Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,765,166 T→A 8.5% intergenic (‑44/+56) ydiH ← / ← menI uncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,765,1660TA 8.5% 123.5 / 2.9 47intergenic (‑44/+56)ydiH/menIuncharacterized protein/1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base T (28/15);  new base A (2/2);  total (30/17)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAG  >  NC_000913/1765020‑1765314
                                                                                                                                                  |                                                                                                                                                    
cTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTa                                                                                                                                                    <  1:399820/149‑1 (MQ=255)
       tttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCa                                                                                                                                             <  2:1178909/149‑1 (MQ=255)
        ttCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTAAACGGTGGTATACTGTGCAg                                                                                                                                            <  1:1164330/149‑1 (MQ=255)
           aGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGGTGGGTGGGATCAAGTTGAGTAGACATAGCAACCTCGCTTTTAGACAAGACCTGCTCAGTATACCACCGTTTACTGTGCAGATa                                                                                                                                         >  1:203262/1‑149 (MQ=255)
                tttGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGAc                                                                                                                                    >  1:533169/1‑149 (MQ=255)
                                 gTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTc                                                                                                                   >  1:246556/1‑149 (MQ=255)
                                  tGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTca                                                                                                                  <  2:284378/149‑1 (MQ=255)
                                  tGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTca                                                                                                                  >  1:747366/1‑149 (MQ=255)
                                         ttGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACtttt                                                                                                           <  2:67274/149‑1 (MQ=255)
                                           tctcagttCACGACGTAAAAATTCAATGGCCAGCTGGGGGGGATCAATTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGGCACACTTTTCt                                                                                                         <  2:777890/142‑1 (MQ=255)
                                            ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTg                                                                                                        <  2:747366/149‑1 (MQ=255)
                                            ctctGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTg                                                                                                        <  1:509293/149‑1 (MQ=255)
                                                       gCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTc                                                                                             >  2:1023442/1‑149 (MQ=255)
                                                         gTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTcac                                                                                           <  2:199765/149‑1 (MQ=255)
                                                            aaaaTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACaaa                                                                                        >  2:906402/1‑149 (MQ=255)
                                                               aTTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATg                                                                                     <  2:14617/149‑1 (MQ=255)
                                                                ttCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATgg                                                                                    <  1:679898/149‑1 (MQ=255)
                                                                  cAATGCCCAGCGGGGTGGGATCCCGATGATTACTCATATCATCCTCGCGTATAGACAAGAACAGCACAGTATACCACCGGTTACTCTGCAGATACTGCCCAAAAGCAATAGGCGTCACACTTTTCTGCTGACAACGTCACAAAATGGCg                                                                                  <  2:765914/149‑1 (MQ=255)
                                                                               ggtgggATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTg                                                                     >  2:738702/1‑149 (MQ=255)
                                                                                    gATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACgca                                                                >  1:133702/1‑147 (MQ=255)
                                                                                    gATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGaa                                                                <  1:141906/149‑1 (MQ=255)
                                                                                      tCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACa                                                              >  2:723446/1‑149 (MQ=255)
                                                                                      tCAAGTTGAGTAGACAAAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACAGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACATTTCTGGTGACAACGTCACAAAATCGCGGTCGTCAATCGTGACGaaaa                                                              >  1:698208/1‑147 (MQ=255)
                                                                                         aGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACAAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGca                                                           >  1:997778/1‑149 (MQ=255)
                                                                                          gTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGcac                                                          >  1:1126812/1‑149 (MQ=255)
                                                                                             gAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACaaa                                                       >  1:1175905/1‑149 (MQ=255)
                                                                                              tgTAGTCATAGCATGCTCGCTTTTAGACAAGACCTGCACAGTATACCAACGTTTACTGTGCAGATAATGTCCAAAAGCAATATGCGTCAGACTTTTCTGGTGACATCGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACTAAc                                                      <  2:1142661/148‑1 (MQ=255)
                                                                                               gTAGAAATAGCATCCTCGCTTTTAGACAAGACCTGCTCAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACg                                                     <  2:297985/149‑1 (MQ=255)
                                                                                                tAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACCACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGc                                                    >  1:437898/1‑149 (MQ=255)
                                                                                                              tCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGCACAGCACAAACGCCCTTTCTCATCGaa                                      >  1:254706/1‑149 (MQ=255)
                                                                                                                gCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAACCGCCCTTTCTCATCGAAGa                                    >  2:957327/1‑149 (MQ=255)
                                                                                                                gCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGa                                    >  1:1023809/1‑149 (MQ=255)
                                                                                                                 cTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAACCGCCCTTTCTCGTCGAAGAt                                   >  1:1099566/1‑149 (MQ=255)
                                                                                                                        aCAATACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCACTTTCTCATCGAAGATTTCAATc                            >  1:259121/1‑149 (MQ=255)
                                                                                                                        aCAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATc                            >  2:847387/1‑149 (MQ=255)
                                                                                                                         cattatcTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                          <  1:973635/128‑1 (MQ=38)
                                                                                                                         cattatcTGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCAt                                          >  2:973635/7‑134 (MQ=38)
                                                                                                                           aGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGc                         <  2:997778/149‑1 (MQ=255)
                                                                                                                               cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAgc                     >  2:252686/1‑148 (MQ=255)
                                                                                                                               cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGa                     >  1:629502/1‑149 (MQ=255)
                                                                                                                               cTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGa                     >  1:276040/1‑149 (MQ=255)
                                                                                                                                tGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGAc                    >  2:144718/1‑149 (MQ=255)
                                                                                                                                tGCACAGTAAACGGTGGTATACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGATGATTTCAATCTGCCAGAc                    >  2:7009/1‑149 (MQ=255)
                                                                                                                                     aGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGCCCTGGt               >  1:1039214/1‑149 (MQ=255)
                                                                                                                                            caccGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGa        >  2:925917/1‑149 (MQ=255)
                                                                                                                                                gTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGACCCg    >  1:387065/1‑149 (MQ=255)
                                                                                                                                                gTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCg    >  1:209553/1‑149 (MQ=255)
                                                                                                                                                  ttACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCgag  <  1:738702/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTAGTTGTTTCAGACGTTTGAGATATTGGGCAGGTGAAAGATTGCTCTGGTCACGGCGTAAAAATTCAATGGCCAGCTGGGTGGGATCAAGTTGAGTAGACATAGCATCCTCGCTTTTAGACAAGACCTGCACAGTATACCACCGTTTACTGTGCAGATAATGACCAAAAGCAATATGCGTCACACTTTTCTGGTGACAACGTCACAAAATGGCGGTCGTCAATCGTGACGAACAGCACAAACGCCCTTTCTCATCGAAGATTTCAATCTGCCAGACCTGGTGACGCGAACCGAG  >  NC_000913/1765020‑1765314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑