Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,833 G→C 6.9% intergenic (‑90/+21) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8330GC 6.9% 198.5 / 5.0 69intergenic (‑90/+21)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base C (2/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTG  >  NC_000913/1441688‑1441980
                                                                                                                                                 |                                                                                                                                                   
cttacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                                                                  >  2:31961/1‑149 (MQ=255)
  tacACATCCCGCCATCAGCATGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGTGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                                                                <  1:443433/149‑1 (MQ=255)
  tacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGTCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGc                                                                                                                                                <  1:1033543/149‑1 (MQ=255)
    cacaTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                                                              <  1:962506/149‑1 (MQ=255)
       aTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTGATTGGGGATTATCTGAATCCGCTCCCCTGGAATGCAgggg                                                                                                                                           >  1:69107/1‑149 (MQ=255)
          ccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                                                                        <  1:689269/149‑1 (MQ=255)
             ccATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctga                                                                                                                                     <  2:454452/149‑4 (MQ=255)
              cATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCa                                                                                                                                    >  2:954349/1‑149 (MQ=255)
                 cagcagGCTTAGCTCAAAAAACGCGGGTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATATAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                                                                 <  2:380843/149‑1 (MQ=255)
                   gcagGCTTAGCGCAACAACCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                               <  1:943275/149‑1 (MQ=255)
                   gcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                               >  2:273765/1‑149 (MQ=255)
                      ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                            <  2:106781/149‑1 (MQ=255)
                      ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                            <  1:435503/149‑1 (MQ=255)
                      ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                                                            <  1:495183/149‑1 (MQ=255)
                       gCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                                                                           <  2:873867/149‑1 (MQ=255)
                              gcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                                                                    >  2:627475/1‑149 (MQ=255)
                                aacaaACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgtt                                                                                                                  >  2:636944/1‑149 (MQ=255)
                                aacaaACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgtt                                                                                                                  <  1:1167693/149‑1 (MQ=255)
                                        cgGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                                                                          <  2:723888/149‑1 (MQ=255)
                                              cTTTCTTCATTGGGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCGGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                                                                    <  2:1063273/149‑1 (MQ=255)
                                               tttCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                                                                   <  1:262043/149‑1 (MQ=255)
                                                ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCt                                                                                                  >  1:887792/1‑149 (MQ=255)
                                                     cATTGTGTTGATCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTTGTGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                                                                             <  2:880727/149‑1 (MQ=255)
                                                     cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTc                                                                                             >  1:1031549/1‑149 (MQ=255)
                                                      aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                                                            >  1:1154877/1‑149 (MQ=255)
                                                       ttGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCa                                                                                           >  1:1150854/1‑149 (MQ=255)
                                                        tgtgGTTGTCAATTACAGTTTCTGACTCATGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                          <  1:250971/149‑1 (MQ=255)
                                                        tgtgGTGCGCAATTACAGTTTCTGACTCAGGACTATTTTCAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                          <  1:555546/149‑1 (MQ=255)
                                                              tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCt                                                                                    >  1:314230/1‑149 (MQ=255)
                                                                 cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                                                                 <  1:352746/149‑1 (MQ=255)
                                                                      aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                                                            >  1:598676/1‑149 (MQ=255)
                                                                       cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                           >  1:973855/1‑149 (MQ=255)
                                                                       cAGTGTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                                                                           <  1:610963/149‑1 (MQ=255)
                                                                          tttCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                                                                        <  2:567881/149‑1 (MQ=255)
                                                                              tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                                                                    <  1:350628/149‑1 (MQ=255)
                                                                               gACTGAGGACTATTTTAAGAATTGAGGATGATTGGTCATTGGGGATGATCTGAATCAGCTCCCCTGGATTGGAGGGTAGCGGAAAGATTAAACCAGTTGGTTGGTGCAGGTGTCGGCGTTGTCCAGATTGATTATGTGTTGAAGCGGAg                                                                   >  2:1154352/1‑149 (MQ=255)
                                                                                aCTCATAACTATTTTAAGAAAAGAGGATGAAAGGTCATTGGGGATTAGCTCAATCAGCACCCCGGGAATGCAGGGGAGCGGCAAGATTAACCCAGTTCGTTCGGGCAGGTTTCGCCGTTTTCAAGATTGCATTAGTTGTGCAGCGTAGt                                                                  >  2:1039046/1‑149 (MQ=255)
                                                                                aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                                                                  >  2:416647/1‑149 (MQ=255)
                                                                                  tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCt                                                                >  1:825728/1‑149 (MQ=255)
                                                                                  tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTCCTTAAGTTTTGCAGCGTAGTCt                                                                >  2:246085/1‑149 (MQ=255)
                                                                                   cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                               >  2:591235/1‑149 (MQ=255)
                                                                                     ggAATATTTTAAGAATATAGGATGAAAGGGCATTGGGGATTATCAGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                                                             <  2:501831/149‑1 (MQ=255)
                                                                                        cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa                                                          >  1:107098/1‑149 (MQ=255)
                                                                                           ttttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc                                                       >  1:912064/1‑149 (MQ=255)
                                                                                              tAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg                                                    >  1:193319/1‑149 (MQ=255)
                                                                                                 gAATAGAGGATGAAAGGTCCTTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa                                                 <  2:225555/149‑1 (MQ=255)
                                                                                                      gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                            >  1:33167/1‑149 (MQ=255)
                                                                                                        ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTATTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTCAGAAATACTGGTCAGAGCTTc                                          >  1:1120923/1‑149 (MQ=255)
                                                                                                         gATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTct                                         <  2:598676/149‑1 (MQ=255)
                                                                                                          aTGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctg                                        <  2:1150854/149‑1 (MQ=255)
                                                                                                               aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTc                                   <  1:31961/149‑1 (MQ=255)
                                                                                                                gggcaagggggAGTAGCTGAATCACCTCCCCTGCCATGCCGGGGAGCGGCAAGATTAAACCACATCGTTCGGGCAGGCGGCGCCTTTTTCCAGATTGCTTAAGGTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCa                                  <  2:944201/142‑1 (MQ=255)
                                                                                                                 gTCATTGGGGATTATCTGCATCAGCTCCCCTGGAATGCAGGGGAGCGGCACGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAg                                 <  1:665452/149‑1 (MQ=255)
                                                                                                                  tCATTGGGGATTATCTGAATCAGCTCCCCTCGAATGCAGGGGAGCGACAAGATTAAACCAGTACGTTCGGGCAGTTTACGCATATTTCAAGATTGCTTAAAATTACCACCGTAATCTGAAAAATACTGGTAATAGCTTCTGCTCTCacg                                >  2:772075/1‑147 (MQ=255)
                                                                                                                        gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGcc                          <  1:954349/149‑1 (MQ=255)
                                                                                                                          gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAACTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTg                        >  2:835632/1‑149 (MQ=255)
                                                                                                                          gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTg                        <  2:107098/149‑1 (MQ=255)
                                                                                                                                     tCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaa             >  1:737238/1‑149 (MQ=255)
                                                                                                                                       aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc           <  1:636944/149‑1 (MQ=255)
                                                                                                                                       aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGGAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc           >  1:1166235/1‑149 (MQ=255)
                                                                                                                                        gCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACa          >  2:1165762/1‑149 (MQ=255)
                                                                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGCTTGCTTATGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTGCTGCTGTCAGGAATGCCTGGTGCCCGGtttaca          >  2:949229/1‑144 (MQ=255)
                                                                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTATTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGATCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACa          >  2:615372/1‑149 (MQ=255)
                                                                                                                                           ccccTGGAATGCAGGGGCGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTATCCAGcc       >  2:1030903/1‑148 (MQ=255)
                                                                                                                                            cccGGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAc      >  1:660325/1‑149 (MQ=255)
                                                                                                                                             ccTGGAAGGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACg     >  1:703544/1‑149 (MQ=255)
                                                                                                                                              cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgct                                      <  2:1027773/115‑1 (MQ=255)
                                                                                                                                              cTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgct                                      >  1:1027773/1‑115 (MQ=255)
                                                                                                                                               tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGtt   <  1:180452/149‑1 (MQ=255)
                                                                                                                                                ggAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGTATGCCTGGTGCCCGGTAAACAGCACGTtg  >  2:721828/1‑149 (MQ=255)
                                                                                                                                                ggAATGAAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGGTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTtg  >  2:812953/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTTACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTG  >  NC_000913/1441688‑1441980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: