Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,409,145 T→C 6.2% noncoding (17/122 nt) fnrS → FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,409,1450TC 6.2% 169.3 / 2.9 66noncoding (17/122 nt)fnrSFNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base C (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

TTGCTTTATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAAT  >  NC_000913/1408999‑1409293
                                                                                                                                                  |                                                                                                                                                    
tttcTTTATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGAAATTTTTCCGGCTGTCTTTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAAAGTct                                                                                                                                                    <  2:579195/146‑2 (MQ=255)
ttGCTTTATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCa                                                                                                                                                    >  1:388738/1‑149 (MQ=255)
  gCTTTATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAg                                                                                                                                                  >  2:249613/1‑149 (MQ=255)
    tttATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCg                                                                                                                                                >  1:968258/1‑149 (MQ=255)
      tATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGAt                                                                                                                                              <  1:761350/149‑1 (MQ=255)
      tATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGAt                                                                                                                                              <  1:981341/149‑1 (MQ=255)
           ttGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTATCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCg                                                                                                                                         >  1:247193/1‑149 (MQ=255)
                   tagtaAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTc                                                                                                                                 >  2:976002/1‑149 (MQ=255)
                     gtaAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCTTTGCGCTcaa                                                                                                                               >  2:664382/1‑147 (MQ=255)
                       aaaTGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGGAAAGTTTGAGCGAAGTCAATAAAGTCTCTACCCATTCAGGGCAATATGTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGAGGAGCGCCata                                                                                                                             >  2:526448/1‑149 (MQ=255)
                        aaTGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAACCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCatat                                                                                                                            >  1:592002/1‑149 (MQ=255)
                            gTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTc                                                                                                                        <  2:259818/149‑1 (MQ=255)
                                   cAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTAGAGCGATGTCAATTAACTCTCTACCCAGTGACCGCCATAGCTCTCTAGCTGGTGAATGGAACGTCAAGCGATGGCCGTGGCGCTCCAGATTGTcttccttc                                                                                                                 >  2:921364/1‑149 (MQ=255)
                                     aaaGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGGGTGAGCGAAGTCAATAAACTCTCTACCCAATCAGGGCAATATCTCTCTTGAAGGTGAATGCAACGTCAAGCGATGGGCGTTGAGCTCCATAACGTATTACTTcat                                                                                                               >  1:680958/1‑147 (MQ=255)
                                      aaGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtt                                                                                                              >  2:554644/1‑149 (MQ=255)
                                         cAATTTTTCCGGCCGTCTGTATACAAACACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCttttt                                                                                                           <  2:372191/149‑1 (MQ=255)
                                          aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGATTGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                          <  1:664382/149‑1 (MQ=255)
                                          aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                          <  2:981970/149‑1 (MQ=255)
                                          aaTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCtttttt                                                                                                          <  2:500122/149‑1 (MQ=255)
                                           aTTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCACTATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGGCTTACTTCCTTTTTTg                                                                                                         <  2:700954/149‑1 (MQ=255)
                                           aTTTTTCCGGCTGTCTGAATACAAAAACGCAGCAAAGTTTGAGCGAATACAATAAACTCTCTACCCATTCAGGGAAATATATCTCTTGCAGCTGAATGCAACGTCCAGCAATGGTCGTTGCTCTACATATTTTATTACTTACttatttt                                                                                                         >  2:415322/1‑148 (MQ=255)
                                             ttttCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGaa                                                                                                       <  1:387419/149‑1 (MQ=255)
                                               ttCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAAtt                                                                                                     <  2:143156/149‑1 (MQ=255)
                                                  cGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACt                                                                                                  >  1:586997/1‑149 (MQ=255)
                                                  cGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACt                                                                                                  <  2:388738/149‑1 (MQ=255)
                                                       gtcttgataCAAAAACGCCGCACAGTTTGAGGGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCTTa                                                                                             <  2:844766/143‑1 (MQ=255)
                                                           gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                    <  2:173664/106‑2 (MQ=255)
                                                           gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                    <  2:173661/106‑2 (MQ=255)
                                                           gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                    >  1:173661/1‑105 (MQ=255)
                                                           gtATACAAAAACGCCGCAAAGTTTGAGCGAAGTAAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGAGAATGCAACGCCCATCGCTTGACGTTgca                                                                                                                                    >  1:173664/1‑105 (MQ=255)
                                                             ataCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACaa                                                                                       >  1:597714/1‑149 (MQ=255)
                                                             ataCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGACAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTACTTTTTTGAATTACTGCATAGCACaa                                                                                       >  1:993226/1‑149 (MQ=255)
                                                               aCAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAAtt                                                                                     <  1:1047293/149‑1 (MQ=255)
                                                                 aaaaaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGa                                                                                   >  1:412277/1‑149 (MQ=255)
                                                                  aaaaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGAt                                                                                  <  1:254832/149‑1 (MQ=255)
                                                                    aaCGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTc                                                                                >  2:1121967/1‑149 (MQ=255)
                                                                       gccgcAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTa                                                                             <  1:976002/149‑1 (MQ=255)
                                                                        ccgaAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTTCATGTGAATGCAACGTCAATCGATTGGCGTTGCGATCCATATTGTCTTACTTCCTTTTTTGAATTACTTCATCGCACAATTGATTCGTac                                                                            <  2:135053/149‑1 (MQ=255)
                                                                          gcAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTacga                                                                          <  2:730526/149‑1 (MQ=255)
                                                                           cAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTacgac                                                                         >  1:995708/1‑149 (MQ=255)
                                                                                tttGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCgc                                                                    >  1:816482/1‑148 (MQ=255)
                                                                                      cGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTtga                                                              <  2:968258/149‑1 (MQ=255)
                                                                                       gAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCCTCGCACAATTGATTCGTACGACGCCGACTTtgat                                                             <  2:336505/149‑1 (MQ=255)
                                                                                          gTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAg                                                          >  1:776794/1‑149 (MQ=255)
                                                                                           tCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGt                                                         >  1:611767/1‑149 (MQ=255)
                                                                                             aaTAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCg                                                       >  1:297431/1‑149 (MQ=255)
                                                                                                 aaCTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATTTTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGAGGAGTCGGCtt                                                   >  2:185588/1‑149 (MQ=255)
                                                                                                     ctgtACCCATTCAGGGAAATATCTCTCTTGGAGGTTAATGCAACGTGAAGCGATGGGCGTTGCGCTTGATATTGTCTTACTTCCTTTTTTGAATTTCTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttt                                               <  2:58940/146‑1 (MQ=255)
                                                                                                     ctctACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttt                                               <  1:368916/149‑1 (MQ=255)
                                                                                                      tctACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCttttttt                                              <  1:554644/149‑1 (MQ=255)
                                                                                                       ctACCCATTCATGGCAATATCTCTCTTGCCGGTGACAGCAACGTCAAGCGATGGGCGTTGCGCTGCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttttt                                             <  1:689623/149‑1 (MQ=255)
                                                                                                         aCCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGc                                           >  2:237654/1‑149 (MQ=255)
                                                                                                         aCCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGGCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGc                                           <  2:237524/149‑1 (MQ=255)
                                                                                                           ccATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCt                                         >  1:64784/1‑149 (MQ=255)
                                                                                                                cAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGTTGGTCGTTGCGCTCCATATTGTATTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGTCGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGttat                                    <  2:72698/149‑1 (MQ=255)
                                                                                                                 aGGTCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTGCCTGttattt                                  <  1:526448/149‑1 (MQ=255)
                                                                                                                    gCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACTCCGACTTTGATGACTCGGCTGTTTTTTGCCAGttatttat                                >  2:896070/1‑149 (MQ=255)
                                                                                                                          tctctcTTGCAGGTGAATGCAACGTCTAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTGCTTGTTTGAATTACTGGATAGCCCAATTGATTCGTACGACGCCGAGTGTGATGAGTCGGCTTTTTTTGGCCTGTTATTTATGAGCgg                          >  2:138748/1‑148 (MQ=255)
                                                                                                                               ctTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTAcc                     >  1:1119677/1‑149 (MQ=255)
                                                                                                                                ttGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTAccc                    >  2:536331/1‑149 (MQ=255)
                                                                                                                                 tGCAGGTGAATGCTACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTACTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCt                   <  1:273807/149‑1 (MQ=255)
                                                                                                                                         aaTGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTTTCAGCGTCTACCCTTTAAGAGt           >  2:340619/1‑149 (MQ=255)
                                                                                                                                           tGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTcc         >  1:257239/1‑149 (MQ=255)
                                                                                                                                            gCAACGTCAAGCGATTGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCa        <  2:310477/149‑1 (MQ=255)
                                                                                                                                            gCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCCGCGTCTACCCTTTAAGAGTCCa        >  2:963035/1‑149 (MQ=255)
                                                                                                                                              aaCGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCCCAATTGATTCGTACTACGCCGACTTTGATGAGTCGGCGTTTTTTTGCCTGTTATTTATCAGCGTCTGCCCTTTAAGACTCcccc      >  2:797345/1‑149 (MQ=255)
                                                                                                                                                  tCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAAt  >  1:372609/1‑149 (MQ=255)
                                                                                                                                                  tCAAGAGATTGTCGTTGCGCTCCATATCGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTACGAGTCCACCCAAt  <  2:680958/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTGCTTTATGGTTGCATCGTAGTAAATGGTTGTAACAAAAGCAATTTTTCCGGCTGTCTGTATACAAAAACGCCGCAAAGTTTGAGCGAAGTCAATAAACTCTCTACCCATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAAT  >  NC_000913/1408999‑1409293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: