Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 5.6% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC 5.6% 195.1 / 4.5 72intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base C (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

TGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAC  >  NC_000913/1865538‑1865829
                                                                                                                                                |                                                                                                                                                   
tGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGc                                                                                                                                                 <  2:904487/149‑1 (MQ=255)
 gTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACGAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCCCAACAGTAATGCAACACAGGGCGTGACGcc                                                                                                                                                >  2:986870/1‑149 (MQ=255)
  ttttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACGTAATTGAATCATTTTTTGTTGCATTCTTTCCGCCCAGGTTTTGCCACACCGTAATCCAAAAAAGGGCGTTACGccc                                                                                                                                               >  2:1083030/1‑149 (MQ=255)
     tGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGt                                                                                                                                            <  2:617683/149‑1 (MQ=255)
      gcgcTGACTGGCTGATTCGCCCATATACCATGTTCCCGGCGCTACGGCTGGCAGTGAGACATCGCCACTTAATTTAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACGACCGTAATGCAAAACATGTCGTGACGCCCTGtt                                                                                                                                           <  2:126008/149‑1 (MQ=255)
      gcgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                                                                           <  2:716298/149‑1 (MQ=255)
       cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                                                                          <  1:857856/149‑1 (MQ=255)
       cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCAATTTTTGGTGCATTGTTTCCTCCCCGGTATTGCACCAACGTAATGCAAAACCGGGCGTGACGCCCTGttt                                                                                                                                          >  2:9966/1‑149 (MQ=255)
        gcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGGCCTACGGCTGGCAGTGAGACATCGCCACTAAATAGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                         <  1:1114755/149‑1 (MQ=255)
          tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgc                                                                                                                                       <  1:636405/149‑3 (MQ=255)
            aCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                                                                     >  2:344555/1‑149 (MQ=255)
               ggCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCGCCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcac                                                                                                                                  <  2:732990/149‑1 (MQ=255)
                gCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                                                                 >  1:325171/1‑149 (MQ=255)
                  aTCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                               >  1:131766/1‑149 (MQ=255)
                   tCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                                                                              >  2:16326/1‑149 (MQ=255)
                    cTTCTCCAATATACCATGTTCCCTGCACTAGGGCTGGCAGTGAGAGATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGGACAAAAt                                                                                                                             <  2:710294/149‑1 (MQ=255)
                        gCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                         >  2:616936/1‑149 (MQ=255)
                         cccATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                                                                        >  1:4211/1‑149 (MQ=255)
                         cccATATACCATGTTCCCTGCCCTACGGCGGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCTTTGTTTCCTCCCAGGTATAGCACCACCGTAATGCAAAACAGGGATTGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                                                                        >  1:653767/1‑149 (MQ=255)
                          ccATATACCATGTTCCCTGCACTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                                                                       <  1:793829/149‑1 (MQ=255)
                              ataCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                                                                   >  2:726230/1‑149 (MQ=255)
                               taCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAAAGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAg                                                                                                                  <  1:965605/149‑1 (MQ=255)
                                aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                                 >  1:623964/1‑149 (MQ=255)
                                  cATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                               >  2:404549/1‑149 (MQ=255)
                                  cATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAAACATTTTTTGTTGCATTGTTTCCTCACAGGTATTGCACCACCGTAATGAAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                               >  2:810113/1‑149 (MQ=255)
                                     tttCCCTGCCCTAGGGCTGGCTGTGTGACATCGCGACTAAATTGAATCATTTTTTGTTGCTTTGTTTCGTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGGACAAAATGCCCTGGAAAGATGCAt                                                                                                            <  1:1035285/148‑1 (MQ=255)
                                      ttCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAtt                                                                                                           >  2:361391/1‑149 (MQ=255)
                                        cccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAt                                                                                                         >  1:705751/1‑149 (MQ=255)
                                          cTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                                                                       >  1:225563/1‑149 (MQ=255)
                                            gCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCACCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                     <  1:891948/149‑1 (MQ=255)
                                            gCCATACGGCTGTCAGTGAGACATCGCCACTAAATTGAATCATTTATTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACATTGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                     <  1:24760/149‑1 (MQ=255)
                                               cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                                                                  <  1:125486/149‑1 (MQ=255)
                                                tACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACAGTAATGCAAAACAGGGCGTGCCGCGCTGTTTTTATGCACAAAATGCACTGGAAAGATGCATTATAAGAAttt                                                                                                 >  1:805881/1‑149 (MQ=255)
                                                 aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTAGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTTTGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtttt                                                                                                >  2:276987/1‑148 (MQ=255)
                                                      tGGCAGTGAGACTTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                           <  1:613986/149‑1 (MQ=255)
                                                        gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                                                                         >  1:52620/1‑149 (MQ=255)
                                                         cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                                                                        >  1:1155231/1‑149 (MQ=255)
                                                               gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                                                                  <  2:544777/149‑1 (MQ=255)
                                                                   tCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                                                                              <  2:970225/149‑1 (MQ=255)
                                                                       cACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                                                                          <  2:1114816/149‑1 (MQ=255)
                                                                            aaTTGAATAATTTTTTGTGGCATTGGTTCCTCCCAGGTAATGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                                                                     <  2:816088/149‑1 (MQ=255)
                                                                             aTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                                                                    >  2:879944/1‑149 (MQ=255)
                                                                                    cATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                             <  2:1155231/149‑1 (MQ=255)
                                                                                    cATTTTTTGTTGCATTGTTTCCTCCCAGGTATAGCACAACCGTAATTCAAAACAGGGCGTGACGACCTGTTTTTATGCACAAAATGCACTGGAAAGATGCATTATCAGCATTTGTAGGAGATCCAGGTAGAAATCATGCAAGTCAAGGa                                                             >  1:410555/1‑149 (MQ=255)
                                                                                      ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                           >  2:331282/1‑149 (MQ=255)
                                                                                          ttgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATc                                                       <  1:98180/149‑1 (MQ=255)
                                                                                             ttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGCAATCAGGCCAGTCCAGGATTTATccac                                                    >  2:267331/1‑149 (MQ=255)
                                                                                                cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTGTATGCACAAAATGCCCTGGAAAGCTGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCAt                                                 >  2:1142716/1‑149 (MQ=255)
                                                                                                 aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCTTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGACAGTCCAGGATTTATCCACAAta                                                >  1:691535/1‑148 (MQ=255)
                                                                                                    gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCggg                                             <  1:361391/149‑1 (MQ=255)
                                                                                                     tttCCTCCCAGGTATTGCACCAGCGTAATGCAAAACAGGGCGTGACGCCCTGGTTTTATGCACAAAATGCCCTGGAAAGATGCATTTTCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGc                                            >  1:421206/1‑149 (MQ=255)
                                                                                                     tttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTTTGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGc                                            <  1:276987/149‑1 (MQ=255)
                                                                                                            ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCGGTCAGt                                     <  1:817409/149‑1 (MQ=255)
                                                                                                              aGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                   <  1:452642/149‑1 (MQ=255)
                                                                                                                gTATTGCACCACCGTATTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCGCGGTAGATATCAGGACAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACt                                 <  1:108688/149‑1 (MQ=255)
                                                                                                                  aTTTCACCACCGTAATGCAATACAGGTCGGGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATAATCAGAATTTGTAGGTGATCCCGGAAGAAATCTGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtc                               <  2:653767/149‑1 (MQ=255)
                                                                                                                   ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtca                              <  2:52620/149‑1 (MQ=255)
                                                                                                                    tGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcat                             <  1:810113/149‑1 (MQ=255)
                                                                                                                     gCACCACCGTAATGCAAAACAGGGCGTGACGGCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAGCAGATTTTGTAGGTGATCCCGGTAGAAATCAGACCAGTCCAGGATTTTTCCACCATCGGGCTGTCAGTAACTtcatc                            <  1:9966/149‑1 (MQ=255)
                                                                                                                        ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCCGTCAGTAACTTCATcaga                         <  2:705751/149‑1 (MQ=255)
                                                                                                                         caccGTAATGCAAAACAGGGCGTGCCGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGTATTTGTAGGTGATCCCGGTAGAAATCCGGCCATTACAGGAGTTATACCCAATCCGGCTGTCAGTAACTTCATcagac                        >  2:653177/1‑149 (MQ=255)
                                                                                                                         caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                <  1:158384/61‑1 (MQ=255)
                                                                                                                         caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                >  2:158384/1‑61 (MQ=255)
                                                                                                                         caccGGACTGCAAATCAGGTCGTGACGCCATGATTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTAGTAGGGGATCCCGGTAGAAATCAGGCCAGTCCAGGAGTTATCCACCATCGGGCTGTCAGTAACTTCATaagtc                        <  2:542711/149‑3 (MQ=255)
                                                                                                                              tAATGCAAAACAGGGCGTGACGCGCTGTTTTTATGGACAATATGCCCTGGAATTATGCATTATCTGAATTAGACGGTGATCGCGGTAGAAATCAGGCCAGTGCCGGATTTATCCTCCATCGGGCTGTCAGTATCTTCATCTGACAGACg                   <  2:579355/149‑1 (MQ=255)
                                                                                                                                aTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCCGGCCAGTCCAGGATTTATCCACCAGCGGGCTGTCAGTAACTTCATCAGACAGACggg                 >  1:601330/1‑149 (MQ=255)
                                                                                                                                aTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCCGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACggg                 <  2:721944/149‑1 (MQ=255)
                                                                                                                                 ataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACgggg                >  2:503158/4‑148 (MQ=255)
                                                                                                                                    aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATACCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGAAAGACGGGTGTa             >  2:613222/1‑149 (MQ=255)
                                                                                                                                      aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTAATCAGACAGACGGGTGTAGc           >  2:572208/1‑149 (MQ=255)
                                                                                                                                           ggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGACATCAGGCCAGTCCAGGAATTCTCCACCATCGGGCTGTCAGTACGTTCATCAGACAGCCGGGTGTAGCGTGCg      >  2:50763/1‑149 (MQ=255)
                                                                                                                                               tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAc  <  1:344555/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAC  >  NC_000913/1865538‑1865829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: