Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,226 T→G 9.9% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2260TG 9.9% 430.4 / 7.3 218intergenic (‑369/+126)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (135/25);  new base G (13/13);  total (171/47)
Fisher's exact test for biased strand distribution p-value = 2.45e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGG  >  NC_000913/915081‑915374
                                                                                                                                                  |                                                                                                                                                     
gATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgc                                                                                                                                                  >  2:6174002/1‑150 (MQ=32)
     gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTcc                                                                                                                                             >  2:3387269/1‑150 (MQ=17)
       gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                           >  2:4573366/1‑150 (MQ=32)
       gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCtt                                                                                                                                           >  2:3776684/1‑150 (MQ=32)
        cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTg                                                                                                                                          >  1:2548298/1‑150 (MQ=32)
        cTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTg                                                                                                                                          >  1:3952556/1‑150 (MQ=17)
         tGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTgc                                                                                                                                         >  1:1684144/1‑150 (MQ=17)
             ggCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGGTTGGCCGCCCCCTGCGCGCTCCTTGCGCCa                                                                                                                                     >  1:649831/1‑150 (MQ=17)
              gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                    >  2:3354892/1‑150 (MQ=32)
              gcgcGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAg                                                                                                                                    >  1:3244632/1‑150 (MQ=32)
               cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGt                                                                                                                                   >  1:5975852/1‑150 (MQ=17)
                gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGTACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCATGCGCCAGTg                                                                                                                                  >  1:5668236/1‑150 (MQ=12)
                gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTg                                                                                                                                  >  2:3743109/1‑150 (MQ=32)
                   ttttCCGGGGTTGTTACCCCCCGCCCGACCGGGGAGCGGTCCGGTATAGGTGGCGGGCACCGCGCCGGGGGGTTTTTGGCCGCAACACCGGGGGGGGGCTGGGGGGGGGGGCGTTGGCCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                               <  2:728343/150‑1 (MQ=2)
                        cAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATg                                                                                                                          >  1:4030519/1‑150 (MQ=38)
                        cAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCGTGCGCCAGTGGCAATATg                                                                                                                          >  1:2132890/1‑150 (MQ=255)
                         aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAatattt                                                                                                                         >  1:1293421/1‑148 (MQ=255)
                         aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCAATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                         >  2:6197812/1‑150 (MQ=255)
                         aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCAAATGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAAAATGt                                                                                                                         >  2:2902257/1‑150 (MQ=255)
                          gggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGtt                                                                                                                        >  1:2220556/1‑150 (MQ=40)
                           gtttGTTCCCGCGCGCCCGCCCGGGTAGCCGTATGGTATATGTCGCGGGCACCGCGCCGCGGCGTTTTTGTGCGCCAGCGCCGGGGGGTGCCGGGGGGGGGGGCGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                       <  2:3592630/147‑1 (MQ=14)
                           ggttGTTCCCGCCCGCCGGACCGGGTCGCCGAACGGTTAAAGTGGTGGGCACCGGGCCGGGGTTTTTTTGTTCGCTAGCGCCGGGGGGTGCTGGGGGGGGCGGCTTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTg                                                                                                                       <  2:5839029/147‑1 (MQ=9)
                           ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCATGGGGCGGCGGCGAGTGGCCCCCCCCAGCGCGCTCCGTGCGCCAGTGGCAATATGTTg                                                                                                                       >  1:4943768/1‑150 (MQ=14)
                           ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGGGGCAATATGTTg                                                                                                                       >  1:880363/1‑150 (MQ=255)
                           ggATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTGGGCCGCCCCCGGCGCGCGCCTTGCGCCAGTGGCAATATGTTg                                                                                                                       >  1:1316828/1‑150 (MQ=14)
                            gATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGTGGCAATATGTTGc                                                                                                                      >  2:566794/1‑150 (MQ=255)
                                ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGTGGCACTATGTTGCTTAg                                                                                                                  >  1:4157176/1‑150 (MQ=255)
                                  aCCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGGAGGGGGGGGCGGCGATTGGCCGCCCCCGGCGCGCTCCGTGCGCCAGTGGGCAGATGTTGGttttct                                                                                                                >  1:1132445/1‑146 (MQ=11)
                                  aCCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTTCTTAGCt                                                                                                                >  1:3601414/1‑150 (MQ=255)
                                    cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGGTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                              >  1:5237294/1‑150 (MQ=255)
                                     tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAt                                                                                                             >  2:1576555/1‑150 (MQ=255)
                                       gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTTGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTTCTTATCTCATGa                                                                                                           >  2:2642085/1‑150 (MQ=255)
                                       gcgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGa                                                                                                           >  1:4965747/1‑150 (MQ=255)
                                        cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGaa                                                                                                          >  2:651046/1‑150 (MQ=255)
                                             gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCACGGGCAATATGTTGCTTAGCTCAAGAAAGGAg                                                                                                     >  2:587502/1‑150 (MQ=255)
                                             gACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCTTGCGCCAGTGGCAATATGTTTCTTAGCTCATGAAAGGAg                                                                                                     >  1:2992744/1‑150 (MQ=255)
                                                ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGTGCGCCCCTGGCCAAAATTTGCTTATCTCAAGAAAGGAgcgc                                                                                                  >  1:1148194/1‑150 (MQ=14)
                                                 cGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCACTGGCAATAAGTTGCTTAGCTCATGAAAGGAGCGca                                                                                                 >  1:5786167/1‑150 (MQ=255)
                                                   gTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaac                                                                                               >  1:4326344/1‑150 (MQ=255)
                                                   gTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaac                                                                                               >  2:2564632/1‑150 (MQ=255)
                                                    tAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGATCCCGGGGGTGCAGGGGGCGGCGGCGTGTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAATGAGCGcaaca                                                                                              >  2:5127884/1‑150 (MQ=17)
                                                         gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTTGCCCCCCCCGGCGCGCACCGTGCGGCACTGGCAAAATTTTTCATAGCTCATGAAAGGAGCGCAACAAgaag                                                                                         >  1:4392256/1‑148 (MQ=17)
                                                         gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatg                                                                                         >  2:2205612/1‑150 (MQ=255)
                                                         gTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAATTGCCGCCCCCAGCGCGCTCCTTGCCCCAGTGGCAAAATTTTGCATAGCACAAGAAAGGAGCGCAACAAtatg                                                                                         >  1:4391958/1‑150 (MQ=17)
                                                                aTAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATc                                                                                  >  2:1224912/1‑150 (MQ=255)
                                                                aTAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCCGTGGCCAAATTGTGCTTATGGCATGAAAGGGGCGCCACAAGATGATGAATc                                                                                  >  1:155231/1‑150 (MQ=17)
                                                                 tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCATGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCa                                                                                 >  2:2012165/1‑150 (MQ=255)
                                                                 tAAGTCGAGGGCACCGCGCAGCGGCGATTGTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCGTGCGCCAGGGGCACGATGTTGCTTAGCGCGTGAGCGGCGCGCCACACGATGATGACTCa                                                                                 >  2:5159073/1‑150 (MQ=14)
                                                                     tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                             >  2:3207194/1‑150 (MQ=255)
                                                                       gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACatat                                                                           >  2:4366983/1‑150 (MQ=255)
                                                                        tgggCACCGGGCTGCGGCTTTTTTGTTCGCCTGTGCCGGGGGGTGCTGGGGGCGGGGGGGTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACAtata                                                                          <  2:3655166/149‑1 (MQ=21)
                                                                             aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaacaac                                                                     >  2:819078/1‑150 (MQ=255)
                                                                             aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAAACAACCAATaacaac                                                                     >  2:3989136/1‑150 (MQ=255)
                                                                                       ggtttttttGTTCGCCTGTGCGGGGGGGTGCTGGGGGGGGGGGTTTTGGGCGGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                           <  2:328574/145‑1 (MQ=25)
                                                                                       ggcgTTTTTGTTCGCCTGAGCCGGGGGGTGCTGGGGGGGGCGGCGATGGGCCGCCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAATATATAACAACATCTTaaaaa                                                           <  2:6566313/150‑1 (MQ=25)
                                                                                       ggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaa                                                           >  2:2060565/1‑150 (MQ=255)
                                                                                        gtttttttGGGCGCCCGCGCCGGGGGGGGCGGGGGGGGGGGGCGCGGGCCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaa                                                          <  1:1001273/146‑1 (MQ=255)
                                                                                        gcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaa                                                          >  2:101465/1‑150 (MQ=255)
                                                                                         cttttttGTCCGCCTGCGCCGGGGGGTGGTGGGGGCGGGGGCTTTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaaaaa                                                         <  1:3978488/147‑1 (MQ=255)
                                                                                          gATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                                        >  2:665801/1‑150 (MQ=255)
                                                                                            ttttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGACTCAACATATAACAACATCTTAAAAAAAGGc                                                      >  2:4863665/1‑150 (MQ=255)
                                                                                             ttggtgcGCCTGAGCCGGGGGGGGCTGGGGGGGGCGGCTTTGGGCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGcc                                                     <  2:4030519/144‑1 (MQ=255)
                                                                                                 ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCAGAc                                                 >  2:1373461/1‑150 (MQ=255)
                                                                                                 ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATAAGTTGCTTAGCTCAAGGAAGGAGCGCAACAAGATGATGTAACAACCTATAACAACAGCTTAAAAAAAGGCCTGAc                                                 >  2:5547708/1‑150 (MQ=25)
                                                                                                           cgggggggTGCTGGGGGGGGGGGGTTTGGCCCCCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAg                                       <  1:4838674/147‑1 (MQ=38)
                                                                                                            ccGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAgg                                      >  2:5458017/1‑150 (MQ=255)
                                                                                                              gggggTGCAGGGGGCGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAGGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGcc                                    >  2:2803836/1‑150 (MQ=255)
                                                                                                                 cgcgCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTc                                 >  2:56297/4‑150 (MQ=255)
                                                                                                                   tGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTg                               >  1:739444/1‑150 (MQ=255)
                                                                                                                    gcggggggcgggggcGATGGCCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGc                              <  1:4628776/147‑1 (MQ=38)
                                                                                                                    gCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGc                              >  2:2238436/1‑150 (MQ=255)
                                                                                                                      ggggggCGGCGGCTATGGGCCCCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCACCAAGCTGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGGCGTAATGTCAGGCCttttttta                            <  2:4102299/149‑7 (MQ=255)
                                                                                                                         gggCGGGGGCGTGGGGCCGCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                         <  2:1316828/150‑1 (MQ=255)
                                                                                                                         gggCGGCGGCGATTGGTCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                         >  2:5376453/1‑150 (MQ=255)
                                                                                                                         gggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCACGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                         >  1:3809809/1‑150 (MQ=255)
                                                                                                                           ggggcggccAATCGCCGCCGCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                       >  1:4339917/3‑150 (MQ=255)
                                                                                                                           ggggcggccAATCGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTCAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                       >  2:758353/3‑150 (MQ=255)
                                                                                                                           ggggcggccAATCGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                       >  2:1450085/3‑150 (MQ=255)
                                                                                                                           ggggcggccAATCGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCACATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                       >  1:3536655/3‑150 (MQ=39)
                                                                                                                           gcggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                       >  1:1386780/1‑150 (MQ=255)
                                                                                                                              ggggcgTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                    <  2:5288130/148‑1 (MQ=255)
                                                                                                                              gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                    >  2:1267472/1‑150 (MQ=255)
                                                                                                                              gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTCACATTACGCCAGGCCTTCTGCGAtaattaat                    >  2:3920365/1‑150 (MQ=255)
                                                                                                                              gcggcgAGTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                    <  2:4026823/150‑1 (MQ=255)
                                                                                                                                gggctttGGCCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATc                   <  2:1132445/145‑1 (MQ=255)
                                                                                                                                 gcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCAc                 >  1:5624396/1‑150 (MQ=255)
                                                                                                                                   gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCCGGCCTTCTGCGTTAATTAATCACGc               >  2:6090519/1‑150 (MQ=255)
                                                                                                                                   gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc               >  2:1832263/1‑150 (MQ=255)
                                                                                                                                   gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc               >  2:4315203/1‑150 (MQ=255)
                                                                                                                                   cttgggcccgcccgcTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAATAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg                <  2:5117559/143‑1 (MQ=255)
                                                                                                                                   cgtgggcccgccCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACg                <  2:6116672/143‑1 (MQ=255)
                                                                                                                                    tggggCCCCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCt              <  1:4533863/147‑1 (MQ=255)
                                                                                                                                        gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc          >  1:5813138/1‑150 (MQ=255)
                                                                                                                                        gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCAGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc          >  1:1271641/1‑150 (MQ=255)
                                                                                                                                         cccgccgCCCCCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc          <  2:2335776/149‑1 (MQ=255)
                                                                                                                                          ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc         >  1:2076122/1‑150 (MQ=255)
                                                                                                                                          ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc         >  1:1190050/1‑150 (MQ=255)
                                                                                                                                          ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc         >  2:1862704/1‑150 (MQ=255)
                                                                                                                                           cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca        >  2:3716671/1‑150 (MQ=255)
                                                                                                                                                ccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAgg   >  2:5008052/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGG  >  NC_000913/915081‑915374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: