Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,086,955 C→A 6.7% intergenic (+106/‑47) fdhD → / → yiiG formate dehydrogenase formation protein/DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,086,9550CA 6.7% 1157.1 / 4.1 390intergenic (+106/‑47)fdhD/yiiGformate dehydrogenase formation protein/DUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (85/237);  new base A (43/2);  total (151/239)
Fisher's exact test for biased strand distribution p-value = 5.08e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

GTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCT  >  NC_000913/4086806‑4087103
                                                                                                                                                     |                                                                                                                                                    
   gggCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAtt                                                                                                                                                   <  2:1902689/150‑1 (MQ=255)
    ggCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAAttt                                                                                                                                                  >  2:4498971/1‑150 (MQ=255)
      cAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATttat                                                                                                                                                <  1:6465973/150‑1 (MQ=255)
       aaCGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATttatt                                                                                                                                               <  1:5791728/150‑1 (MQ=255)
            tttATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGtt                                                                                                                                          <  2:623679/150‑1 (MQ=255)
                taCCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTATTTTTTAATTTATTAttttaaat                                                                                                                                      >  1:1433509/1‑146 (MQ=255)
                  cccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCActct                                                                                                                                    <  2:1846416/150‑1 (MQ=255)
                   ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTTTTATGTTCActctc                                                                                                                                   >  2:4423385/1‑150 (MQ=255)
                   ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCActctc                                                                                                                                   <  1:1099318/150‑1 (MQ=255)
                   ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCActctc                                                                                                                                   <  1:5557910/150‑1 (MQ=255)
                   ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTAAcactc                                                                                                                                   >  1:506119/1‑150 (MQ=255)
                   ccATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCTTTTTTCATTTATGATGTTCAcacgc                                                                                                                                   >  1:814384/1‑146 (MQ=255)
                     aTCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCtt                                                                                                                                 <  2:4997652/150‑1 (MQ=255)
                      tCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTa                                                                                                                                <  2:3410553/150‑1 (MQ=255)
                        cGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATc                                                                                                                              <  1:1421058/150‑1 (MQ=255)
                         gCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCt                                                                                                                             <  2:6045702/150‑1 (MQ=255)
                         gCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATTTTAACAATATTATCt                                                                                                                             >  1:2755730/1‑150 (MQ=255)
                           aGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtat                                                                                                                           <  2:2051219/150‑1 (MQ=255)
                             cGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCtatat                                                                                                                         <  1:6585856/150‑1 (MQ=255)
                               tttGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTGATTTATTATTTTGACTCTCTTATatatatat                                                                                                                       >  1:3146955/1‑150 (MQ=255)
                                 tGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATAttt                                                                                                                     <  1:1758203/150‑1 (MQ=255)
                                   aGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTtat                                                                                                                   >  2:223382/1‑150 (MQ=255)
                                     cAATTAATTAAAATTAGTCCTGCGAATTATTTTTAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATATACAATATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATaa                                                                                                                 <  2:3562776/150‑1 (MQ=255)
                                     cAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATaa                                                                                                                 <  2:3079277/150‑1 (MQ=255)
                                      aattaattAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAg                                                                                                                <  1:4463993/150‑1 (MQ=255)
                                      aattaattAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTCACTATCTTATCTATATATTTATaat                                                                                                                >  1:2134427/1‑149 (MQ=255)
                                       attaattaAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAgg                                                                                                               >  1:3870573/1‑150 (MQ=255)
                                          aattaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCaa                                                                                                            <  1:3979698/150‑1 (MQ=255)
                                           attaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAt                                                                                                           <  1:4516544/150‑1 (MQ=255)
                                           attaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAt                                                                                                           <  1:1015278/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          <  1:4399633/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          <  1:4920433/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          <  2:5984265/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          <  2:5231832/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          <  1:5957982/150‑1 (MQ=255)
                                            ttaaAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAAtt                                                                                                          >  1:3525530/1‑150 (MQ=255)
                                              aaaaTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTaa                                                                                                        >  2:2768958/1‑150 (MQ=255)
                                               aaaTATTTCCTGAGAAATATATTAAATTTCTGGAATTATTCATTTCGTGAATTTTATTATTTTATTTATAATCAAAATTCGTGTAGTGGCGTTTTCTTTTTTCATTTTTTTTGTTCACTCTCTTATCTATATATTTATAAGTCAATTaaa                                                                                                       <  2:934316/150‑1 (MQ=255)
                                                aaTAAGTCCTGCGATATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAAt                                                                                                      <  2:1741321/150‑1 (MQ=255)
                                                 aTAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATg                                                                                                     <  1:6299004/150‑1 (MQ=255)
                                                     gTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaaaa                                                                                                 <  1:1841733/150‑1 (MQ=255)
                                                          gCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTGACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAt                                                                                            >  2:378453/1‑150 (MQ=255)
                                                          gCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAATGAAt                                                                                            >  2:5714540/1‑150 (MQ=255)
                                                             aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATtta                                                                                         <  2:1359018/150‑1 (MQ=255)
                                                             aaaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAATGAATtta                                                                                         >  2:5400054/1‑150 (MQ=255)
                                                               atatatTAACTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttatt                                                                                       <  2:2795972/150‑1 (MQ=255)
                                                                  tatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATc                                                                                    <  1:6099862/150‑1 (MQ=255)
                                                                     tAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTc                                                                                 <  1:1257554/150‑1 (MQ=255)
                                                                     tAAATTGCAGGACTTATTCAATTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTTTTATGTTAACTAAATTAAATAAAAATATTTAAGGAAAAAAAAAGAAAAAGAAATTAATATAtta                                                                                 >  1:5385091/1‑149 (MQ=255)
                                                                      aaaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTATTATTTTGACTCTATTTTATATATATTTATAATTCAATTAAATTATAAGGAATTTATTATCttac                                                                                >  2:2381765/1‑148 (MQ=255)
                                                                            cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTAACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTACGCtatt                                                                          >  2:816683/1‑150 (MQ=255)
                                                                            cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAGTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                          >  2:1507484/1‑150 (MQ=255)
                                                                             aGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatta                                                                         <  1:4614439/150‑1 (MQ=255)
                                                                             aGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTGTTTTTTAATTTATTATTTTGACTCGCAGATGGATATATTTATAATGCAAATAAATTTAAATGAATTTATTATCTTAAGCtatta                                                                         >  1:1564838/1‑150 (MQ=255)
                                                                               gACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATtata                                                                       <  2:5354819/150‑1 (MQ=255)
                                                                                  ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                    <  1:4041199/150‑1 (MQ=255)
                                                                                  ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATTTTCACTCTATTATCTATATATTTATAAGGCAATTAAATGAAAATGAATTTATTATCTTACGCTAATATATTc                                                                    >  2:3990797/1‑150 (MQ=255)
                                                                                  ttATTCATTTCGTGAATTTTATTAGTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                    <  2:4075015/150‑1 (MQ=255)
                                                                                   tATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCg                                                                   <  1:4657523/150‑1 (MQ=255)
                                                                                    aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTACGCTCTTATAGTCGc                                                                  >  2:1005362/1‑150 (MQ=255)
                                                                                      tCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCca                                                                <  1:3360991/150‑1 (MQ=255)
                                                                                         tttCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcatca                                                             <  1:5694049/150‑1 (MQ=255)
                                                                                         tttCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATTTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcatca                                                             >  2:286582/1‑150 (MQ=255)
                                                                                           tCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATg                                                           <  2:6584667/150‑1 (MQ=255)
                                                                                            cGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGt                                                          <  1:899917/150‑1 (MQ=255)
                                                                                             gTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTATTTTGTTGACTCTCTTTTGTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTGCGGTATTATAGTGGCCATCATGTc                                                         >  2:2943197/1‑150 (MQ=255)
                                                                                              tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGGTTTTTTATGTTGACTCTCTTATGTATATATTTATAAGGCAATTAAATGAAAAGGTATTTATTATGTTCCGCTATTATATTCGGCAGCATGtgc                                                        >  2:5603656/1‑148 (MQ=255)
                                                                                              tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTcc                                                        <  2:3139428/150‑1 (MQ=255)
                                                                                               gAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATATTCGCCATCATGTccc                                                       >  2:2544729/1‑150 (MQ=255)
                                                                                                 attttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTc                                                     <  1:2844454/150‑1 (MQ=255)
                                                                                                 attttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTc                                                     <  2:1642717/150‑1 (MQ=255)
                                                                                                  ttttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCg                                                    <  1:4975870/150‑1 (MQ=255)
                                                                                                  ttttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCg                                                    <  1:218273/150‑1 (MQ=255)
                                                                                                  ttttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCg                                                    >  2:4233228/1‑150 (MQ=255)
                                                                                                   tttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCgg                                                   <  1:3259698/150‑1 (MQ=255)
                                                                                                    ttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGt                                                  <  2:5147691/150‑1 (MQ=255)
                                                                                                         attttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGAc                                             <  1:3665673/150‑1 (MQ=255)
                                                                                                          ttttatttATAAACACAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTTTTATAGTCGCCATCATGTCCCTCGGTCTGACg                                            <  1:1979634/150‑1 (MQ=255)
                                                                                                          ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTACGCTATTATAGTCGCCATCATGTCCCTCGGTCTGTCg                                            >  2:4694647/1‑150 (MQ=255)
                                                                                                           tttatttaTAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACgg                                           <  2:4801664/150‑1 (MQ=255)
                                                                                                               tttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATATTAGCCATCATTTCCCTCGGTCTGACGGGTtg                                       >  2:271445/1‑150 (MQ=255)
                                                                                                                ttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCAATTATTATGTTCACTCTCTTATCTATAAATTTATAAGGCAAATAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACggggggg                                      >  2:6493206/1‑146 (MQ=255)
                                                                                                                 tataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgtg                                     <  1:5876835/150‑1 (MQ=255)
                                                                                                                  ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                    <  2:5273269/150‑1 (MQ=255)
                                                                                                                   taAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtgat                                   <  1:255863/150‑1 (MQ=255)
                                                                                                                            tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                          <  2:2688688/150‑1 (MQ=255)
                                                                                                                            tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                          <  1:5539344/150‑1 (MQ=255)
                                                                                                                             gCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAg                         <  1:2656966/150‑1 (MQ=255)
                                                                                                                              cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                        <  1:6340426/150‑1 (MQ=255)
                                                                                                                               gtgtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGcc                       <  1:1885950/150‑1 (MQ=255)
                                                                                                                                    gTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAAc                  <  1:5166159/150‑1 (MQ=255)
                                                                                                                                     tGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCGTATCTATATATTTATAAGGCAATTAAAAGAAAAGGAATTTATTATCTTCCGCTATTATAGTCCCCATCATGTCCCACGGTCGGACGGGTTGTGATGATAAAAAAGCCGAAAcc                 >  2:6390515/1‑149 (MQ=255)
                                                                                                                                         gTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaaa             <  2:1203315/150‑1 (MQ=255)
                                                                                                                                         gTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaaa             <  2:1989665/150‑1 (MQ=255)
                                                                                                                                         gTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTGATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGTGTTGTGATGATAAAAAAGCCGAAACAGaaa             <  1:3778776/150‑1 (MQ=255)
                                                                                                                                          ttttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCGCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAGAc            >  2:4273706/1‑150 (MQ=255)
                                                                                                                                             tCTTTTTTCATTTATTATGTTCACTCTATTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCt         <  1:4237846/150‑1 (MQ=255)
                                                                                                                                              cTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTc        >  1:2907254/1‑150 (MQ=255)
                                                                                                                                              cTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTc        >  2:2187096/1‑150 (MQ=255)
                                                                                                                                                tttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTccc      <  2:3267657/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCGGGCAACGGTTTATACCCATCCGCAGCGTTTGAGCAATTAATTAAAATAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCT  >  NC_000913/4086806‑4087103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: