Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,406,129 T→C intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,406,1290TC98.3% 345.9 / ‑5.2 119intergenic (+144/‑61)purA/nsrRadenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (54/63);  minor base A (2/0);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405983‑4406274
                                                                                                                                                  |                                                                                                                                                 
tAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtg                                                                                                                                                 <  1:13249/149‑1 (MQ=255)
   ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTgg                                                                                                                                              >  1:624252/1‑149 (MQ=255)
     cTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCt                                                                                                                                            >  2:89598/1‑149 (MQ=255)
      tGGTACGCCTGGTAGTTATTTTTGCTGACGGGCGAACTGTGTGATACATTGCTGTGTCTGGTAAGCCATTACGCTATCCGAAACAGTGTTAAATCCTCGCTTTTTTCCTTCCCAGAACTGAAATAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                           <  2:1078489/149‑1 (MQ=255)
      tGGTACGCCTGGCAGATTTTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACATTGTTAAATCCTCGCTTTTTTCCTTACCCGAACTGAAAGAAATTAGCGCCACAGCCTGTGGCTg                                                                                                                                           >  2:1293630/1‑149 (MQ=255)
      tGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                           <  2:600906/149‑1 (MQ=255)
      tGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATACGACACAGTGTTAAATCCTCGCTTTTTTCATTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                           >  2:786810/1‑149 (MQ=255)
        gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                         >  2:150079/1‑149 (MQ=255)
        gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGt                                                                                                                                         >  1:1128205/1‑149 (MQ=255)
         tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGtt                                                                                                                                        <  2:877146/149‑1 (MQ=255)
          aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGttt                                                                                                                                       <  2:325383/149‑1 (MQ=255)
          aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTAAACGAACTGAAATAAATTAGCGAAACAGCATGTTGCTgtgtt                                                                                                                                       >  2:703644/1‑145 (MQ=255)
            gacTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTTCGCTATCCGTCACAGTGTTAAATCCTCGCTTTTTTGCTTCCCCGATCTGAAATAAATTAGGGACACATCCTGTGGCTGGTTTat                                                                                                                                     <  2:1280298/147‑1 (MQ=255)
             ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatc                                                                                                                                    <  1:47086/149‑1 (MQ=255)
              cTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                   <  1:1433679/149‑1 (MQ=255)
              cTGGCAGATATTTTGCCTACCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAACTTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                   <  2:1005835/149‑1 (MQ=255)
                 gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                <  1:1153223/149‑1 (MQ=255)
                 gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                >  1:576162/1‑149 (MQ=255)
                 gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                <  2:1212192/149‑1 (MQ=255)
                      tatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCt                                                                                                                                            <  2:856875/132‑1 (MQ=255)
                      tatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCt                                                                                                                                            >  1:856875/1‑132 (MQ=255)
                        ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                         >  2:627719/1‑149 (MQ=255)
                         tttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGCCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                        <  2:918086/149‑1 (MQ=255)
                            gcctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCATCGCTTTTTTCCTTCACCGAACTGAAATAAATAAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATgga                                                                                                                     >  1:1058103/1‑147 (MQ=255)
                              ctgccGGGCGAACAGTCTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACACTGATAAATCCTCGCTTTTTTCCTACCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCATTATAAATTTAtt                                                                                                                   >  1:371981/1‑149 (MQ=255)
                               tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttt                                                                                                                  <  2:87937/149‑1 (MQ=255)
                                   gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTc                                                                                                              <  2:836582/149‑1 (MQ=255)
                                     gCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTccc                                                                                                            >  2:1196938/1‑149 (MQ=255)
                                     gCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATAATCAATATAAATGTATTTTTTacc                                                                                                            >  2:248678/1‑149 (MQ=255)
                                          cAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                                       >  2:30103/1‑149 (MQ=255)
                                               tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtt                                                                                                  <  2:524579/149‑1 (MQ=255)
                                               tgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtt                                                                                                  >  1:849860/1‑149 (MQ=255)
                                                gATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGCACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCttt                                                                                                 >  1:984160/1‑149 (MQ=255)
                                                 tttcATTGGTGTGTGGTGTAAGACATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGTACTGTAATTTATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                                                                                <  1:402168/146‑1 (MQ=255)
                                                 aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                                                                                >  1:372009/1‑149 (MQ=255)
                                                 aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                                                                                <  1:1231558/149‑1 (MQ=255)
                                                    cATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTTTCATCAATATAAATGTATTTTTTCCCGATTTCACTTTTGAg                                                                                             >  1:1005220/1‑149 (MQ=255)
                                                      ttGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCATCGCTTTTTTCCAGCCCCGAACAGAAATAAATTATCGACACATCATGTCACTGTTTTAACATCAATATAAATGTATTTTTTCCCGCTTTCCCttttcattt                                                                                           >  2:1316559/1‑144 (MQ=255)
                                                         cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGATATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCACTTTTGAGGTTGa                                                                                        >  1:1142050/1‑149 (MQ=255)
                                                           gtgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtg                                                                                      <  2:1224004/149‑1 (MQ=255)
                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCATTATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     >  1:198158/1‑149 (MQ=255)
                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     >  1:1098549/1‑149 (MQ=255)
                                                            tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                                                                     >  1:444385/1‑149 (MQ=255)
                                                             gtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGTACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                    <  1:861025/149‑1 (MQ=255)
                                                             gtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                    <  1:187217/149‑1 (MQ=255)
                                                             gtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                    <  1:375335/149‑1 (MQ=255)
                                                                gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAg                                                                                 <  1:415515/149‑1 (MQ=255)
                                                                 ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGt                                                                                <  1:61172/149‑1 (MQ=255)
                                                                 ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGt                                                                                >  1:1442419/1‑149 (MQ=255)
                                                                     aGCCATTACGCTATCCGACACAGTGTTAATTCGTAGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                                                                            <  2:493590/149‑1 (MQ=255)
                                                                       ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAATTGTATTTTTTCCCGATTTCCCTTTGGAGGTTGATGTGATGTTAACGa                                                                          >  2:112095/1‑149 (MQ=255)
                                                                       ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                          <  1:673290/149‑1 (MQ=255)
                                                                        cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                                                                         >  2:1154752/1‑149 (MQ=255)
                                                                          ttACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGtt                                                                       <  1:98733/149‑1 (MQ=255)
                                                                           tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                      <  2:1128205/149‑1 (MQ=255)
                                                                           tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAAATGAAATAAATTAGCGACACAGCCTGTGGCTGTTTTATCATCAATATAAATGTATTTTTTCCCGATATCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                      <  1:1273831/149‑1 (MQ=255)
                                                                            aCGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTc                                                                     <  1:220668/149‑1 (MQ=255)
                                                                              gCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCAc                                                                   >  2:193819/1‑149 (MQ=255)
                                                                              gCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCAc                                                                   <  1:627719/149‑1 (MQ=255)
                                                                               cTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACt                                                                  <  1:715310/149‑1 (MQ=255)
                                                                                      acacAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacg                                                           >  1:660750/1‑149 (MQ=255)
                                                                                        acaGTGTTAAATCCTCTCTTTTTTCCTTCCACGAACTTTAATAAATAATCGACACAGCCTGTGGCTTGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTACCGAGTTTCACTgattacgga                                                         <  2:259421/149‑1 (MQ=255)
                                                                                          aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggatt                                                       >  2:401406/1‑149 (MQ=255)
                                                                                          aGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattaaggatt                                                       >  1:629450/1‑149 (MQ=255)
                                                                                             tttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACATGCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattacg                                                    <  1:32831/148‑1 (MQ=255)
                                                                                              ttAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTCGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                   <  2:508072/149‑1 (MQ=255)
                                                                                                aaaTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgc                                                 <  2:48870/149‑1 (MQ=255)
                                                                                                  aTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGGTGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcgc                                               <  2:401233/149‑1 (MQ=255)
                                                                                                  aTCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgcgc                                               <  1:438722/149‑1 (MQ=255)
                                                                                                   tCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCt                                              <  1:1196938/149‑1 (MQ=255)
                                                                                                     cTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACTAGTTTCACTGATTACGGATTACGTGCGCTGa                                            >  1:316671/1‑149 (MQ=255)
                                                                                                     cTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGa                                            <  1:1464426/149‑1 (MQ=255)
                                                                                                      tCGCTTTTTTCCTTCCCCGAACTCAAATAAATTAGCGACACACCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTCCAGTTAACCAGTTTCACTGATTACGGATTACGTGCGCTGaa                                           >  1:1284126/1‑148 (MQ=255)
                                                                                                       cGCTTTTTTCCTTCCCAGAAATGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATc                                          <  2:975482/149‑1 (MQ=255)
                                                                                                        gCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAATTCCCTTTTGAGGTTGTTGTGCAGGTATCGAGTTTCACAGATTTAGGATTACGAGGGCGGATCt                                         >  2:670718/1‑149 (MQ=255)
                                                                                                          ttttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTAc                                       <  1:1176863/149‑1 (MQ=255)
                                                                                                          ttttttCCTTACCCGAAATGAAATAAATTAGCGACACCTCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTACCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTAc                                       <  1:786810/149‑1 (MQ=255)
                                                                                                           tttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACa                                      >  1:479126/1‑149 (MQ=255)
                                                                                                           tttttCCTTCACCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATAATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCATTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACa                                      <  1:752787/149‑1 (MQ=255)
                                                                                                                 cttccCCGAACTGAAATAAATTAGCGCCACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                                >  1:1020951/1‑149 (MQ=255)
                                                                                                                 cttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACTTGCGCTGATCTACATGGCGt                                >  2:337858/1‑149 (MQ=255)
                                                                                                                 cttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                                >  2:1350451/1‑149 (MQ=255)
                                                                                                                  ttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGGTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTc                               <  2:508954/149‑1 (MQ=255)
                                                                                                                    ccccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCAt                             >  1:986176/1‑149 (MQ=255)
                                                                                                                      ccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                            >  1:482169/1‑116 (MQ=255)
                                                                                                                      ccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGGGCAGTTAACGAGTTTCACTgattac                                                            <  2:482169/116‑1 (MQ=255)
                                                                                                                       cGAACTTAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                          <  2:1044754/149‑1 (MQ=255)
                                                                                                                       cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGc                          <  2:1442419/149‑1 (MQ=255)
                                                                                                                           cTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGa                      <  2:1154504/149‑1 (MQ=255)
                                                                                                                            tGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGaa                     <  2:1098549/149‑1 (MQ=255)
                                                                                                                                ataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                 >  2:560550/1‑149 (MQ=255)
                                                                                                                                 taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGGGGAGGTGCAGTTAACGAGTTTCTCTGATTACGGTTGTCGTGCGCGGAACTACATGGCGGGATGGCCAGAgggggg                >  1:1388748/1‑147 (MQ=255)
                                                                                                                                  aaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCACTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTTCGCTGATCTACATGGCGTCATTGACAGAAGGGAgg               >  1:1277651/1‑149 (MQ=255)
                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  1:669244/149‑1 (MQ=255)
                                                                                                                                       aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc          <  2:1268440/149‑1 (MQ=255)
                                                                                                                                           acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGTATTACGTGCGCTTATCTACATGGCTTCATTGCCAGAAGGGCGGATGACCAGt      >  1:735895/1‑149 (MQ=255)
                                                                                                                                            cacaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTa     >  1:1050769/1‑149 (MQ=255)
                                                                                                                                             acaGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAt    <  1:533395/149‑1 (MQ=255)
                                                                                                                                              caGCCTGTGGCTGGTTTATCATAAATATAAATGTATTTTTTCCCGATATCCCTTTTGAGGTTGATTTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCTCTGATCTACATGGAGTCATTGACAGAAGGTCTGATGACCAGTAtt   >  2:707052/1‑149 (MQ=255)
                                                                                                                                               aGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTAttt  >  2:656353/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTT  >  NC_000913/4405983‑4406274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: