BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,612,241	G→T	6.2%	G38G (GGG→GGT)	ytjA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,241	0	G	T	6.2%	1201.9 / 14.9	353	G38G (GGG→GGT)	ytjA	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (197/118); new base T (2/35); total (199/154)
Fisher's exact test for biased strand distribution p-value = 4.95e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

AAGCGGTGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACA  >  NC_000913/4612093‑4612390

aaGCGGTGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGa                                                                                                                                                      >  2:808448/1‑150 (MQ=255)

 aGCGGTGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGat                                                                                                                                                     >  1:706539/1‑150 (MQ=255)

     gTGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGattatt                                                                                                                                                 <  2:1838937/150‑1 (MQ=255)

      tGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTc                                                                                                                                                <  1:1547028/150‑1 (MQ=255)

       gACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCt                                                                                                                                               <  1:6077563/150‑1 (MQ=255)

       gACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCt                                                                                                                                               <  2:3771179/150‑1 (MQ=255)

       gACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCt                                                                                                                                               <  2:464692/150‑1 (MQ=255)

        aCATTAACTATGGTAAAGGAGACGCTTTTGTTTCGTTGGGGCGTCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGGTTGGTGGGGGGGGCGGTACCGCTGCAGGCGCAGCTAAAATTGTGTTTGTCGTCGGGATTATTATg                                                                                                                                              >  1:561668/1‑150 (MQ=255)

          aTTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTAGTCGGGATTATTCTGtt                                                                                                                                            >  1:4581813/1‑150 (MQ=255)

           ttAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTc                                                                                                                                           >  2:2560812/1‑150 (MQ=255)

            tAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTcc                                                                                                                                          <  1:5179881/150‑1 (MQ=255)

            tAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTcc                                                                                                                                          >  2:3608659/1‑150 (MQ=255)

                tATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGattatt                                                                                                                                                 <  2:4002635/139‑1 (MQ=255)

                tATGGTAAAGGAGACGCTTATGTTTAGTTGGGGCATCATATTTCTGGGTATCGCGTTAATCGCCCCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGt                                                                                                                                      >  1:6021158/1‑150 (MQ=255)

                  tGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGa                                                                                                                                    <  1:1700313/150‑1 (MQ=255)

                   ggTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAg                                                                                                                                   >  2:162955/1‑150 (MQ=255)

                    gTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGt                                                                                                                                  >  2:55180/1‑150 (MQ=255)

                    gTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGt                                                                                                                                  >  2:2448319/1‑150 (MQ=255)

                    gTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTGTGTCGTCGGGATTATTCTGTTCCTGGTGAGt                                                                                                                                  >  2:4373870/1‑150 (MQ=255)

                     tAAAGGAGACGCTTATGTTTCGTTTGGGCATCATATTTATGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTTGTATGGCCGGTAACGCTGCAGGCGCAGCTAAAAATGTCTTTGTAGTCGGGATTATTCTGTTACTGGTGAttt                                                                                                                                 >  1:3573707/1‑150 (MQ=255)

                     tAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGtt                                                                                                                                 <  2:6054788/150‑1 (MQ=255)

                       aaGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTg                                                                                                                               <  2:2279049/150‑1 (MQ=255)

                         ggAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGtt                                                                                                                             <  1:4169209/150‑1 (MQ=255)

                         ggAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGtt                                                                                                                             <  2:634480/150‑1 (MQ=255)

                           agaCGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCa                                                                                                                           <  1:6241786/150‑1 (MQ=255)

                               gCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATggg                                                                                                                       >  2:3557289/1‑150 (MQ=255)

                                 ttATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGcc                                                                                                                     <  1:3677654/150‑1 (MQ=255)

                                 ttATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGcc                                                                                                                     <  2:3643184/150‑1 (MQ=255)

                                 ttATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGcc                                                                                                                     <  2:4067292/150‑1 (MQ=255)

                                          gTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAc                                                                                                            >  1:2444463/1‑150 (MQ=255)

                                          gTTGGGGAATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGATGCAGGCGCAGCTAAATTTGTCTTTGTCGTCGGGATTTTTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAc                                                                                                            <  2:2245399/150‑1 (MQ=255)

                                           ttGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAcc                                                                                                           >  2:5371273/1‑150 (MQ=255)

                                           ttGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAcc                                                                                                           >  2:1763912/1‑150 (MQ=255)

                                           ttGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAcc                                                                                                           >  1:2935873/1‑150 (MQ=255)

                                           ttGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGAAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGAcc                                                                                                           >  2:3334483/1‑150 (MQ=255)

                                           ttGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCAGGCCCGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCCGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGACCGAAAACGAcc                                                                                                           >  2:6223955/1‑150 (MQ=255)

                                            tGGGGAATCATTTTTCTGGTTATCGCTTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGATAAATTGGTCTTTGTCGTGGGTATTATTCTTTTCCTGGTTTTTTTTTTCATGGGCCGAAAACGAccc                                                                                                          <  2:273408/150‑1 (MQ=255)

                                              gggCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTa                                                                                                        >  2:3408121/1‑150 (MQ=255)

                                                 catcatATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGAt                                                                                                     >  2:559055/1‑150 (MQ=255)

                                                 catcatATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGAt                                                                                                     >  2:3919074/1‑150 (MQ=255)

                                                     atttttCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGGGGTGTGGCCGGTACCGCTGCAGGCGCAGATAAATTTGTATTTGTCGTGGGTATTATTCTTTTCCTGGTGAGTTTGTTCATGGGCCTAAAACGACCCTAGATTTCa                                                                                                 <  2:1688752/147‑1 (MQ=255)

                                                     atatTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGttt                                                                                                                                <  2:473787/119‑1 (MQ=255)

                                                       ttttCTGGTTTTCTCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTGTGGCCGGTACCGCTGCTGGCGCAGATTAATTTGTCTTTGTCGTCGGGTTTATTCTGTTCCTGGTTTTTTTGTTCATGGGCCTAAAACGACCCTAGATTTCata                                                                                               <  2:185749/149‑3 (MQ=255)

                                                       atTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGa                                                                                               >  1:5412164/1‑150 (MQ=255)

                                                        tttCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGAc                                                                                              <  2:3472031/150‑1 (MQ=255)

                                                             ggTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTa                                                                                         >  1:5002238/1‑150 (MQ=255)

                                                             ggTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTa                                                                                         >  1:6394260/1‑150 (MQ=255)

                                                                  tCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGaaa                                                                                    >  2:2084289/1‑150 (MQ=255)

                                                                   cgcgTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGaaaa                                                                                   >  1:2079576/1‑150 (MQ=255)

                                                                   cgcgTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGaaaa                                                                                   >  1:816918/1‑150 (MQ=255)

                                                                    gcgTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAAt                                                                                  >  2:3170674/1‑150 (MQ=255)

                                                                     cgTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAAtt                                                                                 >  2:2971756/1‑150 (MQ=255)

                                                                     cgTTAATCGCCGCCGCACGTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAAtt                                                                                 >  2:1377560/1‑150 (MQ=255)

                                                                       ttAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAAtttt                                                                               >  1:1225898/1‑150 (MQ=255)

                                                                        tAATCGCCGCCGCAGTGGGTTTTGGTGGGGTGGCGGGTCCCGCTGCCGGCGCCGCTAAATTTGCATTTGTCTTGGGGATTTTTCTGTTCCTGTTGTGTTTTTTCTTGGCCCTAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTa                                                                              <  1:5912426/150‑1 (MQ=255)

                                                                        tAATCGCCGCCGCACTGGGGTTTGGTGGTCTGGCCGGTACCGCGGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTTTTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTa                                                                              <  2:5080882/150‑1 (MQ=255)

                                                                          aTCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATc                                                                            >  1:5521887/1‑150 (MQ=255)

                                                                                gccgcTGTTGGGTTGGGTGGTCTGGCCGTCACCGCGGCCGGCGCAGATAAAATTCTCTTTGTCGTCGGTATTATTCTGTTCGGGGTGTTTTTGTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGGAAATTTTATCCAAAGc                                                                      <  1:3903369/150‑1 (MQ=255)

                                                                                gccgcACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCAGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGc                                                                      <  2:4581813/150‑1 (MQ=255)

                                                                                 ccgcACTTGGGTTTGGTGGTCTGGCCGGTACCGATGCAGGCGCAGCTAAAATTGTCTTTGTCGACGGGATTATTCTGTTCCTGGTGAGTTTGTACATGGGCCGAAAACGACACTAGATTTCAGACCATTAAGAAAATTATATCCAAAGcc                                                                     >  2:4222309/1‑150 (MQ=255)

                                                                                       ttGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGTGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGACAACGACACTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                               >  1:5013943/1‑150 (MQ=255)

                                                                                       ttGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                               >  2:5312647/1‑150 (MQ=255)

                                                                                       ttGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                               >  2:903071/1‑150 (MQ=255)

                                                                                       ttGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCa                                                               >  1:1349492/1‑150 (MQ=255)

                                                                                         gggTTGGGTGGTCTGGCCGGTACCGCTGCCGGCGCAGCTAAAATTGTCTTTGTCGTGGGTATTTTTCTGTTCCTGGTGAGTTTGTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGc                                                             <  1:4032612/150‑1 (MQ=255)

                                                                                               ggtggtCGGGCGGGTCCGGCTGCTGGCGCCGCTAAATTTGTCTTTGTCGTCGGTTTTTTTCTGTTCGTGGTGATTTTGTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTg                                                       <  1:2729730/150‑1 (MQ=255)

                                                                                                gtggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCCTTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTgg                                                      >  1:6539073/1‑150 (MQ=255)

                                                                                                gtggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTgg                                                      >  2:3821104/1‑150 (MQ=255)

                                                                                                gtggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTgg                                                      <  2:4923886/150‑1 (MQ=255)

                                                                                                 tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTCAGAAAAGTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                     >  2:5983126/1‑150 (MQ=255)

                                                                                                    tCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCttt                                                  >  1:4425965/1‑150 (MQ=255)

                                                                                                      tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGTATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                >  1:785777/1‑150 (MQ=255)

                                                                                                        gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                              >  2:1034708/1‑150 (MQ=255)

                                                                                                        gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                              >  1:6373814/1‑150 (MQ=255)

                                                                                                        gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                              >  1:2186620/1‑150 (MQ=255)

                                                                                                        gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTGTTGCg                                              >  1:1491906/1‑150 (MQ=255)

                                                                                                            gcaCCGCCGCCGGCGCCGATAAAATTGTCTTGGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTTTACATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGttt                                          <  1:2750749/148‑1 (MQ=255)

                                                                                                              cccGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTGGGTATTATTCTGTTCCTGGTGATTTTGTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTa                                        <  1:1064741/149‑1 (MQ=255)

                                                                                                              aCCGCTCCTGCCCCAGCTAAATTTGTCTTTGTCGTGGGGATTTTTCTGTGCGTGGTGAGTTTGTCCAGGGGCCGAACACGACCCTAGATGTCAGACCATTAAGAAAATTTTATCCAAAGCCTGTCCAGCGGACTGGCTTTTGCGGTTTTa                                        <  1:568463/150‑1 (MQ=255)

                                                                                                                 gCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCg                                     >  2:5744356/1‑150 (MQ=255)

                                                                                                                 gCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGCg                                     >  1:1391806/1‑150 (MQ=255)

                                                                                                                      aGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaataa                                >  2:4456190/1‑150 (MQ=255)

                                                                                                                        gcgcCGATAAACTTGTCTTTGGCGTGGGTATTATTGTGTTCCTGGTGTGTTTGTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAataata                              <  2:5009249/150‑1 (MQ=255)

                                                                                                                             gataattttGTCTTGGTCGTCGGTATTTTTCTGTTCCTGGTGAGTTTGTTCAGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTg                         <  2:1459181/143‑1 (MQ=255)

                                                                                                                               tAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                       >  1:896303/1‑150 (MQ=255)

                                                                                                                                     tGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACtt                 <  1:335131/150‑1 (MQ=255)

                                                                                                                                       tCTTTGTCGTCGGGATTATTCTGTTCATGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCAAGCGGACTGGCTTTTGCGGTTTTAGCGAATCATAAATTGCGTTACTTTg               >  1:5280625/1‑150 (MQ=255)

                                                                                                                                        cTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt              >  1:1729120/1‑150 (MQ=255)

                                                                                                                                         tttGTCGTCGGTATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc             <  1:196374/150‑1 (MQ=255)

                                                                                                                                         tttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc             >  2:5367122/1‑150 (MQ=255)

                                                                                                                                             tcgtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTc         <  1:1196109/150‑1 (MQ=255)

                                                                                                                                              cgtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCt        <  1:4876165/150‑1 (MQ=255)

                                                                                                                                                tcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCttt      >  1:4749138/1‑150 (MQ=255)

                                                                                                                                                tcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCttt      <  2:676047/150‑1 (MQ=255)

                                                                                                                                                tcgGGATTATTCTGTTCCGGGTGTGTTTGTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCttt      <  2:5141962/150‑1 (MQ=255)

                                                                                                                                                 cgGGATTATTCTGTTCCTGTTGAGTTGTTCCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc     <  1:3729025/150‑1 (MQ=255)

                                                                                                                                                 cgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTc     <  1:426128/150‑1 (MQ=255)

                                                                                                                                                    gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcaca  <  1:2536369/150‑1 (MQ=255)

                                                                                                                                                    gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTGTcaca  >  1:3485433/1‑150 (MQ=255)

                                                                                                                                                    gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCGAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcaca  >  2:5919184/1‑150 (MQ=255)

AAGCGGTGACATTAACTATGGTAAAGGAGACGCTTATGTTTCGTTGGGGCATCATATTTCTGGTTATCGCGTTAATCGCCGCCGCACTTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACA  >  NC_000913/4612093‑4612390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`