Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 6.1% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 6.1% 772.3 / 8.1 278L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (149/100);  new base T (2/26);  total (152/126)
Fisher's exact test for biased strand distribution p-value = 5.02e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

ATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238377‑238672
                                                                                                                                                  |                                                                                                                                                     
      atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  1:1501902/150‑1 (MQ=255)
      atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  1:579781/150‑1 (MQ=255)
      atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  1:3328444/150‑1 (MQ=255)
      atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  2:1052811/150‑1 (MQ=255)
      atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              >  1:889112/1‑150 (MQ=255)
       tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  1:4901647/150‑1 (MQ=255)
       tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  1:5196855/150‑1 (MQ=255)
       tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  1:3300026/150‑1 (MQ=255)
            tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:1972737/1‑150 (MQ=255)
              tgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAatat                                                                                                                                      >  2:22839/1‑150 (MQ=255)
               gACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAtt                                                                                                                                     >  2:3529911/1‑150 (MQ=255)
                  aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                  >  2:5713273/1‑150 (MQ=255)
                     aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  2:3761084/150‑1 (MQ=255)
                      gtatcTATTAAAGGTGCTATTACTATATCTATTAGTAAAAATATAAGAGTTAGATGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTTTCGTAAGTAAAATTTCATTAATATTTAAAAAg                                                                                                                              <  2:1339813/146‑1 (MQ=255)
                         gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                           <  1:4748140/150‑1 (MQ=255)
                          cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGaaa                                                                                                                          >  2:4359902/1‑150 (MQ=255)
                           tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                         >  2:2936660/1‑150 (MQ=255)
                              tAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGa                                                                                                                      <  2:6127467/150‑1 (MQ=255)
                                      cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                                              >  2:3583647/1‑150 (MQ=255)
                                        aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                            >  2:3384269/1‑150 (MQ=255)
                                             gATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGca                                                                                                       >  2:4812350/1‑150 (MQ=255)
                                             gATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGca                                                                                                       >  2:685077/1‑150 (MQ=255)
                                                  cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGaa                                                                                                  >  1:548959/1‑150 (MQ=255)
                                                   tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 >  2:6418991/1‑150 (MQ=255)
                                                   tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAAt                                                                                                 >  1:3662345/1‑150 (MQ=255)
                                                      tAGTAAAAATATAAGAGTTAGCTTTTTTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                              <  2:1849152/150‑1 (MQ=255)
                                                       aGTAAAAATATAAGAGTTAGCTTTATTGTTATGTCTGTTGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAATAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                             <  2:4715459/150‑1 (MQ=255)
                                                         tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                           >  2:275235/1‑150 (MQ=255)
                                                         tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACAAGAAACCGAAGCACAGAAacaacaa                                                                                           >  1:5637261/1‑149 (MQ=255)
                                                          aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAATCAACAtt                                                                                          >  1:5896698/1‑150 (MQ=255)
                                                           aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         >  2:1404911/1‑150 (MQ=255)
                                                           aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         >  2:1091943/1‑150 (MQ=255)
                                                           aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                         >  2:4555592/1‑150 (MQ=255)
                                                             aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                       <  2:3019612/150‑1 (MQ=255)
                                                              atatAAGTGTTCGCTTTTTTTTTTTGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAACAATGATTTTATTATCTTACTTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                                      <  1:2493693/150‑1 (MQ=255)
                                                                 taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAAACGAAGCACAGAATCAACATTCTCCAAt                                                                                   >  1:1012912/1‑150 (MQ=255)
                                                                      gTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAATTAAAATAATATTTAAAAAGGAAAACGACATGAAAAAGAAGCACAGAATAAAAAATCTCCAAtaataa                                                                              >  1:2124145/1‑150 (MQ=255)
                                                                       ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTATACAATCAaaaa                                                                             >  2:5098737/1‑150 (MQ=255)
                                                                         aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAATGAAAACGACAGGAAAACGAAGCACAGAAACAACAAAATACAATAATaaaaa                                                                           >  1:3515177/1‑149 (MQ=255)
                                                                         aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                           >  2:4924529/1‑150 (MQ=255)
                                                                            tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACATTATCCAAAAATAAAATAtt                                                                        >  1:435110/1‑150 (MQ=255)
                                                                            tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                                        >  2:2146549/1‑150 (MQ=255)
                                                                             gTATTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       <  2:3272168/150‑1 (MQ=255)
                                                                             gTATTGTTATGTCTGTGGCGAATTTGACTACATTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       <  1:1900111/150‑1 (MQ=255)
                                                                             gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       >  2:3665983/1‑150 (MQ=255)
                                                                             gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATAAACATTCTCCAAACATAAAATAttt                                                                       >  1:4222638/1‑150 (MQ=255)
                                                                              tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACAAGAAACCCAACCCCACAAACAACAAACTCCAATCAAAAAAAAATTc                                                                      >  1:4321636/1‑150 (MQ=255)
                                                                                    tATGTCTGTGGCTAAATTTACTACATTCGTTTTTTTGATTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                <  2:4767139/150‑1 (MQ=255)
                                                                                     aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                               >  2:5222370/1‑150 (MQ=255)
                                                                                       gTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAAAAAACAATCTCCAATAAAAAAATAATTACGTGGAGCa                                                             >  1:6456377/1‑150 (MQ=255)
                                                                                        tCTGTGGCGAAATTGACTACCTTCGTGTTTTTTATTTAGTATTATTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAATGAAAACGGCAAGAAAACCAAACACAGAATCAAAAATAAACAAAAATAAAATATTTACGTGGAGCAt                                                            >  1:5039596/1‑150 (MQ=255)
                                                                                          tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                          <  2:5545637/150‑1 (MQ=255)
                                                                                          tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGGAAGTAAACTTACAGGAATATTTAAAAAGGAAAACGACATGAACCCGAAGCACCGAAGCAACATTCTCCCAACATAAAAGATTTCCGGGGAGCAgtt                                                          >  2:741260/1‑150 (MQ=255)
                                                                                            tgGCGAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATAAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        <  1:113818/150‑1 (MQ=255)
                                                                                              gCGAAATTGACTACCTTCGTTTTTTTTTTTTATAATTATTTTATTATATTAAATAAAAATAAATGTATATTTAAAAATGAAAAAGACAATAAAACCAAGCACACAATAAAAATAATACAATAAAAAAATATTTAAGTGGGGCGTtttttt                                                      >  1:5955353/1‑150 (MQ=255)
                                                                                               cGAAATTGACTACCTTCGTGTTTTTTTTTAAGAATGATTTTATTATAGTAAGTAAAATTAAAATAATAATTAAAAATGAAAAAGACAATAAAACCAAGCACACAAAAAACAAAAACCAAAAAAAAAAAATTTAAGTGGAGGATTttatta                                                     >  1:5429514/1‑150 (MQ=255)
                                                                                                 aaaTTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  1:821622/150‑1 (MQ=255)
                                                                                                 aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  1:1625912/150‑1 (MQ=255)
                                                                                                   tttGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                 <  2:6088616/149‑1 (MQ=255)
                                                                                                   aTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                 <  2:2199861/150‑1 (MQ=255)
                                                                                                      gACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGTAGCATTTTATTATTGAata                                              >  2:1751568/1‑150 (MQ=255)
                                                                                                      gACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAata                                              >  2:6519533/1‑150 (MQ=255)
                                                                                                          aCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                          <  2:5096291/150‑1 (MQ=255)
                                                                                                          aCCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                                          <  1:2670821/150‑1 (MQ=255)
                                                                                                           ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  1:650957/150‑1 (MQ=255)
                                                                                                             ttCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACATGAATAATTAAAAAGGAAAAAGAAATGAAAACGAAGCACAGAATAAACAATATCCAAAAATAAAATATTTAAGTGGAGCATTTTATTATTTAAtatatatgt                                       >  1:738544/1‑145 (MQ=255)
                                                                                                             ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                       >  2:1662693/1‑150 (MQ=255)
                                                                                                              tCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTTAAAAGGAAAACGACATGAAACCGAAGCACAGAATAAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTTAATATATATGtt                                      >  1:799634/1‑150 (MQ=255)
                                                                                                               cttttttttGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                     <  2:2378402/148‑1 (MQ=255)
                                                                                                                gTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTa                                    >  2:2620412/1‑150 (MQ=255)
                                                                                                                 ttttttttATTAAGAATTATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                                   <  1:1247075/150‑1 (MQ=255)
                                                                                                                  ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:3809825/150‑1 (MQ=255)
                                                                                                                  ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  >  2:5911898/1‑150 (MQ=255)
                                                                                                                   tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACt                                 >  1:4072392/1‑150 (MQ=255)
                                                                                                                     tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               >  1:4438765/1‑150 (MQ=255)
                                                                                                                     tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               >  2:4639089/1‑150 (MQ=255)
                                                                                                                         aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  2:4021986/1‑150 (MQ=255)
                                                                                                                           tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         >  1:4032976/1‑150 (MQ=255)
                                                                                                                            aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                        >  2:4466029/1‑150 (MQ=255)
                                                                                                                            aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa                        >  1:142953/1‑150 (MQ=255)
                                                                                                                             aGAATGATTTTAGTATCTAAATTAAAATAACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                       <  1:6279837/150‑1 (MQ=255)
                                                                                                                                aTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa                    <  2:299954/150‑1 (MQ=255)
                                                                                                                                  taTTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:635775/149‑1 (MQ=255)
                                                                                                                                  taTTTTATTATCGTAAGTAAAATTACATGAATATATAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:940273/149‑1 (MQ=255)
                                                                                                                                    ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAGATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  2:5747864/1‑150 (MQ=255)
                                                                                                                                     tttATTATCTTAATTAAAATAACATTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               <  1:5556932/150‑1 (MQ=255)
                                                                                                                                     tttATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaa               <  2:803055/150‑1 (MQ=255)
                                                                                                                                      ttattaTCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGGAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaagaag              >  2:4119188/1‑150 (MQ=255)
                                                                                                                                         ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           <  2:390292/150‑1 (MQ=255)
                                                                                                                                         ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           >  1:2752847/1‑150 (MQ=255)
                                                                                                                                         ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           >  2:3418034/1‑150 (MQ=255)
                                                                                                                                          tatCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  1:2840918/150‑1 (MQ=255)
                                                                                                                                          tatCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  1:6120632/150‑1 (MQ=255)
                                                                                                                                          tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  2:3849609/150‑1 (MQ=255)
                                                                                                                                            tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa        >  1:3034522/1‑150 (MQ=255)
                                                                                                                                             agtaagtaaAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa       <  1:5286153/149‑1 (MQ=255)
                                                                                                                                              gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      >  1:3094061/1‑150 (MQ=255)
                                                                                                                                               taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     <  1:3719100/150‑1 (MQ=255)
                                                                                                                                                  gtaaAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg  <  2:2558199/150‑1 (MQ=255)
                                                                                                                                                  gtaaAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg  <  1:4667908/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238377‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: