Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,955 T→A 6.8% intergenic (+429/+111) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9550TA 6.8% 560.1 / 5.6 199intergenic (+429/+111)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base T (53/122);  new base A (15/2);  total (75/124)
Fisher's exact test for biased strand distribution p-value = 4.02e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

GTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCG  >  NC_000913/1286806‑1287104
                                                                                                                                                      |                                                                                                                                                     
gTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGct                                                                                                                                                       >  1:5335422/1‑150 (MQ=255)
gTCGAAGCTTCTCATCCTTCCCCGATTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGct                                                                                                                                                       <  2:6126774/150‑1 (MQ=255)
   gAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctcgc                                                                                                                                                    >  1:1389341/1‑148 (MQ=255)
    aaGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctctct                                                                                                                                                   <  2:1314379/150‑1 (MQ=255)
    aaGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTTATTTGGGATTCGTTTGAGATTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctctct                                                                                                                                                   <  2:4633142/150‑1 (MQ=11)
    aaGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctctct                                                                                                                                                   <  2:5879351/150‑1 (MQ=255)
     aGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCtctctc                                                                                                                                                  <  1:1731187/150‑1 (MQ=255)
     aGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCtctctc                                                                                                                                                  <  1:254349/150‑1 (MQ=255)
          ctccgccTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTATGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                             <  1:3521872/145‑1 (MQ=255)
          ctcATCCTTCCCCGCTTGGGCAGAATAATTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGa                                                                                                                                             <  2:2973262/150‑1 (MQ=255)
             aTCCTTCCCCGCTTGGGCATATTATTTGATTTCGTATTCGTTTGAGAATTCCGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                                          <  2:5242031/150‑1 (MQ=14)
             aTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGGTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTAACGCAGACGc                                                                                                                                          >  1:5178587/1‑150 (MQ=14)
                 ttCCGCGCTTGGGCAGTATATTTGTTTGCGTATTCGTTTGAGTTTTACGTGTATTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                      <  2:1237447/150‑1 (MQ=14)
                 ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                      >  2:6078444/1‑150 (MQ=255)
                 ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTAGCAAGCGGCAACGCTCACACGCTGACGCTcca                                                                                                                                      >  1:4945414/1‑148 (MQ=14)
                 gtCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGa                                                                                                                                      <  2:5596869/149‑1 (MQ=255)
                  tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTTGGGGAAGGATTACTCAGCGCAGCGCGCTTCGCCCTTCTGGTCCTTTCCTGCGGCAAAGCCCTCTCGCAGACGCTCGac                                                                                                                                     >  1:1655361/1‑149 (MQ=14)
                   ccccGCTTGGGCAGACTATTTGATTGCGGATTCGTTTGAGTATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                    <  1:4823010/150‑1 (MQ=255)
                       gCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                <  2:4854323/150‑1 (MQ=255)
                             gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                          <  2:3880914/150‑1 (MQ=255)
                             gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTAGCCCTTCGGGTCGTGGGCAGCGGCCACGCGCACTCCCGGGCGCGCGAGGCGTAccgtgg                                                                                                                          >  1:5567920/1‑146 (MQ=11)
                               ataaTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTAGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCCAAACATAGTc                                                                                                                        >  2:3580700/3‑150 (MQ=14)
                               aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGCGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                        >  2:4085552/1‑150 (MQ=255)
                                  atatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                     <  1:4402146/150‑1 (MQ=255)
                                   tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                    <  1:3340524/150‑1 (MQ=255)
                                         agtGCGGATTCGTGTGAGCAGTCCGGGGCGTTTGAACGTGATGGTGGGGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTGAGTCGAAGCTtctc                                                                                                              <  1:4277665/148‑1 (MQ=14)
                                         aTTGCGGATTCGTTTGAGCATTCCGGGGCTTTTGAAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtctc                                                                                                              <  2:3744110/150‑1 (MQ=255)
                                                agtCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                       <  2:4151270/148‑1 (MQ=255)
                                                  tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTacc                                                                                                     >  2:1986129/1‑150 (MQ=255)
                                                  gcGTTTGAGAATTCCGGGGCTTCTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                     <  2:4247071/149‑1 (MQ=255)
                                                        gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                               <  2:223403/150‑1 (MQ=255)
                                                        gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTCACGCAGACGCACCAGCCGAACCGAAGTCGAAGCTTAACAAACATCCCCGCaag                                                                                               >  1:1900104/1‑148 (MQ=14)
                                                        gagaATTCCGGGGCAATAGAAACTGAAGCTGGTGGCGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTACGGCTATTTTCCTGCCCCAAATCACTCTCTCAGACCATCCAGCCGAACCTTACTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                               <  2:5012497/150‑1 (MQ=9)
                                                         agtaTTGCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                              <  1:674185/147‑1 (MQ=255)
                                                         agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCACACGCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATACATCCCCCCCTgg                                                                                              >  1:4677235/1‑150 (MQ=14)
                                                         agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                              <  2:5347678/150‑1 (MQ=255)
                                                               ggttgtgttttGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                        <  1:4142683/143‑1 (MQ=255)
                                                                   ggCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                    <  2:3411443/150‑1 (MQ=38)
                                                                    gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                   <  2:2838541/150‑1 (MQ=38)
                                                                    gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCGCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                   >  2:5634422/1‑150 (MQ=38)
                                                                      ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                                 <  1:4599503/150‑1 (MQ=38)
                                                                         tGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACGCACTCTCGCTGACGCACGAGCCGAAACATAGTAGAAGCTTCTAATCCTTCCCCGCATGGGCAGCATAATTTATTg                                                                              >  1:6464305/1‑150 (MQ=11)
                                                                           aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACACTCGCAGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCATCCCCGCATGGGCCGCATATATGATTGCg                                                                            >  2:2612871/1‑150 (MQ=14)
                                                                            aaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCgg                                                                           <  2:3591090/150‑1 (MQ=255)
                                                                                  tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                                     <  1:747472/150‑1 (MQ=40)
                                                                                  gttcgttggGGGACTGATTACTCTGCGCTGCCCGGTCGGCCGTCCGGTCTGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGc                                                                     <  2:6466024/144‑1 (MQ=11)
                                                                                      ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                 <  1:5969206/150‑1 (MQ=255)
                                                                                      ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                 <  1:924733/150‑1 (MQ=255)
                                                                                      ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                 <  1:3987442/150‑1 (MQ=255)
                                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                <  1:5488705/150‑1 (MQ=255)
                                                                                        tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                               <  2:5335422/150‑1 (MQ=255)
                                                                                        tggGGGAAGGATGACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                               <  1:155628/150‑1 (MQ=255)
                                                                                         gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGagag                                                              <  1:2768332/150‑1 (MQ=255)
                                                                                           gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                            <  2:1655357/150‑1 (MQ=255)
                                                                                              aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                         <  1:755363/150‑1 (MQ=255)
                                                                                              aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                         <  1:2469983/150‑1 (MQ=255)
                                                                                              aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                         <  2:4493356/150‑1 (MQ=255)
                                                                                              aaGGATTACTCAGCGCTGCGCGCTTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                         <  1:2355337/150‑1 (MQ=255)
                                                                                               aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                        <  1:1446620/150‑1 (MQ=255)
                                                                                                ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  1:6561193/150‑1 (MQ=255)
                                                                                                 gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      <  2:3835547/150‑1 (MQ=255)
                                                                                                   ttACTCAGCGCCGCGCTCTTCGCCCTTCTGTTCGTTGCCGGCGGCATCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACt                                                    <  2:4077705/150‑1 (MQ=17)
                                                                                                    tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                   <  1:4560607/150‑1 (MQ=255)
                                                                                                    tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                   <  2:1558218/150‑1 (MQ=255)
                                                                                                    tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                   >  2:208577/1‑150 (MQ=255)
                                                                                                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGTGAGTTCAGGGACgtt                                                  >  2:5936082/1‑150 (MQ=255)
                                                                                                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGCTTGCGGATTCGGTGGGGAGGTCAGGGACgtt                                                  >  2:5935886/1‑150 (MQ=11)
                                                                                                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACttt                                                  <  2:2411459/150‑1 (MQ=255)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 <  1:3086844/150‑1 (MQ=255)
                                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 <  1:5895884/150‑1 (MQ=255)
                                                                                                       tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTg                                                >  2:3647735/1‑150 (MQ=255)
                                                                                                       tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGTGAGTTCAGGGACTTTTg                                                >  2:641913/1‑150 (MQ=14)
                                                                                                             cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                          >  2:3947015/1‑150 (MQ=255)
                                                                                                              tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                                         <  1:2435210/150‑1 (MQ=255)
                                                                                                                gtcgcTTCGCGCTTCTGTTCGTTTCTTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtg                                       <  2:6436781/148‑1 (MQ=14)
                                                                                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtg                                       <  1:1160387/150‑1 (MQ=255)
                                                                                                                  cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCg                                                                                                                                       >  2:6284744/1‑52 (MQ=35)
                                                                                                                  cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCg                                                                                                                                       <  1:6284744/52‑1 (MQ=35)
                                                                                                                     ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                                  <  1:5304894/150‑1 (MQ=255)
                                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                 <  1:3055892/150‑1 (MQ=255)
                                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTATTGAAAGTGAtggtggtg                                 <  1:3964062/150‑1 (MQ=255)
                                                                                                                      tCGCCCATCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                 <  1:4241142/150‑1 (MQ=11)
                                                                                                                        gCCCTTCGGGTCGTTGCCTTCGGCAACGCTCTCTCGCTGCCGCTAGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATTGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGATAGTGATGGTGGTggg                               <  1:3251979/150‑1 (MQ=14)
                                                                                                                        gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTAGTTTGTGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                               >  2:4797033/1‑150 (MQ=255)
                                                                                                                        gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                               <  2:1188697/150‑1 (MQ=255)
                                                                                                                         cgcTTCGGGTCGTTGCGTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                              <  1:2022050/148‑1 (MQ=255)
                                                                                                                          ccTTCGGGGCGTTGGGTGCGGCTACGCTCTCTCGCGGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTggggg                             <  1:3470791/150‑1 (MQ=11)
                                                                                                                            ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                           <  1:6394488/150‑1 (MQ=255)
                                                                                                                            ttCGGGTCGTTGCCTGCGGCAAAGCTCTCACGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCCTTCCCCGCATTGGCAGAATATTTTATTGCGGATTAGGTGGTGAGTTCAGGGACTTTTGAAAATTAAGGGGGTggggcgt                           >  2:3350529/1‑147 (MQ=11)
                                                                                                                                ggTCGTTGCCTGCGGCAAAGCTCTAACCCTGAAGCTCGAGCCGAACCTAAGTCGAAGCGTCTCAAACTTACCCCCATGGGCAGAAAAATTTATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTTGTGGGGGGAGGAt                       >  2:739661/1‑150 (MQ=14)
                                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                     <  1:1197580/150‑1 (MQ=255)
                                                                                                                                          cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcg              >  2:5439298/1‑150 (MQ=255)
                                                                                                                                             cGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg           <  2:5490201/150‑1 (MQ=255)
                                                                                                                                               gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcg         <  2:2740055/150‑1 (MQ=255)
                                                                                                                                                  aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTTATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcgc      >  1:5903377/1‑150 (MQ=255)
                                                                                                                                                     ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc   <  1:363194/150‑1 (MQ=255)
                                                                                                                                                      tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCTCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg  <  2:4014458/150‑1 (MQ=255)
                                                                                                                                                      tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg  <  2:1424739/150‑1 (MQ=255)
                                                                                                                                                      tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg  >  2:3133629/1‑150 (MQ=255)
                                                                                                                                                      tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGATTGCGGATTAGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCGCTTCg  >  1:4370279/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCG  >  NC_000913/1286806‑1287104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: