Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 9.4% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT 9.4% 660.6 / 5.6 228R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (89/109);  new base T (20/4);  total (115/113)
Fisher's exact test for biased strand distribution p-value = 3.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580
                                                                                                                                                    |                                                                                                                                                     
 taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATaa                                                                                                                                                     >  1:534530/1‑150 (MQ=255)
 taTCTCTTTTTCGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGATATTTAAGCAAGCATTTATTTTGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTataaaaaaaataaaaaaa                                                                                                                                                     >  1:1131399/1‑150 (MQ=255)
   tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAATaaaa                                                                                                                                                   >  2:5697272/1‑149 (MQ=255)
   tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAATTaaaaaaaaa                                                                                                                                                   >  1:598371/1‑149 (MQ=255)
    ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTAGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                                  <  2:6099250/150‑1 (MQ=255)
     tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAATTAaaataaataa                                                                                                                                                 >  1:1362914/1‑148 (MQ=255)
     tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                 >  2:6583753/1‑150 (MQ=255)
      ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATaaaaaaa                                                                                                                                                >  1:4527145/1‑146 (MQ=255)
       tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAtaaaataaataaaataaaaaaaa                                                                                                                                               >  2:1782252/1‑145 (MQ=255)
                 atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  2:2000071/150‑1 (MQ=255)
                 atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  2:722602/150‑1 (MQ=255)
                  tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  2:3287032/150‑1 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                  >  2:3815619/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGCTGt                                                                                                                                  >  2:2997496/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGAAAATAGCGAttt                                                                                                                                  >  1:5923714/1‑148 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAAATCAAAGAAAACAAGACAATAGCGATGt                                                                                                                                  >  2:6508238/1‑150 (MQ=255)
                     cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAAAATCGATGTa                                                                                                                                 >  1:983975/1‑150 (MQ=255)
                      aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGAAAATAGCGATGTaa                                                                                                                                >  1:2702440/1‑150 (MQ=255)
                      aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATAAAGACAAAAGCGATTTaa                                                                                                                                >  2:5822933/1‑150 (MQ=255)
                        ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  1:3510214/150‑1 (MQ=255)
                        ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  2:3410847/150‑1 (MQ=255)
                         tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                             <  2:1624487/150‑1 (MQ=255)
                         tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAATAAATAAAGAAAATAGCGATGTaaaac                                                                                                                             >  2:5430116/1‑148 (MQ=255)
                               tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATAAAATAATTCAAAAAAATAAACAAAAAAGAGATGTAAAGAACTAAg                                                                                                                       >  1:3029093/1‑150 (MQ=255)
                               tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                       <  2:2475493/150‑1 (MQ=255)
                               tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAAACAAAAGACAAGTAAAGAACTAAg                                                                                                                       >  1:1503719/1‑150 (MQ=255)
                                 tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAACGATGTAAAGCACAAAGtt                                                                                                                     >  1:4638139/1‑150 (MQ=255)
                                   aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAATATCGATGTAAATCACTAAGTTTa                                                                                                                   >  2:6384961/1‑150 (MQ=255)
                                    tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                  <  2:2921438/150‑1 (MQ=255)
                                     ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                 <  2:3251291/150‑1 (MQ=255)
                                      tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAATAAAAAAGCGATGTAAAGCACTAATTTTAAAt                                                                                                                >  1:2105262/1‑150 (MQ=255)
                                            ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTGCAAGAAATCAAGACAAAAGCGAGGTAAAGCACGAAGTTTAAATTgcgcg                                                                                                          >  2:2727534/1‑150 (MQ=255)
                                             gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAAAAAAAAGCGATGTAAAGCACGAAGTTTAAATTtagcgc                                                                                                         >  1:4799007/1‑150 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGAAAAAAGCGATTTAAAGCACAAAGTTTAAATTTAGCGCCAATccag                                                                                                 >  2:5226398/1‑147 (MQ=255)
                                                      agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                <  2:2762810/150‑1 (MQ=255)
                                                      agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAAAAAGACAATATCGATTTAAAACAAAAAGTTTAAATTTCGCGCCAAAAATgg                                                                                                >  2:2577270/1‑150 (MQ=255)
                                                         gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  2:1374361/150‑1 (MQ=255)
                                                         gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGATTATAGATAACAGTCGTTTTTTTAATTTATAATATAATTCAAAGAAATCTAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  2:3152826/150‑1 (MQ=255)
                                                         gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  1:39268/150‑1 (MQ=255)
                                                         gaTTAAAGCAAGCATTTATTTGCAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAACTTGCGCGCCAATCATGgcgc                                                                                             <  2:6277952/150‑1 (MQ=255)
                                                          aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGATGTAAATCACAAAGTTTAAATTTCGCGCCAAACaattcgcg                                                                                            >  2:6219706/1‑143 (MQ=255)
                                                            ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAAAAATTAAAAAAAAAAAAGAAAAAATCGATGTAAAGCACGAATTTTAAATTTAGGGCCCAACAGGGCGCGca                                                                                          >  1:2137774/1‑150 (MQ=255)
                                                            ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAAAAAATAAAGAAAATATAGAGTTAAAAGACTAAGTTTAAATTTAGCGCCCAACAATgcgcgcc                                                                                          >  1:4872037/1‑149 (MQ=255)
                                                            ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                          <  2:5318773/150‑1 (MQ=255)
                                                                gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                      <  2:4919517/150‑1 (MQ=255)
                                                                gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAACACAAAACCGCTGTAAAACCCAAACGGTAAATTGCGCCCCAATCAAGGCCCCcaccac                                                                                      >  2:1125064/1‑147 (MQ=255)
                                                                  aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                                    <  2:1933405/150‑1 (MQ=255)
                                                                  aagaaTTTATTTGGAATGTTTTTTTCGTTCGACCTTTGATTATACATAACATTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCATGATTGGCGCGcaatttaa                                                                                    <  1:3942175/150‑7 (MQ=255)
                                                                   agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                   <  2:3027727/150‑1 (MQ=255)
                                                                       tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGGCGAGCACAAGCTataat                                                                               >  2:956105/1‑150 (MQ=255)
                                                                       tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAATCAATAAGTTTAAATTGCGCGCCAATACTGGCGCGCACAAGCTataat                                                                               >  2:6617677/1‑150 (MQ=255)
                                                                       tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAAATGAAAGAAATAAAGACAAAAGCGCAGTAAAACAAAAAATTTAAATTTAGGGCCCAACAGGGCGCGCCCAAGCGataaa                                                                               >  1:3372836/1‑149 (MQ=255)
                                                                          atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAcc                                                                            <  2:2843252/150‑1 (MQ=255)
                                                                            ttttattGTTTTTTTCTTTCGACGTTTGTTTATAAATAACTGTCTTTTTTTTAATTTATAAATTAATTAAAATAAATAAAGAAATTAGCGATTTAAAGAACTATTTTTAAATTGCGCGCAAATATTGGGGCGCACAAGCTATAATACCaa                                                                          <  1:1026009/144‑1 (MQ=255)
                                                                             tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                         <  2:2068699/150‑1 (MQ=255)
                                                                               gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATTGCGCGCACAAGCTATAATACCAACCt                                                                       >  2:2151869/1‑150 (MQ=255)
                                                                                       ttttCGTTCGACCTTTGCTTATACATAACTTTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  1:4155592/150‑1 (MQ=255)
                                                                                       ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTACAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  1:4650769/150‑1 (MQ=255)
                                                                                       ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  2:3959262/150‑1 (MQ=255)
                                                                                        tttCGTTCTACCTTTGATTATACATAAAAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCTTTTTAAAGAACTATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                              <  1:1714410/150‑1 (MQ=255)
                                                                                        tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                              <  1:2051486/150‑1 (MQ=255)
                                                                                           cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                           <  2:6020620/150‑1 (MQ=255)
                                                                                                          tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                            <  2:4645955/150‑1 (MQ=255)
                                                                                                          tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGTCAATATCGTTGTAAATCACAAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTATCCTCCTCTGAGGGTGACtat                                            >  2:5781734/1‑150 (MQ=255)
                                                                                                            taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  2:1331053/150‑1 (MQ=255)
                                                                                                               aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                       <  1:3802912/150‑1 (MQ=255)
                                                                                                                tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGAGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                      <  2:4830823/150‑1 (MQ=255)
                                                                                                                 aaCAGTCGTTTTTTTAATTTAAAAAAAAATTCAAAAAAAACAAGACAAAATCGATGTAAAGCACAAAGTTTAAATTTCGCCCCAAACAAGGCGCGCACAACCAATAAAAACAAACAAAATTATCCTCCGCAAAGACTGGATAAATCTCCt                                     >  2:845803/1‑150 (MQ=255)
                                                                                                                     gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                 <  2:5579118/150‑1 (MQ=255)
                                                                                                                      tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                >  2:1450622/1‑150 (MQ=255)
                                                                                                                         tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:6401027/1‑150 (MQ=255)
                                                                                                                           tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                           >  1:4727644/1‑150 (MQ=255)
                                                                                                                           tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                           >  2:6354006/1‑150 (MQ=255)
                                                                                                                            ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATCTCTCCTGATAGAATTGc                          <  2:6060611/150‑1 (MQ=255)
                                                                                                                            ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                          <  1:35351/150‑1 (MQ=255)
                                                                                                                             tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  1:790666/150‑1 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  1:2593142/1‑150 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  1:4127943/1‑150 (MQ=255)
                                                                                                                                  tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                    >  1:1845652/1‑150 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTAGTGAc                 >  2:6598638/1‑150 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 <  2:6482021/150‑1 (MQ=255)
                                                                                                                                     ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGAACAAGCAATAATACCAACCTAATTTCACCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 >  1:4350615/1‑150 (MQ=255)
                                                                                                                                        aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              <  1:2525459/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTCGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACtctc             >  2:2477306/1‑150 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  2:598332/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  2:598382/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  1:575054/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             <  2:3937075/150‑1 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             >  2:4349955/1‑150 (MQ=255)
                                                                                                                                         aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             >  1:387945/1‑150 (MQ=255)
                                                                                                                                                cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      <  1:543359/150‑1 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:4625543/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  2:4524036/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     <  1:6219706/150‑1 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     <  1:6444888/150‑1 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACTATCAAATGCCCt    >  1:277229/1‑150 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:3614871/1‑150 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:5094498/150‑1 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:5645462/150‑1 (MQ=255)
                                                                                                                                                   aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg   >  2:738205/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: