BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,238	T→A	5.8%	intergenic (+16/‑131)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,238	0	T	A	5.8%	862.0 / 8.9	283	intergenic (+16/‑131)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base T (137/115); new base A (20/4); total (164/119)
Fisher's exact test for biased strand distribution p-value = 8.41e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

    aCCTCCCTTACCTGGAGTGGGTCGGGGGTTCGTTCCCTTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                   <  1:5924/150‑1 (MQ=14)

    aCCTCCCTTACAAGGAGGGGGTCGGCTGTTCGATCACGTCAT‑CACCCACCACTCGGGTCGTTATCTCAGTTGTTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGTGTCGAAAACTGAAATACACACCAGTTTTAACAAAAAACTCAGa                                                                                                                                                   >  2:3128983/1‑150 (MQ=11)

    aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                   >  2:4299874/1‑150 (MQ=255)

    aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGa                                                                                                                                                   >  2:410790/1‑150 (MQ=255)

         ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGGTa                                                                                                                                              >  1:4870333/1‑150 (MQ=255)

          cTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTaa                                                                                                                                             <  1:605579/150‑1 (MQ=255)

               aaGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTg                                                                                                                                        >  1:932913/1‑150 (MQ=255)

               aaGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTg                                                                                                                                        >  1:2417007/1‑150 (MQ=255)

               aaGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTg                                                                                                                                        >  2:5513495/1‑150 (MQ=255)

                    gggggTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGATTTAACATCAAACTCAGATGATAAGCGTGAAGGa                                                                                                                                   >  1:4140715/1‑150 (MQ=255)

                       ggTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACAAAGATGTTAATCGTGAAGGATaa                                                                                                                                >  1:123616/1‑150 (MQ=255)

                           ggcggTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                            >  1:199058/1‑150 (MQ=255)

                           ggcggTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                            >  1:4609770/1‑150 (MQ=255)

                           ggcggTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGAAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAAACTGCACGACCCACCAGTTTTAACATCAAAAACAGATGTTAAGCGTGAAGGATAAAGtt                                                                                                                            >  1:979478/1‑150 (MQ=11)

                            ggggTTCGCTCCCGTCATCCCCCCACCA‑GCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTg                                                                                                                           <  1:6269815/148‑1 (MQ=11)

                               gTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcgc                                                                                                                        <  2:2521549/150‑1 (MQ=255)

                               gTTCGATCCCGGCAT‑CCCCCACCCCTCGGGTCGTTCGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcgc                                                                                                                        <  1:3346895/150‑1 (MQ=255)

                                ttCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACGTCGAACTAAGATGTGAAGCGGGGAGCGTAACGGTGCGcc                                                                                                                       >  2:3399135/1‑150 (MQ=11)

                                 tCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                      <  1:1044548/150‑1 (MQ=255)

                                   gATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACCGATGTTAACCGGGAAGGATAACGTTGCGCCAga                                                                                                                    >  2:2130217/1‑149 (MQ=255)

                                   gATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCGCAGGTGGTAGAGCCGTTGACGTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGCCCCACCCGGTTTAACCTCAAACGCAGATGGTAAGCGTGAAGGAGAACGTTGCGCCacc                                                                                                                    >  1:3166320/1‑148 (MQ=14)

                                    aTCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                   >  2:4487719/1‑150 (MQ=255)

                                     tCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACATTGCGCCAGCaa                                                                                                                  >  1:1551501/1‑150 (MQ=255)

                                     tCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATGAAAAAAAGTTGTTAAGCGGTAAGGATAACGTTGCGCCAGCac                                                                                                                  >  1:2864116/1‑149 (MQ=14)

                                     tCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAAGAAGTTGTTAAGCGTGAAGGATAACGGTGCGCCAGCaa                                                                                                                  >  1:792720/1‑150 (MQ=14)

                                       ccGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTTAACATCAAAATCAGATGTTAATAGTGAAGGATAACGTTGCGCCAGCaaag                                                                                                                >  1:3230185/1‑148 (MQ=14)

                                       ccGTCAA‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGACTCCTGCACGACCCACCAGTTTTAACATCACACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACg                                                                                                                <  1:3915622/150‑1 (MQ=255)

                                            at‑caCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCg                                                                                                           <  2:5896462/150‑1 (MQ=255)

                                                 cccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAgg                                                                                                       >  2:5401728/1‑150 (MQ=255)

                                                  ccaccaCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAggg                                                                                                      >  2:5427290/1‑150 (MQ=255)

                                                             gTCGTTAGCTCAGTTTTTATAGCAGTTGACTTTTAATCAATTTTTCGCATGTTCGAATCCTGCACGACACACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaagcg                                                                                           <  2:1840995/150‑1 (MQ=14)

                                                                     cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGCAGCGAGTCATcc                                                                                   >  1:6466515/1‑150 (MQ=255)

                                                                     cTCAGTGGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCCGTTTTACCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATcc                                                                                   <  2:5906050/150‑1 (MQ=255)

                                                                        aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGc                                                                                <  2:1765206/150‑1 (MQ=255)

                                                                         gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCa                                                                               <  2:574306/150‑1 (MQ=255)

                                                                            ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGa                                                                            >  2:3238309/1‑150 (MQ=255)

                                                                                 agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                            <  1:3046383/97‑1 (MQ=255)

                                                                                 agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAGCGTGAAGGATAACGtt                                                                                                                            >  1:246844/1‑97 (MQ=37)

                                                                                    aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGtt                                                                                                                                               <  2:6341316/75‑1 (MQ=255)

                                                                                      ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAAc                                                                                                                 >  2:3781941/1‑103 (MQ=255)

                                                                                      ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACGCAGAAGATAAGCGTGAAGGAAAACGTTGCGCAAGCAACGGCCCGAAGGGCGAGCGAAGCGAGTAATACAGCACCACCCACCAATc                                                                  >  2:6585240/1‑150 (MQ=14)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAACTCAGATGTTAAGCGTGa                                                                                                                                       >  2:6187019/1‑76 (MQ=25)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAg                                                                                                                                            >  2:1316565/1‑71 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                                       >  2:1158445/1‑76 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                                       <  1:6187019/76‑1 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                                       <  1:1158445/76‑1 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTCGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCATaaa                                                             >  2:6401068/1‑150 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             <  2:4958714/150‑1 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             <  2:932913/150‑1 (MQ=255)

                                                                                           ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTGAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                             >  2:4428443/1‑150 (MQ=255)

                                                                                            tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGa                                                                                                                                       <  1:5319048/75‑1 (MQ=255)

                                                                                               aaTCAATTGGTCGCAGGTTCTAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAtt                                                         <  2:2417007/150‑1 (MQ=255)

                                                                                                 tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                       >  1:5783888/1‑150 (MQ=255)

                                                                                                 tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                       >  1:1952611/1‑150 (MQ=255)

                                                                                                 tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                       <  1:4029156/150‑1 (MQ=255)

                                                                                                 tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                       <  2:2232254/150‑1 (MQ=255)

                                                                                                 tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCTGCACGACCCACCAATCTAAAAGATTgg                                                       >  1:4096700/1‑150 (MQ=255)

                                                                                                  ataTTGGTCTCAGTTTCGAATCCTGCACTACCCACCATTTTTAACATCAAACTCTGATGTTAAGCGTGAAGGTTAACGTTGGGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGc                                                      <  1:3636444/148‑1 (MQ=17)

                                                                                                   aaTTGGTCGCAGTTTCTATTCCTGCTCGACCCACCTTTTTTAAAATCAAACTCATTTTTTATGGTTGAAGGATATGTTTGCGCCTGCCACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTTAAGATTggtg                                                     <  1:1689763/150‑3 (MQ=12)

                                                                                                   aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                                    >  1:267414/1‑119 (MQ=255)

                                                                                                   aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCCATCGTAAAGTTTGGcg                                                     >  2:4885010/1‑149 (MQ=14)

                                                                                                     ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                   >  1:756363/1‑150 (MQ=255)

                                                                                                       ggTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                 <  2:166881/150‑1 (MQ=255)

                                                                                                       gctCGCAGGTCCGCCTCCGGCACGACCCACCTGTTTTAACATCAAACTAATATTTTTTGCGTGAAGGTTAAGGTTGTCCGAGCTTCGGCCCGTAGTTCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                 <  1:6131407/148‑1 (MQ=16)

                                                                                                         tCGCAGGTTCGAATCCTGCATGACCCACCAGTTTTAACATCAAACTCAGATGTGAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATCTTAAAGATTGGCCCCGAGt                                               >  2:1518922/1‑150 (MQ=255)

                                                                                                          cGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACAACAAGCTCAGAGGTGAATCGTGAAGGATAACGTGGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTCTGAAGGATTGGCCCCGAGtc                                              >  1:5538057/1‑149 (MQ=17)

                                                                                                            cAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaa                                            >  1:5735448/1‑150 (MQ=255)

                                                                                                             aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAGCCAGCACGACCCACCAATCTTCAAGATAGGCCCCGAGTaaaa                                           >  2:3092203/1‑150 (MQ=255)

                                                                                                             aGGTTCGAATCCTGCACGACCCACCAGATTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCATAAAGATTGGCCCCGAGTaaaa                                           <  2:5105999/150‑1 (MQ=255)

                                                                                                                 tCGAATCCTGCACGACCCACCAGTTTTAACATCTAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                       >  2:3563797/1‑150 (MQ=255)

                                                                                                                 tCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCCATCTTAAAGGTGGGCCCCGAGTAAAAATCt                                       >  2:210550/1‑150 (MQ=255)

                                                                                                                 tCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                       <  2:3967088/150‑1 (MQ=255)

                                                                                                                    aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                    >  1:3182974/1‑150 (MQ=255)

                                                                                                                    aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                    >  1:5662794/1‑150 (MQ=255)

                                                                                                                    aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAGTGGCCCCGAGTAAAAATCTTTc                                    >  1:2141090/1‑150 (MQ=255)

                                                                                                                    aaTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCCAAGCGAGTCATCCCCCACGACCCACCCAACAAACAGATTGGCCCCGAGGaaaaaacagac                                    >  2:5184373/1‑144 (MQ=17)

                                                                                                                     aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGGGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCGTTcg                                   >  2:327977/1‑149 (MQ=255)

                                                                                                                      tCCTGCACGACCCACCATTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAg                                  <  1:1423453/150‑1 (MQ=255)

                                                                                                                      tCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAg                                  <  1:3750514/150‑1 (MQ=14)

                                                                                                                           cACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAac                             >  2:1413271/1‑150 (MQ=255)

                                                                                                                              gACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAcc                          >  1:652404/1‑150 (MQ=255)

                                                                                                                                     caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                   >  2:3372156/1‑150 (MQ=255)

                                                                                                                                     caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                   <  1:3267064/150‑1 (MQ=255)

                                                                                                                                     caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                   >  1:4710548/1‑150 (MQ=255)

                                                                                                                                      aGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATggg                  <  1:2891818/150‑1 (MQ=255)

                                                                                                                                       gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                 >  1:4423324/1‑150 (MQ=255)

                                                                                                                                         tttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCg               <  2:6066489/150‑1 (MQ=255)

                                                                                                                                           tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt             >  2:3722826/1‑150 (MQ=255)

                                                                                                                                           tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt             >  1:3461602/1‑150 (MQ=255)

                                                                                                                                             aCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg           <  1:1063682/150‑1 (MQ=255)

                                                                                                                                               aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt         <  2:199058/150‑1 (MQ=255)

                                                                                                                                               aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt         <  2:4612596/150‑1 (MQ=255)

                                                                                                                                                tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCACCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCGTTCAGGTAACACCCGTATGGGTCGTTAGCTc        <  1:1839283/150‑1 (MQ=255)

                                                                                                                                                tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc        >  2:3012936/1‑150 (MQ=255)

                                                                                                                                                  aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTctg      >  1:5049955/1‑148 (MQ=255)

                                                                                                                                                  aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAg      >  1:5180938/1‑150 (MQ=255)

                                                                                                                                                    aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGtt    <  1:6293992/150‑1 (MQ=255)

                                                                                                                                                      tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg  >  1:2239685/1‑150 (MQ=255)

                                                                                                                                                      tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGAATGGGTCGTTAGCTAAGTTgg  >  1:4481881/1‑150 (MQ=255)

                                                                                                                                                      tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACACACCAATCTTAAAGATTGGCCCCGAGTAAAAATCATTCAGGTAACACCCGTATGGGTCGTTATCTCAGTTgg  >  1:4243366/1‑150 (MQ=255)

GAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCAT‑CACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG  >  NC_000913/781089‑781387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`