Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 7.3% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 7.3% 642.5 / 31.7 236noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (119/97);  new base A (8/9);  total (128/108)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 240 total aligned reads displayed.

CTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287000‑1287298
                                                                                                                                                     |                                                                                                                                                     
cTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACGCGCGCTCGCTGGCGCACg                                                                                                                                                       >  1:739891/1‑150 (MQ=255)
   ccccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                                    <  2:4907155/150‑1 (MQ=255)
      cGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGa                                                                                                                                                 <  1:3392960/150‑1 (MQ=255)
      cGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGa                                                                                                                                                 >  2:4532113/1‑150 (MQ=255)
         aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCGACGCTCTCTCGCTGGCGCTCGAGTCGAAcc                                                                                                                                              >  2:6450691/1‑150 (MQ=255)
          tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTACGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCt                                                                                                                                             >  2:5476703/1‑150 (MQ=255)
           gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCTCGAGTCGAAccat                                                                                                                                            >  2:769723/1‑148 (MQ=255)
             gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGaagcgagg                                                                                                                                          >  1:4714334/1‑144 (MQ=255)
             gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACGCGCACTCGCAGGCGCTCGACTCGAAccaagg                                                                                                                                          >  1:1457470/1‑146 (MQ=14)
             gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTAGAAAGTGATTGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACCCACACACGCTGGCGCACGACTCGAAACGTgg                                                                                                                                          >  2:2938674/1‑150 (MQ=14)
              cAGAATCTTTGATTGCGGAGTCGCTTGAGACTTCCGGGGCTTTTGACAGGGAGGGTGGTGGGGGAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         <  2:2034220/150‑1 (MQ=11)
              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         <  2:1261631/150‑1 (MQ=255)
              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCACGCGGGCGCTCGAGGCGAACCGTggg                                                                                                                                         >  2:6336456/1‑149 (MQ=14)
              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCACGCTGGCGCTCGAGTCGAACCGTGGt                                                                                                                                         >  2:6229336/1‑150 (MQ=255)
              cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGCTTGCGCTCGACTCGAACCTTGGt                                                                                                                                         >  2:3199148/1‑150 (MQ=255)
               aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                        <  1:3089023/150‑1 (MQ=255)
                            gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                           <  1:2834477/150‑1 (MQ=255)
                                  tCGCTTGAGAGTTCAGGGACTTTTGATAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTccc                                                                                                                     <  1:1276379/150‑1 (MQ=14)
                                         agagTTCAGGGACTTTTGATAGTGTTGGTGGTGGGGGAAGGATTACGCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                              <  1:6306711/150‑1 (MQ=255)
                                          gagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCTGGCCCGCGAGTCGAACCATGGTCGAAGCTTCTCATCCTAACCCGCATggg                                                                                                             >  1:4865155/1‑150 (MQ=14)
                                            tttCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                           <  1:1897809/149‑1 (MQ=21)
                                            gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                           <  1:2293173/150‑1 (MQ=255)
                                             ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCATCGCTGCGCGCTTCTCCCTTCGGGACGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATggggcg                                                                                                          >  2:4358621/1‑147 (MQ=14)
                                                aGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACCCGCACTCCCTGGCGCTCGACTCGAACCGTGGGCGTAGGGTCTCCACCTTCCCCCCAgggggcaaaa                                                                                                       >  2:3029206/1‑144 (MQ=14)
                                                 gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTATCATCCATACCCCCAAGGGCAGAata                                                                                                      >  1:5909131/1‑150 (MQ=14)
                                                      ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 <  2:1914204/150‑1 (MQ=38)
                                                       tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGAATGGGCAGAATATTTAAt                                                                                                >  2:4735480/1‑150 (MQ=255)
                                                                gATTGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  1:2975620/150‑1 (MQ=37)
                                                                  tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGt                                                                                     >  2:3743122/1‑150 (MQ=37)
                                                                  tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGATAGAGTCGAAACTTGGACGAATCTTCTCATCCTTCCACGCATCGGCAGAATAATTAATTTCGGATTCGt                                                                                     >  2:3605091/1‑150 (MQ=11)
                                                                   ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    <  1:3580700/150‑1 (MQ=37)
                                                                   ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAAATTTAAATGCTGAATCgat                                                                                    >  2:6553377/1‑148 (MQ=11)
                                                                    gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCATTGTCGAAGCATCACAAACTTACCCGCAAGGGCAGAATATTTAATAGAGGATTGGTTg                                                                                   >  2:5544382/1‑150 (MQ=11)
                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                >  2:4377824/1‑150 (MQ=40)
                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                <  1:6078444/150‑1 (MQ=40)
                                                                        tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCCTGGGCAGAAAATTTAAATTCGGAATCCTTGGGaa                                                                               >  2:5652577/1‑150 (MQ=11)
                                                                            ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAAGATTTAATTGCGGATTCGGTGGGGAGTTc                                                                           >  2:3153288/1‑150 (MQ=11)
                                                                              aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:496005/150‑1 (MQ=255)
                                                                                 gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGGAACGGGGTCGAAGCGTCTAGTCCATCACCGCAAGGGGAGGATTTTTAATGGCGGATTGGGTGGGAAGTTCAgggc                                                                      >  2:4061921/1‑149 (MQ=14)
                                                                                 gATGACTCAGCGCTCCGCGCTTAGCCCTTCGGGTCGTTGCCTGCTTCTTAGCTCTCTCGCTGGGGCTGGAGTCGAAACCTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                      <  1:1998971/150‑1 (MQ=14)
                                                                                  aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCGTGGTCGAAGCGTCTCATCCTTCCCCGCCTGGGCAGAATATTTAATTGCGGCTTCGTGGGGAAGTTCAGGGAc                                                                     >  2:6374790/1‑150 (MQ=14)
                                                                                    tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      <  1:6347100/67‑1 (MQ=33)
                                                                                    tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      >  2:1601473/1‑67 (MQ=33)
                                                                                     aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCtt                                                                                                                                            >  2:1282334/1‑76 (MQ=34)
                                                                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              <  2:1899795/150‑1 (MQ=255)
                                                                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATACTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGGAGTTCCGGGACTTTTgga                                                              >  2:1333094/1‑148 (MQ=14)
                                                                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTgga                                                              >  2:2337867/1‑148 (MQ=255)
                                                                                             cTGCGCGCTTCGCCCTTCGGGTCGTTACCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCAAGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                          >  1:4803330/1‑150 (MQ=17)
                                                                                              tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:681362/150‑1 (MQ=255)
                                                                                                cgcgcTTCGCCCTTCGGGTCGGTGGCGGCGGCTCCGCTCTGTCGCGGGCGCTCGTGTCGTAGCTCGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                       <  1:4108312/150‑1 (MQ=17)
                                                                                                 gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtgg                                                      >  2:3549524/1‑150 (MQ=255)
                                                                                                      tCGCCCGTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                 <  1:2612871/150‑1 (MQ=255)
                                                                                                         cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                              <  1:3230664/150‑1 (MQ=255)
                                                                                                            ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:519459/150‑1 (MQ=255)
                                                                                                             tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCGTCTCATCCTTCCCCGCAAGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          >  2:1087880/1‑150 (MQ=255)
                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                   >  2:2387756/1‑88 (MQ=38)
                                                                                                                  tCGTGGCCTGCGGCACCGCTCTCTCGCGGGATCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  2:5188592/150‑1 (MQ=17)
                                                                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                                   >  2:1643998/1‑150 (MQ=255)
                                                                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                                   >  2:5874767/1‑150 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                    <  2:5266574/52‑1 (MQ=35)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                   >  1:3631307/1‑85 (MQ=38)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                                <  1:4317431/88‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                                <  1:840142/88‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                                <  1:1640505/88‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  <  2:3960360/150‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  >  2:217072/1‑150 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  >  2:2571714/1‑150 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  >  2:4305449/1‑150 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                                <  2:152418/88‑1 (MQ=18)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                  >  2:1711433/1‑150 (MQ=255)
                                                                                                                      tggcTGCGGCAACGCTGTCTCGCTGTCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGATTATTTTATTGCGGATTCGTTGGTTAGTTCAGGGACTTTTGAAAGTTATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:5041233/147‑1 (MQ=14)
                                                                                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:1965930/150‑1 (MQ=255)
                                                                                                                       gCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             >  2:1856467/1‑121 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTCGAACCt                               >  2:1793431/1‑150 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             >  2:2708835/1‑120 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtgg                                                   >  2:5064545/1‑130 (MQ=255)
                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAc                                                                                                                                               <  1:1342322/37‑1 (MQ=40)
                                                                                                                          tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:5543237/150‑1 (MQ=255)
                                                                                                                          agCGGCCACGCTCACCCGCCGGCGCTCGAGTCCACCCCTGGTCGAAGCTTCTCATCCTTCCCCGCATTTGCAGAATATTTTATTGCGGCTTCGTTGGGAAGTTGTGGTACTTTTTAATGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:5628932/149‑1 (MQ=17)
                                                                                                                           gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATGCGAACCTTCg                            >  2:2775338/1‑150 (MQ=255)
                                                                                                                            cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATGGCGGATGCGTGGGGAAGTTCAGGGACTTTTGAAAGTGAGGGGGGTGGGGGAAGGATTCGCACCGTCGa                           >  2:871571/1‑150 (MQ=17)
                                                                                                                             ggCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          <  1:3690334/150‑1 (MQ=255)
                                                                                                                             ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          >  2:5776160/1‑150 (MQ=255)
                                                                                                                             ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTAGaa                          >  2:5416221/1‑150 (MQ=255)
                                                                                                                             ggCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTgg                                                                                  <  1:1377774/94‑1 (MQ=12)
                                                                                                                              gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg                         <  1:1711433/150‑1 (MQ=255)
                                                                                                                                aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                       <  2:1373915/150‑1 (MQ=255)
                                                                                                                                  cGCTCTCCCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     <  1:957621/150‑1 (MQ=255)
                                                                                                                                   gCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    <  1:3958707/150‑1 (MQ=255)
                                                                                                                                   gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    <  1:5932615/150‑1 (MQ=255)
                                                                                                                                     tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGt                                                           >  2:3236609/1‑109 (MQ=255)
                                                                                                                                        ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg               <  2:657179/150‑1 (MQ=255)
                                                                                                                                         tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATACTTCCCCGCAAGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGACGGc              >  1:1101039/1‑150 (MQ=18)
                                                                                                                                             tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCTTCCCCGCAAGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          >  1:1554875/1‑150 (MQ=255)
                                                                                                                                                  cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:6560383/150‑1 (MQ=255)
                                                                                                                                                  cTCGAGTCGAACCGTGGTCGCAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:6432632/150‑1 (MQ=255)
                                                                                                                                                   tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg    >  2:6084511/1‑150 (MQ=255)
                                                                                                                                                    cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt   <  1:2705576/150‑1 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  1:2498242/1‑150 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  1:4033343/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287000‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: