Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 6.3% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 6.3% 807.8 / 9.8 269intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (120/122);  new base A (20/6);  total (141/128)
Fisher's exact test for biased strand distribution p-value = 1.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428
                                                                                                                                                      |                                                                                                                                                       
aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                                                         <  2:3301010/150‑1 (MQ=255)
aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                                                         <  1:2500642/150‑1 (MQ=255)
 atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAGTGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                                                        <  1:6123607/150‑1 (MQ=255)
 atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                                                        <  1:4842013/150‑1 (MQ=255)
       actGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                  <  1:2273778/148‑1 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                  >  2:512203/1‑150 (MQ=255)
        aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                                                                 <  2:5576181/150‑1 (MQ=255)
           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATAAACAAAAAGAc                                                                                                                                              >  1:2014078/1‑150 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGCTTTCAAAAAATAATGAAAGTATTTGTTTTTAAATATCGAGAGAACCAGCACCAAAACcaca                                                                                                                                             >  2:3930438/1‑150 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAAAATAATTAAATTATTTTTTTTTTAATATAGAGATAAAGATCACaaaaaacaca                                                                                                                                             >  1:2907807/1‑145 (MQ=255)
                   gCATAACCGCCGCGCTGTGTGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTTTTTTTTTTTTAATATGGAGATAAAGATAAAAAAAAAGAGAATATGaa                                                                                                                                     >  1:1010979/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                                                                     <  2:1837142/150‑1 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATTAAATTATTTTTTTTTTAATTTAGATATTAAGATAAAAAAAAAGACAAGATTaa                                                                                                                                     >  1:254580/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGaa                                                                                                                                     >  1:675695/1‑150 (MQ=255)
                         ccgccgCGCGGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                               <  2:5000798/150‑1 (MQ=255)
                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgc                                                                                                                           >  2:503275/1‑149 (MQ=255)
                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                           <  2:4843546/150‑1 (MQ=255)
                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAAAATGAAAattattagg                                                                                                                           >  2:5250127/1‑147 (MQ=255)
                                gcTGTGCGTTTTCCAGCATCTGCTTTGTCGACACCACGAACATTTTCATAACCTGTCTTTTTTTTTCTATTAAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCgagga                                                                                                                        <  1:6578552/150‑1 (MQ=255)
                                      cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCCATTTAAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAAAGAAAATTATTCGAGGAGTGaaa                                                                                                                 >  2:6580758/1‑150 (MQ=255)
                                      cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                 <  2:5753853/150‑1 (MQ=255)
                                      cGTTGTTCAGCATCTGCTTGGTCGATACCACGAACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                 <  2:5284309/150‑1 (MQ=255)
                                       gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGGATTAAAAAAATAATGTAATTATTTTTTTTTAAAAAAAGAGAGAAAGAACACAAAAAAGCCAAAATGAAAATTTTTTGTGGAGTGTAAg                                                                                                                >  1:5938721/1‑150 (MQ=255)
                                        ttgttCAGCATCGGATTTGTCTATACCACCTAAATTTTCATAGCCTGTCTTTTTTTTTCTATTACAAAATATAATCAAATTATTTTTTTTTAAATATCTAGATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                                                               <  1:42396/150‑1 (MQ=255)
                                           ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                                                            >  2:1621215/1‑150 (MQ=255)
                                               gaaTCTGATTTGTCGATACCACTTAAATTTTCATATCCTTTCTTTTGTTTTCTATTTAAAATTATAATTAAATTTTTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                                                                        <  1:3838347/148‑1 (MQ=255)
                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTAGAGGAGTGAAAGGCaaaaaa                                                                                                        >  2:3252820/1‑150 (MQ=255)
                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTTAATATAGAGATTAAGATAAAAAAAAAAAAAAAAATAAAATTATTAGAGGTGTTAGAGGCaaaaaa                                                                                                        >  1:3365775/1‑150 (MQ=255)
                                                 aTCTTCTTTTTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  2:1682408/150‑1 (MQ=255)
                                                 aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  2:1102887/150‑1 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                                                     <  2:5923970/150‑1 (MQ=255)
                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATAAAAATAAAATATTTTTTTTTTAAAATAAAGATAAAAAAAAAAAAAAAAAAAAAATTAAAAAAATTAGAGGAGTTGAAGGGAAAAAAAACg                                                                                                     >  1:441854/1‑150 (MQ=255)
                                                      cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGGTTTGAAAATATAAATTAAATATTTTTTTTTTAATATATAGATAAAGAAAAAAAAAAACAAAAAAATGAAAATATTAGTGGAGTGTAAGGCAAAAAAAAGGcca                                                                                                 >  1:3152186/1‑149 (MQ=255)
                                                          gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAAAAAGAAAAAATTTTTTTTTAAATATAGAGATAACGATCAAAAAAAAGACAATAATAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCACCCg                                                                                             >  2:2504117/1‑150 (MQ=255)
                                                           tCGATACCAGTTACATTTTCATATCCTTTCGTTTTTTTTCTTTTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                            <  1:3780146/150‑1 (MQ=255)
                                                           tCGACACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                            <  2:5308329/150‑1 (MQ=255)
                                                             gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                                          <  2:4634055/150‑1 (MQ=255)
                                                              aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                         <  2:6164945/150‑1 (MQ=255)
                                                                  cACGTAAATTTTAATATCCTGTCTTTTTTTTTCTTTTACAAAATATAATGAAATTATTTTTTTTTAAATATCTATATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                     <  1:923795/150‑1 (MQ=255)
                                                                   aCGTACTTTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                    <  1:950257/150‑1 (MQ=255)
                                                                   aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGGTAGGGAAg                                                                                    >  2:4940864/1‑150 (MQ=255)
                                                                   aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCCAAAAAAAGGCCTCCCGATAGGGAAg                                                                                    >  2:3700087/1‑150 (MQ=255)
                                                                     gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                                  >  2:5221002/1‑150 (MQ=255)
                                                                        ctttttCATACCGTGTCTTTTTTTTTCTTTTACAAAATATATTGAAATTTTTTGTTTTTAAATATCTAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtt                                                                               <  1:6317052/148‑2 (MQ=255)
                                                                        cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  2:3189001/150‑1 (MQ=255)
                                                                        cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  2:821670/150‑1 (MQ=255)
                                                                        cATTTTCAAACCCGTTCTTTTTTTTTCTATTTAAAAATATATTAAAATTTTTTTTTTTAAAATATCTAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  1:5511998/150‑1 (MQ=255)
                                                                             tCATATCCTGTCTTTTGTTTTCTATTTAAAAATATAATGAATTTATTTTTTTTTAAATATCTAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                          <  1:3240808/150‑1 (MQ=255)
                                                                                    cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATTAAAATTATTCGAGGAGTGAAATGCAAAAAAAACGCCACCCGATAGGGAAGCCGTATCAAAGTGcgcg                                                                   >  2:339803/1‑150 (MQ=255)
                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              >  1:4680957/1‑150 (MQ=255)
                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              >  1:2113786/1‑150 (MQ=255)
                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                             >  1:1763883/1‑150 (MQ=255)
                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                             >  1:3007501/1‑150 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  2:4186330/1‑150 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATg                                                         >  1:4752257/1‑150 (MQ=255)
                                                                                                  cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                     >  2:4075693/1‑150 (MQ=255)
                                                                                                       tCAACAGATAATTAACTTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:4782752/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:5211632/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  2:1526460/1‑150 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  1:461443/1‑150 (MQ=255)
                                                                                                       acAAAATATAATCAAATTATTTCTTTTAAAATACCGAGATACCCATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACCGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  2:2636/149‑1 (MQ=255)
                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               >  2:1998086/1‑150 (MQ=255)
                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                              >  1:1907687/1‑150 (MQ=255)
                                                                                                          aaaTATACTGACATTTTTTGTTTTTATATAGCGAGATCACGATCACACCCACGACAATAGGAAAATTATTCGAGGAGTGAACGGCAAAACAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                             <  2:3007501/150‑1 (MQ=255)
                                                                                                           aaTATACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  2:5147260/150‑1 (MQ=255)
                                                                                                           aaTATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  2:4304700/150‑1 (MQ=255)
                                                                                                            atatAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                           <  2:2893307/150‑1 (MQ=255)
                                                                                                              ctaATGAAATTATTTGTTTTTAAATATCGCGATACCGATCACAAAAACGACAATCTGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGCGGGAAGCCGTAGCAGAGTGCGCGTGTTATTATGCCGGATGCGGTGTaaa                                         <  2:2164361/149‑1 (MQ=255)
                                                                                                              ataATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                          <  2:2267803/150‑1 (MQ=255)
                                                                                                               taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                                        >  1:3937193/1‑150 (MQ=255)
                                                                                                                 aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt                                      >  1:2225329/1‑150 (MQ=255)
                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                     >  2:141671/1‑150 (MQ=255)
                                                                                                                    aaaTTATTTGTTTTTATATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAATGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                   >  1:1544778/1‑150 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                  <  1:4184807/150‑1 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                  >  2:717295/1‑150 (MQ=255)
                                                                                                                       ttattTGTTTTTAATTTTCGAGATAACGATCTCAAATACGACTATATGATAATTTTTCGAGGAGTGAAAGGCAAAAAATCGGCCTCCCGATTGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTTAACATACGTgg                                <  2:5823071/150‑1 (MQ=255)
                                                                                                                        tattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGc                               <  1:6479760/150‑1 (MQ=255)
                                                                                                                          ttttttttTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                             <  1:1293552/150‑1 (MQ=255)
                                                                                                                           ttttttttAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                           <  2:2795851/147‑1 (MQ=255)
                                                                                                                              tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                         >  2:3555194/1‑150 (MQ=255)
                                                                                                                              tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCt                         >  2:3254863/1‑150 (MQ=255)
                                                                                                                                tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                       >  2:4239999/1‑150 (MQ=255)
                                                                                                                                    aaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAg                   <  2:6043863/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACCAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                 <  2:6330552/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                 <  2:3055842/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                 <  2:3154650/150‑1 (MQ=255)
                                                                                                                                       atCGAGATAACGATCCCAAAAACGACCCTATGACAGTTATTCGTGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCATAGTGCGCGTGTTTTTATGCCGGATGCGGGGTCACCATCCGTGGCGGGGCGATGTGGGcg                >  2:4698599/1‑149 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa                >  1:1143154/1‑150 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa                >  1:4991218/1‑150 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa                >  2:4547062/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt               >  1:6014789/1‑150 (MQ=255)
                                                                                                                                           agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt            >  2:4200876/1‑150 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa          <  1:35542/150‑1 (MQ=255)
                                                                                                                                               aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa        <  1:2327640/150‑1 (MQ=255)
                                                                                                                                                aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc       >  2:5786807/1‑150 (MQ=255)
                                                                                                                                                 cGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACg      >  2:2465967/1‑150 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     >  2:5403781/1‑150 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     <  1:5275154/150‑1 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     >  1:6231262/1‑150 (MQ=255)
                                                                                                                                                    aTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCt    >  1:833864/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: