Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,914 A→G 5.3% intergenic (+521/+194) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,9140AG 5.3% 417.4 / 8.5 157intergenic (+521/+194)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (102/37);  new base G (5/3);  total (111/46)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

ATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304765‑2305063
                                                                                                                                                     |                                                                                                                                                     
 tAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTTGGTCGTATAAGGCGTTTACGCCGCATCCGAATTCTAACGCCCaa                                                                                                                                                      <  1:5549684/150‑1 (MQ=11)
   aGGCGGGTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:1485704/150‑1 (MQ=21)
    ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGcc                                                                                                                                                   >  2:1492372/1‑150 (MQ=21)
     gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                                                                                                                                  >  1:1144667/1‑150 (MQ=12)
        gtaaCGCCGCAGCCGACATCTAACGTCCGAGCCGGTTGGCTGATTGGTCGCTTGGTCGTCTTATGAGGCCTTCTGGGCTGTGATGTGGTGCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGtt                                                                                                                                               <  1:735383/147‑1 (MQ=2)
         ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                              <  1:3536393/150‑1 (MQ=33)
         ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                              >  1:3475410/1‑150 (MQ=33)
         ttACCCCGCACCCGACAACCACCGTCCGCGCCGTTGGCCGGAGGCGCCGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                              <  1:5838514/150‑1 (MQ=11)
          tACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             <  2:4373563/150‑1 (MQ=32)
           aCGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGcc                                                                                                                                            >  1:5771453/1‑150 (MQ=12)
             gccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGATAAGTCGGTAACGCCGCAGCCGACATCTAAAGCACAAGCAGGATGCCTg                                                                                                                                          >  2:1858892/1‑150 (MQ=11)
               cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGCTCAGGCGGTGACGCCGCCTCCGACATCTAACGCCCAAGCCGGTTGCCGGag                                                                                                                                        >  1:4549117/1‑149 (MQ=11)
                   tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGTCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    <  1:3397533/150‑1 (MQ=17)
                     cGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATCAGGCGGTTACGCCGCATCCGACCTCTACCGCCCAAGCCGGTTGCCTGAGGCGACg                                                                                                                                  >  2:1832115/1‑150 (MQ=255)
                      gACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                                 >  2:2242963/1‑150 (MQ=32)
                       aCATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCAAACGCCCCAGCCCGTTGCCTGATGCGACGcg                                                                                                                                >  1:5678021/1‑149 (MQ=12)
                         aTCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                              >  2:5133109/1‑150 (MQ=32)
                         aTCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                              >  2:5133086/1‑150 (MQ=32)
                          tCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCACCACCCCGTAGGTCGGATAAGGCGGGGACGCCGCAGCCGACATCTAACGCCCAAGCCGGGGGCCTGATGCGACGCGGgc                                                                                                                             >  2:4961976/1‑150 (MQ=11)
                          tCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCAAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                             >  1:4320092/1‑150 (MQ=12)
                          tCCAACGTCCGAGCCGGTTGCCAGAAGCGACGCGGGCGCGTCGTATCACGCCAACACCCCTGTGAAGTGCGCCACCCCCGAGGTCGGATAAGGCGGTTACGCCGCCACCCCCAGCAAACCCCCCACCCGGGAGCCAGAGGCGAcccacgc                                                                                                                             >  1:4155410/1‑144 (MQ=2)
                            cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           >  2:3117009/1‑150 (MQ=32)
                            cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           >  2:5462524/1‑150 (MQ=32)
                            cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAATACACCCATGAGTAGTGCTCATCCCCGTATGTCGGATAAGTACGAAGCGCGGCATCAGACATCTGACGCCCGAGCCGGATGCCTGATGCGACGCTGgctc                                                                                                                           >  2:3644295/1‑148 (MQ=2)
                                                                                                   aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGACGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCa                                                    >  1:3121201/1‑150 (MQ=2)
                                                                                                   aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGCAGTGCTCCACCCCGTAGGTTGGCTCAGGCGGTTACGCCGCAGCCa                                                    >  1:6191416/1‑150 (MQ=11)
                                                                                                     cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCAAc                                                  >  2:2335610/1‑150 (MQ=17)
                                                                                                       cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGATACGCCGCATCCAACAt                                                >  1:3897229/1‑150 (MQ=17)
                                                                                                           ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTaa                                            <  1:5460307/150‑1 (MQ=34)
                                                                                                           ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTaa                                            <  1:4343066/150‑1 (MQ=34)
                                                                                                           ggTCGGAGACGGCGGTTACGCCGCATCCGACATCCAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTaa                                            <  2:6091479/150‑1 (MQ=255)
                                                                                                            gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAAc                                           <  1:278700/150‑1 (MQ=34)
                                                                                                            gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAAc                                           <  2:6351980/150‑1 (MQ=34)
                                                                                                            gTCGGATAAGGCGGTTACGCCCCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAAc                                           <  1:278487/150‑1 (MQ=21)
                                                                                                             tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACg                                          >  2:5551046/1‑150 (MQ=34)
                                                                                                             tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATCCACCCCTGAGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGCTTACGCCGCATCCAACATCTAACg                                          >  2:4484998/1‑150 (MQ=11)
                                                                                                              cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGc                                         >  2:1373574/1‑150 (MQ=35)
                                                                                                              cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGc                                         <  1:894611/150‑1 (MQ=35)
                                                                                                              cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGc                                         >  2:4218857/1‑150 (MQ=35)
                                                                                                               ggATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                        >  1:1086435/1‑150 (MQ=35)
                                                                                                                gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccc                                       >  2:5037240/1‑150 (MQ=37)
                                                                                                                gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccc                                       >  2:113993/1‑150 (MQ=37)
                                                                                                                gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccc                                       >  2:4786874/1‑150 (MQ=37)
                                                                                                                gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccc                                       >  1:5187853/1‑150 (MQ=37)
                                                                                                                 aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCg                                      >  1:1531049/1‑150 (MQ=37)
                                                                                                                 aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCg                                      <  2:3283226/150‑1 (MQ=37)
                                                                                                                  tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGGGCTTTTTATCTGGCCTACACCGGTTTTAAGTGCTCAACCCCGTAGGTTGTATATGGCGGTTACGCCGCATCAAACATCTAACGCCCGa                                     <  1:4880346/150‑1 (MQ=11)
                                                                                                                  tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGa                                     <  2:4613429/150‑1 (MQ=17)
                                                                                                                     ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGcc                                  >  1:1459069/1‑150 (MQ=37)
                                                                                                                     ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGcc                                  >  2:3893917/1‑150 (MQ=37)
                                                                                                                      gctgTTCCGCAGCATCCTACATCTAACGCCCATGCCGTTTGCCTGTGGGGACTCTGGCGCTTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCTTAGGTTGTATATGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 <  1:338586/147‑1 (MQ=11)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTTGGATACGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 <  1:305066/150‑1 (MQ=255)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCg                                 >  2:305066/1‑150 (MQ=17)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCg                                 <  1:4975343/150‑1 (MQ=17)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 >  1:2563833/1‑150 (MQ=37)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 >  2:5675609/1‑150 (MQ=37)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 >  2:332926/1‑150 (MQ=37)
                                                                                                                       cGGTTACGCCACATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACAGCTGAGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCAACATCTAACTCCCGAGCCgg                                >  2:4239636/1‑150 (MQ=11)
                                                                                                                        ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGt                               >  2:4010266/1‑150 (MQ=37)
                                                                                                                        ggTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGt                               >  2:4010319/1‑150 (MQ=37)
                                                                                                                         gTTACGCCGCAACCTACATCCACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCACCCCTTTGGTTTGTTAAGGCGGTTAGGCCGCATCCAACATCTAACGCCCGAGCCGGtt                              <  1:3749409/150‑1 (MQ=11)
                                                                                                                          ttACGCCGCATCCTACTTCTACCGCCCCCGCGGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTg                             <  1:3791437/150‑1 (MQ=11)
                                                                                                                           tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc                            <  2:4320092/150‑1 (MQ=37)
                                                                                                                           tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc                            >  2:6358115/1‑150 (MQ=37)
                                                                                                                             cgccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                          >  2:2648011/1‑150 (MQ=37)
                                                                                                                               ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGa                        >  1:925663/1‑150 (MQ=37)
                                                                                                                                cgcAACCTACATCCAACGACACCGCCGTTTGCCTTATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGAAGTGATCCACCCCGTAGGTTGGATATGGCGGTTCCGCCTCATACAAAATATAACGCCCGAGCCGGTTGCCTGAt                       <  1:2946599/150‑1 (MQ=11)
                                                                                                                                 gcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATg                      >  1:6589070/1‑150 (MQ=37)
                                                                                                                                    tCCGACATCTAACGCACAATACGTTTGCCTGATGCGACTCTTGCTCGTCTTATAAGGCATACACCGCTGTGAAGTGCTCCACCCCCTATGTTGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCGAGCCGGTTGCCTGATGCGa                   <  2:2956940/150‑1 (MQ=11)
                                                                                                                                      cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                 <  2:1371128/134‑1 (MQ=37)
                                                                                                                                       gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACCTCTAACGCCCGAGCCGGTTGCCTGATGCGACGc                >  1:4031901/1‑150 (MQ=37)
                                                                                                                                       gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGc                >  1:4031989/1‑150 (MQ=37)
                                                                                                                                       gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGc                >  1:6564824/1‑150 (MQ=37)
                                                                                                                                        aCATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCt               >  1:651676/1‑150 (MQ=37)
                                                                                                                                        aCATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCt               >  2:411265/1‑150 (MQ=37)
                                                                                                                                          aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCt                                              >  2:4889859/1‑117 (MQ=33)
                                                                                                                                          aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCt                                              <  1:4889859/117‑1 (MQ=33)
                                                                                                                                           tccaaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAAc                                                  <  2:5957995/109‑1 (MQ=0)
                                                                                                                                           tccaaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAAc                                                  >  1:5957995/4‑112 (MQ=0)
                                                                                                                                            cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGc                                         <  2:3618285/120‑1 (MQ=37)
                                                                                                                                            cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCCAACGc                                         >  1:3618285/1‑120 (MQ=11)
                                                                                                                                             tAACGCCCTAGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  1:2330829/150‑1 (MQ=255)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          >  2:550235/1‑150 (MQ=37)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  2:2152734/150‑1 (MQ=37)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCGGgcgc          >  1:5847274/1‑150 (MQ=37)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGAAGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          >  1:6087930/1‑150 (MQ=37)
                                                                                                                                              aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGCTAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  2:1776748/1‑150 (MQ=21)
                                                                                                                                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGTCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGAGACGCTGGcgcg         >  1:20157/1‑150 (MQ=255)
                                                                                                                                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  1:1555925/1‑150 (MQ=37)
                                                                                                                                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  1:5303718/1‑150 (MQ=37)
                                                                                                                                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt        >  1:4849549/1‑150 (MQ=37)
                                                                                                                                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt        <  1:5787081/150‑1 (MQ=37)
                                                                                                                                                 gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt      <  2:2563833/150‑1 (MQ=37)
                                                                                                                                                 gCCCAAGCCCGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt      <  1:5381433/150‑1 (MQ=255)
                                                                                                                                                   ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa    <  2:552189/150‑1 (MQ=37)
                                                                                                                                                   ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa    <  2:3211543/150‑1 (MQ=37)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   >  1:4822558/1‑150 (MQ=37)
                                                                                                                                                     aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  2:4524346/1‑150 (MQ=37)
                                                                                                                                                     aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  1:839552/1‑150 (MQ=37)
                                                                                                                                                     aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACAACTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc  >  1:6138481/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304765‑2305063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: