Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,362,665 A→C intergenic (‑39/+68) pheU ← / ← yjdC tRNA‑Phe/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,362,6650AC98.9% 273.3 / NA 91intergenic (‑39/+68)pheU/yjdCtRNA‑Phe/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (48/42);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGA  >  NC_000913/4362525‑4362806
                                                                                                                                            |                                                                                                                                             
tccacggAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGGTATCCGGGCGACGGGCCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAAttt                                                                                                                                       >  2:1329900/6‑149 (MQ=21)
  cGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCTAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGTGCTATCCGTGCTACGGGGTGCATTAAACCGTATTCTGCAGATCTCGTCACCCTAATTTCa                                                                                                                                     <  1:1070460/149‑1 (MQ=21)
  cGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCa                                                                                                                                     <  1:783174/149‑1 (MQ=255)
         gTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAg                                                                                                                              >  1:471882/1‑149 (MQ=255)
           ccGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCg                                                                                                                            >  2:3085/1‑149 (MQ=255)
            cGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGa                                                                                                                           <  2:625162/149‑1 (MQ=255)
             gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGAt                                                                                                                          >  2:48861/1‑149 (MQ=255)
                 atatGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCaa                                                                                                                      <  1:531155/149‑1 (MQ=255)
                      aaTTTGGTGCCCGGACTCGGAATCGAACAAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCGAACCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATACATCtccg                                                                                                                 >  2:187714/1‑146 (MQ=18)
                        tttGGTGCCCGGACTCGGAAAAGAACCAAGGACACGGGGATTTTCAATCCACTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATATCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTa                                                                                                               >  1:1277118/1‑149 (MQ=255)
                         ttGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                                                                                                              <  1:661381/149‑1 (MQ=255)
                           ggTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTaa                                                                                                                                          >  1:940195/1‑119 (MQ=255)
                           ggTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTaa                                                                                                                                          <  2:940195/119‑1 (MQ=255)
                                 cGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCgg                                                                                                      >  1:1356178/1‑149 (MQ=255)
                                  ggACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGc                                                                                                     <  2:965638/149‑1 (MQ=255)
                                      tCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAAt                                                                                                 <  2:320186/149‑1 (MQ=255)
                                      tCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAAt                                                                                                 <  2:1356178/149‑1 (MQ=255)
                                        ggAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAACCCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCg                                                                                               >  1:1234204/1‑149 (MQ=255)
                                        ggAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCg                                                                                               >  2:1420642/1‑149 (MQ=255)
                                        ggAATAGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAACGCGATTCAACTGCTTAAGATAGCGGCAAATCg                                                                                               >  2:531822/1‑149 (MQ=255)
                                           aTCGAACCAAGGTCACGGGGATTTTCAATCCCCTTCTCTACCGACTGAGCTATCCGGGCAACGGGGATAATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGctt                                                                                            <  1:439830/149‑1 (MQ=37)
                                            tCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGctta                                                                                           >  2:677227/1‑148 (MQ=255)
                                             cGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTATGATTGCGGCAAATCGcttct                                                                                          >  1:923788/1‑149 (MQ=255)
                                               aaCCAAGGACACGGGGATTTTCAATCCCCTGCTCTCCCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCttt                                                                                        >  1:910685/1‑149 (MQ=255)
                                               aaCCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACCTCTCGTCACCATAAGTTCAGTAAATGCGAATCAACGGCTTAAGATTGCGGCAAATCGctaatgt                                                                                        >  2:803295/1‑144 (MQ=21)
                                                aCCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCtttt                                                                                       >  1:636462/1‑149 (MQ=255)
                                                aCCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCtttt                                                                                       <  2:659645/149‑1 (MQ=255)
                                                 ccAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGGGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTc                                                                                      <  2:252243/149‑1 (MQ=255)
                                                  cAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCt                                                                                     <  2:1193/149‑1 (MQ=255)
                                                    aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt                                                                                   >  1:572371/1‑149 (MQ=255)
                                                    aGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCACTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt                                                                                   >  1:394519/1‑149 (MQ=255)
                                                     ggACACGGGTATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAATCCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgtg                                                                                  <  2:362068/149‑1 (MQ=255)
                                                      gACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTgtgt                                                                                 <  1:1197400/149‑1 (MQ=255)
                                                       acacGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGtt                                                                                >  1:921412/1‑149 (MQ=255)
                                                        cacGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGttt                                                                               >  2:1092417/1‑149 (MQ=255)
                                                        cacGGCGATTTGCAATCCCCGGCTCTACCGACTTCGCTATCCTTGCAACTGGGCGCATTAAACCGTAAGCTGCACATCTCGTCTCCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGTTTGCGGAAAATCGCTTCTTTTCTGTGttt                                                                               <  2:317676/149‑1 (MQ=255)
                                                         acGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTc                                                                              >  2:6608/1‑149 (MQ=255)
                                                          cGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCt                                                                             <  1:145515/149‑1 (MQ=255)
                                                          cGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACAGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAATATTGCGGCAAATCGCTTCTTTTCTGTGTTTCt                                                                             <  1:765475/149‑1 (MQ=255)
                                                          aggggATTGAAAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAACCCGTAATCTGCACCTCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCt                                                                             >  1:295520/2‑149 (MQ=38)
                                                           ggggATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCCCATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTGTGTGTTTCTg                                                                            >  2:354242/1‑149 (MQ=255)
                                                           ggggATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTg                                                                            >  1:168426/1‑149 (MQ=255)
                                                            gggATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGt                                                                           <  1:489042/149‑1 (MQ=255)
                                                            gggATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGt                                                                           >  1:1167596/1‑149 (MQ=255)
                                                              gATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTACTTTCAGTAAAAGCTATTCAACTGCTTATGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGtaa                                                                         >  1:1189080/1‑147 (MQ=255)
                                                              gATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCa                                                                         <  1:870331/149‑1 (MQ=255)
                                                               attgaaaaTCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCCCCTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAg                                                                        >  1:373196/7‑149 (MQ=38)
                                                               aTTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCATATCGCTTCTTTTCTGTGTTTCTGTCAg                                                                        <  2:572917/149‑1 (MQ=255)
                                                               aTTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAg                                                                        <  2:579234/149‑1 (MQ=255)
                                                               aTTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGCGCAACGGGGCCCATTAAACCGAAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCTACTGCTTAAGATAGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAg                                                                        >  1:33005/1‑149 (MQ=38)
                                                                  ttCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTc                                                                     >  1:1406686/1‑149 (MQ=255)
                                                                    cAATCCCCTGCTCTACCGACTGATCTATCCGTGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCaa                                                                   <  2:1289674/149‑1 (MQ=255)
                                                                    cAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACCTCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCaa                                                                   <  2:1127959/149‑1 (MQ=255)
                                                                    cAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTATGTTTCTGTCAGGTCaa                                                                   <  2:587681/149‑1 (MQ=255)
                                                                         cccTGCTCTACCGCCTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTGTGTGTTTCTGTCAGGTCAATGcac                                                              >  2:939466/1‑149 (MQ=255)
                                                                         cccTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACGCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGccc                                                              >  2:1323585/1‑147 (MQ=255)
                                                                         cccTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGcac                                                              <  1:829184/149‑1 (MQ=255)
                                                                          ccTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAACAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGcacc                                                             <  2:544591/149‑1 (MQ=255)
                                                                             gCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCaccacc                                                          <  1:1197624/149‑1 (MQ=255)
                                                                                ctACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTg                                                       <  1:1161584/149‑1 (MQ=255)
                                                                                 tACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTgg                                                      >  1:1228344/1‑149 (MQ=255)
                                                                                        tGAGCTATCCGGTCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTATAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGGTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGc                                               >  2:99584/1‑149 (MQ=255)
                                                                                          aGCTATCCGGGCAACGGGGTGCATTAAACCGTAATCTGCACATCTCGTCACCCAAATTTCAGTAAAAGCGATTCAACTGATTAAGATTGCGGCAAATCTCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCgg                                             >  2:1162986/1‑149 (MQ=255)
                                                                                          aGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCgg                                             >  2:594050/1‑149 (MQ=255)
                                                                                          aGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCgg                                             >  1:565556/1‑149 (MQ=255)
                                                                                          aGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCgg                                             <  1:48861/149‑1 (MQ=255)
                                                                                           gCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGGCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCggg                                            <  1:1153998/149‑1 (MQ=255)
                                                                                              aTCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGa                                         >  2:26792/1‑149 (MQ=255)
                                                                                               tCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGaa                                        >  1:1389345/1‑149 (MQ=255)
                                                                                                ccGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAg                                       <  1:1420642/149‑1 (MQ=255)
                                                                                                ccGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAg                                       <  1:705769/149‑1 (MQ=255)
                                                                                                 cGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAgc                                      >  2:1053070/1‑149 (MQ=255)
                                                                                                   ggCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAgcgc                                    >  2:1295169/1‑149 (MQ=255)
                                                                                                       aCGGGGCGCATTAAACCGTAATCTGCACATCTCGTAACCCTAATTTCAGTAAAAGCTATTCAACTGCTTAAGATTGCGGCTAATCGCTTCATTTCTTTGTTTATGTCATGTCATATCACCACCCTGGCGGCAGAGGGCGAAGCGCTGGa                                >  1:788507/1‑149 (MQ=255)
                                                                                                        cGGGGCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGat                               <  2:636462/149‑1 (MQ=255)
                                                                                                          gggCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGatat                             <  1:480284/149‑1 (MQ=255)
                                                                                                          gggCGCATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGGCAATTCACAACCATGGCGGCAGCTGGCGAAGCCCAGGatat                             >  2:181690/1‑149 (MQ=255)
                                                                                                             cgcATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCtt                          <  2:1206598/149‑1 (MQ=255)
                                                                                                               cATTAAACCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTcc                        <  1:1026152/149‑1 (MQ=255)
                                                                                                                    aaCCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAg                   >  1:250941/1‑149 (MQ=255)
                                                                                                                     aCCGTAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCTAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGc                  >  2:388432/1‑149 (MQ=255)
                                                                                                                      ccGTAATCTGCCCATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGcc                 >  2:75094/1‑149 (MQ=255)
                                                                                                                         tAATCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGAt              <  2:471882/149‑1 (MQ=255)
                                                                                                                           aTCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCggg                                            >  2:310208/1‑117 (MQ=255)
                                                                                                                           aTCTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCggg                                            <  1:310208/117‑1 (MQ=255)
                                                                                                                             cTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGc          <  1:3085/149‑1 (MQ=255)
                                                                                                                             cTGCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCATTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGc          <  1:803295/149‑1 (MQ=255)
                                                                                                                               gCACATCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATTTCTTCCGCCAGCCGATGTGCAg        >  1:838897/1‑149 (MQ=255)
                                                                                                                                  caTCTCGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAgtgt     >  1:462099/1‑149 (MQ=255)
                                                                                                                                     ctcGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGa  >  1:637324/1‑149 (MQ=255)
                                                                                                                                     ctcGTCACCCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCCGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGa  <  2:942506/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGTGTTTCTGTCAGGTCAATGCACCACCCTGGCGGCAGCGGGCGAAGCGCAGGATATCTTCCGCCAGCCGATGTGCAGTGTCGA  >  NC_000913/4362525‑4362806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: