Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,506 C→G 5.1% intergenic (‑140/+43) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,5060CG 5.1% 1541.0 / 24.9 457intergenic (‑140/+43)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (269/158);  new base G (3/27);  total (272/185)
Fisher's exact test for biased strand distribution p-value = 8.08e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 469 total aligned reads displayed.

CCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908357‑3908655
                                                                                                                                                     |                                                                                                                                                     
   tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgg                                                                                                                                                    <  1:460470/150‑1 (MQ=255)
            cAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  2:614783/1‑150 (MQ=255)
             aaTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGtt                                                                                                                                          >  2:6204882/1‑150 (MQ=255)
              aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                         <  2:1813221/150‑1 (MQ=255)
              aTCATTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTATCCTCTCCCCAGAGGGGCGAggggac                                                                                                                                         <  2:439033/150‑4 (MQ=38)
                cTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  1:4392061/150‑1 (MQ=255)
                cTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  2:2914683/150‑1 (MQ=255)
                 ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                      >  2:1636927/1‑150 (MQ=255)
                 ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                      >  1:2725684/1‑150 (MQ=255)
                     ccTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGATAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  <  2:787178/150‑1 (MQ=255)
                      cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  2:2686619/150‑1 (MQ=255)
                      cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  1:3870797/150‑1 (MQ=255)
                       tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                >  1:3162729/1‑150 (MQ=255)
                        cATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                                                                                               >  1:3454602/1‑150 (MQ=255)
                               ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCg                                                                                                                        >  2:3626557/1‑150 (MQ=255)
                               ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCg                                                                                                                        >  1:1659770/1‑150 (MQ=255)
                               ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCg                                                                                                                        >  2:4781337/1‑150 (MQ=255)
                                cTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgt                                                                                                                       >  1:2996078/1‑150 (MQ=255)
                                       aCTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCa                                                                                                                >  1:2156045/1‑150 (MQ=255)
                                        cTTCGCTAATCCGGCTTACCGTCGGCCGGATCCGGCGTTCCCGCCGCATCCGGCAATGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTCGGTCCCCTCGCCCCTGTGGGGAGCGGGTTTGGGTGCGGGGAAAACCGTGTCAGCaa                                                                                                               <  2:1332218/150‑1 (MQ=255)
                                         ttCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAat                                                                                                              >  2:1433105/1‑150 (MQ=255)
                                           cGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAACCGTGTCAGCAatat                                                                                                            >  1:3678891/1‑150 (MQ=255)
                                           cGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAatat                                                                                                            >  2:2515680/1‑150 (MQ=255)
                                              tAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCaa                                                                                                         >  1:6510109/1‑150 (MQ=255)
                                              tAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCaa                                                                                                         >  1:1814113/1‑150 (MQ=255)
                                                 tCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCg                                                                                                      >  2:5846723/1‑150 (MQ=255)
                                                    ggCTTACGGCCGGCCGGAGCAGGGGTTCACGCCGCATCCGGAAAAGTGTTAACCGTTAACCGAGTCAAAACGGCGCTGGGGGGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCgtgt                                                                                                   <  2:1118710/150‑1 (MQ=25)
                                                     gCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGtt                                                                                                  >  2:3074707/1‑150 (MQ=255)
                                                       ttACCGTCGGCCGGATCTGGCGTTCACGCCGCATCCGGCAATGTGTTAACCGTGTACAGCGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTa                                                                                                <  2:2647304/150‑1 (MQ=39)
                                                          ccGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTACCCTCGCCCCTCTGGGGCTAGGGTTAGGGTGAGGGGACAACCGTGTCAGCAATATCAACCGTGTTTAttc                                                                                             >  1:3921414/1‑150 (MQ=255)
                                                            gTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttctt                                                                                           <  2:4960828/150‑1 (MQ=255)
                                                             tcggCCGTATCTGGGGTTCACGCCGCATCGGTAAATGTTTTAACGGTTTACAGAGTAAAAACGGCGCTGGGTGGGTCCCCCCGCCCCTGTGGGGTGTGGTTTCGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                                                                                          <  2:1157058/148‑1 (MQ=255)
                                                                 ccGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa                                                                                      >  1:6111702/1‑150 (MQ=255)
                                                                 ccGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGATAACCGTGTACAGAGTCAAAAAGGCGCTATGTCTGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGACAGCAATATCAACAGTGTTTATTCTTCGCaa                                                                                      >  1:1559302/1‑150 (MQ=38)
                                                                      tCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaaca                                                                                 >  2:2425284/1‑150 (MQ=255)
                                                                        aGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaac                                                                               >  2:4298109/1‑150 (MQ=255)
                                                                         ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg                                                                              >  2:1462605/1‑150 (MQ=255)
                                                                          gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc                                                                             >  2:2068021/1‑150 (MQ=255)
                                                                          gCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgc                                                                             >  2:518864/1‑150 (MQ=255)
                                                                             ttCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcgcg                                                                          >  1:6589984/1‑150 (MQ=255)
                                                                              tCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACgcgcgc                                                                         >  2:5999964/1‑150 (MQ=255)
                                                                                aCGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCa                                                                       >  2:1610811/1‑150 (MQ=255)
                                                                                    cgcccccGGCAAAGTGTTACCCGTGTCCCGAGGCAAAAAGGCGCTCGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAAt                                                                   <  2:3159665/145‑1 (MQ=38)
                                                                                    cgcATCCGGAAACGTGTTACCCGTGTACAGAGTCAAAATGGCGCTCGGTGGGCCCCCTCGCCCCTCTGGGGAGTGGGTTCGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAAt                                                                   <  2:1220739/150‑1 (MQ=255)
                                                                                    cgaaTCCGGCAATGTTTTAACGGTGTACAGAGTCAAAATGGGGCTGGGTGGGTCCCCTCGCCCCTCTGGGTTGTGGGTTCGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAAt                                                                   <  2:5552029/147‑1 (MQ=255)
                                                                                       aTCCGGCAAAGTGTTAACCGTGTACAGCGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                                <  1:3990232/150‑1 (MQ=255)
                                                                                       aTCCGGAAATTTGTTAACGTTTTACATATTAAAAATGGCGCTGGGTCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtt                                                                <  1:1053873/150‑1 (MQ=37)
                                                                                        tCCGCCAATGTTATACCCGTGTACAGAGTAAAAACGGCGCTCGGGCGGCCCCCGCGCCCCTGGGGGTAGAGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTc                                                               <  1:5165058/150‑1 (MQ=255)
                                                                                         ccGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTca                                                              >  1:2575046/1‑150 (MQ=255)
                                                                                          cGGCAAAGTGTTAACCGTGTCCAGAGTCAAAATGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcag                                                             <  1:4877193/150‑1 (MQ=255)
                                                                                          cGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcag                                                             >  2:3814613/1‑150 (MQ=255)
                                                                                           ggCAATGTTTTAACCGTGTACAGAGTAAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGTAGTGGGTTAGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGtttgaa                                                            <  2:3046958/150‑5 (MQ=38)
                                                                                           ggCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagc                                                            >  1:1458585/1‑150 (MQ=255)
                                                                                             cAATGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcag                                                          <  1:6250091/150‑1 (MQ=255)
                                                                                              aaaGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTAAGAAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCTCGCAAGAATGTTcagaaga                                                         >  2:914120/1‑149 (MQ=38)
                                                                                               aaGTGTTAACCGTGTACAGCGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGTAGTGGTTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTcagcagca                                                        <  1:5820476/150‑1 (MQ=255)
                                                                                                  tgtTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTa                                                     >  2:5488295/1‑150 (MQ=255)
                                                                                                   gtTAACCGTGTACTGTGTAAAAACGGCGCTGGGTGGGTCCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTAc                                                    <  1:5443969/150‑1 (MQ=255)
                                                                                                      aaCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                                                 >  2:757854/1‑150 (MQ=255)
                                                                                                      aaCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGca                                                 >  1:4719511/1‑150 (MQ=255)
                                                                                                       aCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcac                                                >  1:5913056/1‑150 (MQ=255)
                                                                                                        ccGTGTACAGAGTCAAAACGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGCGGGTTCGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGcaca                                               <  1:4438007/150‑1 (MQ=255)
                                                                                                         cGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAg                                              >  2:5289805/1‑150 (MQ=255)
                                                                                                          gtgtACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAgg                                             >  1:5071806/1‑150 (MQ=255)
                                                                                                                 ttgtaaaaaaGGCGCTCGGTCGGTCCCCGCGCCCCTGTGGGGAGAGGGTTAGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGa                                      <  1:5373823/145‑1 (MQ=255)
                                                                                                                  agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     >  1:4826402/1‑150 (MQ=255)
                                                                                                                  agTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGAt                                     >  2:5662308/1‑150 (MQ=255)
                                                                                                                   gTCAAAACGGCGCGCGTCGGGCCCCCGCCCCCCTCTGGGGTGTGGTTTTGGGTGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATc                                    <  1:1269740/150‑1 (MQ=255)
                                                                                                                   gTCAAAAAGGCGCTCGGTCGGACCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATc                                    <  1:3308115/150‑1 (MQ=255)
                                                                                                                      aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTACTGATCAAt                                 >  2:5903274/1‑150 (MQ=255)
                                                                                                                      aaaaaGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAAt                                 >  1:5686172/1‑150 (MQ=255)
                                                                                                                            gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg                           >  2:3319512/1‑150 (MQ=255)
                                                                                                                            gcgcTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCg                           >  2:6463518/1‑150 (MQ=255)
                                                                                                                               gggggtcggccCCCTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGCAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                        <  1:2272574/147‑1 (MQ=255)
                                                                                                                               cTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGcc                        <  2:6392605/150‑1 (MQ=255)
                                                                                                                                tcggtcggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGccc                       >  2:5124499/1‑150 (MQ=255)
                                                                                                                                tcggtcggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCCAAAACAACGCGCGCCCGAATGTTCAGCAGCCGTACGCACAGGGTAATGAACAATACCCCGGccc                       >  1:1908006/1‑150 (MQ=39)
                                                                                                                                   ggcggtcCCCTCGCCCCTCTGGGGAGTGGGTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAgg                    <  1:3590033/148‑1 (MQ=255)
                                                                                                                                    tcggtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGc                   <  1:3203881/150‑1 (MQ=255)
                                                                                                                                       gtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTTAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCaa                <  2:4542055/150‑1 (MQ=255)
                                                                                                                                       gtcCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTGCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCaa                >  2:2752094/1‑150 (MQ=255)
                                                                                                                                           ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgc            >  2:1507939/1‑150 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGTGGGTTTGGTTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  2:1986700/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTCGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:744749/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  2:4003481/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgct           <  1:3069663/150‑1 (MQ=255)
                                                                                                                                            cTCGACCCTCTTGGGATAGGTTTAGTGTGAGTGGAAAACAGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGAAGTACGCACAGGGTAATGATAAATACCCCGGCCCATGACAATtgct           <  2:3918425/150‑1 (MQ=255)
                                                                                                                                             tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctg          >  2:3884611/1‑150 (MQ=255)
                                                                                                                                             tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctg          >  2:2186613/1‑150 (MQ=255)
                                                                                                                                             ctaacccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctg          >  2:23188/5‑150 (MQ=255)
                                                                                                                                              cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATtgctgc         <  2:3732876/150‑1 (MQ=255)
                                                                                                                                                ccccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCa       >  1:4147137/1‑150 (MQ=255)
                                                                                                                                                 cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAt      >  1:1865097/1‑150 (MQ=255)
                                                                                                                                                  ccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt     >  2:4115569/1‑150 (MQ=255)
                                                                                                                                                  ccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCAtt     <  2:1004554/150‑1 (MQ=255)
                                                                                                                                                   ctctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCGGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTc    <  1:2068021/150‑1 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   >  1:5595988/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTcc   <  1:6009468/150‑1 (MQ=255)
                                                                                                                                                     ctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCg  >  1:2408476/1‑150 (MQ=255)
                                                                                                                                                     ctGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCg  <  2:3532437/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCGCGCCAGAATGTTCAGCAGCAGTACGCACAGGGTAATGATCAATACCCCGGCCCAGGCCAATTGCTGCCATTCCG  >  NC_000913/3908357‑3908655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: