Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 5.5% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 5.5% 682.9 / 3.0 273intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (102/149);  new base G (7/15);  total (109/164)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

CAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504708‑3505004
                                                                                                                                                   |                                                                                                                                                     
cAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTc                                                                                                                                                     <  2:24924/150‑1 (MQ=255)
 aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCATAACTATTGATAATAAAAGACAGTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  2:6245402/1‑150 (MQ=255)
   aCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcccc                                                                                                                                                  >  2:3100794/1‑150 (MQ=255)
    ccGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                                 >  1:4830577/1‑150 (MQ=255)
       gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  1:6197588/150‑1 (MQ=255)
       gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  1:5155934/150‑1 (MQ=255)
          cACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAcccg                                                                                                                                           >  2:4713127/1‑149 (MQ=255)
           aCGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACccccca                                                                                                                                          >  2:5175878/1‑148 (MQ=255)
           aCGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                          >  1:3565948/1‑150 (MQ=255)
            cGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTaa                                                                                                                                         <  2:2907790/150‑1 (MQ=255)
            cGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTaa                                                                                                                                         <  1:819522/150‑1 (MQ=255)
            cGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAACTTTACATCCCGTACGTTCCCCTCACCCTaa                                                                                                                                         >  1:2225578/1‑150 (MQ=255)
              tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  1:3606074/150‑1 (MQ=255)
              tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  1:5487881/150‑1 (MQ=255)
              tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  1:3408332/150‑1 (MQ=255)
              tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  1:2927684/150‑1 (MQ=255)
                tcCGAACTCTTCTGCCGCGCCAATCGCATCTCCTTAACTATTGATAATAAAAGAGATTATCCGTAGGCCGGATATGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                     >  2:1522000/1‑150 (MQ=255)
                tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAccca                                                                                                                                     >  1:932391/1‑149 (MQ=255)
                 ccGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctc                                                                                                                                    <  1:1523457/150‑1 (MQ=255)
                  cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCCAAcccaca                                                                                                                                   >  1:4748976/1‑147 (MQ=255)
                   gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  2:5288277/150‑1 (MQ=255)
                   gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCgctc                                                                                                                                  >  1:4473165/1‑150 (MQ=255)
                       tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                              >  2:6465445/1‑150 (MQ=255)
                       tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCa                                                                                                                              >  2:6512582/1‑150 (MQ=255)
                       tcttctGCCGCGCCAATCGCATCACCTTAACTAGTGATAATAAAAGACTGTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGTTGCTGGCGCGTTTAATTTTACATCCCTTACGTTCCCATCACCCTAACCCTCTcccaa                                                                                                                              <  2:3035497/150‑3 (MQ=38)
                        cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                                                                             <  1:6611239/150‑1 (MQ=255)
                        cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaa                                                                                                                             >  2:2187349/1‑150 (MQ=255)
                         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCCAACCCACTcccccac                                                                                                                            >  1:1973484/1‑147 (MQ=255)
                         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACAACCCGTACGTTCCCCTCACCCTAACCCACTCCCCaca                                                                                                                            >  2:5520922/1‑148 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  2:4111876/150‑1 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  2:1651560/150‑1 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAg                                                                                                                           >  2:4001096/1‑150 (MQ=255)
                            tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                         <  1:2286316/150‑1 (MQ=255)
                            tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                         <  1:3931722/150‑1 (MQ=255)
                              ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                       >  2:6067675/1‑150 (MQ=255)
                                gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:5271034/150‑1 (MQ=255)
                                gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:371164/150‑1 (MQ=255)
                                gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTACCCAAAGGGGCgg                                                                                                                     >  1:1089890/1‑149 (MQ=255)
                                  gcCAATCGCATCACCTTCACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  2:3824757/150‑1 (MQ=39)
                                  gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  1:3321359/150‑1 (MQ=39)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  2:709786/150‑1 (MQ=255)
                                      aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAAACCACAACCCAAAGGGGCGAGGGGAc                                                                                                               >  1:6460781/1‑150 (MQ=38)
                                          cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAACCCACACCCCAAAGGGGCGAGGGGGCCGtt                                                                                                           >  1:4579448/1‑150 (MQ=39)
                                           gtCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCACTACCCAAAGGGGCGAGGGGCCCGGTc                                                                                                          >  1:6072766/2‑150 (MQ=38)
                                           aTCACCTGAACTATTGATAATAAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTCTTTTACATCCCGTTCGTTCCCCGCACCCAAACCCGCTCCGCAAAGGGGCGCGGGGGCCGGTc                                                                                                          >  2:6403441/1‑150 (MQ=37)
                                            tCACCTTACCTAGTGATAATACAAGACAGTCCCCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGCATTTTACATCCCGTACGTTCCCCTCACCCTCCCCCTCTCCCCCCAGGGGCGAGGGGACCGTTCa                                                                                                         <  2:1125527/150‑1 (MQ=37)
                                            tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTTCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:2035409/150‑1 (MQ=255)
                                             cACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTGCAg                                                                                                        >  2:432847/1‑150 (MQ=255)
                                               tcTTTACTTTTGATAATAAAAGACATTATCCGTAGGCCGGATTTGATGCGCCTGCATCGCATCAGGCGATGCTGGCGCGTTGAATTTTAGATCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCAATGGGGCGAGGGGACCGTTCTGtt                                                                                                      <  2:2046326/149‑2 (MQ=37)
                                                cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAAACCGCTCCCCCAAGGGGGGGGGGGGCCGTTCAGTaa                                                                                                     >  1:1464695/1‑149 (MQ=38)
                                                cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCACACCCAAACCCACAACCCCAAGGGGCGAGGGGGACGGTCAGTAc                                                                                                     >  1:128061/1‑150 (MQ=37)
                                                  tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACgg                                                                                                   <  2:590125/150‑1 (MQ=255)
                                                     cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAt                                                                                                <  1:2597103/150‑1 (MQ=255)
                                                       aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAAttt                                                                                              >  2:510056/1‑150 (MQ=255)
                                                       aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCCCATCCGGCGATGCAGGCGCGAAGAATTTTACAACCAGTACGGTCACCACACCCAAACCCACAACCCAAAAGGGCGGGCGGACCCGTCAGTACGGaaaat                                                                                              >  1:4954078/1‑147 (MQ=21)
                                                         tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                            <  1:1556015/150‑1 (MQ=255)
                                                          gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                           <  2:2847091/150‑1 (MQ=255)
                                                          gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCTCACCCCCAACGGGCGAGGGGACCCGTCAGTACCGAATTTAGc                                                                                           >  2:3946888/1‑150 (MQ=38)
                                                            tactaaAAGACTTTAACCGTCGGCCGGCTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTGGAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTCCCCCACGGGGCGATGGGACCGAGCAGTACGGAATTTAGCtg                                                                                         >  2:3770938/4‑149 (MQ=255)
                                                            taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                         <  2:3580240/150‑1 (MQ=255)
                                                                aaaaGACATTAACCGTAGGCCGGATTAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTGCCCAAAGGGGCGAGGGGACCGTTGAGTACGGAATTTAGCTTGACg                                                                                     <  2:6083961/150‑1 (MQ=255)
                                                                  aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                   <  1:1199457/150‑1 (MQ=255)
                                                                   aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCAACCCAACCCAAAACCCAAACGGGCGCGGGGACCGGTCACTACGGAATTTAGGGTGACgggg                                                                                  >  2:2384745/1‑148 (MQ=25)
                                                                   aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:1330202/150‑1 (MQ=255)
                                                                   aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  2:3565948/150‑1 (MQ=255)
                                                                    gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGTTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACCGAATTTAGCTTTACGGTGc                                                                                 >  1:4894576/1‑150 (MQ=39)
                                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCGCACCCAAAACCACACCCCCAACGGGCGTGGGGGCCCTTCATTAAGGAATTTATCGTGAAGGTGCTAt                                                                              >  1:632640/1‑150 (MQ=25)
                                                                             cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                        <  2:4881408/150‑1 (MQ=255)
                                                                               tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCCAACCCACACCCCAAACGGGCGCGGGGACCCGTCAGGACAGGATATATCTTGGCCGGGCTAACCacccat                                                                      >  2:2285954/1‑145 (MQ=19)
                                                                               tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  1:4996561/150‑1 (MQ=255)
                                                                               tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATACCGTACGTTACCCACATCCAAACCCTCACCCCAAAGGGGCGAGGGGACCCTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      >  1:375352/1‑150 (MQ=39)
                                                                                     ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                <  2:3429844/150‑1 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:5695976/150‑1 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:5438830/150‑1 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:1264396/150‑1 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:5561819/150‑1 (MQ=255)
                                                                                        tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:4152754/150‑1 (MQ=255)
                                                                                         aaGATGCGCCAGCTTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                            <  1:1034276/150‑1 (MQ=255)
                                                                                         aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                            <  2:5341538/150‑1 (MQ=255)
                                                                                          aGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCa                                                           <  1:5766618/150‑1 (MQ=255)
                                                                                                  cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                   <  1:5559149/150‑1 (MQ=255)
                                                                                                   aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGTCGAGGGGACCGTTCAGTACGGTATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCTATACTCTCa                                                  <  2:4547056/150‑1 (MQ=255)
                                                                                                   aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAAATTAGCTTGTCGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  >  2:927256/1‑150 (MQ=255)
                                                                                                        cgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaa                                             <  2:2399411/150‑1 (MQ=255)
                                                                                                            tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGCGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTTCTATACAGCATTAAATACTGGCAAGACTCACACGCAAAATc                                         >  2:133433/1‑150 (MQ=38)
                                                                                                              cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  1:5944624/150‑1 (MQ=255)
                                                                                                               ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATCCCGCATTAAATACTGGCAATACTCTAACGCAAAATCCgc                                      >  2:909795/1‑150 (MQ=39)
                                                                                                                gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                     <  1:6245387/150‑1 (MQ=255)
                                                                                                                 cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                                    <  1:4231526/150‑1 (MQ=255)
                                                                                                                 cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACAACCCAAAGGGGCCAGGGGGCCCGTCAGAAACGAAATTGGATTGAAGGTGGTATCCAGCAGTAAAAACTGGCAAATCAATCACGCCAAAACCgccg                                    >  2:1233075/1‑148 (MQ=255)
                                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCAAACCCCCAAGGGGCGAGGGGCCCCGTCAGTTCGGCAATTATCTTGACGGTGCTAACCAACAATAAAAAAAGGCAAAACGCTCCCCCAAAATACGCCGCAcccg                              >  2:4462031/1‑148 (MQ=255)
                                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGAGGGGCCCGTTCAGTACGGAAATTAGCTTTACGGTGCTATCCAGCAATAAATAATGGCAATACTCTCAAGCAAAATCCGCAGCAccct                              >  2:4147447/1‑148 (MQ=38)
                                                                                                                        ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                             <  2:2594158/150‑1 (MQ=255)
                                                                                                                                   ttttACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                  <  2:1941019/150‑1 (MQ=255)
                                                                                                                                   ttttACATCCCGTACGTTCCCCTCACCCTAAACCACTCCCCAAAGGGGCGAGGGGACCCTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                  >  2:1058238/1‑150 (MQ=255)
                                                                                                                                    tttACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                 <  1:5837483/150‑1 (MQ=255)
                                                                                                                                         aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt            <  1:2741929/150‑1 (MQ=255)
                                                                                                                                            ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg         <  1:3046814/150‑1 (MQ=255)
                                                                                                                                              gTACGTTCCCCTCACCCTAACCCTCAACCCAAACGGGCCAGGGGACCGTTCAGTACAGAATATAGCTTTACGGTGCTATCCAGCATTAAATAAAGGCAATAATCTCAAGCAAAATCCCCACCACCCTCTCTACACCGATAAGATTCGGGt       >  2:1776271/1‑150 (MQ=25)
                                                                                                                                                  gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  2:3317904/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504708‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: