Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,328 A→C 6.7% intergenic (‑300/+130) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,3280AC 6.7% 1188.5 / 11.1 434intergenic (‑300/+130)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (263/121);  new base C (2/39);  total (266/168)
Fisher's exact test for biased strand distribution p-value = 4.32e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 447 total aligned reads displayed.

GTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAC  >  NC_000913/3392180‑3392477
                                                                                                                                                     |                                                                                                                                                      
gTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑g                                                                                                                                                      >  2:3494062/1‑150 (MQ=255)
   aaCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATACGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGGATCCGACCTACGGGTATGTTCCG‑GGTGTAGGCCTGATCAGACGCACA‑GCgg                                                                                                                                                   >  2:4616266/1‑149 (MQ=14)
    aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTc                                                                                                                                                  >  2:4692218/1‑150 (MQ=255)
    aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTACGCCTGATAAGACGCACA‑GCGTc                                                                                                                                                  >  2:2571009/1‑150 (MQ=255)
    aCGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTATGTTCAG‑TATGTAGGCCTGATAAGACGcacatagtc                                                                                                                                                   >  2:2448328/1‑145 (MQ=255)
     ccccTTATCCGACCTACGGTTCTGTTCCGTTTGGTGGCCTTATAAGACGCACTGGGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCg                                                                                                                                                 <  2:1124037/148‑1 (MQ=14)
     cGCCTTAACCGACCTACGTTTATGTTCCGTTTGTCGGCCTTATAAGACGCCCAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCg                                                                                                                                                 <  1:2039003/150‑1 (MQ=14)
      gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTATGTTCCG‑TATCTATGCCTTATAAGAAGCACA‑gacaaga                                                                                                                                                >  1:1451597/1‑144 (MQ=14)
         ttATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATc                                                                                                                                             >  2:6228661/1‑150 (MQ=255)
         ttATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATc                                                                                                                                             >  1:4272226/1‑150 (MQ=255)
         ttATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCG‑TTTGTAGGCCTGTTAAGACGCACA‑GCGTCGCATc                                                                                                                                             >  2:5032569/1‑150 (MQ=255)
         ttATCCGACCTACGGTTATGTGCCGTTTGTAGGCCTGATAAGACGCACAGCGGCGCATCAGGCCACGGCTGCCGGAGGCGGCGTAAACGCCGTAGCCGACCTACGGTTAGGTTCCG‑TGTGTAGGCCTGATCAGACGCACC‑GCGGCGCATc                                                                                                                                             >  2:769826/1‑150 (MQ=14)
          tttCCGACATTGGGTTGTGTTCCGTTTTTTGCCCTTAAAAGACGCCCAGCGTCGCATATGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCa                                                                                                                                            <  1:49103/148‑1 (MQ=11)
          tATCCGACCTCCGTTTAGGTTCCGTTTGTAGCCCTGATAAGACGCACAGCGTCGCATCCGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCa                                                                                                                                            <  1:925272/150‑1 (MQ=14)
          tATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGCCGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCa                                                                                                                                            <  2:3261439/150‑1 (MQ=255)
           aTCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAg                                                                                                                                           >  1:2355934/1‑150 (MQ=40)
            tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAgg                                                                                                                                          >  1:5550243/1‑150 (MQ=40)
                       gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGcc                                                                                                                               >  1:6195756/1‑150 (MQ=35)
                       gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGcc                                                                                                                               >  2:6053867/1‑150 (MQ=35)
                       gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGcc                                                                                                                               >  1:551433/1‑150 (MQ=25)
                             ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                                                                         >  2:1784631/1‑150 (MQ=35)
                             ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAAGGCTGCCGGAGGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCG‑TGTGGAGGCCGGATACGACGCACT‑GCGTCGCATCAGTCCACGGCTGCCGGATgc                                                                                                                         >  2:4126841/1‑150 (MQ=11)
                             ttCCGTTTGTAGGCCGGATACGACGCACAGCGTCGCATCAGGAAACGGCTGCCGTATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCTGGCAACGGCTGCCGGATgc                                                                                                                         <  2:430761/150‑1 (MQ=11)
                                        ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGc                                                                                                              <  2:4832063/150‑1 (MQ=34)
                                         gCCCGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGCGTAAACGCCTTACCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc                                                                                                             <  2:3809711/150‑1 (MQ=255)
                                              ttAAGACGCTCAGCGTCGCCTCTGTCAACGGGTGCCGGATGCGGCGTCACCGCCTTAGCCGCCCTACGTTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATc                                                                                                        <  2:3159110/149‑1 (MQ=11)
                                               tAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTGTTCCG‑GGTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATcc                                                                                                       >  2:26444/1‑150 (MQ=255)
                                               tAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATcc                                                                                                       >  1:1909439/1‑150 (MQ=37)
                                                 acacccacaGCCTCGCAACAGCAAACGGCTGCCGGATGCGGCGTAAACGCATTATCCGACATACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATAAGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGa                                                                                                     <  2:1932686/145‑1 (MQ=11)
                                                   aCGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGAcc                                                                                                   >  2:5435449/1‑150 (MQ=37)
                                                    cGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGCAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCt                                                                                                  >  2:219746/1‑150 (MQ=37)
                                                        caGCTTCGCATCTGGAAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACgg                                                                                              <  2:6260018/150‑1 (MQ=255)
                                                            gTCGAATCTGGAAACGGCTGCCGGATGCGGCTTAAACGCCTTATCCTACCTAGGGTTTTTTTCCT‑TTTGTCGGCCTGATAAGACGCACT‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTAt                                                                                          <  1:4312108/150‑1 (MQ=11)
                                                              cGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGt                                                                                        >  1:5204141/1‑150 (MQ=37)
                                                               gCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGtt                                                                                       >  1:6013261/1‑150 (MQ=37)
                                                               gCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGtt                                                                                       >  1:3380887/1‑150 (MQ=37)
                                                                 aTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCt                                                                                     >  1:4986463/1‑150 (MQ=38)
                                                                      gCATCGGCTGCCGTATGCGGCTTAAACGCGTTATCCTCCCTAGGGTTTTGTTCCG‑TTTGTAGGCCTGATAAGACTCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTc                                                                                <  1:3986033/150‑1 (MQ=14)
                                                                      gCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTAGGTTCCG‑TTTGTAGGCCTGATAAGACACACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTc                                                                                <  1:857654/150‑1 (MQ=255)
                                                                       cAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCg                                                                               >  1:6503373/1‑150 (MQ=255)
                                                                       cAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCg                                                                               >  2:1292234/1‑150 (MQ=255)
                                                                            gcggCCGTATGCGGCGTAAACGCCTTATCCGACCTACGTTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGc                                                                          <  2:3405476/147‑1 (MQ=255)
                                                                                ccGGTTGCGGCGTAAACGCCTTTTCCTACCTACGGTTTTTTTCCG‑TTTGTAGGCCTGATAAGACGCACT‑GCGTCGCATCAGGCAAGGGCTGCCGTATGCGGCGTGAACGCCCTATCCGACCTACGTTTTTGTTCTGGCTCGTTGGCCttt                                                                      <  2:4384216/150‑1 (MQ=17)
                                                                                ccGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTTGGCCTGATAAGACGCACC‑GCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCttt                                                                      <  2:5391846/150‑1 (MQ=255)
                                                                                ccGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCttt                                                                      >  2:1519284/1‑150 (MQ=255)
                                                                                ccGGACGCGGCGTAAACGCCTTAACCGCCCTACGGTTTTGTTCCG‑TTTGTAGGCCTGATAAGTCGCCCA‑GCGGCGCACCAGGCATCTGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCttt                                                                      <  2:6465657/150‑1 (MQ=17)
                                                                                 cggtggcggcgTAACCGCCTTACCCGACCTCCGTTTATGTTCCG‑TTTGTCGGCCTGATAAGACGCACC‑GGGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  2:2826143/145‑1 (MQ=17)
                                                                                 cGGAGGCGGCTTAAACGCCTTATCCGACCTAGGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACCCACA‑GCGTCGAATCAGCCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  1:5936746/150‑1 (MQ=17)
                                                                                 cGGACGCGGCGAAAACGCCTTTCCCGACCGCCGGTTCTGTTCGG‑TTTGTAGGCATGATAAGACGCACA‑GCGTCGCATCCGGCAACGGGTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTg                                                                     <  2:235474/150‑1 (MQ=17)
                                                                                  ggATGCGGCGTAAACGCCTTATCCGACCTAGGGTTTTGTTCGG‑TTTGTCGGCCTGATAAGACGCACA‑GCGTCGCATCAGGACACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                                    <  1:5645988/150‑1 (MQ=17)
                                                                                  ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                                    <  2:3567477/150‑1 (MQ=255)
                                                                                  ggAGGCGGCTTAAACGCATTAACCGACATCCGTTTATGGGCGT‑TTTGTTGGCCTGAAATGACG‑ACATGCGTCTCATCTGCCTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTgg                                                                    <  1:170926/150‑1 (MQ=12)
                                                                                     tGCGGCGTAAACGCCTTATCCGACAT‑CGGTTTTTTTTCGTTTTGTCGGCCTTATAAGACGCACC‑GCGTCGCAACAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCaa                                                                 <  1:1219087/150‑1 (MQ=17)
                                                                                         gcgTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGAt                                                             >  1:5677321/1‑150 (MQ=255)
                                                                                             aaaCGCCTTATCCTACCTACGGTTTTGTTCGT‑TTTGTTGGCCTGATAAGACGCACC‑GCGTCGCAACTGGAAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATc                                                         <  1:6389543/150‑1 (MQ=18)
                                                                                             aaaCGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACAGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATc                                                         <  1:2740489/150‑1 (MQ=255)
                                                                                                  ccTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATg                                                    >  1:1023286/1‑150 (MQ=255)
                                                                                                   cTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCACCGATTATCCTATga                                                   >  2:4804692/1‑150 (MQ=255)
                                                                                                   cTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATga                                                   >  2:3654160/1‑150 (MQ=255)
                                                                                                   cTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCATTGGCAACGATTATCCTATga                                                   >  2:5710254/1‑150 (MQ=255)
                                                                                                    ttATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGTGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATgag                                                  >  1:785588/1‑150 (MQ=255)
                                                                                                    ttATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATgag                                                  >  2:4494807/1‑150 (MQ=255)
                                                                                                    ttATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCATATgag                                                  >  1:626539/1‑150 (MQ=255)
                                                                                                    ttATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCATATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCAATgag                                                  >  1:2490012/1‑150 (MQ=255)
                                                                                                        ccGACCTCGGGTTTTGTCCGG‑TTTTTAGTCCTGTTAAGACGCCAT‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                              <  1:3149789/150‑1 (MQ=21)
                                                                                                        ccGAACTACGGTTATGTTCCG‑TTTGTAGGCCTGTTAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTg                                              <  1:2196322/150‑1 (MQ=255)
                                                                                                         cgcccTACGGTTATTTTCCG‑TTTGTAGGCCTGATAAGACGCCCC‑GCGTCGCTTCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgg                                             <  1:5159613/147‑1 (MQ=21)
                                                                                                         cGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgg                                             >  2:1540962/1‑150 (MQ=255)
                                                                                                          gACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTggg                                            >  1:4565596/1‑150 (MQ=255)
                                                                                                          gACATACGGTTTTTTTCCG‑TTTGTAGGCCTGATAAGCCGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTggg                                            <  2:2958154/150‑1 (MQ=255)
                                                                                                           aCCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTgggg                                           >  1:2632178/1‑150 (MQ=255)
                                                                                                            ccaaCGGTTATTTACCG‑TTTGTCGGCCTGATAAGACGCACA‑CCGTCGAAACAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGc                                          <  1:3498645/147‑1 (MQ=21)
                                                                                                                cGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAAtt                                      >  1:3786857/1‑150 (MQ=255)
                                                                                                                cGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAAtt                                      >  1:6055818/1‑150 (MQ=255)
                                                                                                                  gTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAc                                    >  1:1245008/1‑150 (MQ=255)
                                                                                                                   ttATGTCCCG‑TTTGTTCTCCTGTGAAGCCGCACA‑GCGTCGGATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcg                                   <  2:3995488/150‑1 (MQ=25)
                                                                                                                    tttGTTCCG‑TTTGTCGGCCTTATAAGACGCACA‑GGGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcgc                                  <  2:557854/148‑1 (MQ=255)
                                                                                                                    tATGTTCCG‑TTTGTTGGCCTGATAAGCCGCACC‑GCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcgc                                  <  1:1855945/150‑1 (MQ=255)
                                                                                                                    tATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcgc                                  <  2:717043/150‑1 (MQ=255)
                                                                                                                     aTGTTCGG‑TTTGTAGGCCTGATAAGACGCACC‑GCGTGGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTAcgcg                                 <  1:4132042/150‑1 (MQ=255)
                                                                                                                        ttCCG‑TTTGTAGGCCTGCTAAGACGCACA‑GCGGCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGccc                              <  1:5999575/150‑1 (MQ=255)
                                                                                                                        ttCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGccc                              >  1:2144523/1‑150 (MQ=255)
                                                                                                                        tccgg‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTTAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGccc                              <  1:6486539/146‑1 (MQ=255)
                                                                                                                         tCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCt                             >  1:5820218/1‑150 (MQ=255)
                                                                                                                            g‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGt                          <  1:4757905/150‑1 (MQ=255)
                                                                                                                                 gTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCaaa                      <  1:5394180/150‑1 (MQ=255)
                                                                                                                                     gCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCAt                  >  2:5235891/1‑150 (MQ=255)
                                                                                                                                      ccTTATAAGCCGCACC‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCAtt                 <  1:1750619/150‑1 (MQ=255)
                                                                                                                                         gATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGGTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgcg              >  1:2891647/1‑150 (MQ=255)
                                                                                                                                          ctAAGCCGCACC‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTgcgc             <  2:4165235/149‑1 (MQ=255)
                                                                                                                                              gACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCtttt         >  2:5776155/1‑150 (MQ=255)
                                                                                                                                              gACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCtttt         >  1:4637273/1‑150 (MQ=255)
                                                                                                                                               aCGCACC‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCttttt        <  1:3040585/150‑1 (MQ=255)
                                                                                                                                                 gCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCt      >  2:2056041/1‑150 (MQ=255)
                                                                                                                                                 gCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCt      <  1:1642116/150‑1 (MQ=255)
                                                                                                                                                 gCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCGTTGGCAACGATTCTCCTATGAGAGTGGGGCACTTACGCGCCCGCGGCACATCATGGCGCTTTTTCt      >  2:1258382/1‑150 (MQ=25)
                                                                                                                                                  caca‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCACATCATTGCGCTTTTTCtt     >  1:947884/1‑150 (MQ=255)
                                                                                                                                                   aca‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCttt    <  2:4210462/150‑1 (MQ=255)
                                                                                                                                                   aca‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCttt    <  2:5635802/150‑1 (MQ=255)
                                                                                                                                                     a‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAc  >  1:4506088/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCG‑TTTGTAGGCCTGATAAGACGCACA‑GCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCTTTGGCAACGATTATCCTATGAGATTGGGGCAATTACGCGCCCTCGTCAAATCATTGCGCTTTTTCTTTAC  >  NC_000913/3392180‑3392477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑