Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,019,460	G→A	V359I (GTC→ATC)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,019,460	0	G	A	100.0%	236.6 / NA	79	V359I (GTC→ATC)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/45);  total (34/45)

AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605                                                                                                                                                  |                                                                                                                                                  aaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                <  1:433089/149‑1 (MQ=255)  aGTGGCATTCCGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGGGGTCAGCAGCTGGCCGCTTCCCAGCAACAGCATAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTGCCCAtctc                                                                                                                                               <  2:302445/149‑1 (MQ=255)    gggCATTCATTTAGGGCAAAGCAACATCAGTGGAGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACACCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCtt                                                                                                                                             <  2:545999/148‑1 (MQ=255)      gCATTCATTAATGGCAAACCAACATCAGTGGCGAAAGCTTTAGTTGTCAGCAGCAGGACGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                           <  2:814325/149‑1 (MQ=255)       cATTCAGTTAGGGCAAAGCAACATCTGTGGCGAAAGCTTTAGGGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAAGCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                          <  1:1075399/149‑1 (MQ=255)        aTTCAGTTAGGGCAAATCAACATCAGTGGCGAAAGCTTTAGTGGGCAGCAGCAGGCCGCGTCCCAGAAACAGCAAAGCCAACGCACAGCAAACCATCAACCTGTGGCGGGGGACGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         <  1:143504/149‑1 (MQ=255)        aTTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         >  1:772968/1‑149 (MQ=255)          tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                                                                       <  2:1202672/149‑1 (MQ=255)             gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    <  2:185386/149‑1 (MQ=255)                aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGACAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 >  2:1235686/1‑149 (MQ=255)                 gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                                                                                <  1:608688/149‑1 (MQ=255)                  ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATAAACCTCTAGCGGAGGAAGACGACGATACGCTTCCGGTACCCATCACATTACAAtggacatta                                                                                                                               >  2:992539/1‑143 (MQ=255)                   gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCACCGCCCAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCGCTTTACAAGGGCgtgtca                                                                                                                              >  2:1150747/1‑147 (MQ=255)                   gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                              >  2:270959/1‑149 (MQ=255)                     aaaGCAACATCAGTGCCGATAGCTTTATTGGTCTCCAGCAGGCCGCTTCCCACCACCCGCAACGCCAACGCACATCAAACCAAGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACa                                                                                                                            <  1:1304471/149‑1 (MQ=255)                          aaCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCaa                                                                                                                       <  1:643828/149‑1 (MQ=255)                                   ggCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAGCGCACAGCAAACCATGAACCACTGGAGGGGGAACACCACGATACGCTACCGGTTCCCATCTCTTTTCAAGGGCGTGTAAGAGGCAACAGCGGCGt                                                                                                              >  2:154873/1‑149 (MQ=255)                                   ggCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAGCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGt                                                                                                              <  1:249993/149‑1 (MQ=255)                                    gCGAACGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCACAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGATGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCACCAGCGGCGtt                                                                                                             <  1:1218354/149‑1 (MQ=255)                                    gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                                                                             <  1:992539/149‑1 (MQ=255)                                      gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  2:43013/1‑149 (MQ=255)                                      gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  1:1444493/1‑149 (MQ=255)                                         aGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAACGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGGGTAACAGGCCACCGCGGCGTTGatat                                                                                                        >  1:1354248/1‑149 (MQ=255)                                            tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                                     >  1:687566/1‑149 (MQ=255)                                               aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGCGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  2:46782/149‑1 (MQ=255)                                                gTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                                                                                                 <  2:171068/149‑1 (MQ=255)                                                 tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                                                                                                >  1:1438822/1‑149 (MQ=255)                                                       gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                                                                          <  1:1275403/149‑1 (MQ=255)                                                          gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCTCAGCAACCCATGATCCTCTGGCGGGGGATGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                       <  1:134542/149‑1 (MQ=255)                                                           cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      <  2:1214318/149‑1 (MQ=255)                                                            agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGCTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                     >  1:34963/1‑149 (MQ=255)                                                            agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCGTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                     <  2:1415904/149‑1 (MQ=255)                                                             ggCCGCTTCCCAGCACCAGCAAAGCCAACGCACAGCCAACCATGAACCTCTGGCGGGGGAAGACGCCGATCCGCTTCCGGTTCCCATCTCTTTCGCAGGGCGTGTACCAGGCAACAGCGGCGGTGATATTTTCGCCGTACGTCAGAGGt                                                                                    >  1:104553/1‑149 (MQ=255)                                                             ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt                                                                                    <  2:1426443/149‑1 (MQ=255)                                                              gCCGCTACCCAGCAACAGCAAAGCCAACGCACAGCACACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGGCAGAGGTa                                                                                   <  1:1284232/149‑1 (MQ=255)                                                               ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                                                  <  2:1444493/149‑1 (MQ=255)                                                                      ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa                                                                           >  1:80025/1‑149 (MQ=255)                                                                          caacagcaaAGCCAACCCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCATCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                                                                       <  2:777083/149‑1 (MQ=255)                                                                           aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                                                                      >  1:1398054/1‑149 (MQ=255)                                                                             cagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgcg                                                                    >  2:1057866/1‑149 (MQ=255)                                                                              agcaaAGCCAACGCACAGCAATCCCTGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGAGTGTACCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   <  2:1440071/149‑1 (MQ=255)                                                                              agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   <  2:510019/149‑1 (MQ=255)                                                                                caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGGTGATATTTTCGCCTAACGTGAGAGGTAGCACCGTAATCCGCGTcg                                                                 >  2:1441235/1‑148 (MQ=255)                                                                                 aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  1:949473/115‑1 (MQ=255)                                                                                 aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  >  2:949473/1‑115 (MQ=255)                                                                                  aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCttt                                                               >  1:519206/1‑149 (MQ=255)                                                                                   aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCtttt                                                              >  1:251387/1‑149 (MQ=255)                                                                                    gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                             >  1:1404482/1‑149 (MQ=255)                                                                                     ccAAGGCACAGCAAACCATGAACCTGTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc                                                            <  2:765269/149‑1 (MQ=255)                                                                                      cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           <  2:772968/149‑1 (MQ=255)                                                                                      cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           >  2:873062/1‑149 (MQ=255)                                                                                        aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCg                                                         <  2:919811/149‑1 (MQ=255)                                                                                            acaGCAAACCATGACCCTCTCGCGGGGGAAGCCGACGATACGCATCCGGCTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCttt                                                     <  1:11895/149‑1 (MQ=255)                                                                                             caGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGGTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttg                                                    >  2:217278/1‑149 (MQ=255)                                                                                               taaaaCCATGAACGTCTGGCGGGTGAAGACTACGATACGCTTCCGGTTCTCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCTCGTCTTTTCCCCGCTttgtt                                                  <  2:1278047/147‑1 (MQ=255)                                                                                               gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgtt                                                  <  1:270959/149‑1 (MQ=255)                                                                                                cAAACCAGGACCCGCTGGCGGGGGAAGACGCCGAGACGCGTCCGGTTCCCATCTCTCTACAAGGGCGTGTCACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTGTTCCCCGCTttgttg                                                 <  2:956121/149‑1 (MQ=255)                                                                                                  aaCCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGGTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCCCCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcg                                               >  1:1380234/1‑149 (MQ=255)                                                                                                    ccATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCt                                             <  2:612524/149‑1 (MQ=255)                                                                                                     cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                            >  2:850926/1‑149 (MQ=255)                                                                                                     cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAACCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                            >  1:66420/1‑149 (MQ=255)                                                                                                     cATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGGGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                            <  2:64903/149‑1 (MQ=255)                                                                                                       tGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCaa                                          <  2:122831/149‑1 (MQ=255)                                                                                                            ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCTTTGTTGCGCTCAAGACGc                                     <  1:913755/149‑1 (MQ=255)                                                                                                                ggCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGa                                 >  2:133653/1‑149 (MQ=255)                                                                                                                       gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg                          <  2:1138800/149‑1 (MQ=255)                                                                                                                        aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                         <  1:1235686/149‑1 (MQ=255)                                                                                                                             gacgaTACGCGTCCGGGTCCCATCTCTTTACAAGGGGGTGTAACAGGCAACCGCGGCGTTGATATTTTCGCCTAACGGGTGTGGTTGCTCCGTAATCCGCGTGTTTTCCCCGCTTTGGTGCGCTCTAGAGGCAGGAGAATTAGcgggta                    >  2:263724/1‑144 (MQ=255)                                                                                                                                    cGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGGCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTa             <  2:1438822/149‑1 (MQ=255)                                                                                                                                     gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAg            <  1:873062/149‑1 (MQ=255)                                                                                                                                       ttCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCg          >  2:611560/1‑149 (MQ=255)                                                                                                                                        tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGa         <  2:66420/149‑1 (MQ=255)                                                                                                                                           ggTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCATTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGacac      <  2:687566/149‑1 (MQ=255)                                                                                                                                            gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCACCAGCGGCGTTGATATTTTCGCGTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGCCGGAGGATAATTAGCCGCTAAGCAGTAGCGacacc     >  2:303135/1‑148 (MQ=255)                                                                                                                                            gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca     >  2:1333298/1‑149 (MQ=255)                                                                                                                                            gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca     <  1:43013/149‑1 (MQ=255)                                                                                                                                             ttCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAg    >  2:81989/1‑149 (MQ=255)                                                                                                                                               cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCTTTGTTGCGCTCAAGAAGCAGGATAATTAGCCGATAAGCAGTAGCGACACAggt  >  1:1306939/1‑148 (MQ=255)                                                                                                                                               cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGGTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  >  1:1092849/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑