 (MQ=255)
                                                                                                                                              cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa    >  2:376494/1‑149 (MQ=255)
                                                                                                                                               gTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTAAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTACTAATTTaaaa   >  1:1083865/1‑149 (MQ=255)
                                                                                                                                                tAATATACGGGGTCAATAAGGACATCGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAt  <  2:1166062/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAT  >  NC_000913/1314536‑1314828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: